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1.
Proc Natl Acad Sci U S A ; 115(36): 9014-9019, 2018 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-30126999

RESUMO

Abnormally enlarged early endosomes (EEs) are pathological features of neurodegenerative diseases, yet insight into the mechanisms and consequences of EE expansion remains elusive. Here, we report swollen apical EEs in the retinal pigment epithelium (RPE) of aged human donors and in the pigmented Abca4-/- mouse model of Stargardt early-onset macular degeneration. Using high-resolution live-cell imaging, we show that age-related and pathological accumulation of lipofuscin bisretinoids increases ceramide at the apical surface of the RPE, which promotes inward budding and homotypic fusion of EEs. These enlarged endosomes internalize the complement protein C3 into the RPE, resulting in the intracellular generation of C3a fragments. Increased C3a in turn activates the mechanistic target of rapamycin (mTOR), a regulator of critical metabolic processes such as autophagy. The antidepressant desipramine, which decreases ceramide levels by inhibiting acid sphingomyelinase, corrects EE defects in the RPE of Abca4-/- mice. This prevents C3 internalization and limits the formation of C3a fragments within the RPE. Although uncontrolled complement activation is associated with macular degenerations, how complement contributes to pathology in a progressive disease is not well understood. Our studies link expansion of the EE compartment with intracellular complement generation and aberrant mTOR activation, which could set the stage for chronic metabolic reprogramming in the RPE as a prelude to disease. The pivotal role of ceramide in driving EE biogenesis and fusion in the Abca4-/- mice RPE suggests that therapeutic targeting of ceramide could be effective in Stargardt disease and other macular degenerations.


Assuntos
Complemento C3a/metabolismo , Endossomos/metabolismo , Degeneração Macular/congênito , Epitélio Pigmentado da Retina/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Transportadores de Cassetes de Ligação de ATP/deficiência , Idoso , Idoso de 80 Anos ou mais , Animais , Ceramidas/genética , Ceramidas/metabolismo , Complemento C3a/genética , Modelos Animais de Doenças , Endossomos/genética , Endossomos/patologia , Feminino , Humanos , Degeneração Macular/genética , Degeneração Macular/metabolismo , Degeneração Macular/patologia , Masculino , Camundongos , Camundongos Knockout , Epitélio Pigmentado da Retina/patologia , Doença de Stargardt , Suínos , Serina-Treonina Quinases TOR/genética
2.
Cerebellum ; 15(1): 10-13, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26559892

RESUMO

The control of deep cerebellar nuclear (DCN) neuronal firing is central to cerebellar function but is not well understood. The large majority of synapses onto DCN neurons derive from Purkinje cells (PCs), suggesting that PC activity is an important determinant of DCN firing; however, PCs fire both simple and complex spikes (CSs), and little is known about how the latter's action affects DCN activity. Thus, here, we explored the effects of CSs on DCN activity. CSs were recorded from PC arrays along with individual DCN neurons. Presumed synaptically connected PC-DCN cell pairs were identified using CS-triggered correlograms of DCN activity, which also showed that CS activity was associated with a predominantly inhibitory effect on DCN activity. The strength of the CS effect varied as a function of synchrony, such that isolated CSs produced only weak inhibition of DCN activity, whereas highly synchronous CSs caused a larger drop in firing levels. Although the present findings were obtained in anesthetized animals, similar CS synchrony levels exist in awake animals, and changes in synchrony level have been observed in association with movements in awake animals. Thus, the present data suggest that synchronous CS activity may be a mechanism for shaping DCN output related to motor commands.


Assuntos
Potenciais de Ação/fisiologia , Núcleos Cerebelares/citologia , Rede Nervosa/fisiologia , Inibição Neural/fisiologia , Células de Purkinje/fisiologia , Animais , Estimulação Elétrica , Feminino , Ratos , Ratos Sprague-Dawley
3.
Adv Exp Med Biol ; 854: 731-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26427482

RESUMO

Transient expression of exogenous proteins facilitates studies of molecular mechanisms and utility for transplantation of retinal pigment epithelial (RPE) cells in culture. Here, we compared expression of the membrane protein ß5 integrin-GFP (ß5-GFP) in two recently established models of differentiated human RPE, adult RPE stem cell-derived RPE and primary fetal RPE, upon infection with recombinant adenovirus or transfection with DNA in liposomes. We varied viral titer and duration of virus incubation and examined ß5-GFP and the tight junction marker ZO-1 in manipulated cells by confocal microscopy. Fewer than 5 % of cells expressed ß5-GFP after liposome-mediated transfection. The percentage of cells with detectable ß5-GFP exceeded 90 % after adenovirus infection for as little as 1 h. Decreasing virus titer two-fold did not alter the fraction of cells expressing ß5-GFP but increased variability of ß5-GFP level among cells. In cells with low expression levels, ß5-GFP localized mostly to the apical plasma membrane like endogenous αvß5 integrin. In cells with high expression levels, ß5-GFP localized to the cytoplasm in addition to the apical surface suggesting accumulation in trafficking compartments. Altogether, adenovirus delivery yields efficient exogenous membrane protein expression of correct polarity in differentiated human RPE cells in culture.


Assuntos
Adenoviridae/genética , Proteínas de Membrana/genética , Epitélio Pigmentado Ocular/metabolismo , Polaridade Celular , Células Cultivadas , Células Epiteliais/metabolismo , Vetores Genéticos/genética , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Humanos , Cadeias beta de Integrinas/genética , Cadeias beta de Integrinas/metabolismo , Lipossomos , Proteínas de Membrana/metabolismo , Microscopia Confocal , Epitélio Pigmentado Ocular/citologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Reprodutibilidade dos Testes , Transfecção/métodos
4.
Stem Cell Res Ther ; 14(1): 53, 2023 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-36978104

RESUMO

National Eye Institute recently issued a new Strategic Plan outlining priority research areas for the next 5 years. Starting cell source for deriving stem cell lines is as an area with gaps and opportunities for making progress in regenerative medicine, a key area of emphasis within the NEI Strategic Plan. There is a critical need to understand how starting cell source affects the cell therapy product and what specific manufacturing capabilities and quality control standards are required for autologous vs allogeneic stem cell sources. With the goal of addressing some of these questions, in discussion with the community-at-large, NEI hosted a Town Hall at the Association for Research in Vision and Ophthalmology annual meeting in May 2022. This session leveraged recent clinical advances in autologous and allogeneic RPE replacement strategies to develop guidance for upcoming cell therapies for photoreceptors, retinal ganglion cells, and other ocular cell types. Our focus on stem cell-based therapies for RPE underscores the relatively advanced stage of RPE cell therapies to patients with several ongoing clinical trials. Thus, this workshop encouraged lessons learned from the RPE field to help accelerate progress in developing stem cell-based therapies in other ocular tissues. This report provides a synthesis of the key points discussed at the Town Hall and highlights needs and opportunities in ocular regenerative medicine.


Assuntos
Células-Tronco Pluripotentes Induzidas , Células-Tronco Pluripotentes , Doenças Retinianas , Humanos , Doenças Retinianas/terapia , Doenças Retinianas/metabolismo , Transplante de Células-Tronco , Terapia Baseada em Transplante de Células e Tecidos , Células-Tronco Pluripotentes Induzidas/metabolismo , Epitélio Pigmentado da Retina/metabolismo
5.
J Neurosci ; 31(41): 14708-20, 2011 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-21994387

RESUMO

Cerebellar output is necessary for the ideal implementation of many nervous system functions, particularly motor coordination. A key step toward understanding the generation of this output is characterizing the factors that shape the activity of the cerebellar nuclei (CN). There are four major sources of synaptic input that modulate CN activity; collaterals of climbing and mossy fibers are two, and the remaining two are provided by Purkinje cell (PC) axons in the form of simple spikes (SSs) and complex spikes (CSs). Most hypotheses of cerebellar function focus on SSs as the primary determinant of CN activity. However, it is likely that CSs also cause significant direct effects on CN activity, something that is rarely considered. To explore this possibility, we recorded from synaptically connected PC-CN neuron cell pairs in rats. Cross-correlograms of CS and CN activity from such recordings demonstrate that spontaneous CSs have a strong inhibitory effect on CN activity, apparently sufficient, in some cases, to trigger changes in the intrinsic excitability of the CN neuron that long outlast the underlying CS-mediated GABAergic IPSP. Furthermore, many CS-CN correlograms show an initial excitatory response, demonstrating the ability of climbing fiber collaterals to significantly excite CN neurons. A substantial fraction (24%) of correlograms displayed an excitation-inhibition sequence, providing evidence that a CN neuron often receives collaterals from the same olivocerebellar axons as innervate the PCs projecting to it. Thus, excitation followed by inhibition appears to be a hard-wired response pattern of many CN neurons to olivocerebellar activity.


Assuntos
Potenciais de Ação/fisiologia , Córtex Cerebelar/fisiologia , Núcleos Cerebelares/citologia , Neurônios/fisiologia , Núcleo Olivar/fisiologia , Transmissão Sináptica/fisiologia , Potenciais de Ação/efeitos dos fármacos , Vias Aferentes/fisiologia , Analgésicos/farmacologia , Animais , Mapeamento Encefálico , Estimulação Elétrica , Feminino , Ketamina/farmacologia , Modelos Neurológicos , Inibição Neural/efeitos dos fármacos , Inibição Neural/fisiologia , Neurônios/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Transmissão Sináptica/efeitos dos fármacos , Xilazina/farmacologia
6.
STAR Protoc ; 3(2): 101383, 2022 06 17.
Artigo em Inglês | MEDLINE | ID: mdl-35664254

RESUMO

Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).


Assuntos
COVID-19 , Adulto , Olho , Humanos , Organoides , SARS-CoV-2
7.
Front Cell Dev Biol ; 10: 910040, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36092714

RESUMO

The therapeutic potential of pluripotent stem cells is great as they promise to usher in a new era of medicine where cells or organs may be prescribed to replace dysfunctional tissue. At the forefront are efforts in the eye to develop this technology as it lends itself to in vivo monitoring and sophisticated non-invasive imaging modalities. In the retina, retinal pigment epithelium (RPE) is the most promising replacement cell as it has a single layer, is relatively simple to transplant, and is associated with several eye diseases. However, after transplantation, the cells may transform and cause complications. This transformation may be partially due to incomplete maturation. With the goal of learning how to mature RPE, we compared induced pluripotent stem cell-derived RPE (iPSC-RPE) cells with adult human primary RPE (ahRPE) cells and the immortalized human ARPE-19 line. We cultured ARPE-19, iPSC-RPE, and ahRPE cells for one month, and evaluated morphology, RPE marker staining, and transepithelial electrical resistance (TEER) as quality control indicators. We then isolated RNA for bulk RNA-sequencing and DNA for genotyping. We genotyped ahRPE lines for the top age-related macular degeneration (AMD) and proliferative vitreoretinopathy (PVR) risk allele polymorphisms. Transcriptome data verified that both adult and iPSC-RPE exhibit similar RPE gene expression signatures, significantly higher than ARPE-19. In addition, in iPSC-RPE, genes relating to stem cell maintenance, retina development, and muscle contraction were significantly upregulated compared to ahRPE. We compared ahRPE to iPSC-RPE in a model of epithelial-mesenchymal transition (EMT) and observed an increased sensitivity of iPSC-RPE to producing contractile aggregates in vitro which resembles incident reports upon transplantation. P38 inhibition was capable of inhibiting iPSC-RPE-derived aggregates. In summary, we find that the transcriptomic signature of iPSC-RPE conveys an immature RPE state which may be ameliorated by targeting "immature" gene regulatory networks.

8.
Nat Commun ; 13(1): 2796, 2022 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-35589753

RESUMO

One common cause of vision loss after retinal detachment surgery is the formation of proliferative and contractile fibrocellular membranes. This aberrant wound healing process is mediated by epithelial-mesenchymal transition (EMT) and hyper-proliferation of retinal pigment epithelial (RPE) cells. Current treatment relies primarily on surgical removal of these membranes. Here, we demonstrate that a bio-functional polymer by itself is able to prevent retinal scarring in an experimental rabbit model of proliferative vitreoretinopathy. This is mediated primarily via clathrin-dependent internalisation of polymeric micelles, downstream suppression of canonical EMT transcription factors, reduction of RPE cell hyper-proliferation and migration. Nuclear factor erythroid 2-related factor 2 signalling pathway was identified in a genome-wide transcriptomic profiling as a key sensor and effector. This study highlights the potential of using synthetic bio-functional polymer to modulate RPE cellular behaviour and offers a potential therapy for retinal scarring prevention.


Assuntos
Fator 2 Relacionado a NF-E2 , Epitélio Pigmentado da Retina , Animais , Linhagem Celular , Movimento Celular , Cicatriz/metabolismo , Transição Epitelial-Mesenquimal , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Polímeros/metabolismo , Coelhos , Epitélio Pigmentado da Retina/metabolismo
9.
Cerebellum ; 10(4): 694-701, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21373863

RESUMO

The question of what modulates the firing of the cerebellar nuclei (CN) is one to which we presently have a surprisingly incomplete answer. Because most synaptic input to the CN originates from Purkinje cells (PCs), and simple spikes (SSs) are far more numerous than complex spikes (CSs), SSs are generally thought to be the dominant influence on the CN. However, evidence, reviewed here, suggests that this appears not to be the case in some physiologically important situations. As an alternative, we propose that CS activity may have at least as significant an effect on CN firing as do SSs. In particular, we suggest that CS activity has a role in controlling the bursts CN neurons show during various movements, during sleep states, and under ketamine-xylazine anesthesia. The ability to perform this role rests on the fact that CSs can be highly synchronized among PCs that project to the same CN neuron. Specifically, we suggest that synchronized CSs help determine the temporal course of the CN bursts, most often their offset, and that SSs and activity from cerebellar afferents may modulate the specific firing pattern within each burst. This joint control of CN activity may help explain anomalies present in the standard model for synaptic control of CN activity in which determination of CN firing patterns is attributed primarily to SSs.


Assuntos
Potenciais de Ação/fisiologia , Núcleos Cerebelares/citologia , Núcleos Cerebelares/fisiologia , Vias Aferentes/fisiologia , Animais , Humanos , Células de Purkinje/fisiologia
10.
Cell Stem Cell ; 28(7): 1205-1220.e7, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34022129

RESUMO

The SARS-CoV-2 pandemic has caused unparalleled disruption of global behavior and significant loss of life. To minimize SARS-CoV-2 spread, understanding the mechanisms of infection from all possible routes of entry is essential. While aerosol transmission is thought to be the primary route of spread, viral particles have been detected in ocular fluid, suggesting that the eye may be a vulnerable point of viral entry. To this end, we confirmed SARS-CoV-2 entry factor and antigen expression in post-mortem COVID-19 patient ocular surface tissue and observed productive viral replication in cadaver samples and eye organoid cultures, most notably in limbal regions. Transcriptional analysis of ex vivo infected ocular surface cells and hESC-derived eye cultures revealed robust induction of NF-κB in infected cells as well as diminished type I/III interferon signaling. Together these data suggest that the eye can be directly infected by SARS-CoV-2 and implicate limbus as a portal for viral entry.


Assuntos
COVID-19 , Células-Tronco Embrionárias Humanas , Adulto , Epitélio , Humanos , Pandemias , SARS-CoV-2
11.
Nat Commun ; 12(1): 5675, 2021 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-34584087

RESUMO

The retina is a widely profiled tissue in multiple species by single-cell RNA sequencing studies. However, integrative research of the retina across species is lacking. Here, we construct the first single-cell atlas of the human and porcine ocular compartments and study inter-species differences in the retina. In addition to that, we identify putative adult stem cells present in the iris tissue. We also create a disease map of genes involved in eye disorders across compartments of the eye. Furthermore, we probe the regulons of different cell populations, which include transcription factors and receptor-ligand interactions and reveal unique directional signalling between ocular cell types. In addition, we study conservation of regulons across vertebrates and zebrafish to identify common core factors. Here, we show perturbation of KLF7 gene expression during retinal ganglion cells differentiation and conclude that it plays a significant role in the maturation of retinal ganglion cells.


Assuntos
Diferenciação Celular/genética , Retina/metabolismo , Células Ganglionares da Retina/metabolismo , Análise de Célula Única/métodos , Transcriptoma/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Perfilação da Expressão Gênica/métodos , Humanos , Pessoa de Meia-Idade , Retina/citologia , Análise de Sequência de RNA/métodos , Especificidade da Espécie , Suínos
12.
Stem Cell Reports ; 16(2): 237-251, 2021 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-33450191

RESUMO

Recent trials of retinal pigment epithelium (RPE) transplantation for the treatment of disorders such as age-related macular degeneration have been promising. However, limitations of existing strategies include the uncertain survival of RPE cells delivered by cell suspension and the inherent risk of uncontrolled cell proliferation in the vitreous cavity. Human RPE stem cell-derived RPE (hRPESC-RPE) transplantation can rescue vision in a rat model of retinal dystrophy and survive in the rabbit retina for at least 1 month. The present study placed hRPESC-RPE monolayers under the macula of a non-human primate model for 3 months. The transplant was able to recover in vivo and maintained healthy photoreceptors. Importantly, there was no evidence that subretinally transplanted monolayers underwent an epithelial-mesenchymal transition. Neither gliosis in adjacent retina nor epiretinal membranes were observed. These findings suggest that hRPESC-RPE monolayers are safe and may be a useful source for RPE cell replacement therapy.


Assuntos
Xenoenxertos/transplante , Degeneração Macular/terapia , Epitélio Pigmentado da Retina/transplante , Transplante de Células-Tronco/métodos , Idoso , Idoso de 80 Anos ou mais , Animais , Proliferação de Células , Células Cultivadas , Modelos Animais de Doenças , Transição Epitelial-Mesenquimal , Feminino , Xenoenxertos/patologia , Humanos , Terapia de Imunossupressão , Macaca fascicularis , Masculino , Células Fotorreceptoras/fisiologia , Primatas , Retina/patologia , Retina/transplante , Epitélio Pigmentado da Retina/patologia
13.
SSRN ; : 3650574, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32742243

RESUMO

The outbreak of COVID-19 caused by the SARS-CoV-2 virus has created an unparalleled disruption of global behavior and a significant loss of human lives. To minimize SARS-CoV-2 spread, understanding the mechanisms of infection from all possible viral entry routes is essential. As aerosol transmission is thought to be the primary route of spread, we sought to investigate whether the eyes are potential entry portals for SARS-CoV-2. While virus has been detected in the eye, in order for this mucosal membrane to be a bone fide entry source SARS-CoV-2 would need the capacity to productively infect ocular surface cells.  As such, we conducted RNA sequencing in ocular cells isolated from adult human cadaver donor eyes as well as from a pluripotent stem cell-derived whole eye organoid model to evaluate the expression of ACE2 and TMPRSS2, essential proteins that mediate SARS-CoV-2 viral entry. We also infected eye organoids and adult human ocular cells with SARS-CoV-2 and evaluated virus replication and the host response to infection. We found the limbus was most susceptible to infection, whereas the central cornea exhibited only low levels of replication. Transcriptional profiling of the limbus upon SARS-CoV-2 infection, found that while type I or III interferons were not detected in the lung epithelium, a significant inflammatory response was mounted. Together these data suggest that the human eye can be directly infected by SARS-CoV-2 and thus is a route warranting protection. Funding: The National Eye Institute (NEI), Bethesda, MD, USA, extramural grant 1R21EY030215-01 and the Icahn School of Medicine at Mount Sinai supported this study.

14.
Stem Cell Reports ; 14(4): 631-647, 2020 04 14.
Artigo em Inglês | MEDLINE | ID: mdl-32243845

RESUMO

Epithelial to mesenchymal transition (EMT) is a biological process involved in tissue morphogenesis and disease that causes dramatic changes in cell morphology, migration, proliferation, and gene expression. The retinal pigment epithelium (RPE), which supports the neural retina, can undergo EMT, producing fibrous epiretinal membranes (ERMs) associated with vision-impairing clinical conditions, such as macular pucker and proliferative vitreoretinopathy (PVR). We found that co-treatment with TGF-ß and TNF-α (TNT) accelerates EMT in adult human RPE stem cell-derived RPE cell cultures. We captured the global epigenomic and transcriptional changes elicited by TNT treatment of RPE and identified putative active enhancers associated with actively transcribed genes, including a set of upregulated transcription factors that are candidate regulators. We found that the vitamin B derivative nicotinamide downregulates these key transcriptional changes, and inhibits and partially reverses RPE EMT, revealing potential therapeutic routes to benefit patients with ERM, macular pucker and PVR.


Assuntos
Epigenômica , Membrana Epirretiniana/prevenção & controle , Transição Epitelial-Mesenquimal , Modelos Biológicos , Niacinamida/uso terapêutico , Epitélio Pigmentado da Retina/patologia , Células-Tronco/metabolismo , Transcriptoma/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/metabolismo , Elementos Facilitadores Genéticos/genética , Membrana Epirretiniana/patologia , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Transição Epitelial-Mesenquimal/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Pessoa de Meia-Idade , Niacinamida/genética , Niacinamida/farmacologia , Fenótipo , Células-Tronco/efeitos dos fármacos , Fatores de Transcrição/metabolismo , Transcrição Gênica/efeitos dos fármacos , Fator de Crescimento Transformador beta1/farmacologia , Fator de Necrose Tumoral alfa/farmacologia
15.
Elife ; 82019 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-30624204

RESUMO

The rules governing cerebellar output are not fully understood, but must involve Purkinje cell (PC) activity, as PCs are the major input to deep cerebellar nuclear (DCN) cells (which form the majority of cerebellar output). Here, the influence of PC complex spikes (CSs) was investigated by simultaneously recording DCN activity with CSs from PC arrays in anesthetized rats. Crosscorrelograms were used to identify PCs that were presynaptic to recorded DCN cells (presynaptic PCs). Such PCs were located within rostrocaudal cortical strips and displayed synchronous CS activity. CS-associated modulation of DCN activity included a short-latency post-CS inhibition and long-latency excitations before and after the CS. The amplitudes of the post-CS responses correlated with the level of synchronization among presynaptic PCs. A temporal precision of ≤10 ms was generally required for CSs to be maximally effective. The results suggest that CS synchrony is a key control parameter of cerebellar output. Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).


Assuntos
Potenciais de Ação , Cerebelo/fisiologia , Células de Purkinje/fisiologia , Animais , Córtex Cerebral/fisiologia , Bases de Dados Factuais , Estimulação Elétrica , Feminino , Neurônios/fisiologia , Núcleo Olivar/fisiologia , Ratos , Ratos Sprague-Dawley , Sinapses/fisiologia
16.
Commun Biol ; 2: 162, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31069271

RESUMO

Proliferative vitreoretinopathy (PVR) is a metaplasia in the vitreous of the eye manifested by the transformation of retinal pigment epithelial (RPE) cells and the development of contracting epiretinal membranes (ERM), which lead to retinal detachment and vision loss. While TGFß1 and TNFα have been associated with PVR, here we show that these cytokines act synergistically to induce an aggressive contraction phenotype on adult human (ah)RPE. Connected RPE detach upon contraction and form motile membranes that recruit more cells. TGFß1 and TNFα (TNT)-induced contracting membranes uniquely express muscle and extracellular rearrangement genes. Whole transcriptome RNA sequencing of patient-dissected PVR membranes showed activation of the p38-MAPK signaling pathway. Inhibition of p38 during TNT treatment blocks ahRPE transformation and membrane contraction. Furthermore, TNT-induced membrane contractility can be reversed by p38 inhibition after induction. Therefore, targeting the p38-MAPK pathway may have therapeutic benefits for patients with PVR even after the onset of contracting ERMs.


Assuntos
Membrana Epirretiniana/genética , Descolamento Retiniano/genética , Fator de Crescimento Transformador beta1/genética , Fator de Necrose Tumoral alfa/genética , Vitreorretinopatia Proliferativa/genética , Proteínas Quinases p38 Ativadas por Mitógeno/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Movimento Celular , Membrana Epirretiniana/metabolismo , Membrana Epirretiniana/patologia , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Feminino , Regulação da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Cultura Primária de Células , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Descolamento Retiniano/metabolismo , Descolamento Retiniano/patologia , Epitélio Pigmentado da Retina/metabolismo , Epitélio Pigmentado da Retina/patologia , Transdução de Sinais , Imagem com Lapso de Tempo , Transcriptoma , Fator de Crescimento Transformador beta1/antagonistas & inibidores , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta1/farmacologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/metabolismo , Fator de Necrose Tumoral alfa/farmacologia , Vitreorretinopatia Proliferativa/metabolismo , Vitreorretinopatia Proliferativa/patologia , Corpo Vítreo/metabolismo , Corpo Vítreo/patologia , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
17.
Front Immunol ; 10: 708, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31118929

RESUMO

Ocular toxoplasmosis is the commonest clinical manifestation of infection with obligate intracellular parasite, Toxoplasma gondii. Active ocular toxoplasmosis is characterized by replication of T. gondii tachyzoites in the retina, with reactive inflammation. The multifunctional retinal pigment epithelium is a key target cell population for T. gondii. Since the global gene expression profile is germane to understanding molecular involvements of retinal pigment epithelial cells in ocular toxoplasmosis, we performed RNA-Sequencing (RNA-Seq) of human cells following infection with T. gondii tachyzoites. Primary cell isolates from eyes of cadaveric donors (n = 3), and the ARPE-19 human retinal pigment epithelial cell line, were infected for 24 h with GT-1 strain T. gondii tachyzoites (multiplicity of infection = 5) or incubated uninfected as control. Total and small RNA were extracted from cells and sequenced on the Illumina NextSeq 500 platform; results were aligned to the human hg19 reference sequence. Multidimensional scaling showed good separation between transcriptomes of infected and uninfected primary cell isolates, which were compared in edgeR software. This differential expression analysis revealed a sizeable response in the total RNA transcriptome-with significantly differentially expressed genes totaling 7,234 (28.9% of assigned transcripts)-but very limited changes in the small RNA transcriptome-totaling 30 (0.35% of assigned transcripts) and including 8 microRNA. Gene ontology and pathway enrichment analyses of differentially expressed total RNA in CAMERA software, identified a strong immunologic transcriptomic signature. We conducted RT-qPCR for 26 immune response-related protein-coding and long non-coding transcripts in epithelial cell isolates from different cadaveric donors (n = 3), extracted by a different isolation protocol but similarly infected with T. gondii, to confirm immunological activity of infected cells. For microRNA, increases in miR-146b and miR-212 were detected by RT-qPCR in 2 and 3 of these independent cell isolates. Biological network analysis in the InnateDB platform, including 735 annotated differentially expressed genes plus 2,046 first-order interactors, identified 10 contextural hubs and 5 subnetworks in the transcriptomic immune response of cells to T. gondii. Our observations provide a solid base for future studies of molecular and cellular interactions between T. gondii and the human retinal pigment epithelium to illuminate mechanisms of ocular toxoplasmosis.


Assuntos
Epitélio Pigmentado da Retina/imunologia , Epitélio Pigmentado da Retina/parasitologia , Toxoplasma/imunologia , Toxoplasma/patogenicidade , Toxoplasmose Ocular/genética , Toxoplasmose Ocular/imunologia , Idoso , Cadáver , Técnicas de Cultura de Células , Linhagem Celular , Separação Celular , Perfilação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Humanos , Fenômenos Imunogenéticos , MicroRNAs/genética , MicroRNAs/metabolismo , Pessoa de Meia-Idade , RNA-Seq , Epitélio Pigmentado da Retina/citologia , Toxoplasmose Ocular/parasitologia
18.
J Neurosci Methods ; 172(2): 312-22, 2008 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-18565591

RESUMO

Determining how a particular neuron, or population of neurons, encodes information in their spike trains is not a trivial problem, because multiple coding schemes exist and are not necessarily mutually exclusive. Coding schemes generally fall into one of two broad categories, which we refer to as rate and temporal coding. In rate coding schemes, information is encoded in the variations of the average firing rate of the spike train. In contrast, in temporal coding schemes, information is encoded in the specific timing of the individual spikes that comprise the train. Here, we describe a method for testing the presence of temporal encoding of information. Suppose that a set of original spike trains is given. First, surrogate spike trains are generated by randomizing each of the original spike trains subject to the following constraints: the local average firing rate is approximately preserved, while the overall average firing rate and the distribution of primary interspike intervals are perfectly preserved. These constraints ensure that any rate coding of information present in the original spike trains is preserved in the members of the surrogate population. The null-hypothesis is rejected when additional information is found to be present in the original spike trains, implying that temporal coding is present. The method is validated using artificial data, and then demonstrated using real neuronal data.


Assuntos
Potenciais de Ação/fisiologia , Eletrofisiologia/métodos , Neurônios/fisiologia , Neurofisiologia/métodos , Processamento de Sinais Assistido por Computador , Software/normas , Algoritmos , Animais , Interpretação Estatística de Dados , Gryllidae/fisiologia , Humanos , Modelos Neurológicos , Núcleo Olivar/fisiologia , Ratos , Células Receptoras Sensoriais/fisiologia , Validação de Programas de Computador , Fatores de Tempo
19.
Curr Protoc Stem Cell Biol ; 45(1): e53, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-30040247

RESUMO

An adult human retinal pigment epithelial layer (ahRPE) model derived from stem cells isolated from native RPE monolayers (ahRPE-SCs) exhibits key physiological characteristics of native tissue and therefore provides the means to create a human "disease in a dish" model to study RPE diseases. Traditionally, RPE lines are established from whole globes dedicated to research. Here we describe a new technique for establishing primary RPE lines from the posterior poles of globes used for corneal transplants. Since tissues from corneal transplants are derived from younger and healthier donors than those used for research, we have hypothesized that RPE cells isolated from corneal transplantation globes will result in improved primary RPE line establishment. Our new procedure increases the rate of establishing successful RPE cultures and improves the total cell number yield. Use of this advanced methodology can provide a new source of high-quality primary RPE line cultures. © 2018 by John Wiley & Sons, Inc.


Assuntos
Transplante de Córnea , Modelos Biológicos , Epitélio Pigmentado da Retina/citologia , Células-Tronco/citologia , Adulto , Proliferação de Células , Separação Celular , Impedância Elétrica , Células Epiteliais/citologia , Humanos , Fenótipo , Células-Tronco/metabolismo
20.
J Neurosci ; 26(6): 1739-48, 2006 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-16467522

RESUMO

Inferior olivary (IO) neurons are electrotonically coupled by gap junctions. This coupling is thought to underlie synchronous complex spike (CS) activity generated by the olivocerebellar system in Purkinje cells, and also has been hypothesized to be necessary for IO neurons to generate spontaneous oscillatory activity. These characteristics of olivocerebellar activity have been proposed to be central to the role of this system in motor coordination. However, the relationship of gap junction coupling between IO neurons to synchronous and rhythmic CS activity has never been directly tested. Thus, to address this issue, multiple electrode recordings were obtained from crus 2a Purkinje cells, and carbenoxolone, a gap junction blocker, was injected into the IO. Carbenoxolone reduced CS synchrony by 50% overall, but in some experiments, >80% reductions were achieved. Carbenoxolone also reduced the average firing rate by 50%, suggesting that electrical coupling is a significant source of excitation for IO neurons. Moreover, carbenoxolone caused a reduction in the approximately 10 Hz rhythmicity of CS activity, and this reduction was correlated with the extent to which the injection reduced CS synchrony. Lastly, carbenoxolone was found to reverse or prevent changes in synchrony that are normally induced by injection of GABAA and glutamate receptor antagonists into the IO, suggesting that the effects of these drugs on CS synchrony patterns require electrical coupling of IO neurons. In sum, our results provide direct evidence that electrical coupling of IO neurons underlies synchronous CS activity, and suggest important roles for this coupling in shaping other aspects of IO spiking patterns.


Assuntos
Junções Comunicantes/fisiologia , Neurônios/fisiologia , Núcleo Olivar/fisiologia , Células de Purkinje/fisiologia , Animais , Temperatura Corporal , Carbenoxolona/farmacologia , Córtex Cerebelar/fisiologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas GABAérgicos/farmacologia , Junções Comunicantes/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Células de Purkinje/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
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