1.
Electrophoresis
; 22(6): 1021-3, 2001 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11358123
RESUMO
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.