RESUMO
Osteopontin (OPN) and epidermal growth factor receptor (EGFR) are important factors associated with tumor progression, invasion and metastasis in humans. The aim of this study was to assess the correlation of OPN and EGFR expression with hepatocellular carcinoma (HCC) progression. Expression of OPN and EGFR was assessed by immunohistochemistry in 100 HCC specimens. Immunostaining scores (0 to 400) were calculated from the percentage of cells (0 to 100) at each immunostaining intensity and the immunostaining intensity (0 to 4). The average immunostaining score for OPN was correlated with tumor grade (56.1 for grade I, 104.6 for grade II, and 141.2 for grade III; P = 0.023) and T stage (58.6 for stage T1, 85.9 for stage T2, 126.8 for stage T3, and 189.1 for stage T4; P = 0.029). Similarly, the average immunostaining score for EGFR was correlated with tumor grade (80.5 for grade I, 142.1 for grade II, 230.6 for grade III; P = 0.011) and T stage (96.4 for stage T1, 135.5 for stage T2, 221.3 for stage T3, and 261.4 for stage T4; P = 0.026). In addition, OPN and EGFR immunostaining scores were also correlated with M, N, and AJCC stages. In conclusion, higher expression of OPN and EGFR is significantly associated with advanced histological grades, advanced pathological stages and poorer survival rates in HCC. OPN and EGFR may be used as novel biomarkers for diagnosis or monitoring of progression of hepatocellular carcinoma.
Assuntos
Carcinoma Hepatocelular , Osteopontina , Progressão da Doença , Humanos , Imuno-Histoquímica , Neoplasias HepáticasRESUMO
BACKGROUND: Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. The prognosis of OSCC is usually poor because of extensive local invasion at initial diagnosis. In the literature, Fascin has been reported responsible for cell motility and over-expression of Fascin contributes to an unfavorable clinical course. Nevertheless, the roles of Fascin protein playing in aggressiveness of OSCC and their potential mechanisms need to be elucidated. METHODS: Two cell lines of OSCC (OECM-1 and SCC-25) via the vector-based small interfering RNA (siRNA) to suppress the expression of the Fascin gene were used. Subsequent analyses and observation regarding the expression of Fascin protein and cyto-morphological alterations were detected by Western blot and immunofluorescent microscopy. Boyden chamber invasion assay, cell migration assay and adhesion assay were also applied to investigate the functional changes of OSCC. RESULTS: There were statistically significant differences (P < 0.05) of Fascin expression before and after silencing. Down-regulation of Fascin protein directly led to changes of cell surface protrusions under immunofluorescent microscopy and resulted in suppression of migration, invasion and increase of adhesion in both cell lines (P < 0.05). Furthermore, down-regulation of Fascin expression also resulted in alterations of E-cadherin, beta-catenin and TWIST at certain level, implicative of an association with epithelial-mesenchymal transition (EMT). CONCLUSIONS: Our results suggest that expression of Fascin protein may play an essential role in regulation of progression of OSCC and contributes to the event of EMT in the early aggressiveness of OSCC.
Assuntos
Carcinoma de Células Escamosas/genética , Proteínas de Transporte/genética , Proteínas de Transporte/fisiologia , Proteínas dos Microfilamentos/genética , Proteínas dos Microfilamentos/fisiologia , Neoplasias Bucais/genética , Invasividade Neoplásica/genética , Western Blotting , Caderinas/biossíntese , Carcinoma de Células Escamosas/metabolismo , Proteínas de Transporte/biossíntese , Adesão Celular , Linhagem Celular Tumoral , Ensaios de Migração Celular , Transformação Celular Neoplásica/genética , Regulação para Baixo , Células Epiteliais/patologia , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Mesoderma/patologia , Proteínas dos Microfilamentos/biossíntese , Neoplasias Bucais/metabolismo , RNA Interferente Pequeno/fisiologia , Transfecção , Proteína 1 Relacionada a Twist/biossíntese , beta Catenina/biossínteseRESUMO
AIM: To test the association of LMX1A and osteopontin (OPN) expression with histologic differentiation or pathologic stage in pancreatic ductal adenocarcinoma. METHODS: Immunohistochemical analysis was performed to determine LMX1A and OPN expression in 100 surgical specimens obtained from Chinese patients with well-differentiated (n=15), moderately differentiated (n=65), and poorly differentiated (n=20) pancreatic ductal adenocarcinomas. RESULTS: LMX1A and OPN immunoreactivities were undetectable in normal pancreatic glandular epithelia. Stronger immunostaining for LMX1A and OPN was associated with advanced nuclear grades of pancreatic ductal adenocarcinomas (70.7 and 87.1 for grade I, 109.8 and 118.3 for grade II, and 171.3 and 183.8 for grade III), and advanced TNM and American Joint Committee on Cancer stages of pancreatic ductal adenocarcinomas. CONCLUSIONS: A higher expression of LMX1A and OPN is well correlated with histologic grade and pathologic stage of pancreatic ductal adenocarcinomas.