[Standards and criteria in histologic intraoperative diagnosis in the lymph nodes]. / Standardi i kriterijumi intraoperativne histoloske dijagnostike limfnog cvora.

Using frozen sections and classic paraffin sections of the lymph nodes, comparative diagnostic analysis was done in the patients with differently localized malignant tumours. The analysis was based on 86 patients with malignant tumours mostly localized in the maxillofacial region. The frozen sections diagnoses are divided into "metastasing carcinoma in the lymph node", "reactive lymphadenitis", "malignant", "benign", "malignant lymphoma" and "normal lymph node". Diagnostic reproducibility on the level of 90% cases using classic sections was estimated. Negative results in 10% were discussed and some recommendations for improvement of the 9:1 relation were done.

[Stereometric characteristics of inflammatory gingival infiltrates]. / Stereometrijska karakteristika zapaljenjskog infiltrata gingive.

The aim of this paper was to assess stereometric characteristics of gingival cell infiltrate in regard to intensity of clinical inflammation. Materials used for assessment were 70 specimen of human gingiva removed during periodontal surgery therapy. Just before the surgery in the area where specimen was to be taken, intensity of clinical gingival inflammation was determined by gingival index after Loben. Considering stereometric parameters numerical density of lymphocytes and plasma cells were established. Analyzing results it was established that by increase of intensity of clinical gingival inflammation and increase of cell infiltrate occurs, whereas an increase of numerical plasma cells' density is statistically highly important (p < 0.001), while the numerical density of lymphocytes does not changes significantly (p > 0.05).

[Evolution of chronic lymphocytic thyroiditis in primary malignant lymphoma in an extranodal location]. / Evolucija hronicnog limfocitnog tireoiditisa u primarni maligni limfom ekstranodalne lokalizacije.

Primary malignant lymphoma of extranodal localization is a rare disease which mainly occurs in organs with greater or less quantity of lymphocytic tissue or in the organs with the previous history of lymphocytic infiltrate. Extranodal localization of malignant lymphoma in the thyroid gland is rare and numerous authors associate it with the previous existence of chronic lymphocatic thyroiditis (Hashimoto struma). This paper dealt with the histologically analysed material obtained by subtotal or total thyreoidectomy over one-year period. Out of 49 cases, histologic diagnosis of chronic diffuse lymphocytic thyroiditis was established in nine patients (18%) but the frequency of the appearance of this autoimmune disease may well be grater because a relatively small number of patients underwent the operation. Apart from all morphologic characteristics of Hashimoto struma, primary malignant lymphoma of extranodal localization in the thyroid gland was diagnosed in one patient. Simultaneous or previous evolution of chronic lymphocyttic thyroiditis into malignant lymphoma was confirmed in the papers of numerous authors. The authors presented a patient aged 58 in whom malignant lymphoma had evolved from the chronic diffuse lymphocytic thyroiditis. Cellular characteristics pointed to lymphoma of transformed cells being extended extrathyroidically into the surrounding connecting and muscular tissue with the involvement of regional lymph glands. Of particular importance was the area in which the usual architectonics of lymphocytic thyroiditis was discretely ruined and the infiltrate received neoplastic characteristics. On the basis of data from literature, lymphocytic thyroiditis should be regarded as a prelymphomatous state which was attached a great importance from the aspect of histopathologic analysis. Beside the operation, conservative therapy was employed and it showed good results until now, although it was too early to make decisive conclusions on the outcome of disease.

[The present approach to the morphologic analysis of lymph nodes draining a tumor area]. / Savremeni pristup morfoloskoj analizi limfnih cvorova koji dreniraju podrucje tumora.

The paper reviews the results of an extensive morphological analysis of 49 lymph nodes draining the area of histologically evidenced tumor on the basis of immunomorphological changes by the quantitative and semiquantitative methods. Reactive changes in different topographic zones being a morphological manifestation of the immune response to the presence of tumor depend on the type and localization of the tumor. Sinus histiocytosis is the most significant morphological reaction in the analyzed draining nodes. The intensity of sinus histiocytosis could, by itself, represent an important factor in a morphological recognition of the lymph node draining the tumor area.

[The zona fasciculata of the adrenal glands 60 days after termination of exposure to chemical fertilizer]. / Snopovna zona nadbubreezne zlezde 60 dana nakon prestanka delovanja hemijskog gnojiva.

We detected fatty substances as well as enzymic activity in rats which, after a stay in workshop, where NPK fertilizer is produced, for 30 days survived another 60 days in normal conditions. The quantity of neutral fats and tryglycerides was reduced while the content of phospholipids was increased concerning the fasciculate's zone cells. After exposure to NPK fertilizer the cells nuclei got enlarged and dense. The activity of acid phosphatases increased, MAO was reduced, while the activity of succinat-dehydrogenesis was increased. The dermined quantities of fatty substances as well as the enzyme activity point to stimulated activity of fasciculate's zone cells.

Effects of nonsteroidal antiinflammatory drugs on renal plasma flow and glomerular filtration rate in patients with glomerulonephritis.

We investigated the effects of NSAIDs (Ibuprofen) on renal plasma flow (RPF) and glomerular filtration rate (GFR) in patients with glomerulonephritis. The study included 98 patients (53 men and 45 women) with idiopathic glomerulonephritis. All of the patients received Ibuprofen 1200 mg (3x400) during 7 days. Before and after the treatment with Ibuprofen ERPF (clearance of 131I-Hippuran) and GFR (clearance of 99mTc-DTPA) were determined. In our patients administration of Ibuprofen was associated with a significant reduction in renal plasma flow and glomerular filtration rate. In patients with reduced renal function the decrease in RPF and GFR after Ibuprofen was significantly greater than in patients with normal renal function.

[The effect of chronic alcohol administration on specific features of the adenohypophysis in young animals in relation to the adult adenohypophysis]. / Specificnosti adenohipofize mladunaca u odnosu na adenohipofizu odraslih pod hronicnim uticajem alkohola.

We investigated chronic effects of ethyl alcohol on adenohypophysis in adult Wistar rats and adenohypophysis in development including the postnatal period up to 30 days. Morphological findings in gonadotropic, prolactin and follicular cells were compared. After three months of constant alcohol consumption in the form of a 15% liquid solution, vacuolization of gonadotropic cells which acquire the shape of a seal ring, as well as nodular hyperplasia of chromophobe cells combined with sparse somatotropic cells, occur in the adenohypophysis of adult male rats. Electronic microscope revealed a small quantity of prolactin granules in light-microscopic chromophobe cells. In young rats exposed to alcohol gonadotropic and prolactin cells are unchanged but an accumulation of follicular cells within multiple smaller pseudofollicles is present. In the explanation of the differences between the findings obtained in adult and young rats with reference to LH/FSH and PRL cells, a stress is given to the role of the peripheral metabolism of testosterone and of hyperestrogenemia caused by alcohol consumption in adult rats lacking in young ones. The increased number of the follicular cells in adenohypophyses of young rats is likely to reflect the suppressed maturation of hypophyseal secretory cells.