RESUMO
The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be found along with free MSEL-neurophysin adn copeptin. Guinea pig copeptin has been sequenced. It is a glycopeptide composed of 38 amino acid residues rather than the 39 found in other mammalian copeptins. Compared with other copeptins, that from guinea pig shows a few substitutions and the deletion of one acidic residue, probably in position 32. This deletion might be responsible for incomplete cleavage by the trypsin-like processing enzyme.
Assuntos
Glicopeptídeos/análise , Neuro-Hipófise/análise , Sequência de Aminoácidos , Aminoácidos/análise , Animais , Carboidratos/análise , Quimotripsina , Cobaias , Pepsina A , Fragmentos de Peptídeos/análise , Especificidade da Espécie , TripsinaRESUMO
The guinea pig two-domain precursor of MSEL-neurophysin and copeptin has been passed through a trypsin-Sepharose column in order to mimic the enzyme processing by a membrane-bound endopeptidase. Only two cleavages were observed located in the inter-domain sequence (at Arg-94 and Arg-98), in contrast to several additional cleavages found when free neurophysin or copeptin is subjected to soluble trypsin. Because the physiological maturation involves a single cleavage at Arg-94, both local accessibility in the precursor and narrow specificity of the enzyme are implied in the processing.
Assuntos
Arginina Vasopressina/metabolismo , Glicopeptídeos/metabolismo , Neurofisinas/metabolismo , Ocitocina , Precursores de Proteínas/metabolismo , Tripsina/metabolismo , Sequência de Aminoácidos , Animais , Cromatografia de Afinidade , Cromatografia em Agarose , Cobaias , Hidrólise , Processamento de Proteína Pós-TraducionalRESUMO
The vasopressin precursor is composed of 3 domains, namely vasopressin, MSEL-neurophysin and a glycopeptide. Processing occurs during axonal transport from hypothalamus to neurohypophysis from which the 3 fragments can be isolated. The glycopeptide fragment of the rat vasopressin precursor has been purified and sequenced. Despite the fact that rat MSEL-neurophysin is shortened (93 residues instead of 95 for other mammals), rat glycopeptide has 39 residues, as do the other mammalian glycopeptides, suggesting a similar processing. Fifteen substitutions are however observed when compared to ox glycopeptide. The C-terminal part of MSEL-neurophysin (residues 77-93) and the glycopeptide are encoded by the same exon and the homologies when compared with their bovine counterparts are 58% and 62% respectively. In contrast, the central part of rat MSEL-neurophysin (residues 10-76), which is encoded by a separate exon, displays 96% of homology; vasopressin and the N-terminal part of MSEL-neurophysin (residues 1-9), encoded by a third exon, are nearly invariant.
Assuntos
Glicopeptídeos/isolamento & purificação , Precursores de Proteínas/análise , Vasopressinas/análise , Sequência de Aminoácidos , Animais , Bovinos , Humanos , Ratos , Especificidade da EspécieRESUMO
Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the reptile. This result confirms the evolutionary specificity of hydrin 2 that has been identified only in frogs and toads but not in birds and reptiles. Occurrence of hydrin 2 is explained by its regulatory function on the water permeability of the skin of anurans.
Assuntos
Evolução Biológica , Neuropeptídeos/análise , Hipófise/análise , Vasotocina/análogos & derivados , Animais , Bufo marinus , Cromatografia Líquida de Alta Pressão , Ocitocina/análogos & derivados , Ocitocina/isolamento & purificação , Serpentes , Especificidade da Espécie , Vasotocina/análise , Vasotocina/isolamento & purificaçãoRESUMO
Guinea pig neurohypophysial hormones have been purified by two procedures, one involving molecular sieving and paper chromatoelectrophoresis, the other high-pressure reverse-phase liquid chromatography. Arginine vasopressin and oxytocin have been identified by their amino acid compositions and their retention times in HPLC determined through their biological properties. No partially processed precursor, including a neurohormone and a neurophysin, has been detected. Because the cleavage of the three-domain vasopressin-neurophysin-copeptin precursor is apparently complete between the first two domains, whereas it is not between the second and the third, it is supposed that two distinct enzymic systems are involved in the processing.
Assuntos
Hormônios Neuro-Hipofisários/isolamento & purificação , Vasopressinas/genética , Sequência de Aminoácidos , Animais , Cromatografia em Gel/métodos , Cromatografia Líquida de Alta Pressão/métodos , Eletroforese em Papel/métodos , Cobaias , Neuro-Hipófise/análise , Hormônios Neuro-Hipofisários/genéticaRESUMO
A 'big' frog (Rana esculenta) neurophysin, encompassing sequences homologous to mammalian MSEL-neurophysin and copeptin, has been passed through a trypsin-Sepharose column in order to compare its conformation with that of the two-domain intermediate precursor isolated from guinea pig. Whereas the polypeptide possesses 8 arginine residues, only two cleavages were observed located in a putative inter-domain sequence (at Arg-94 and Arg-114). Because free vasotocin has been isolated from the frog, it is assumed that pro-vasotocin has a three-domain conformation similar to that of pro-vasopressin but processing in amphibians involves only one step rather than two steps as in mammals.
Assuntos
Arginina Vasopressina , Glicopeptídeos , Neurofisinas , Ocitocina , Precursores de Proteínas , Sequência de Aminoácidos , Animais , Evolução Biológica , Bovinos , Dados de Sequência Molecular , Conformação Proteica , Rana esculenta , Homologia de Sequência do Ácido Nucleico , VasopressinasRESUMO
The neurohypophysial hormones of the 1-month-old bovine fetus have been identified by their positions in ion-exchange chromatography and their retention times in high-pressure reverse-phase partition chromatography. Arginine vasopressin and oxytocin have been recognized. The molar ratio vasopressin/oxytocin in neurohypophysis is about 6 in the 1-month-old fetus compared with 4 in the 3-month-old fetus, 2.7 in the 7-month-old fetus and 1 in the adult. Vasotocin is virtually absent even in the early fetus (less than 0.1% of arginine vasopressin). The occurrence of a vasotocin gene expressed in the fetus but silent in the adult appears unlikely.
Assuntos
Neuro-Hipófise/embriologia , Hormônios Neuro-Hipofisários/análise , Vasotocina/análise , Animais , Bovinos , Cromatografia Líquida de Alta Pressão , Desenvolvimento Embrionário e FetalRESUMO
alpha- and beta-melanocyte-stimulating hormones (alpha-MSH and beta-MSH) have been isolated from Xenopus laevis neurointermediate pituitary and microsequenced. Intracellular alpha-MSH is not N-acetylated after proteolytic processing of pro-opiomelanocortin in contrast to mammalian alpha-MSHs. There is a high preservation of the melanotropic amino acid sequence common to all MSHs although in Xenopus beta-MSH a histidine residue replaces the glutamic acid residue found in position 8 of mammalian beta-MSHs.
Assuntos
Hormônios Estimuladores de Melanócitos/análise , Hipófise/análise , Pró-Opiomelanocortina/metabolismo , Xenopus laevis/metabolismo , alfa-MSH/análise , Acetilação , Sequência de Aminoácidos , Animais , Cromatografia Líquida de Alta Pressão , Glutamatos , Ácido Glutâmico , Histidina , Hormônios Estimuladores de Melanócitos/metabolismo , Dados de Sequência Molecular , Peptídeo Hidrolases/metabolismo , Hipófise/metabolismo , Homologia de Sequência do Ácido Nucleico , alfa-MSH/metabolismoRESUMO
The physiological functions of angiotensin I-converting enzyme (ACE) are not limited to its cardiovascular role. ACE constantly degrades N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP), a natural circulating regulator of the hematopoietic stem cell proliferation, and thereby may be involved in hematopoietic stem cell regulation. AcSDKP is hydrolyzed 50-fold faster by the N-domain active site compared to the C-domain active site. The aim of the present study was to investigate which aminoacid residues from AcSDKP are required to ensure N-domain specificity. Several peptides were designed by progressively increasing the length of the peptidic chain from a tripeptide to a pentapeptide. Kinetic studies of the wild-type ACE and of the two ACE mutants containing a single active domain (N- or C-domain) were performed using Bz (benzoyl) Asp-Lys-Pro, benzoyl-glycyl (Bz-Gly)-Asp-Lys-Pro, and Bz-Gly-Ser-Asp-Lys-Pro (with its intermediate product Bz-Gly-Ser-Asp) as substrates. The unexpected importance of an aspartic acid in the P1 position was discovered, as well as the interaction of the P2 and P3 positions in the substrate to increase or decrease N-domain specificity. Substrates longer than five residues may involve interdependence between subsites. Finally, the discovery of highly specific and novel N-domain substrates cannot be predicted from single subsite mapping, but may require other approaches such as combinatorial peptide libraries.
Assuntos
Oligopeptídeos/metabolismo , Peptidil Dipeptidase A/metabolismo , Sítios de Ligação , Cinética , Oligopeptídeos/química , Peptidil Dipeptidase A/química , Peptidil Dipeptidase A/genética , Relação Estrutura-Atividade , Especificidade por SubstratoRESUMO
From guinea pig posterior pituitaries, a MSEL-type neurophysin (neurophysin containing methionine-2, serine-3, glutamic acid-6 and leucine-7), a glycopeptide referred to as copeptin and their common precursor have been purified to homogeneity and sequenced. The performed acid-oxidized precursor, subjected to trypsin hydrolysis, has given 9 peptides, 6 of which (T1-T6) identical to those given by oxidized MSEL-neurophysin except that T6 has an additional C-terminal arginine residue when compared to its homologue. The other 3 tryptic peptides (T7-T9) are identical to those given by copeptin. The 132-residue precursor therefore comprises a MSEL-type neurophysin (93 residues) and copeptin (38 residues) linked by an arginine residue. The molar proportion of this bound form compared with the free polypeptides is approximately 20%. It is believed that this precursor is a part of the vasopressin-MSEL-neurophysin-copeptin precursor incompletely processed during the transport from hypothalamus to neurohypophysis.
Assuntos
Arginina Vasopressina/isolamento & purificação , Glicopeptídeos/isolamento & purificação , Neurofisinas/isolamento & purificação , Ocitocina , Precursores de Proteínas/isolamento & purificação , Sequência de Aminoácidos , Animais , Cobaias , Peso Molecular , Fragmentos de Peptídeos/isolamento & purificação , Neuro-Hipófise/análiseRESUMO
Angiotensin I-converting enzyme (ACE) is a type I transmembrane protein composed of two domains (N and C domains) which undergoes a post-translational proteolytic cleavage in mammalian cells to release the soluble ectodomain. The protease involved in ACE cleavage-secretion (ACE-secretase) is not well characterised and eludes isolation: the presence of a yeast homologue, thus more amenable to genetic manipulation, would facilitate its identification. We have expressed a secreted form of the ACE C domain, lacking the C-terminal membrane anchor (C domain(deltaCOOH)), and the membrane-anchored C domain (C domain) in the yeast Pichia pastoris by fusion to prepro-alpha-factor. Immunofluorescent labelling localises the ACE C domain to the periphery of yeast cells but not C domain(deltaCOOH), however, expression of both C domain and C domain(deltaCOOH) produced soluble enzymes in the culture medium. Immunocharacterisation of the two soluble forms of the C domain indicates a proteolytic cleavage of the membrane-bound C domain to produce the soluble counterpart. Thus ACE undergoes a proteolytic cleavage in yeast.
Assuntos
Endopeptidases/metabolismo , Peptídeos , Peptidil Dipeptidase A/metabolismo , Processamento de Proteína Pós-Traducional , Sequência de Aminoácidos , Clonagem Molecular , Humanos , Cinética , Fator de Acasalamento , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Biossíntese Peptídica , Peptidil Dipeptidase A/biossíntese , Peptidil Dipeptidase A/química , Pichia , Inibidores de Proteases/farmacologia , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Deleção de Sequência , Especificidade por SubstratoRESUMO
Neurosecretory granules have been isolated from goose posterior pituitaries and their contents have been analyzed by reverse-phase high-pressure liquid chromatography and polyacrylamide gel electrophoresis. Vasotocin and mesotocin have been identified by their biological activities and their retention times compared with those of synthetic peptides. MSEL- and VLDV-neurophysins have been characterized by their N-terminal sequences, their electrophoretic migrations and their retention times, compared with those of purified goose neurophysins. In contrast to the two-step processing of mammalian provasopressin, processing of the vasotocin - MSEL-neurophysin precursor appears to involve only one cleavage giving the hormone and a "big" MSEL-neurophysin homologous to mammalian MSEL-neurophysin extended by copeptin.
Assuntos
Grânulos Citoplasmáticos/análise , Gansos/metabolismo , Neuropeptídeos/análise , Neuro-Hipófise/análise , Animais , Arginina Vasopressina/análise , Fracionamento Celular , Cromatografia Líquida de Alta Pressão , Eletroforese em Gel de Poliacrilamida , Neurofisinas/análise , Ocitocina/análogos & derivados , Ocitocina/análise , Precursores de Proteínas/análise , Vasotocina/análiseRESUMO
UNLABELLED: Xenopus laevis neurophysins have been purified from neurointermediate pituitaries through high-pressure reverse-phase liquid chromatography and their N-terminal amino acid sequences have been determined by microsequencing. Two types of neurophysins, corresponding to mammalian MSEL- and VLDV-neurophysins, have been distinguished. A strong homology exists between neurophysins of Xenopus (Pipidae), frog (Ranidae) and toad (Bufonidae). Xenopus MSEL-neurophysin, as frog MSEL-neurophysin, has a high molecular mass suggesting that the C-terminal domain of the vasotocin precursor is not processed in contrast to the two-step processing observed for mammalian vasopressin precursor. ABBREVIATIONS: Mammalian neurophysins are termed MSEL- and VLDV-neurophysins according to the nature of residues in positions 2, 3, 6 and 7 (one-letter symbols for amino acids).
Assuntos
Neurofisinas/isolamento & purificação , Hipófise/química , Sequência de Aminoácidos , Animais , Bufonidae , Bovinos , Cromatografia Líquida de Alta Pressão , Dados de Sequência Molecular , Neurofisinas/química , Rana esculenta , Homologia de Sequência do Ácido Nucleico , Xenopus laevisRESUMO
Neurosecretory granules have been isolated from rat and guinea pig neurointermediate pituitaries and their contents have been analyzed by reverse-phase high-pressure liquid chromatography and polyacrylamide gel electrophoresis. Granule components have been compared with synthetic neurohypophysial hormones and chemically characterized neurophysins. In rat granules, oxytocin, arginine vasopressin and MSEL- and VLDV-neurophysins have been identified. In isolated guinea pig granules, only arginine vasopressin and mature MSEL-neurophysin have been found. From these results it can be concluded that both the "dibasic" cleavage between vasopressin and MSEL-neurophysin and the "monobasic" cleavage between MSEL-neurophysin and copeptin occur within the granule compartment. Previous isolation from frozen guinea pig glands of a partially processed precursor encompassing MSEL-neurophysin and copeptin suggests a two-step processing of the three-domain vasopressin precursor, each involving a distinct enzymic system.
Assuntos
Arginina Vasopressina/isolamento & purificação , Neurofisinas/isolamento & purificação , Neuro-Hipófise/análise , Precursores de Proteínas/isolamento & purificação , Animais , Cromatografia Líquida de Alta Pressão , Grânulos Citoplasmáticos/análise , Cobaias , Ocitocina/isolamento & purificação , RatosRESUMO
Precursors of neurohypophysial hormones are small proteins processed into nonapeptide hormones and neurophysins during axonal transport to the neurohypophysis. In mammals, oxytocin is associated with VLDV-neurophysin and vasopressin with MSEL-neurophysin. In birds, mesotocin and vasotocin are found instead of mammalian oxytocin and vasopressin. From goose, chicken and ostrich posterior pituitary glands, two types of neurophysins related to mammalian VLDV- and MSEL-neurophysins, respectively, have been identified by their N-terminal sequences. It is assumed that, as in mammals, hormonal peptide and the first 9 residues of the corresponding neurophysin are encoded by a common exon and that mesotocin and vasotocin, evolutionary predecessors of oxytocin and vasopressin, are associated in the precursors with VLDV-neurophysin and MSEL-neurophysin, respectively.
Assuntos
Neurofisinas/isolamento & purificação , Ocitocina/análogos & derivados , Precursores de Proteínas/isolamento & purificação , Vasotocina/isolamento & purificação , Sequência de Aminoácidos , Animais , Bovinos , Galinhas , Gansos , Cavalos , Humanos , Ocitocina/isolamento & purificação , Hipófise/análise , Ratos , Ovinos , Suínos , BaleiasRESUMO
Amino acid sequences of the first half of MSEL- and VLDV-neurophysins from goose and chicken have been determined. Identical substitutions in positions 17, 18, 35, 36 and 41 of both neurophysins of a given species when compared with their mammalian counterparts suggest a gene conversion between vasotocin--MSEL-neurophysin and mesotocin--VLDV-neurophysin genes. This event, which has already been observed for three mammalian species, seems recurrent and would link the evolution of the two neurohypophysial hormone precursors.
Assuntos
Conversão Gênica , Ocitocina/análogos & derivados , Vasotocina/genética , Sequência de Aminoácidos , Animais , Evolução Biológica , Galinhas , Gansos , Ligação Genética , Dados de Sequência Molecular , Neurofisinas/genética , Ocitocina/genéticaRESUMO
Present-day marsupials, which are supposed to have arisen from a single stem diverging from the placental stem some 130 million years ago, exist only in the American and Australian continents. Comparison of the homologous genes and their protein products, which evolved under different environmental conditions, may provide arguments for either selective or neutral evolution. In contrast to Australian Macropodidae, which have peculiar neurohypophysial peptides, namely mesotocin and two pressor peptides, lysine vasopressin and phenypressin, the South American oppossum, Didelphis marsupialis, has oxytocin, lysine vasopressin, and arginine vasopressin. Because placental mammals have oxytocin and usually arginine vasopressin, and nonmammalian tetrapods have mesotocin and arginine vasotocin, it is assumed that (1) selective change of arginine vasotocin into arginine vasopressin occurred in mammalian ancestors and a subsequent gene duplication in the marsupial line gave rise to two pressor peptides with divergent neutral drifts in American and Australian groups, and (2) mesotocin of nonmammalian tetrapods has been preserved in Australian marsupials and reclaimed for milk-ejecting function whereas it has been converted into oxytocin in South American oppossums. The change of mesotocin into oxytocin seems neutral rather than selective.
Assuntos
Evolução Biológica , Frequência do Gene , Marsupiais/genética , Proteínas do Tecido Nervoso/genética , Ocitocina/genética , Vasopressinas/genética , Sequência de Aminoácidos , Animais , Austrália , Adeno-Hipófise/fisiologia , América do Sul , Especificidade da EspécieRESUMO
The oxytocin/vasopressin superfamily encompasses vertebrate and invertebrate peptides and therefore the ancestral gene encoding the precursor protein antedates the divergence between the two groups, about 700 million years ago. The preserved nonapeptide pattern indicates that both the precursor structures and the processing enzymatic machinery were greatly conserved to ensure the building of a specific conformation. Substitutions, which may be neutral or selective, occurred in precise positions. Virtually all vertebrate species possess an oxytocin-like and a vasopressin-like peptide so that two evolutionary lineages can be traced. Because a single peptide, vasotocin ([Ile3]-vasopressin or [Arg8]-oxytocin) has been found in the most primitive Cyclostomata, a primordial gene duplication and subsequent mutations are assumed to have given rise to the two lineages. They started with vasotocin and isotocin ([Ser4,Ile8]-oxytocin) in bony fishes and culminated with vasopressin and oxytocin in placental mammals. Mesotocin ([Ile3]-oxytocin), found in lungfishes, amphibians, reptiles, birds and marsupials, appears as an evolutionary intermediate. The change from isotocin ([Ser4,Ile8]-oxytocin) into mesotocin ([Ile8]-oxytocin), can be observed in African and Australian lungfishes, species making the transition from bony fishes to land vertebrates. On the other hand the replacement of mesotocin by oxytocin can be detected in marsupials, particularly in the North-American opossum and the Australian Northern bandicoot that have both mesotocin and oxytocin whereas placental mammals possess only oxytocin. The invariability of this peptide in placentals can be explained by receptor-fitting selective pressure. In contrast to bony vertebrates in which neurohypophysial hormones revealed a remarkable structural stability, cartilaginous fishes displayed an unique oxytocin-like hormone evolution with variability and duality. Aside from vasotocin, in the subclass Selachii, rays have glumitocin ([Gln8-oxytocin]) and sharks possess two peptides: aspargtocin ([Asn4-oxytocin]) and valitocin ([Val8-oxytocin]) for the spiny dogfish, asvatocin ([Asn4,Val8]-oxytocin) and phasvatocin ([Phe3,Asn4,Val8]-oxytocin) for the spotted dogfish. In the other subclass Holocephali, the chimaera (ratfish) has oxytocin, the typical hormone of placental mammals. Cartilaginous fishes used urea rather than salts for their osmoregulation and oxytocin-like hormones could have been relieved from osmoregulatory functions and able to accept many neutral variations.