1.
J Inorg Biochem
; 233: 111869, 2022 08.
Artigo
em Inglês
| MEDLINE
| ID: mdl-35653820
RESUMO
Metallo-ß-lactamases (MBLs) are enzymes that are capable of hydrolyzing most ß-lactam antibiotics and all clinically relevant carbapenems. We developed a library of reversible fluorescent turn-on probes that are designed to directly bind to the dizinc active site of these enzymes and can be used to study their dynamic metalation state and enzyme-inhibitor interactions. Structure-function relationships with regards to inhibitory strength and fluorescence turn-on response were evaluated for three representative MBLs.