RESUMO
The prevalence of mansonelliasis was studied in the municipality of Tefé, state of Amazonas, Brazil. The prevalence (thick blood smear method) was 13.6% (147/1,078), higher in the Solimões River region (16.3%) than in the Tefé River region (6.3%). In the sampled communities in the Solimões River region, a higher density of cases was observed, as indicated by a kernel analysis (odds ratio 0.34; 95% confidence interval: 0.20-0.57). Males had a higher prevalence (χ2 = 31.292, p < 0.001) than women. Mansonella ozzardi prevalence was higher in retirees and farmers (28.9% and 27%, respectively). Prevalence also significantly increased with age (χ2 = -128.17, p < 0.001), with the highest numbers occurring in persons older than 67 years.
Assuntos
Insetos Vetores , Mansonella , Mansonelose/epidemiologia , Simuliidae , Adolescente , Adulto , Idoso , Animais , Brasil/epidemiologia , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , População Rural , Adulto JovemRESUMO
BACKGROUND: Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes. FINDINGS: RNA extracts of mosquito pools were tested by a RT-Hemi-Nested-PCR for detection of flaviviruses. One amplicon of 222 bp, compatible with dengue virus serotype 4, was obtained from a pool of Aedes aegypti. The nucleotide sequence of the amplicon indicated that the mosquitoes were infected with DENV-4 of genotype I. This virus of Asian origin has been described in Manaus in 2008 infecting acute febrile illness patients. CONCLUSION: This is the first report of dengue virus serotype 4 genotype I infecting Aedes aegypti in the Americas.
Assuntos
Aedes/virologia , Vírus da Dengue/classificação , Vírus da Dengue/isolamento & purificação , RNA Viral/genética , Animais , Brasil , Análise por Conglomerados , Vírus da Dengue/genética , Genótipo , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
BACKGROUND: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds. METHODS: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm. RESULTS: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee. CONCLUSIONS: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth.
Assuntos
Antibacterianos/farmacologia , Abelhas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Mel , Rutina/farmacologia , Animais , Antibacterianos/análise , Apiterapia , Cromatografia Líquida de Alta Pressão , Mel/análise , Fenóis/análise , Rutina/análiseRESUMO
INTRODUCTION: We investigated the prevalence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) infection in patients with hematological diseases from the western Amazon region of Brazil. METHODS: Samples from 306 patients were submitted for the molecular diagnosis of HTLV-1/2 infection by real time PCR (qPCR), with amplification, sequencing, and phylogenetic analysis of the long terminal repeat (LTR) region. RESULTS: A 29-year-old male carrier of sickle cell anemia with a history of multiple blood transfusions was diagnosed with the HTLV-2c subtype. CONCLUSIONS: This study describes the first known occurrence of HTLV-2c in the urban area of Brazil's western Amazon region.
Assuntos
Infecções por HTLV-I , Infecções por HTLV-II , Vírus Linfotrópico T Tipo 1 Humano , Adulto , Brasil/epidemiologia , Infecções por HTLV-I/diagnóstico , Infecções por HTLV-I/epidemiologia , Infecções por HTLV-II/diagnóstico , Infecções por HTLV-II/epidemiologia , Vírus Linfotrópico T Tipo 1 Humano/genética , Vírus Linfotrópico T Tipo 2 Humano/genética , Humanos , Masculino , FilogeniaRESUMO
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
Assuntos
Infecções por Adenoviridae/diagnóstico , Adenoviridae/genética , Nasofaringe/virologia , Infecções por Vírus Respiratório Sincicial/diagnóstico , Vírus Sincicial Respiratório Humano/genética , Infecções Respiratórias/diagnóstico , Doença Aguda , Adenoviridae/isolamento & purificação , Estudos de Casos e Controles , Criança , Humanos , Reação em Cadeia da Polimerase/métodos , Vírus Sincicial Respiratório Humano/isolamento & purificação , Infecções Respiratórias/virologia , Sensibilidade e EspecificidadeRESUMO
We report dengue virus type 4 (DENV-4) in Amazonas, Brazil. This virus was isolated from serum samples of 3 patients treated at a tropical medicine reference center in Manaus. All 3 cases were confirmed by serologic and molecular tests; 1 patient was co-infected with DENV-3 and DENV-4.
Assuntos
Vírus da Dengue/classificação , Dengue/epidemiologia , Dengue/virologia , Brasil/epidemiologia , Dengue/sangue , Vírus da Dengue/genética , Vírus da Dengue/isolamento & purificação , Humanos , RNA Viral/sangue , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
INTRODUCTION: Human T-lymphotropic virus type 1 and 2 (HTLV-1/2) is endemic in Brazil, but few studies have investigated the seroprevalence of HTLV and its subtypes among blood donors in the capital city Manaus, Amazonas State, Brazil. AIM: To estimate the seroprevalence of HTLV-1/2 and to identify circulating subtypes among blood donors in Manaus. MATERIALS AND METHODS: Blood donors (2001-2003) were screened for HTLV-1/2 antibodies by ELISA. Positive results were confirmed and subtyped by Western blot assays. Prevalence rates were calculated and compared with demographic data. RESULTS: Among the 87,402 individuals screened, 116 (0.13%) were seropositive for HTLV-1/2. A second sample (76/116) was collected and retested by HTLV-1/2 ELISA, of which only 41/76 were positive. Western blot confirmed HTLV infection in 24/41 retested blood donors [HTLV-1 (n=16), HTLV-2 (n=5) and HTLV-untypable (n=3)]. DISCUSSION: HTLV-1 and HTLV-2 are prevalent among blood donors in Manaus. However, additional studies are needed to comprehend the epidemiology of HTLV-1/2 in Amazonas not only to understand the pathophysiology of the disease providing adequate medical assistance, but also to reduce or block virus transmission.
Assuntos
Doadores de Sangue/estatística & dados numéricos , Infecções por HTLV-I/epidemiologia , Infecções por HTLV-II/epidemiologia , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Vírus Linfotrópico T Tipo 2 Humano/isolamento & purificação , Adolescente , Adulto , Western Blotting , Brasil/epidemiologia , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Feminino , Infecções por HTLV-I/virologia , Infecções por HTLV-II/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Soroepidemiológicos , Adulto JovemRESUMO
INTRODUCTION:: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS:: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS:: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS:: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.
Assuntos
Culicidae/virologia , Flavivirus/genética , Animais , Sequência de Bases , Brasil , Culicidae/classificação , Flavivirus/classificação , Filogenia , Reação em Cadeia da PolimeraseRESUMO
Abstract INTRODUCTION: We investigated the prevalence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) infection in patients with hematological diseases from the western Amazon region of Brazil. METHODS: Samples from 306 patients were submitted for the molecular diagnosis of HTLV-1/2 infection by real time PCR (qPCR), with amplification, sequencing, and phylogenetic analysis of the long terminal repeat (LTR) region. RESULTS: A 29-year-old male carrier of sickle cell anemia with a history of multiple blood transfusions was diagnosed with the HTLV-2c subtype. CONCLUSIONS: This study describes the first known occurrence of HTLV-2c in the urban area of Brazil's western Amazon region.
Assuntos
Humanos , Masculino , Gravidez , Adulto , Vírus Linfotrópico T Tipo 1 Humano/genética , Infecções por HTLV-I/diagnóstico , Infecções por HTLV-I/epidemiologia , Infecções por HTLV-II/diagnóstico , Infecções por HTLV-II/epidemiologia , Filogenia , Brasil/epidemiologia , Vírus Linfotrópico T Tipo 2 Humano/genéticaRESUMO
INTRODUCTION: This study assessed the prevalence of Mansonella ozzardi in riverine communities of the Tefé River, Amazonas, Brazil. METHODS: The prevalence of M. ozzardi was estimated by microscopic examination of thick blood smears. RESULTS: The M. ozzardi prevalence rate was 6.3% (19/300). Filarial infection was found in 8 of the 11 communities surveyed, with prevalence rates varying from 2.5% to 22.2%. CONCLUSIONS: Tefé is a region of oil and natural gas exploration, in which there is a high turnover of workers. Migration patterns may facilitate the dissemination of mansonelliasis to other regions.
Assuntos
Mansonella/isolamento & purificação , Mansonelose/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Brasil/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Mansonella/classificação , Mansonelose/diagnóstico , Pessoa de Meia-Idade , Prevalência , Rios , População Rural , Adulto JovemRESUMO
INTRODUCTION: Hepatitis B virus (HBV) infection is one of the most serious public health problems in the world. In Brazil, HBV endemicity is heterogeneous, with the highest disease prevalence in the North region. METHODS: A total of 180 samples were analyzed and subjected to polymerase chain reaction (PCR) and semi-nested PCR of the HBV S-gene, with the aim of determining the prevalence of HBV-DNA (deoxyribonucleic acid) in indigenous groups inhabiting the areas near the Curuçá and Itaquaí Rivers in the Javari Valley, State of Amazonas, Brazil. RESULTS: The prevalence of the HBV-DNA S-gene was 51.1% (92/180). The analysis found 18 of 49 (36.7%) samples from the Marubo tribe, 68 of 125 (54.4%) from the Kanamary, and 6 of 6 (100%) from other ethnic groups to be PCR positive. There was no statistically significant difference in gender at 5% (p=0.889). Indigenous people with positive PCR for HBV-DNA had a lower median age (p<0.001) of 23 years. There was no statistical difference found in relation to sources of contamination or clinical aspects with the PCR results, except for fever (p<0.001). The high prevalence of HBV-DNA of 75% (15/20) in pregnant women (p=0.009) demonstrates an association with vertical transmission. CONCLUSIONS: The results confirm the high prevalence of HBV-DNA in the Javari Valley, making it important to devise strategies for control and more effective prevention in combating the spread of HBV.
Assuntos
DNA Viral/genética , Vírus da Hepatite B/genética , Hepatite B/epidemiologia , Indígenas Sul-Americanos/estatística & dados numéricos , Adolescente , Adulto , Idoso , Brasil/epidemiologia , Criança , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Gravidez , Prevalência , Adulto JovemRESUMO
Abstract INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.
Assuntos
Animais , Flavivirus/genética , Culicidae/virologia , Filogenia , Brasil , Sequência de Bases , Reação em Cadeia da Polimerase , Flavivirus/classificação , Culicidae/classificaçãoRESUMO
INTRODUCTION: Dengue is an arbovirus that continues to cause serious public health problems in tropical and sub-tropical regions of the planet. METHODS: In this study, blood samples from children were investigated by RT-PCR, aimed at identifying dengue virus serotypes in Manaus, AM, in 2008 in this infant population. RESULTS: DENV-3 was the only serotype identified. CONCLUSIONS: In this study, 83% of children examined were negative for dengue by RT-PCR, suggesting the occurrence of other febrile illnesses that need to be determined.
Assuntos
Vírus da Dengue/classificação , Dengue/virologia , RNA Viral/sangue , Brasil/epidemiologia , Criança , Pré-Escolar , Dengue/epidemiologia , Vírus da Dengue/genética , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SorotipagemRESUMO
The aim of this study was to detect and type dengue viruses in the vector Aedes aegypti. Between December 2005 and December 2006, 8,984 mosquitoes were collected in 46 districts of the city of Manaus, covering all of the geographical zones of the city. Of these, 819 were Aedes aegypti (414 females and 405 males). The females of Aedes aegypti were grouped in pools of 1 to 10 mosquitoes, thus totaling 138 pools, of which 111 pools were positive for DENV 3 and a single pool was positive for two serotypes (DENV 1 and DENV 3). The prevalence of Aedes aegypti infected with DENV 3 in the city of Manaus was 53%. The zonal prevalence was 70% in the western central zone, 60% in the southern zone, 53% in the western zone, 47% in the southern central zone, 30% in the northern zone and 23% in the eastern zone. Monitoring of virus circulation among mosquitoes by means of the reverse transcription polymerase chain reaction technique enables prior knowledge of the levels of virus spread in given areas, thus contributing towards determining the localities where prevention and control measures should be applied.
Assuntos
Aedes/virologia , Vírus da Dengue/classificação , Insetos Vetores/virologia , RNA Viral/análise , Animais , Brasil , Dengue/transmissão , Dengue/virologia , Vírus da Dengue/isolamento & purificação , Feminino , Masculino , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SorotipagemRESUMO
Introduction: This study assessed the prevalence of Mansonella ozzardi in riverine communities of the Tefé River, Amazonas, Brazil. Methods: The prevalence of M. ozzardi was estimated by microscopic examination of thick blood smears. Results: The M. ozzardi prevalence rate was 6.3% (19/300). Filarial infection was found in 8 of the 11 communities surveyed, with prevalence rates varying from 2.5% to 22.2%. Conclusions: Tefé is a region of oil and natural gas exploration, in which there is a high turnover of workers. Migration patterns may facilitate the dissemination of mansonelliasis to other regions. .
Assuntos
Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Mansonella/isolamento & purificação , Mansonelose/epidemiologia , Brasil/epidemiologia , Mansonella/classificação , Mansonelose/diagnóstico , Prevalência , Rios , População RuralRESUMO
The prevalence of mansonelliasis was studied in the municipality of Tefé, state of Amazonas, Brazil. The prevalence (thick blood smear method) was 13.6% (147/1,078), higher in the Solimões River region (16.3%) than in the Tefé River region (6.3%). In the sampled communities in the Solimões River region, a higher density of cases was observed, as indicated by a kernel analysis (odds ratio 0.34; 95% confidence interval: 0.20-0.57). Males had a higher prevalence (χ2 = 31.292, p < 0.001) than women. Mansonella ozzardi prevalence was higher in retirees and farmers (28.9% and 27%, respectively). Prevalence also significantly increased with age (χ2 = -128.17, p < 0.001), with the highest numbers occurring in persons older than 67 years.
Assuntos
Adolescente , Adulto , Idoso , Animais , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Insetos Vetores , Mansonella , Mansonelose/epidemiologia , Simuliidae , Brasil/epidemiologia , Prevalência , População RuralRESUMO
Objetivo: descrever a ocorrência de genótipos de rotavírus A em crianças na idade de 1 a 36 meses no município de Manaus, Estado do Amazonas, Brasil. Métodos: estudo descritivo, com resultados de análises laboratoriais de fezes diarréicas de crianças de 1 a 36 meses, atendidas em prontos-socorros infantis de Manaus-AM no período de junho/2004 a dezembro/2006; foram empregados os métodos de eletroforese de ácido ribonucleico em gel de poliacrilamida, transcriptase reversa e reação em cadeia da polimerase. Resultados: constatou-se 25 por cento de positividade para rotavírus A 30,7 por cento e 44,7 por cento para genótipos G e P, respectivamente , com predominância dos genótipos G2 (17 por cento) e P[8] (66,7 por cento); das amostras positivas para os genótipos G e P, 38,3 por cento e 11,7 por cento, respectivamente, não apresentaram características para qualquer dos genótipos G e P pesquisados. Conclusão: a elevada proporção de ausência de características dos genótipos G e P pesquisados sugere a circulação de genótipos diferentes desses.
Objective: verify rotavirus A genotype occurrence in children aged 1 to 36 months in Manaus-AM. Methods: descriptive and retrospective study conducted from June/2004 to December/2006, through laboratory analysis of diarrheal stools of children aged 1 to 36 months cared for at a Child Emergency service in anaus/Amazonas. Results: 25 per cent rotavirus A positivity was found using Polyacrylamide Gel Electrophoresis of Ribonucleic Acid. Reverse Transcriptase followed by Polymerase Chain Reaction were applied to positive samples and revealed 30.7 per cent G genotypes and 44.7 per cent P genotypes, with predominance of G2 (17 per cent) and P[8] (66.7 per cent) genotypes. 38.3 per cent and 11.7 per cent of the G and P genotype positive samples, respectively, showed no features of any of the G and P genotypes surveyed. Conclusion: 38.3 per cent and 11.7 per cent G and P genotypes, respectively, showed no characteristics of the genotypes studied, suggesting that genotypes different to these are in circulation.
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Epidemiologia Descritiva , Gastroenterite/epidemiologia , Infecções por Rotavirus/epidemiologiaRESUMO
INTRODUCTION: Hepatitis B virus (HBV) infection is one of the most serious public health problems in the world. In Brazil, HBV endemicity is heterogeneous, with the highest disease prevalence in the North region. METHODS: A total of 180 samples were analyzed and subjected to polymerase chain reaction (PCR) and semi-nested PCR of the HBV S-gene, with the aim of determining the prevalence of HBV-DNA (deoxyribonucleic acid) in indigenous groups inhabiting the areas near the Curuçá and Itaquaí Rivers in the Javari Valley, State of Amazonas, Brazil. RESULTS: The prevalence of the HBV-DNA S-gene was 51.1% (92/180). The analysis found 18 of 49 (36.7%) samples from the Marubo tribe, 68 of 125 (54.4%) from the Kanamary, and 6 of 6 (100%) from other ethnic groups to be PCR positive. There was no statistically significant difference in gender at 5% (p=0.889). Indigenous people with positive PCR for HBV-DNA had a lower median age (p<0.001) of 23 years. There was no statistical difference found in relation to sources of contamination or clinical aspects with the PCR results, except for fever (p<0.001). The high prevalence of HBV-DNA of 75% (15/20) in pregnant women (p=0.009) demonstrates an association with vertical transmission. CONCLUSIONS: The results confirm the high prevalence of HBV-DNA in the Javari Valley, making it important to devise strategies for control and more effective prevention in combating the spread of HBV.
INTRODUÇÃO: A infecção pelo vírus da hepatite B (VHB) é um dos mais sérios problemas de saúde pública do mundo. No Brasil, a endemicidade do VHB é heterogênea, sendo a doença mais prevalente na região norte do país. MÉTODOS: Neste estudo, foram investigadas 180 amostras de sangue por meio da técnica da reação em cadeia da polimerase (PCR) e PCR semi-nested para o vírus da hepatite B, gene S, com o intuito de determinar a prevalência do DNA (ácido desoxirribonucléico) do vírus da hepatite B em povos de etnias indígenas habitantes dos Rios Curuçá e Itaquaí no Vale do Javari, Estado do Amazonas, Brasil. RESULTADOS: A prevalência encontrada para o DNA-VHB gene S foi de 51,1% (92/180). Entre as amostras positivas 18/49 (36,7%) pertenciam à etnia Marubo, 68/125 (54,4%) à Kanamary e 6/6 (100%) a outras etnias. Não houve diferença significante ao nível de 5% em relação ao gênero (p=0,889). Os indígenas com PCR positiva para DNA-VHB apresentaram mediana de idade menor de 23 anos (p<0,001). Não foi constatado nenhuma diferença estatística em relação às fontes de contágio e o resultado da PCR, como também aos aspectos clínicos, com exceção da febre (p<0,001). A alta prevalência do DNA-VHB de 75% (15/20) em gestantes (p=0,009) demonstra associação com a transmissão vertical. CONCLUSÕES: Os resultados comprovam a alta prevalência do DNA-VHB no Vale do Javari, tornando-se importante traçar estratégias de controle e prevenção mais eficazes no combate à disseminação do VHB.
Assuntos
Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Gravidez , Adulto Jovem , DNA Viral/genética , Vírus da Hepatite B/genética , Hepatite B/epidemiologia , Indígenas Sul-Americanos/estatística & dados numéricos , Brasil/epidemiologia , Estudos Transversais , Reação em Cadeia da Polimerase , PrevalênciaRESUMO
INTRODUCÃO: A dengue é uma arbovirose que vem causando sérios problemas de saúde pública, em regiões tropicais e subtropicais do planeta. MÉTODOS: Neste estudo, foram investigadas amostras de sangue de crianças, através da RT-PCR, com o intuito de se identificar sorotipos do vírus dengue nessa população infantil, em Manaus/AM, durante o ano de 2008. RESULTADOS: O DENV-3 foi o único sorotipo viral identificado. CONCLUSÕES: No presente estudo, 83 por cento das crianças analisadas apresentaram resultado negativo para dengue através do RT-PCR sugerindo a ocorrência de outras doenças febris que necessitam ser esclarecidas.
INTRODUCTION: Dengue is an arbovirus that continues to cause serious public health problems in tropical and sub-tropical regions of the planet. METHODS: In this study, blood samples from children were investigated by RT-PCR, aimed at identifying dengue virus serotypes in Manaus, AM, in 2008 in this infant population. RESULTS: DENV-3 was the only serotype identified. CONCLUSIONS: In this study, 83 percent of children examined were negative for dengue by RT-PCR, suggesting the occurrence of other febrile illnesses that need to be determined.
Assuntos
Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Vírus da Dengue/classificação , Dengue/virologia , RNA Viral/sangue , Brasil/epidemiologia , Vírus da Dengue/genética , Dengue/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SorotipagemRESUMO
Os rotavírus do grupo A, são frequentemente associados com gastroenterites em mamíferos e aves. O objetivo deste trabalho foi detectar a presença de rotavírus em fezes de cães sintomáticos e assintomáticos para diarréia aguda. Foram coletadas 32 amostras de fezes de cães. Todas as amostras foram submetidas à extração do RNA viral seguida da Eletroforese em Gel de Poliacrilamida (EGPA), onde se identificou apenas 1 (um) caso de infecção por rotavírus, em amostra assintomática. A análise do eletroferótipo mostrou um perfil 4:2:3:2 longo, e a homologia dos eletroferótipos de rotavírus humano e canino foi muito alta, sugerindo uma possível infecção interespécie.
The group A rotaviruses are frequently associated with gastroenteritis in mammals and birds. The objective of this study was to detect the presence of rotavirus in feces of symptomatic and asymptomatic dogs for acute diarrhea. 32 samples of dog feces were collected. All the samples were submitted to viral RNA extraction followed by electrophoresis in poliacrylamide gels (PAGE), where only one case of rotavirus infection in one asymptomatic sample was found. The electrophoretic analysis showed 4:2:3:2 long profile, and the homology between human and dog rotaviruses was very high. This suggests a possible interspecies infection.