Genetic diversity among coffee tree progenies Big Coffee VL based on growth traits and production.

In a coffee plantation of a coffee 'Acaiá' cultivar (Coffea arabica), on the Midwest of Minas Gerais in Capitólio city, a different kind of coffee tree was found (1989), possibly due to a mutation. It presented larger leaves and grains than those of conventional coffee trees and was named as "Big Coffee VL." The aim of this study was to estimate the genetic diversity of Big Coffee VL progenies cultivated at Universidade Federal de Lavras, by evaluating growth and production traits, based on genetic distances and clusters. The experiment was established in a lattice design with 100 progenies of this coffee tree and 23 repetitions. Traits evaluated were vigor, plant height, stem diameter, node number of plagiotropic branches, pair numbers of plagiotropic branches, and productivity. Genetic divergence was evaluated by multivariate procedures: Mahalanobis generalized distance, clustering methods, and principal component analysis. Genetic distances were estimated using Mahalanobis distance and presented variations from 0.04 to 18.70. The most similar progenies were P23 and P29 and the most dissimilar progenies were G8 and P14. The progenies were divided into three groups, with P14 present as an isolated group. Thus, it was possible to observe the existence of genetic variability among the progenies of Big Coffee VL, which can be used in breeding programs to increase grain size. Progenies G8 and P14 presented the highest genetic distance, and were the most suitable for future integration of crossings in plant breeding programs.

[Distal gastric carcinoma with duodenal invasion. Histopathologic study and review of the literature]. / Invasão do duodeno por carcinoma do terço distal do estômago. Estudo histopatológico e revisão da literatura.

Gastric carcinoma with duodenal invasion is reported in 11% to 33.3% of surgical specimens. In spite of this high frequency, it is not easily recognised during the surgical proceeding or at gross examination. The study of risk factors like histological type, tumor stage and extension of duodenal invasion can be useful in establishing the best surgical approach in order to diminish the risk of local recurrence. We report 50 cases of distal gastric carcinoma in which we analysed the tumor extension in the different layers of the duodenal wall; duodenal invasion was correlated with histological type, level of infiltration in the gastric wall and presence of vascular invasion. Duodenal invasion was observed in 27 cases (54%), 17/32 of intestinal type (53%), 9/10 of diffuse type (90%) e 1/8 of non-classifiable tumours (12.5%). Diffuse type carcinoma was the most important risk factor for invasion (OR = 11; CI 95%: 1.20 to 254.16; P < 0.01). Most of the cases (21/27, 77%) were stage III or IV. The submucosal layer was the most frequent (22/27 cases, 81%) and also most extensively (8.21 +/- 9.75 mm) invaded. We conclude that the risk of duodenal invasion is higher in diffuse type tumours and in stage III or IV. Distal surgical resection should be wider in these cases and determined by frozen section biopsy specimen at the point of transection.

Avaliação dos exames laboratoriais para hepatite B (HBV) em doadores de sangue, HEMEPAR, Secretaria Estadual de Saúde, 1995 / Laboratorial tests evaluation for hepatitis B (HBV) in blood donors

O presente estudo pretende avaliar o perfil soroepidemiológico para HBV em doadores de sangue do Hemepar, no período de 01/01/95 a 30/06/95, através de exames para HBsAg, Anti-HBc Total e TPG/ALT. Foram analisadas 12.964 amostras sorológicas provenientes das seguintes regiões: Curitiba, Paranaguá, Irati, Tibagi, Telêmaco Borba, Ponta Grossa, União da Vitória e Umuarama. O)s exames foram realizados no laboratório do HEMEPARA (Curitiba) utilizando-se método imunoenzimático para HbsAg e anti-HBc e método colorimétrico quantitativo para determinação de TGP/ALT. A soropositividade encontrada foi de 10,03% para anti-HBc, 0,66% para HBsAg e 0,97% de amostras com valor alterado para TGP/ALT. Os doadores com amostras positiva para anti-HBc retornaram posteriormente para nova coleta e exames. Sendo que 85% destas confirmaram-se positivas para anti-HBc e 69% para Anti-HBs. O anti-HBc é o primeiro anticorpo a aparecer no sangue ao final do período de incubação e aí estará presente definitivamente. A sua determinação permitirá identificar se o doador já foi ou está infectado pelo HBV, oferecendo, portanto, proteção ao receptor. O HBsAg está presente por um período curto, podendo negativar até mesmo no período de incubação. A determinação de anti-Hbc total em doadores de sangue é de fundamental importância: 1°) para resguardar a saúde do receptor contra o HBV; 2°) para estudos epidemiológicos, por trabalhar com uma considerável e constante amostragem da população, visando a medidas profiláticas de imunização em pacientes com Anti-HBs negativo.