Whole genome sequencing and the application of a SNP panel reveal primary evolutionary lineages and genomic variation in the lion (Panthera leo).

BACKGROUND: Previous phylogeographic studies of the lion (Panthera leo) have improved our insight into the distribution of genetic variation, as well as a revised taxonomy which now recognizes a northern (Panthera leo leo) and a southern (Panthera leo melanochaita) subspecies. However, existing whole range phylogeographic studies on lions either consist of very limited numbers of samples, or are focused on mitochondrial DNA and/or a limited set of microsatellites. The geographic extent of genetic lineages and their phylogenetic relationships remain uncertain, clouded by massive sampling gaps, sex-biased dispersal and incomplete lineage sorting. RESULTS: In this study we present results of low depth whole genome sequencing and subsequent variant calling in ten lions sampled throughout the geographic range, resulting in the discovery of >150,000 Single Nucleotide Polymorphisms (SNPs). Phylogenetic analyses revealed the same basal split between northern and southern populations, as well as four population clusters on a more local scale. Further, we designed a SNP panel, including 125 autosomal and 14 mitochondrial SNPs, which was tested on >200 lions from across their range. Results allow us to assign individuals to one of these four major clades (West & Central Africa, India, East Africa, or Southern Africa) and delineate these clades in more detail. CONCLUSIONS: The results presented here, particularly the validated SNP panel, have important applications, not only for studying populations on a local geographic scale, but also for tracing samples of unknown origin for forensic purposes, and for guiding conservation management of ex situ populations. Thus, these genomic resources not only contribute to our understanding of the evolutionary history of the lion, but may also play a crucial role in conservation efforts aimed at protecting the species in its full diversity.

NATURALLY OCCURRING RADIONUCLIDES IN SOIL SAMPLES OF BURETI SUB-COUNTY OF KERICHO COUNTY KENYA.

The activity concentration of soil samples of Bureti sub-county was measured using thallium-activated sodium iodide detector. To ascertain the level of radiation hazard to the public, gamma radiation dose rates were also estimated. The average activity concentration due to 40K, 226Ra and 232Th for soil samples are 1164 ± 70, 106 ± 8 and 79 ± 5 Bqkg-1, respectively. An average dose rate of 145 ± 10 nGyh-1 was recorded, which is about 2.5 times higher than the world average value of 60 nGyh-1(UNSCEAR). On the other hand, an average outdoor effective dose of 0.35 ± 0.02 mSvy-1 was measured, which is lower than the ICRP safety limit of 1 mSvy-1. This shows that the radiation hazards from naturally occurring terrestrial radionuclides in Bureti is low and therefore human radiation exposure is within the accepted limits.

HUMAN EXPOSURE TO BACKGROUND RADIATION IN ORTUM, KENYA.

The activity concentration of radionuclides 238U, 232Th and 40K in soil and the absorbed dose rate (ADRA) at 1 m above the ground in Ortum was determined. The activity concentration in soils ranged from 33 to 85, 20 to 67 and 148-1019 Bq kg-1, respectively with an average of 40 ± 1.43, 56 ± 1.46 and 425 ± 19.24 Bq kg-1, respectively. The activity concentration of 232Th and 238U was found to reduce with increasing depth while that of 40K increased with increasing depth. The average activity concentration in soil was higher than the world average values. The average ADRA in air at 1 m above the ground was found to be 112 ± 29.6 nGy h-1. The soil and rocks in Ortum are recommended for use because the activity concentration of the terrestrial radionuclides is lower than the recommended threshold values.

REVIEW OF RADON AND THORON RESEARCH IN KENYA: 1997-2017.

Six research studies involving radon and thoron in Kenya were carried out between 1997 and 2007. The studies were mainly small scale and involved a few areas scattered across Nairobi, Rift Valley, Coast and Western regions. The results were captured in seven online journal articles. This paper relooks at the journal articles with the view of underscoring the high radon and thoron areas in Kenya, raising awareness on the sources and risks of radon isotopes in indoor environments and highlighting the need for more research on the isotopes in the country.

ANNUAL EFFECTIVE DOSE ASSESSMENT DUE TO RADON AND THORON PROGENIES IN DWELLINGS OF KILIMAMBOGO, KENYA.

Human beings are continuously exposed to ionising radiation originating from natural or artificial sources. Uranium-238 and Thorium-232 found in building materials are important sources of radon and thoron in the indoor environment. The concentration levels of radon, thoron and thoron progeny were measured in mud-walled, metallic or iron sheet-walled and stone-walled modern houses in Kilimambogo region, Kenya for 3 months. Radon and thoron concentration levels were measured using passive radon-thoron discriminative monitors (RADUET), while thoron progeny concentrations as the equilibrium equivalent thoron concentration (EETC) were measured using thoron progeny monitors. The mean radon concentration levels in mud, metallic and stone-walled dwellings were 67 ± 11, 60 ± 10 and 75 ± 10 Bq m-3, respectively. The mean thoron concentration levels in the corresponding dwellings were 195 ± 36, 71 ± 24 and 161 ± 31 Bq m-3, respectively, while EETCs were 12 ± 2, 3 ± 1 and 7 ± 1 Bq m-3, respectively. The annual effective doses for radon were 1.3 ± 0.2, 1.1 ± 0.1 and 1.4 ± 0.2 mSv y-1 in mud, metallic and stone-walled houses while those from thoron estimated from EETC were 2.4 ± 0.4, 0.5 ± 0.1 and 1.5 ± 0.2 mSv y-1 in the corresponding houses, respectively.

Household air pollution in Nairobi's slums: A long-term policy evaluation using participatory system dynamics.

58% of Nairobi's population live in informal settlements in extremely poor conditions. Household air pollution is one of the leading causes of premature death and disease in these settlements. Regulatory frameworks and government budgets for household air pollution do not exist and humanitarian organisations remain largely inattentive and inactive on this issue. The purpose of this paper is to evaluate the effectiveness of potential indoor-air related policies, as identified together with various stakeholders, in lowering household air pollution in Nairobi's slums. Applying a novel approach in this context, we used participatory system dynamics within a series of stakeholder workshops in Nairobi, to map and model the complex dynamics surrounding household air pollution and draw up possible policy options. Workshop participants included community members, local and national policy-makers, representatives from parastatals, NGOs and academics. Simulation modelling demonstrates that under business-as-usual, the current trend of slowly improving indoor air quality will soon come to a halt. If we aim to continue to substantially reduce household PM2.5 levels, a drastic acceleration in the uptake of clean stoves is needed. We identified the potentially high impact of redirecting investment towards household air quality monitoring and health impact assessment studies, therefore raising the public's and the government's awareness and concern about this issue and its health consequences. Such investments, due to their self-reinforcing nature, can entail high returns on investment, but are likely to give 'worse-before-better' results due to the time lags involved. We also discuss the usefulness of the participatory process within similar multi-stakeholder contexts. With important implications for such settings this work advances our understanding of the efficacy of high-level policy options for reducing household air pollution. It makes a case for the usefulness of participatory system dynamics for such complex, multi-stakeholder, environmental issues.

Analysis of internal exposure associated with consumption of crops and groundwater from the high background radiation area of Mrima Hill, Kenya.

Specific concentrations of (226)Ra, (232)Th and (40)K were measured in cassava tubers, cassava leaves and groundwater obtained from the high background radiation area of Mrima hill and used in the evaluation of the ingested dose. Cassava tubers, cassava leaves and groundwater registered average (226)Ra concentrations of 60 ± 5, 141 ± 11 and 4.3 ± 0.3 Bq kg(-1), respectively. (232)Th was not detected in cassava leaves although it was present in cassava tubers as well as in groundwater in average concentrations of 35.3±61.5 and 2.0±0.1 Bq kg(-1), respectively. (40)K was present in all samples in average concentrations of 842±539 Bq kg(-1) in cassava tubers, 1708 ± 552 Bq kg(-1) in cassava leaves and 91.4 Bq kg(-1) in groundwater. The total annual effective dose due to ingestion was found to be 7.9 mSv y(-1) of which 2.4 mSv y(-1) was due to cassava tubers, 3.8 mSv y(-1) due to cassava leaves and 1.7 mSv y(-1) due to water.

Estimation of annual effective dose due to radon and thoron concentrations in mud dwellings of Mrima Hill, Kenya.

This study presents radon and thoron concentration measurements and the corresponding effective dose rates in mud dwellings located in the high background radiation area of Mrima Hill, Kenya. Discriminative technique was used for simultaneous measurement of radon and thoron. The effective dose was evaluated based on the concentration of the isotopes and the time spent indoors. Radon concentration ranged from 16 to 56 Bq m(-3) with an average of 35±14 Bq m(-3) and a corresponding annual effective dose of 0.67 mSv y(-1), while that of thoron ranged from 132 to 1295 Bq m(-3) with an average of 652±397 Bq m(-3) and an effective dose of 13.7 mSv y(-1).

Childcare practices of commercial sex workers.

OBJECTIVE: To determine the childcare practices of commercial sex workers (CSWs). DESIGN: A descriptive cross-sectional survey was conducted between July and December 2000 during which a structured questionnaire was administered. SETTING: Kibera slum, Nairobi, Kenya. SUBJECTS: Three hundred eighty five CSWs and four focus group discussions (FGDs) held. Health cards from 126 under five years old children belonging to the respondents were reviewed for immunization status and regularity of growth monitoring. RESULTS: The mean age of the 385 CSWs surveyed was 32 +/- 7 years and mean duration of sex work was 6 +/- 4 years. The mean number of living children was 3.4 +/- 2 and 81.2% of the mothers lived with their children. Three quarters of the CSWs practised prostitution at home. The most common daily childcare activities by the mothers were food preparation (96.2%) and washing children's clothes (91.3%). Overall 96.8% of their under-five years old children were fully immunized and 80% of their under one year old children had their growth monitored monthly. About three quarters of the mothers with adolescent children educated them on HIV/STDs. Health seeking behaviour for the children was hampered by health care cost (71.4%) and consumption of alcohol by the mothers. Like other mothers, the CSWs encouraged their adolescent children to take up some adult roles such as maintaining a clean house (93.3%). However only 2.0% took time to converse or counsel the children. Focus group discussions (FGDs) with the CSWs showed that children were left unattended at night while the mothers went out in search of clients. Efforts to provide better education for the children were undermined by lack of funds (52.2%) and truancy (46.6%). One third of the study population had invested for the future maintenance of their children. CONCLUSION: There was more emphasis on physical, rather than psychological aspect of childcare. The practice of living with the children ensured that earnings from the sex trade were used for the immediate needs of the children such as food. However this practice had a negative influence on the children as the majority of the respondents conducted their sexual business at home with little or no privacy. Health seeking behaviour for the children was hampered by lack of funds and to some extent alcohol consumption by the mothers. Efforts to invest in the education of their children were undermined by lack of funds and truancy.

The influence of meteorological parameters on indoor radon in selected traditional Kenyan dwellings.

The effects of outdoor temperature, rainfall and wind speed on the indoor radon concentration in four types of traditional hut in Kenya were investigated by performing short-term (about 48 h) measurements with activated charcoal canisters. The concentrations vary widely (30.2-315.4 Bq m(-3)) during the study period, and the overall mean is 170.3 +/- 39.6 Bq m(-3). The indoor radon concentration correlates negatively with both the outdoor temperature (R(2) = 0.06) and the wind speed (R(2) = 0.11) but positively with rainfall (R(2) = 0.03). The results showed that changes in meteorological parameters cause more variations in indoor radon concentrations than the differences in the buildings' characteristics (i.e., designs, materials, etc). However, these variations are not significant (at 1% significance level).