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1.
Pak J Pharm Sci ; 37(1(Special)): 205-213, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38747271

RESUMO

In this study, a sensitive high-performance liquid chromatography detector was established and validated for the simultaneous determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. The analysis was achieved on CHANIN 100-5-C18-H column (5µm, 250 mm×4.6 mm) with the temperature of 30oC. Gradient elution was applied using 0.1% phosphoric acid solution-methanol-acetonitrile (50:50) as mobile phase at the flow rate of 1.0 mL/min. The determination was performed at the wavelength of 225 nm (detecting geniposide), 254 nm (detecting ellagic acid), 343 nm (detecting piperine) and 225 nm (detecting costunolide and dehydrocostuslactone) along with the sample volume of 10µL. The linear ranges of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone demonstrated good linear relationships within their respective determination ranges. The average recoveries were 100.04%, 99.86%, 99.79%, 100.17% and 100.41%, respectively. RSD% was 1.3%, 1.2%, 1.2%, 1.2%, 1.5%, respectively. The developed method was proved to be simple, accurate and sensitive, which can provide a quantitative analysis method for the content determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang capsules.


Assuntos
Alcaloides , Benzodioxóis , Cápsulas , Medicamentos de Ervas Chinesas , Ácido Elágico , Iridoides , Lactonas , Piperidinas , Alcamidas Poli-Insaturadas , Cromatografia Líquida de Alta Pressão/métodos , Benzodioxóis/análise , Alcamidas Poli-Insaturadas/análise , Piperidinas/análise , Piperidinas/química , Alcaloides/análise , Lactonas/análise , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/análise , Iridoides/análise , Ácido Elágico/análise , Reprodutibilidade dos Testes , Sesquiterpenos/análise
2.
Pak J Pharm Sci ; 36(5(Special)): 1597-1607, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38008958

RESUMO

A rapid, highly specific and sensitive UPLC-MS/MS method was developed for the determination of Quetiapine Fumarate, a therapeutic drug for various psychiatric disorders, in human plasma. The samples were pretreated using a protein precipitation method, followed by chromatographic separation using a column (Kinetex C18, 2.6µm 50*2.1mm) equipped with an ESI source and MRM mode mass spectrometer. In the validation results of the method, the analyte quetiapine showed a peak at approximately 1.0 minute and exhibited good linearity within the concentration from 2.5 to 2000ng/mL. The intra- and inter-batch precision CV% were within the range of -1.3% to 7.7% and precision of intra- and inter-batch were below 15.0%. Furthermore, this method demonstrated low matrix effects and high recovery rates. The quetiapine plasma sample solution remained stable at room temperature for 25 hours and following 4 freeze-thaw cycles. The prepared samples remained stable in the autosampler (The temperature control of the autosampler was 5oC) for 185 hours and after four freeze-thaw cycles at -20oC and -70oC for 40 days. The present work effectively employed this approach to investigate the pharmacokinetics of orally administered quetiapine fumarate tablets in a cohort of healthy Chinese individuals, both in a fasting state and after a meal.


Assuntos
Análise Química do Sangue , População do Leste Asiático , Fumarato de Quetiapina , Espectrometria de Massas em Tandem , Humanos , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida/métodos , Fumarato de Quetiapina/administração & dosagem , Fumarato de Quetiapina/análise , Fumarato de Quetiapina/farmacocinética , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodos , Análise Química do Sangue/métodos , Voluntários Saudáveis
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