RESUMO
Nematodes include both free-living species such as Caenorhabditis elegans and major parasites of humans, livestock and plants. The apparent simplicity and uniformity of their nervous system belies a rich diversity of putative signalling molecules, particularly neuropeptides. This new appreciation stems largely from the genome-sequencing project with C. elegans, which is due to be completed by the end of 1998. The project has provided additional insights into other aspects of nematode neurobiology, as have studies on the mechanism of action of anthelmintics. Here, progress on the identification, localization, synthesis and physiological actions of transmitters identified in nematodes is explored.
Assuntos
Nematoides/metabolismo , Neurotransmissores/genética , Neurotransmissores/metabolismo , Sequência de Aminoácidos/genética , Animais , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Dados de Sequência Molecular , Distribuição Tecidual/fisiologiaRESUMO
1. Commonly used anthelmintic agents act on the muscle cells of parasitic nematodes to cause paralysis of the parasite and its expulsion from the host. 2. The motonervous system of nematodes contains neuropeptides, many of which are myoactive and elicit prolonged worm paralysis. Here we describe the actions of a novel peptide, KPNFIRFamide (Lys-Pro-Asn-Phe-Ileu-Arg-Phe-amide; PF4), which mediates relaxation of the somatic muscle of the parasitic nematode Ascaris suum. Its mechanism of action is compared to that of the inhibitory neuromuscular junction transmitter, gamma-aminobutyric acid (GABA), which gates a chloride channel on Ascaris muscle. 3. Both PF4 and GABA hyperpolarized the muscle cells (EC50 values 98 nM and 59 microM, respectively; n = 6) and this was accompanied by an increase in input conductance. 4. The increase in input conductance elicited by PF4 and a supramaximal concentration of GABA were additive (10 microM PF4, 7.78 +/- 1.88 microS; 10 mM GABA, 4.68 +/- 1.39 microS; 10 mM GABA and 10 microM PF4 12.05 +/- 2.6 microS, n = 6, P < 0.02 with respect to PF4 alone; P < 0.01 with respect to GABA alone). 5. The membrane potential response to 10 microM PF4 initially consisted of a fast hyperpolarization that occurred within 1 min of PF4 application. The reversal potential for this early response to PF4 (PF4-early) was determined at different extracellular chloride concentrations. Linear regression analysis of the natural logarithm of the extracellular chloride concentration against the reversal potential for PF4-early yielded a straight line with a slope of -29.6 +/- 2.4 (-34.4 to -24.9, 95% confidence limits; r2 = 0.82). This is close to the slope of -26.5 for a chloride-dependent event, as predicted by the Nernst equation. There was a significant correlation between the reversal potential for this event and the reversal potential for GABA (r = 0.94; P < 0.001; n = 12). 6. The late response to PF4 (PF4-late) appeared after 1 min and consisted of a slow reduction in the hyperpolarization to a plateau level, before the return of the membrane potential to the resting value. PF4-late is not likely to be a chloride-dependent event as during the hyperpolarization caused by a supramaximal concentration of GABA the muscle cells depolarized when a supramaximal concentration of PF4 was added to the perfusate. The membrane potential in the presence of 1 mM GABA was -61.8 +/- 4.8 mV and in the presence of 1 mM GABA with 10 microM PF4 was -47.5 +/- 1.5 mV (P < 0.02; n = 6). 7. The conductance increase elicited by 30 microM GABA was blocked by 10 microM ivermectin (before ivermectin 0.97 +/- 0.2 microS, after ivermectin 0.33 +/- 0.12 microS; n = 5; P < 0.05; Student's paired t test) but the conductance increase elicited by 1 microM PF4 was not (before ivermectin 0.96 +/- 0.14 microS, after ivermectin 1.07 +/- 0.19 microS; n = 0.34; Student's paired t test). 8. These data indicate that PF4 elicits a potent, inhibition of Ascaris muscle cells which is partially mediated by chloride and which is independent of the inhibitory GABA receptor.
Assuntos
Ascaris suum/fisiologia , Músculos/citologia , Neuropeptídeos/farmacologia , Oligopeptídeos/farmacologia , Animais , Antinematódeos/farmacologia , Eletrofisiologia , Técnicas In Vitro , Ivermectina/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Neurônios Motores/efeitos dos fármacos , Músculos/inervação , Músculos/metabolismo , Condução Nervosa/efeitos dos fármacos , Estimulação Química , Ácido gama-Aminobutírico/metabolismo , Ácido gama-Aminobutírico/farmacologiaRESUMO
The endogenous nematode peptides known as FMRFamide-related peptides (FaRPs) and various "classical" transmitters have a range of effects on nematodes that result in changes in behavior, particularly locomotion, including paralysis and inhibition of feeding. This study describes the application of an in vitro pharmacological approach to further delineate the action of a number of FaRP neurotransmitters on feeding behavior. Contraction of Ascaris suum pharyngeal muscle was monitored using a modified pressure transducer system that detects changes in intrapharyngeal pressure and therefore contraction of the radial muscle of the pharynx. The pharynx did not contract spontaneously. However, serotonin (5-HT, 100 microM) stimulated rhythmic contractions and relaxations (pumping) at a frequency of 0.5 Hz. The native nematode peptide, KNEFIRFamide (AF1), inhibited the pumping elicited by 5-HT. The duration of inhibition was concentration-dependent (1-1000 nM) with a threshold of 1 nM (n = 7). KSAYMRFamide (AF8/PF3) also inhibited pharyngeal pumping. There was no observable effect of any of the following nematode peptides on pharyngeal pumping behavior (1-1000 nM; n = 8): AF2, AF3, AF4, AF6, AF16, PF1/CF1, PF2/CF2, or PF4. Thus, interruption of pharyngeal processes, such as feeding, regulation of hydrostatic pressure, and secretion, may provide a new site of anthelmintic action.
Assuntos
Ascaris suum/fisiologia , FMRFamida/análogos & derivados , FMRFamida/farmacologia , Músculos/fisiologia , Faringe/fisiologia , Sequência de Aminoácidos , Animais , Músculos/efeitos dos fármacos , Faringe/efeitos dos fármacos , Relação Estrutura-AtividadeRESUMO
The pharyngeal muscle of nematodes consists of a syncytium of radial muscle whereby feeding occurs by the process of pharyngeal pumping. It is believed that the pumping behaviour of the pharynx may be partly controlled by the enteric nervous system (ENS), a component of the nematode nervous system which is associated predominantly with the pharynx. The distribution of serotoninergic and peptidergic (especially SALMF-amide-like) immunostaining is widespread in the ENS of Ascaris, being localized within the lateral and dorsal pharyngeal nerve tracts, the pharyngeal commissures, the nerve plexuses and associated nerve cells and fibres. Immunostaining for serotonin (5-HT) was only localized within the ENS. This paper also describes a method to enable in vitro pharmacological studies on the Ascaris pharynx. The effects of "classical" neurotransmitters and native nematode peptides on pharyngeal pumping behaviour in Ascaris have been investigated. The function of the pharynx in Ascaris is discussed.
Assuntos
Ascaris suum/anatomia & histologia , Ascaris suum/fisiologia , Faringe/anatomia & histologia , Faringe/fisiologia , Sequência de Aminoácidos , Animais , Sistema Nervoso Entérico/anatomia & histologia , Sistema Nervoso Entérico/efeitos dos fármacos , Sistema Nervoso Entérico/fisiologia , Imuno-Histoquímica , Hormônios de Invertebrado/química , Hormônios de Invertebrado/farmacologia , Neuropeptídeos/química , Neuropeptídeos/farmacologia , Neurotransmissores/farmacologia , Músculos Faríngeos/anatomia & histologia , Músculos Faríngeos/efeitos dos fármacos , Músculos Faríngeos/fisiologia , Faringe/inervação , Serotonina/metabolismo , Serotonina/farmacologiaRESUMO
The localization and distribution of SALMF amide (S1)-immunoreactivity (IR) in the nervous system of both the trematode, Diclidophora merlangi, and the cestode, Grillotia erinaceus, have been determined by an indirect immunofluorescent technique. Immunostaining was widespread in the central (CNS) and peripheral (PNS) nervous systems of both species. Antigen pre-absorption studies indicated that the results obtained do suggest S1-like immunostaining and not cross-reactivity with other peptides, in particular, neuropeptide F (NPF) and FMRF amide.
Assuntos
Cestoides/metabolismo , Sistema Nervoso/metabolismo , Neuropeptídeos/metabolismo , Trematódeos/metabolismo , Animais , Cestoides/anatomia & histologia , Técnica Indireta de Fluorescência para Anticorpo , Imuno-Histoquímica , Trematódeos/anatomia & histologiaRESUMO
The localisation and distribution of FMRF amide and neuropeptide F (NPF) immunoreactivities in the nervous system of adult male and female Schistosome mansoni have been determined by an indirect immunofluorescent technique in conjunction with confocal scanning laser microscopy (CSLM). The patterns of immunostaining were similar for the two peptides. In the central nervous system (CNS) immunoreactivity was evident in nerve cells and fibres in the anterior ganglia, central commissure and three pairs of nerve cords. In the peripheral nervous system (PNS), immunoreactivity was present in nerve plexuses associated with the pharynx, oral and ventral suckers, the subtegumental musculature and with both the gynaecophoric canal and in nerve fibres innervating the dorsal tubercles of the male. In the reproductive system of the male and female worms, immunostaining was only observed around the ootype/Mehlis' gland complex in the female. A post-embedding, IgG-conjugated colloidal gold immunostaining technique was employed to examine the subcellular distribution of FMRF amide immunoreactivity in the CNS of S. mansoni. Gold labelling of peptide was localised over dense-cored vesicles within nerve processes constituting the neuropile of the anterior ganglia. Blocking studies with homologous and heterologous peptides indicate that the immunostaining patterns are due to two distinct peptide molecules.
Assuntos
Neuropeptídeos/metabolismo , Schistosoma mansoni/metabolismo , Animais , FMRFamida , Feminino , Proteínas de Helminto/metabolismo , Humanos , Imuno-Histoquímica , Hormônios de Invertebrado/metabolismo , Masculino , Camundongos , Microscopia Confocal , Microscopia Imunoeletrônica , Sistema Nervoso/metabolismo , Sistema Nervoso/ultraestrutura , Neurotransmissores/metabolismo , Schistosoma mansoni/ultraestruturaRESUMO
The localization and distribution of glutamate-like immunoreactivity (IR) in the nervous system of both the cestode Mesocestoides corti and the trematode Fasciola hepatica has been determined by an indirect immunofluorescent technique, in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was widespread in the central (CNS) and peripheral (PNS) nervous systems of both species examined. In the CNS, IR was evident in nerve cells and fibres in the cerebral ganglia, the cerebral commissure and the dorsal, ventral and longitudinal nerve cords. In the peripheral nervous system (PNS) of M. corti, IR was apparent in nerve plexuses associated with the subtegmental musculature and the musculature associated with the anteriorly positioned suckers. In F. hepatica, IR was evident in the innervation of both the oral and the ventral suckers. In the reproductive system of F. hepatica, glutamate-IR was observed around the ootype/Mehlis' gland complex.
Assuntos
Sistema Nervoso Central/metabolismo , Fasciola hepatica/metabolismo , Ácido Glutâmico/metabolismo , Mesocestoides/metabolismo , Sistema Nervoso Periférico/metabolismo , Animais , Fasciola hepatica/ultraestrutura , Técnica Indireta de Fluorescência para Anticorpo , Mesocestoides/ultraestrutura , Microscopia Confocal , Fibras Nervosas/metabolismo , Neurônios/metabolismoRESUMO
The localisation and distribution of neuropeptides in the peripheral nervous system of the pig roundworm Ascaris suum have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Of the 31 antisera tested, immunostaining was obtained only with antisera to peptide YY (PYY), pancreatic polypeptide (PP) and FMRFamide. Immunostaining for PYY and FMRFamide was evident in the amphidial and papillary ganglia associated with the anterior nerve ring and in the nerves from these ganglia that terminated in sensory receptors within the buccal lips of the parasite. The only peptide immunoreactivity (IR) observed in the reproductive system of either sex was that evident in the nerve supply to the distal region of the vagina in the female worm. It took the form of a well-developed plexus of parallel nerve fibres, cross-connectives and looped commissures. The nerve net diminished in the more proximal region of the vagina. PP-IR was less intense than that for PYY and FMRFamide and was more restricted in distribution, being confined to a small number of nerve fibres in the nerve supply to the vagina; it did not occur in the nerves supplying the anterior sensory receptors. The possible roles of neuropeptides in the sensory and reproductive biology of nematodes are discussed.
Assuntos
Ascaris suum/química , Neuropeptídeos/isolamento & purificação , Nervos Periféricos/química , Células Receptoras Sensoriais/química , Animais , FMRFamida , Feminino , Imunofluorescência , Genitália Feminina/química , Imuno-Histoquímica , Masculino , Microscopia Eletrônica de Varredura , Neuropeptídeo Y/isolamento & purificação , SuínosRESUMO
The anthelmintic invermectin has a number of effects on nematodes which result in changes in behaviour, particularly locomotion, including paralysis and an inhibition of feeding. This paper describes the application of an in vitro pharmacological approach to further delineate the action of ivermectin on feeding behaviour. Contraction of Ascaris suum pharyngeal muscle was monitored using a modified pressure transducer system which detects changes in intrapharyngeal pressure and therefore contraction of the radial muscle of the pharynx. The pharynx did not contract spontaneously. However, serotonin (5-HT, 100 microM) stimulated rhythmic contractions and relaxations (pumping) at a frequency of 0.5 Hz. gamma-Aminobutyric acid (GABA) and glutamic acid inhibited the pumping elicited by 5-HT. The duration of inhibition was concentration dependent (1-1000 microM) with a threshold of 1 microM and 10 microM respectively (n = 8). Ivermectin also inhibited pharyngeal pumping (1-1000 nM). At lower concentrations, ivermectin (1-10 pM) potentiated the GABA and glutamate inhibition, so that inhibition occurred at concentrations which were below threshold in the absence of ivermectin. These data provide evidence that the pharynx is a site for the action of ivermectin. Thus interruption of pharyngeal processes such as, feeding, regulation of hydrostatic pressure and secretion may provide a new site of anthelmintic action.
Assuntos
Anti-Helmínticos/farmacologia , Ascaris suum/efeitos dos fármacos , Comportamento Alimentar/efeitos dos fármacos , Ivermectina/farmacologia , Músculos Faríngeos/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Interações Medicamentosas , Sistema Nervoso Entérico/efeitos dos fármacos , Ácido Glutâmico/farmacologia , Contração Muscular/efeitos dos fármacos , Ácido gama-Aminobutírico/farmacologiaRESUMO
A post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (PP) and to the invertebrate peptide, FMRFamide within the central nervous system (CNS) of the nematode, Ascaris suum. Gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the CNS. Double-labelling of peptides demonstrated an apparent co-localization of PP and FMRFamide immunoreactivities in the same dense-cored vesicles, although populations of dense-cored vesicles that labelled solely for FMRFamide were also evident. Antigen preabsorption studies indicated little or no cross-reactivity between the two antisera.
Assuntos
Ascaris suum/metabolismo , Neuropeptídeos/metabolismo , Polipeptídeo Pancreático/metabolismo , Animais , Antígenos de Helmintos/metabolismo , Ascaris suum/imunologia , Ascaris suum/ultraestrutura , Sistema Nervoso Central/metabolismo , Reações Cruzadas , FMRFamida , Imuno-Histoquímica , Microscopia Imunoeletrônica , Neurônios/imunologia , Neurônios/metabolismo , Neurônios/ultraestrutura , Neuropeptídeos/imunologia , Polipeptídeo Pancreático/imunologiaRESUMO
The localization and distribution of neuropeptides and an indoleamine (serotonin or 5-hydroxytryptamine) in the enteric nervous system (ENS) of the pig roundworm, Ascaris suum, have been determined by the application of an indirect immunofluorescence technique in conjunction with confocal scanning laser microscopy. Whole-mount preparations of pharyngeal, intestinal and rectal regions were screened with antisera to 23 vertebrate peptides, 2 invertebrate peptides and serotonin (= 5-HT). Positive immunoreactivity (IR) was obtained with antisera to pancreatic polypeptide (PP), peptide YY (PYY), FMRFamide, gastrin and serotonin. The only IR observed in the ENS was that evident in the nerve supply to the pharynx and rectal region; no IR was associated with any region of the intestine. The most extensive patterns of IR occurred with antisera to PYY, FMRFamide and serotonin. In the pharyngeal component of the ENS, IR was evident in the lateral and dorsal longitudinal pharyngeal nerves, pharyngeal commissures, nerve plexus, and associated nerve cells and fibres. In contrast, the distribution of IR to the PP and gastrin antisera was more restricted and displayed a lower intensity of immunostaining. The other component of the ENS, the rectal enteric system, only yielded immunostaining to FMRFamide. The possible role of neuropeptides and serotonin in the nutritional biology of nematodes is discussed.
Assuntos
Ascaris suum/química , Neuropeptídeo Y/análise , Neuropeptídeos/análise , Neurotransmissores/análise , Serotonina/análise , Animais , Sistema Nervoso Entérico/química , FMRFamida , Gastrinas/análise , Imuno-Histoquímica , Hormônios de Invertebrado/análise , SuínosRESUMO
The localization and distribution of SALMFamide (S1)-like immunoreactivity (IR), was determined at both the cellular and subcellular level in the central nervous system (CNS) of the nematode roundworm Ascaris suum. The techniques of indirect immunofluorescence in conjunction with confocal scanning laser microscopy and post-embedding, IgG-conjugated colloidal gold immunostaining were used, respectively. Immunostaining was widespread in the CNS of adult A. suum, with immunoreactivity (IR) being localized in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures. At the subcellular level, gold labeling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the neuropile of the anterior nerve ring, main ganglia and nerve cords in the CNS. Double-labeling demonstrated an apparent co-localization of S1- and FMRFamide-IR-together with S1- and pancreatic polypeptide (PP)-IR in the same dense-cored vesicles. Antigen preabsorption experiments indicated little cross-reactivity, if any, between the three antisera; indeed, neither FMRFamide nor PP antigens abolished S1 immunostaining.
Assuntos
Ascaris suum/metabolismo , Sistema Nervoso Central/metabolismo , Neuropeptídeos/metabolismo , Animais , Ascaris suum/citologia , Ascaris suum/imunologia , Ascaris suum/ultraestrutura , Sistema Nervoso Central/ultraestrutura , Feminino , Técnica Indireta de Fluorescência para Anticorpo/métodos , Coloide de Ouro , Masculino , Neuropeptídeos/imunologia , Sensibilidade e Especificidade , Suínos/parasitologiaRESUMO
Historically, peptidergic substances (in the form of neurosecretions) were linked to moulting in nematodes. More recently, there has been a renewal of interest in nematode neurobiology, initially triggered by studies demonstrating the localization of peptide immunoreactivities to the nervous system. Here, David Brownlee, Ian Fairweather, Lindy Holden-Dye and Robert Walker will review progress on the isolation of nematode neuropeptides and efforts to unravel their physiological actions and inactivation mechanisms. Future avenues for research are suggested and the potential exploitation of peptidergic pathways in future therapeutic strategies highlighted.
RESUMO
The localization and distribution of the serotoninergic components of the nervous system in the hydatid organism, Echinococcus granulosus, were determined by immunocytochemical techniques in conjunction with confocal scanning laser microscopy (CSLM). The distribution of serotonin immunoreactivity (IR) paralleled that previously described for cholinesterase activity, although it was more widespread. Nerve cell bodies and nerve fibres immunoreactive for 5-HT were present throughout the central nervous system (CNS), occurring in the paired lateral, posterior lateral and rostellar ganglia, their connecting commissures and nerve rings in the scolex and in the ten longitudinal nerve cords that run posteriorly throughout the body of the worm. A large population of nerve cell bodies was associated with the lateral nerve cords. In the peripheral nervous system (PNS), immunoreactive nerve fibres occurred in well-developed nerve plexuses innervating the somatic musculature and the musculature of the rostellum and suckers. The genital atrium and associated reproductive ducts were richly innervated with serotoninergic nerve cell bodies and nerve fibres.
Assuntos
Echinococcus/química , Serotonina/análise , Animais , Cães , Echinococcus/ultraestrutura , Imuno-Histoquímica , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Sistema Nervoso/química , Sistema Nervoso/ultraestruturaRESUMO
A post-embedding immunogold technique was used to examine the subcellular distribution of immunoreactivities to the invertebrate peptide, FMR-Famide, and to vertebrate pancreatic polypeptide (PP) within the central nervous system of the trematode, Fasciola hepatica. Gold labeling of peptide was localised exclusively over both dense-cored and ellipsoidal electron-dense vesicles (with a homogeneous matrix) present within nerve cell bodies, small and 'giant' nerve processes of the neuropile in the cerebral ganglia and transverse commissure, as well as in the main longitudinal nerve cords. Double labeling demonstrated an apparent co-localisation of FMRFamide and PP immunoreactivities in the same dense-cored vesicles, although populations of ellipsoidal electron-dense vesicles that labeled solely for FMRFamide were also evident. Antigen pre-absorption studies indicated little, if any, cross-reactivity of the two antisera.
Assuntos
Fasciola hepatica/ultraestrutura , Gânglios dos Invertebrados/ultraestrutura , Neuropeptídeos/análise , Polipeptídeo Pancreático/análise , Animais , FMRFamida , Fasciola hepatica/citologia , Gânglios dos Invertebrados/citologia , Imuno-Histoquímica , Hormônios de Inseto/análise , Microscopia Imunoeletrônica , Sistema Nervoso/citologia , Sistema Nervoso/ultraestruturaRESUMO
The pharyngeal component of the enteric nervous system of the parasitic nematode, Ascaris suum exhibits immunoreactivity for serotonin (5-hydroxytryptamine or 5-HT) and for FMRFamide-like peptides. This paper describes the application of an in vitro pharmacological approach to investigate the functional role of 5-HT and FMRFamide-like peptides. The pharyngeal pumping behaviour of Ascaris suum was monitored using a modified pressure transducer system which measures pharyngeal pressure changes and therefore pumping. The pharynx did not contract spontaneously; however, 5-HT (10-1000 microM) stimulated pumping at a frequency of 0.5 Hz. FMRFamide had no apparent effect on pharyngeal pumping. The native nematode FMRFamide-related peptide (FaRP), KSAYMRFamide inhibited the pumping elicited by 5-HT. The duration of inhibition was dose-dependent (0.1-1000 nM) with a threshold of 0.1 nM. In 4 preparations, the inhibition of the pharyngeal muscle was preceded by an initial excitation and increase in the amplitude of pharyngeal pressure changes. The pharynx is involved in various nematode processes, including feeding, regulation of hydrostatic pressure and excretion. The role of 5-HT and KSAYMRFamide in the pharyngeal function of nematodes is discussed.
Assuntos
Ascaris suum/efeitos dos fármacos , FMRFamida , Neuropeptídeos/farmacologia , Músculos Faríngeos/efeitos dos fármacos , Serotonina/farmacologia , Sequência de Aminoácidos , Animais , Ascaris suum/patogenicidade , Ascaris suum/fisiologia , Sistema Nervoso Entérico/efeitos dos fármacos , Sistema Nervoso Entérico/fisiologia , Dados de Sequência Molecular , Contração Muscular/efeitos dos fármacos , Neuropeptídeos/química , Neuropeptídeos/fisiologia , Músculos Faríngeos/fisiologiaRESUMO
The localization and distribution of SALMFamide immunoreactivity (IR), SI(GFNSALMFamide), in the nervous system of both the adult and larval stages of the trematode Schistosoma mansoni has been determined by an indirect immunofluorescent technique in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was widespread in the nervous system of adult male and female S. mansoni. In the central nervous system (CNS), IR was evident in nerve cells and fibres in the anterior ganglia, cerebral commissure and dorsal and ventral nerve cords. In the peripheral nervous system (PNS), IR was apparent in nerve plexuses associated with the subtegmental musculature, oral and ventral suckers, the lining of the gynaecophoric canal, and in fine nerve fibres innervating the dorsal tubercles of the male worm. In the reproductive system of male and female worms, S1-IR was only observed around the ootype/Mehlis' gland complex in the female. Immunostaining was also evident in the nervous system of both miracidium and cercarial larval stages. A post-embedding, IgG-conjugated colloidal gold immunostaining technique was employed to examine the subcellular distribution of SALMFamide-IR in the CNS of S. mansoni. Gold labelling of peptide was localized over dense-cored vesicles within nerve cell bodies and fibres constituting the neuropile of the anterior ganglia, cerebral commissure and nerve cords of the CNS. Antigen pre-absorption studies indicated that the results obtained do suggest S1-like immunostaining and not cross-reactivity with other peptides, in particular FMRFamide.
Assuntos
Sistema Nervoso/química , Neuropeptídeos/análise , Schistosoma mansoni/química , Animais , Especificidade de Anticorpos , Ligação Competitiva , Feminino , Imuno-Histoquímica , Larva/química , Larva/ultraestrutura , Masculino , Microscopia Imunoeletrônica , Neuropeptídeos/imunologia , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/ultraestruturaRESUMO
The localization and distribution of neuropeptides in the central nervous system of the pig roundworm, Ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (IR). Immunostaining was obtained with antisera to pancreatic polypeptide (PP), peptide YY (PYY), neuropeptide Y (NPY), gastrin, cholecystokinin (CCK), substance P (SP), atrial natriuretic peptide (ANP), salmon gonadotropin-releasing hormone (SGnRH), mammalian gonadotropin-releasing hormone (MGnRH), chromogranin A (CGA) and FMRFamide. The most extensive patterns of IR occurred with antisera to PYY, FMRFamide and gastrin. IR was evident in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures; IR to FMRFamide also occurred in the posterior nerve ring. Immunostaining for the other peptides was confined to the nerve cords, with the number of immunoreactive nerve fibres varying from peptide to peptide.