RESUMO
Viral infections, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are some of the most dangerous threats to humans. SARS-CoV-2 has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. To meet these requirements, we designed a label-free colorimetric platform that combines the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas) 12a system for naked-eye detection (named LFP). This method utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the trans-cleavage activity of the CRISPR/Cas12a system to increase the sensitivity and specificity of the reaction. This platform can detect as few as 4 copies/µL of RNA and produces no false positive results when tested against the influenza virus. To better meet the requirements of point-of-care (POC) detection, we developed a portable device that can be applied in resource-poor and densely populated regions. The LFP assay holds great potential for application in resource-limited settings, and the label-free gold nanoparticle (AuNPs) probe can reduce costs, making it suitable for large-scale screening. We expect that the LFP assay will be promising for the POC screening of COVID-19.
Assuntos
Colorimetria , Ouro , Nanopartículas Metálicas , Técnicas de Amplificação de Ácido Nucleico , RNA Viral , SARS-CoV-2 , Ouro/química , Colorimetria/métodos , Colorimetria/instrumentação , Nanopartículas Metálicas/química , SARS-CoV-2/genética , SARS-CoV-2/isolamento & purificação , RNA Viral/análise , RNA Viral/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Técnicas de Amplificação de Ácido Nucleico/instrumentação , Humanos , COVID-19/diagnóstico , COVID-19/virologia , Sistemas CRISPR-Cas , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Técnicas de Diagnóstico MolecularRESUMO
A green photo-oxidation reaction was discovered in which the imidazole ring is oxidized and rearranged to generate imidazolinone derivatives with thermal activation delayed fluorescence properties. The excellent photo-oxidation properties of p-PTZ-PIM can be utilized for detecting trace oxygen in the water phase and the sealing of food packaging bags.
RESUMO
We developed an asymmetric decarboxylative allylic alkylation of vinylethylene carbonates with α-fluoro pyridinyl acetates through a synergistic palladium/copper catalysis. This protocol provides chiral allylic alcohol with carbon-fluorine quaternary stereogenic centers in good yield with good enantioselectivities and excellent regioselectivities. The utility of this approach was further demonstrated via a gram-scale experiment and derivatizations of the product.