Fluorescent/Colorimetric Dual-Mode Discriminating Gln and Val Enantiomers Based on Carbon Dots.

Discrimination and quantification of amino acid (AA) enantiomers are particularly important for diagnosing and treating diseases. Recently, dual-mode probes have gained a lot of research interest because they can catch more detecting information compared with the single-mode probes. Thus, it is of great significance to develop a dual-mode sensor realizing AA enantiomer discrimination conveniently and efficiently. In this work, carbon dot L-TCDs were prepared by N-methyl-1,2-benzenediamine dihydrochloride (OTD) and l-tryptophan. With the assistance of H2O2, L-TCDs show an excellent discrimination performance for enantiomers of glutamine (Gln) and valine (Val) in both fluorescent and colorimetric modes. The fluorescence enantioselectivity of Gln (FD/FL) and Val (FL/FD) is 5.29 and 4.13, respectively, and the colorimetric enantioselectivity of Gln (ID/IL) and Val (IL/ID) is 13.26 and 3.42, individually. The chiral recognition mechanism of L-TCDs was systematically studied. L-TCDs can be etched by H2O2, and the participation of AA enantiomers results in different amounts of the released OTD, which provides fluorescent and colorimetric signals for identifying and quantifying the enantiomers of Gln and Val. This work provides a more convenient and flexible dual-mode sensing strategy for discriminating AA enantiomers, which is expected to be of great value in facile and high-throughput chiral recognition.

BSA Capped gold Nanoclusters Modulated by Copper ion for Sensitive and Selective Detection of Histidine in Biological Fluid.

This research proposed a sample and environmentally sustainable technique for the synthesis of bovine serum albumin capped gold nanoclusters (BSA-AuNCs) with outstanding fluorescence. The synthesized BSA-AuNCs were investigated using various ways before being combined with Cu2+ to produce a fluorescent switch probe (BSA-AuNCs-Cu2+) for histidine determination. After adding Cu2+, the fluorescence of the BSA-AuNCs was quenched, the fluorescence intensity was enhanced after adding histidine due to good coordination between Cu2+ and histidine. The significant chelation of histidine with Cu2+ demonstrated the viability of developing a selective "switch on" probe for histidine detecting over other amino acids. Unlike existing fluorescent nanomaterial-based approaches for detecting histidine, this study promises good selectivity, high efficiency, and the avoiding of chemical solvents. The designed BSA-AuNCs-Cu2+ fluorescent probe demonstrated an acceptable linear detection range of 0 to 240 µM under optimum circumstances, with a detection limit of 0.9 µM. The BSA-AuNCs-Cu2+ system was investigated in rat serum and human urine, with recoveries ranging from 97.2 to 108.2%, demonstrating its potential applicability for histidine detection with favorable results.