RESUMO
We report the identification of a novel, high oxygen affinity hemoglobin (Hb) variant [α127(H10)LysâGln; HBA1: c.382A>C]. The variant was detected in an adolescent male (proband) of Syrian descent by cation exchange high performance liquid chromatography (HPLC), during Hb A1c analysis. A complete blood count (CBC) showed elevated red blood cells (RBCs) (6.08 × 1012/L), Hb (16.1 g/dL) and packed cell volume (PCV) (0.48 L/L). Capillary electrophoresis (CE) revealed the variant was more negatively charged and represented 18.2% of total Hb. Isopropanol stability was normal. Cyanosis in the subject prompted investigation of oxygen affinity, with a reduced p50 of 20.8 mm Hg and a left shifted oxygen dissociation curve demonstrating increased oxygen affinity. We propose the novel variant be named Hb Waikato, which reflects the Hospital Laboratory where the variant was discovered and region where the proband was born and herein describe characterization.
Assuntos
Hemoglobinas Anormais , Adolescente , Cromatografia Líquida de Alta Pressão , Hemoglobinas Glicadas/genética , Hemoglobinas Anormais/genética , Humanos , Masculino , Mutação , OxigênioRESUMO
Hb Tacoma [ß30(B12)ArgâSer] is a missense variant that is caused by either an AGG>AGT or AGG>AGC substitution at codon 30 of the HBB gene. Currently, the latter is classified as a rare cause of ß0-thalassemia (ß0-thal). We propose that HBB: c.93G>C has been incorrectly assigned as ß0-thal and discuss whether HBB: c.93G>T or HBB: c.93G>C should be classified as ß+-thal instead, or as ß-globin variants without thalassemic effect. We present several subjects who are heterozygous for Hb Tacoma, one with HBB: c.93G>T and two with HBB: c.93G>C, to support our conclusions.
Assuntos
Hemoglobinas Anormais , Talassemia beta , Hemoglobinas Anormais/genética , Humanos , Mutação de Sentido Incorreto , Globinas beta/genética , Talassemia beta/diagnóstico , Talassemia beta/genéticaRESUMO
We report the identification of a large deletion of the α-globin gene cluster, which removed both HBA2 and HBA1 and included the region from HBZ to HBQ1 on chromosome 16 (16p13.3). The α0-thalassemia (α0-thal) deletion was discovered in an Indian family residing in New Zealand. The proband was a 3-month-old female, who presented with a Hb H disease of unknown molecular origin. Routine hematology showed marked hypochromic microcytic anemia, with numerous Hb H inclusion bodies. In the absence of iron deficiency, there was a strong clinical suspicion of α-thal. On initial screening using a multiplex gap polymerase chain reaction (gap-PCR), only the common rightward deletion (-α3.7) was detected. Investigation of the proband's mother and father revealed the mother was heterozygous for the -α3.7 deletion, while none of the seven most common pathogenic α-thal deletions were detected in the father. Multiplex ligation-dependent probe amplification (MLPA) was employed to detect the presence of a novel α0-thal deletion in both the proband and her father. For the proband, the α0-thal deletion in combination with the -α3.7 deletion, eliminated three copies of HBA consistent with a clinical diagnosis of Hb H disease.
Assuntos
Deleção de Sequência , alfa-Globinas/genética , Talassemia alfa/diagnóstico , Talassemia alfa/genética , Adulto , Alelos , Eletroforese Capilar , Índices de Eritrócitos , Feminino , Testes Genéticos/métodos , Genótipo , Humanos , Índia , Lactente , Masculino , Reação em Cadeia da Polimerase Multiplex , Linhagem , Fenótipo , Talassemia alfa/sangueRESUMO
Urate is often viewed as an antioxidant. Here, we present an alternative perspective by showing that, when oxidized, urate propagates oxidative stress. Oxidation converts urate to the urate radical and the electrophilic products dehydrourate, 5-hydroxyisourate, and urate hydroperoxide, which eventually break down to allantoin. We investigated whether urate-derived electrophiles are intercepted by nucleophilic amino acid residues to form stable adducts on proteins. When urate was oxidized in the presence of various peptides and proteins, two adducts derived from urate (Mr 167 Da) were detected and had mass additions of 140 and 166 Da, occurring mainly on lysine residues and N-terminal amines. The adduct with a 140-Da mass addition was detected more frequently and was stable. Dehydrourate (Mr 166 Da) also formed transient adducts with cysteine residues. Urate-derived adducts were detected on human serum albumin in plasma of healthy donors. Basal adduct levels increased when neutrophils were added to plasma and stimulated, and relied on the NADPH oxidase, myeloperoxidase, hydrogen peroxide, and superoxide. Adducts of oxidized urate on serum albumin were elevated in plasma and synovial fluid from individuals with gout and rheumatoid arthritis. We propose that rather than acting as an antioxidant, urate's conversion to electrophiles contributes to oxidative stress. The addition of urate-derived electrophiles to nucleophilic amino acid residues, a process we call oxidative uratylation, will leave a footprint on proteins that could alter their function when critical sites are modified.
Assuntos
Ácido Úrico/química , Aminas/química , Sequência de Aminoácidos , Ativação Enzimática/efeitos dos fármacos , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/metabolismo , Humanos , Inflamação/metabolismo , Modelos Moleculares , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Conformação Proteica , Albumina Sérica/química , Albumina Sérica/metabolismo , Ácido Úrico/metabolismo , Ácido Úrico/farmacologiaRESUMO
Patients with hyperunstable α chain variants usually present with a thalassemic, rather than hemolytic, phenotype. Electrophoretic, ion exchange and reverse phase separations usually fail to detect the variant and when DNA sequencing identifies a 'silent' substitution it is usually presumed to be hyperunstable. We report the identification of such a variant, α32(B13)MetâIle; HBA1: c.99G>A, arising from a new mutation on the α1 gene. The hemoglobin (Hb) was unequivocally detected by the isopropanol stability test and confirmed as hyperunstable by mass spectrometry (MS) of the precipitate and lysate, which showed proportions of 55% and 2.5% of α chains, respectively. The instability appears to be driven by perturbation of globin-heme, and possibly α1ß1 subunit, interactions.
Assuntos
Hemoglobinas Anormais/química , Hemoglobinas Anormais/genética , Mutação , Adulto , Feminino , Humanos , Estabilidade ProteicaRESUMO
We report the identification of a novel, slightly unstable hemoglobin (Hb) variant [ß12(A9)Thr â Pro; HBB: c.37A > C] that came to our attention during Hb A1C ion exchange chromatography where it migrated as a trailing shoulder on the Hb A0 peak. On electrospray ionization mass spectrometry (ESI MS), this electrophoretically silent variant was detected as an unresolved ß component with a 2 Da decrease in average ß chain mass. Reversed phase high performance liquid chromatography (HPLC) confirmed the presence of a more hydrophilic ß chain with a mass 4 Da less than normal ß(A) and showed it represented 40.0% of the total ß-globin. Tryptic mapping revealed the [M + 1H] ion of peptide ßT-2 had shifted from 932.5 to 928.5 m/z, suggesting a point mutation of Thr â Pro at position ß12(A9). This substitution was confirmed by fragmentation analysis of the [M + 2H] ion (464.8 m/z) of the new ßT-2 peptide and it represents a novel mutation which we have named Hb Ashburton.
Assuntos
Códon/genética , Hemoglobinas Anormais/genética , Mutação de Sentido Incorreto , Globinas beta/genética , Idoso , Cromatografia Líquida de Alta Pressão , Hemoglobinas Anormais/química , Hemoglobinas Anormais/metabolismo , Humanos , Masculino , Oligopeptídeos , Prolina/genética , Espectrometria de Massas por Ionização por Electrospray , Treonina/genética , Globinas beta/química , Globinas beta/metabolismoRESUMO
We report a novel hemoglobin (Hb) mutation [Hb Papanui [α99(G8)LysâArg; HBA2: c.299A>G] that was identified in an individual with an inappropriately low Hb A1c value of 2.8% when using an ion exchange method. Although occurring at a well conserved site, the conservative substitution was not associated with any adverse clinical features.
Assuntos
Hemoglobinas Anormais/genética , Mutação , alfa-Globinas/genética , Adulto , Substituição de Aminoácidos , Códon , Feminino , Hemoglobinas Glicadas/química , Hemoglobinas Glicadas/genética , Hemoglobinas Anormais/química , HumanosAssuntos
Complexos Multiproteicos/sangue , Troponina I/sangue , Cromatografia em Gel , Angiografia por Tomografia Computadorizada , Vasos Coronários/diagnóstico por imagem , Ecocardiografia , Humanos , Masculino , Pessoa de Meia-Idade , Complexos Multiproteicos/isolamento & purificação , Infarto do Miocárdio/diagnóstico , Infarto do Miocárdio/diagnóstico por imagem , Placa Aterosclerótica/diagnóstico , Troponina I/isolamento & purificaçãoRESUMO
We report a novel α2 gene mutation identified in compound heterozygosity with the common -α(3.7) deletion. The mutation (c.349_359 del GAGTTCACCCC), causes a frameshift after codon α115 with the predicted extension of the αchain from 141 to 166 residues. The new polypeptide would be expected to have a mass of 17,463 Da, but no such product was detected in hemolysates, confirming the mutation's thalassemic phenotype. Mechanistically, the deletion is probably caused by replication slippage during DNA synthesis as the sequence is bracketed by a CC repeat. The lack of protein expression is probably caused by loss of critical binding sites to the chaperone, α Hb stabilizing protein (AHSP).
Assuntos
Códon/genética , Mutação da Fase de Leitura , Deleção de Sequência , alfa-Globinas/genética , Adulto , Sequência de Aminoácidos , Sequência de Bases , Análise Mutacional de DNA , Humanos , Masculino , Dados de Sequência Molecular , Talassemia/genéticaRESUMO
Mutations in the fibrinogen gene cluster can cause low plasma fibrinogen concentrations, known as hypofibrinogenaemia. It is important to verify whether a detected sequence variant in this cluster is deleterious or benign and this can be accomplished using protein expression systems. In this study, four mutations in the fibrinogen gammaC domain that had previously been described in patients with hypofibrinogenaemia were introduced into a gammaC construct and expressed in a Pichia pastoris yeast system to investigate their effects on protein stability and secretion. These experiments showed that the fibrinogen Middlemore (N230D), Dorfen (A289V), Mannheim II (H307Y), and Muncie (T371I) mutations were not secreted, supporting their causative role in hypofibrinogenaemia. Overexpression of the N230D, A289V and H307Y mutants revealed that the majority of the synthesised protein was retained in the endoplasmic reticulum, with only a minor proportion reaching the trans-Golgi network. Regardless, none of this protein was secreted which confirms that the four mutations investigated are indeed responsible for hypofibrinogenaemia.
Assuntos
Afibrinogenemia/genética , Fibrinogênio/genética , Fibrinogênio/metabolismo , Pichia/metabolismo , Fibrinogênio/química , Humanos , Mutagênese Sítio-Dirigida , Mutação , Pichia/genética , Estrutura Terciária de Proteína/genética , Transfecção , Rede trans-Golgi/genética , Rede trans-Golgi/metabolismoRESUMO
Mutation in fibrinogen genes may lead to quantitative or qualitative disorders that result in bleeding, thrombosis or hepatic fibrinogen storage disease. Only three mutations in the fibrinogen γ gene have been identified that cause hepatic endoplasmic reticulum storage of mutant fibrinogen. To investigate the possibility of hepatic fibrinogen storage disease in a 4-year-old male with persistently elevated serum aminotransferases and preserved synthetic function except for a prolonged INR. After informed consent, liver and blood samples were obtained. Liver sections were examined by light microscopy, anti-fibrinogen immunolabelling and electron microscopy. Purified fibrinogen was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse phase high performance liquid chromatography; DNA sequencing was performed using a BigDye Terminator (v. 3.1) cycle sequencing kit. Four-year-old male with persistently elevated transaminases with an INR 1.5 but otherwise normal synthetic function. Fibrinogen activity and thrombin clotting time were abnormal at 0.47 g/L and 46 s respectively. Hepatic histological examination revealed portal inflammatory infiltrates with bridging fibrosis. Clumped eosinophilic material was observed in hepatocytes that was immunoreactive to fibrinogen antisera. Ultrastructural examination showed cytoplasmic inclusions arrayed in fingerprint-like patterns. DNA sequence analysis revealed heterozygosity for a novel γ314Thr âPro mutation (fibrinogen AI duPont) in the fibrinogen γ gene. Protein analyses showed normal patterns of Aα, Bß and γ chains suggesting that the variant γ allele was not expressed in plasma fibrinogen. We describe only the fourth mutation to be identified, γ314ThrâPro (fibrinogen AI duPont), giving rise to hypofibrinogenaemia and hepatic fibrinogen storage disease.
Assuntos
Afibrinogenemia/genética , Fibrinogênio/genética , Hepatopatias/genética , Fígado/metabolismo , Erros Inatos do Metabolismo/genética , Mutação , Afibrinogenemia/metabolismo , Afibrinogenemia/patologia , Alanina Transaminase/sangue , Aspartato Aminotransferases/sangue , Biomarcadores/sangue , Coagulação Sanguínea , Pré-Escolar , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Análise Mutacional de DNA , Eletroforese em Gel de Poliacrilamida , Fibrinogênio/química , Fibrinogênio/metabolismo , Predisposição Genética para Doença , Humanos , Corpos de Inclusão/metabolismo , Corpos de Inclusão/ultraestrutura , Coeficiente Internacional Normatizado , Fígado/ultraestrutura , Hepatopatias/metabolismo , Hepatopatias/patologia , Masculino , Erros Inatos do Metabolismo/metabolismo , Erros Inatos do Metabolismo/patologia , Modelos Moleculares , Fenótipo , Conformação Proteica , Relação Estrutura-AtividadeRESUMO
A second case of Hb Perpignan [beta136(H14)Gly-->Ser] was identified in a Burmese woman living in New Zealand. Although previously detected in France, this is the first formal description of the variant, which is electrophoretically silent and hematologically normal. This presentation as a benign substitution is in keeping with the low level of phylogenetic conservation of the H14 glycine.
Assuntos
Substituição de Aminoácidos , Hemoglobinas Anormais/química , Mutação de Sentido Incorreto , Mutação Puntual , Globinas beta/genética , Adulto , Eletroforese das Proteínas Sanguíneas , Feminino , Hemoglobinas Anormais/genética , Humanos , Mianmar/etnologia , Nova Zelândia , Mapeamento de Peptídeos , Estabilidade Proteica , Espectrometria de Massas por Ionização por Electrospray , Globinas beta/químicaRESUMO
Hb Constant Spring [(Hb CS) alpha142, Term-->Gln (TAA>CAA in alpha2)] and Hb Koya Dora [alpha142, Term-->Ser (TAA>TCA in alpha2)] both involve mutations of the alpha2 gene stop codon and while Hb CS is the most frequent cause of nondeletional alpha-thalassemia (alpha-thal) in Southeast Asia, Hb Koya Dora is limited to a restricted population from Andhra Pradesh, India. Here we identify a homozygous case of Hb Koya Dora and confirm the structure of the 31 residue alpha chain extension.
Assuntos
Códon de Terminação/genética , Hemoglobinas Anormais/genética , Mutação , Talassemia alfa/genética , Adulto , Cromatografia Líquida de Alta Pressão , Feminino , Predisposição Genética para Doença/genética , Hemoglobinas Anormais/análise , Homozigoto , Humanos , Espectrometria de Massas por Ionização por Electrospray , Talassemia alfa/metabolismoRESUMO
Albumin has an average plasma half-life of three weeks and is thus an attractive carrier to improve the pharmacokinetics of fused therapeutics. The half-life is regulated by FcRn, a cellular receptor that protects against intracellular degradation. To tailor-design the therapeutic use of albumin, it is crucial to understand how structural alterations in albumin affect FcRn binding and transport properties. In the blood, the last C-terminal residue (L585) of albumin may be enzymatically cleaved. Here we demonstrate that removal of the L585 residue causes structural stabilization in regions of the principal FcRn binding domain and reduces receptor binding. In line with this, a short half-life of only 3.5 days was measured for cleaved albumin lacking L585 in a patient with acute pancreatitis. Thus, we reveal the structural requirement of an intact C-terminal end of albumin for a long plasma half-life, which has implications for design of albumin-based therapeutics.
Assuntos
Albumina Sérica Humana/metabolismo , Amilases/sangue , Animais , Carboxipeptidases A/sangue , Meia-Vida , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe I/metabolismo , Humanos , Lipase/sangue , Masculino , Camundongos Transgênicos , Pâncreas/enzimologia , Pancreatite/sangue , Pancreatite/enzimologia , Ligação Proteica , Domínios Proteicos , Estabilidade Proteica , Proteólise , Receptores Fc/genética , Receptores Fc/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Albumina Sérica Humana/química , Albumina Sérica Humana/genética , Relação Estrutura-AtividadeRESUMO
We previously described a novel homozygous point mutation (FGB c.115-600A>G) located deep within intron 1 of the fibrinogen beta gene (FGB), as a likely cause of afibrinogenemia. While this was the only mutation detected, its pathological mechanism was unclear. Here we show the mutation causes the inclusion of a 50-bp cryptic exon by creating a consensus heptad motif recognized by the spliceosome recruiting protein pre-mRNA splicing factor (SF2)/arginine/serine-rich alternative splicing factor (ASF) splicing factor 2/alternative splicing factor (SF2/ASF). Translation of the aberrant mRNA would result in truncation of the Bbeta chain, preventing fibrinogen synthesis. Selective introduction of a second mutation into the enhancer motif abolished the SF2/ASF binding motif and re-established normal pre-mRNA splicing. Subsequent introduction of antisense phosphorodiamidate morpholino oligonucleotides (PMOs) into transfected cells containing the mutant construct blocked the protein-RNA interaction and successfully restored normal splicing ( approximately 50% at 2 microM and approximately 90% at 10 microM). The molecular characterization of this case has revealed a unique disease mechanism, shown the importance of screening for deep intronic mutations, and provided evidence that antisense gene therapy is potentially practical for the treatment of diseases caused by this class of mutation.
Assuntos
Afibrinogenemia/genética , Elementos Facilitadores Genéticos , Éxons/genética , Fibrinogênio/genética , Íntrons/genética , Mutação/genética , Splicing de RNA/genética , Animais , Sequência de Bases , Células COS , Chlorocebus aethiops , Sequência Consenso , Humanos , Dados de Sequência Molecular , Oligonucleotídeos Antissenso/farmacologia , Splicing de RNA/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
A 30-year-old female experienced three miscarriages in early pregnancy. Extensive laboratory screening showed a low plasma fibrinogen level of approximately l g/l detected by PT-derived fibrinogen assay. The fibrinogen level in the immunological assay was 3 g/l. The functional Clauss assay yielded an intermediate result of 1.78 g/l. During her fourth and fifth pregnancy, the patient received fibrinogen concentrates (Haemocomplettan, CLS Behring, Marburg, Germany), starting with 4 grams of human fibrinogen, followed by 2 grams every second day until the 15(th) week of pregnancy. The further course of these pregnancies was uneventful. SDS-PAGE and immunoblotting showed doublet bands in the positions of the high-molecular weight (HMW)- and low-molecular-weight (LMW)-fibrinogen, a single LMW' fibrinogen band, plus additional bands with higher molecular weight than HMW-fibrinogen, which were also reactive with anti-human serum albumin (HSA) antiserum. These bands correspond to variant fibrinogen conjugated with albumin. Reduced SDS-PAGE and immunoblotting using polyclonal anti-fibrinopeptide A antiserum disclosed one additional Aalpha-chain band with lower molecular weight. Amplification and sequencing of exon 5 of the alpha gene indicated heterozygosity for a novel single nucleotide deletion at codon Aalpha494 (C1537delA). His494 is replaced by Pro and this is followed by 23 (LMKLPSSTLPQLEKHSQ VSSHLC) new amino acids before premature truncation after Cys517, yielding a free C-terminal cysteine, which may link with albumin. This new fibrinogen mutation, leads to a balanced array of homo- and heterodimeric fibrinogen molecules, some of which are conjugated to albumin.
Assuntos
Aborto Habitual/sangue , Aborto Habitual/genética , Fibrinogênios Anormais/genética , Albumina Sérica/metabolismo , Adulto , Sequência de Aminoácidos , Dimerização , Feminino , Fibrinogênios Anormais/química , Fibrinogênios Anormais/metabolismo , Variação Genética , Humanos , Dados de Sequência Molecular , Peso Molecular , Mutação Puntual , GravidezRESUMO
Routine pre-surgical coagulation investigations led to the detection of a novel type of hypodysfibrinogenemia whose functional defect appears to result from an alteration in the spacing between the functional domains of the fibrinogen molecule. The detection, by reverse phase HPLC, of a minor isoform of Bbeta chain with a 554 Da decrease in mass led to the identification of a deletion of five amino acids (NVVNE) from the center of the coiled coil. The variant chain contributed only 10% of the total Bbeta material and the mutation (BbetaAsn167_Glu171del) was associated with both increased clotting times and low functional and physical fibrinogen concentrations in 3 family members. There was a significant history of pregnancy-associated bleeding and miscarriage within the first trimester. Mechanistically the 15-nucleotide deletion appears to arise from replication advancement during DNA synthesis caused by a flanking pentanucleotide repeat of AATGA.
Assuntos
Afibrinogenemia/genética , Fibrinogênios Anormais/genética , Deleção de Sequência , Aborto Espontâneo/genética , Saúde da Família , Feminino , Fibrinogênios Anormais/química , Hemorragia , Humanos , Masculino , Hemorragia Pós-Parto/genética , Adulto JovemRESUMO
The identification of a second case of Hb Fontainbleau [alpha21(B2)Ala-->Pro] allowed us to re-examine its association with microcytosis, explore the effects of the mutation on protein stability and define the mutation at a DNA level. Although slightly unstable, the variant was expressed at 28-29% of the total and was caused by a heterozygous mutation in the alpha2 gene. There was no evidence for concomitant alpha-thalassemia (alpha-thal); both alpha-globin gene deletion analysis and sequencing of the alpha-globin locus failed to detect any additional mutations that might explain the relatively high expression level.
Assuntos
Anemia/sangue , Anemia/genética , Hemoglobinas Anormais/genética , Adulto , Alanina/genética , Substituição de Aminoácidos , Índices de Eritrócitos , Hemoglobinas Anormais/análise , Humanos , Masculino , Prolina/genética , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Genomic rearrangements are a well-recognized cause of genetic disease and can be formed by a variety of mechanisms. We report a complex rearrangement causing severe hemophilia A, identified and further characterized using a range of PCR-based methods, and confirmed using array-comparative genomic hybridization (array-CGH). This rearrangement consists of a 15.5-kb deletion/16-bp insertion located 0.6 kb from a 28.1-kb deletion/263-kb insertion at Xq28 and is one of the most complex rearrangements described at a DNA sequence level. We propose that the rearrangement was generated by distinct but linked cellular responses to double strand breakage, namely break-induced replication (BIR) and a novel model of break-induced serial replication slippage (SRS). The copy number of several genes is affected by this rearrangement, with deletion of part of the Factor VIII gene (F8, causing hemophilia A) and the FUNDC2 gene, and duplication of the TMEM185A, HSFX1, MAGEA9, and MAGEA11 genes. As the patient exhibits no clinically detectable phenotype other than hemophilia A, it appears that the biological effects of the other genes involved are not dosage-dependent. This investigation has provided novel insights into processes of DNA repair including BIR and the first description of SRS during repair in a pathological context.
Assuntos
Cromossomos Humanos X/genética , Fator VIII/genética , Mutação , Adolescente , Antígenos de Neoplasias/genética , Sequência de Bases , Análise Mutacional de DNA , Proteínas de Ligação a DNA/genética , Duplicação Gênica , Fatores de Transcrição de Choque Térmico , Proteínas de Choque Térmico/genética , Hemofilia A/genética , Hemofilia A/patologia , Humanos , Masculino , Modelos Genéticos , Mutagênese Insercional , Proteínas de Neoplasias/genética , Proteínas do Tecido Nervoso/genética , Receptores Acoplados a Proteínas G/genética , Deleção de SequênciaRESUMO
In a previous paper, we reported more efficient enterokinase cleavage at a C-terminal non-target LKGDR(201) site compared with an internally sited canonical recognition site, DDDDK(156). When this non-target site was placed internally to replace DDDDK(156) between the thioredoxin moiety and mouse NT-proCNP(1-50), this site was poorly processed leading us to conclude that efficient processing at LKGDR(201) in the first instance was due to its accessibility at the C-terminus of the fusion protein. Subsequently, we reasoned that treatment of thioredoxin-fused NT-proCNP(1-81) would allow us to retrieve full-length NT-proCNP(1-81) without undue processing at the LKGDR(201) site since this non-target site would now be located internally about 36 residues away from the C-terminus and hence not be hydrolyzed efficiently. Surprisingly, ESI-MS data showed that the LKGDR site in thioredoxin-fused human NT-proCNP(1-81) was still very efficiently cleaved and revealed a new but slow hydrolysis site with the sequence RVDTK/SRAAW to yield a peptide consistent with NT-proCNP(58-81). The evidence obtained from these experiments led us to postulate that efficient cleavage at the non-target LKGDR(201) site was not merely influenced by steric constraints but also by the sequence context downstream of the scissile bond. Hence, we constructed variants of thioredoxin-mouse NT-proCNP(1-50) where SRLLR residues (i.e. those immediately downstream from the LKGDR(201) site in NT-proCNP(1-50)) were systematically added one at a time downstream of the internal DDDDK(156) site. To evaluate the relative effects of site accessibility and downstream sequence context on the efficiency of enterokinase cleavage, we have also replaced the native LKGDR(201) sequence with DDDDK(201). Our results showed that incremental addition of SRLLR residues led to a steady increase in the rate of hydrolysis at DDDDK(156). Further variants comprising DDDDK(156)SS, DDDDK(156)SD and DDDDK(156)RR showed that the minimal critical determinants for enhanced enterokinase cleavage are serine in the P1' position followed by a serine or a basic residue, lysine or arginine, in the P2' position. Our data provided conclusive evidence that the influence of downstream sequences on recombinant light chain enterokinase activity was greater than accessibility of the target site at the terminus region of the protein. We further showed that the catalytic efficiency of the native holoenzyme was influenced primarily by residues on the N-terminal side of the scissile bond while being neutral to residues on the C-terminal side. Finally, we found that cleavage of all nine fusion proteins reflects accurate hydrolysis at the DDDDK(156) and DDDDK(201) sites when recombinant light chain enterokinase was used while non-specific processing at secondary sites were observed when these fusion proteins were treated with the native holoenzyme.