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1.
Med Vet Entomol ; 29(3): 263-75, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25761054

RESUMO

In 2011, Culicoides (Diptera: Ceratopogonidae) were collected at 16 locations covering four regions of Belgium with Onderstepoort Veterinary Institute (OVI) traps and at two locations with Rothamsted suction traps (RSTs). Quantification of the collections and morphological identification showed important variations in abundance and species diversity between individual collection sites, even for sites located in the same region. However, consistently higher numbers of Culicoides midges were collected at some sites compared with others. When species abundance and diversity were analysed at regional level, between-site variation disappeared. Overall, species belonging to the subgenus Avaritia together with Culicoides pulicaris (subgenus Culicoides) were the most abundant, accounting for 80% and 96% of all midges collected with RSTs and OVI traps, respectively. Culicoides were present during most of the year, with Culicoides obsoletus complex midges found from 9 February until 27 December. Real-time reverse-transcription polymerase chain reaction screening for Schmallenberg virus in the heads of collected midges resulted in the first detection of the virus in August 2011 and identified C. obsoletus complex, Culicoides chiopterus and Culicoides dewulfi midges as putative vector species. At Libramont in the south of Belgium, no positive pools were identified.


Assuntos
Infecções por Bunyaviridae/epidemiologia , Ceratopogonidae/fisiologia , Insetos Vetores/fisiologia , Orthobunyavirus/fisiologia , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/virologia , Ceratopogonidae/virologia , Insetos Vetores/virologia , Reação em Cadeia da Polimerase , Densidade Demográfica , Especificidade da Espécie
2.
J Virol ; 86(13): 7235-40, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22532685

RESUMO

We have previously shown that the porcine alphaherpesvirus pseudorabies virus (PRV) efficiently interferes with phosphorylation of the eukaryotic translation initiation factor eIF2α. Inhibition of phosphorylation of eIF2α has been reported earlier for the closely related alphaherpesvirus herpes simplex virus 1 (HSV-1) through its ICP34.5 and US11 proteins. PRV, however, does not encode an ICP34.5 or US11 orthologue. Assays using cycloheximide, UV-inactivated PRV, or phosphonoacetic acid (PAA) showed that de novo expression of one or more (immediate) early viral protein(s) is required for interference with eIF2α phosphorylation. In line with this, a time course assay showed that eIF2α phosphorylation was abolished within 2 h after PRV inoculation. PRV encodes only one immediate-early protein, IE180, the orthologue of HSV-1 ICP4. As reported earlier, a combinational treatment of cells with cycloheximide and actinomycin D allowed expression of IE180 without detectable expression of the US3 early protein in PRV-infected cells. This led to a substantial reduction in eIF2α phosphorylation levels, indicative for an involvement of IE180. In support of this, transfection of IE180 also potently reduced eIF2α phosphorylation. IE180-mediated interference with eIF2α phosphorylation was not cell type dependent, as it occurred both in rat neuronal 50B11 cells and in swine testicle cells. Inhibition of the cellular phosphatase PP1 impaired PRV-mediated interference with eIF2α phosphorylation, indicating that PP1 is involved in this process. In conclusion, the immediate-early IE180 protein of PRV has the previously uncharacterized ability to suppress phosphorylation levels of the eukaryotic translation initiation factor eIF2α.


Assuntos
Fator de Iniciação 2 em Eucariotos/metabolismo , Herpesvirus Suídeo 1/patogenicidade , Biossíntese de Proteínas , Proteínas Virais/metabolismo , Fatores de Virulência/metabolismo , Animais , Linhagem Celular , Fosforilação , Processamento de Proteína Pós-Traducional , Ratos , Suínos
3.
Sci Rep ; 9(1): 20076, 2019 12 27.
Artigo em Inglês | MEDLINE | ID: mdl-31882819

RESUMO

Lumpy skin disease (LSD) is a devastating disease of cattle characterized by fever, nodules on the skin, lymphadenopathy and milk drop. Several haematophagous arthropod species like dipterans and ticks are suspected to play a role in the transmission of LSDV. Few conclusive data are however available on the importance of biting flies and horseflies as potential vectors in LSDV transmission. Therefore an in vivo transmission study was carried out to investigate possible LSDV transmission by Stomoxys calcitrans biting flies and Haematopota spp. horseflies from experimentally infected viraemic donor bulls to acceptor bulls. LSDV transmission by Stomoxys calcitrans was evidenced in 3 independent experiments, LSDV transmission by Haematopota spp. was shown in one experiment. Evidence of LSD was supported by induction of nodules and virus detection in the blood of acceptor animals. Our results are supportive for a mechanical transmission of the virus by these vectors.


Assuntos
Dípteros/virologia , Mordeduras e Picadas de Insetos , Insetos Vetores , Doença Nodular Cutânea/transmissão , Vírus da Doença Nodular Cutânea/patogenicidade , Animais , Bovinos , DNA Viral/genética , Doença Nodular Cutânea/virologia , Vírus da Doença Nodular Cutânea/genética
4.
Transbound Emerg Dis ; 64(4): 1015-1019, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28474485

RESUMO

A seroprevalence study carried out between June and September 2016 in the Belgian sheep population showed a significant increase in overall (from 25% to 62%) and between-herd (from 60% to 96%) seroprevalence against Schmallenberg virus (SBV) during this period, indicating the most extensive recirculation of SBV since its original emergence in 2011. SBV recirculation was confirmed by the detection of SBV RNA-positive Culicoides obsoletus complex midges collected in the region of Antwerp in August 2016, reaching a minimum infection rate of 3%. The recirculation of SBV in the largely unprotected ruminant population during summer 2016 will likely cause an increase in the number of arthrogryposis-hydranencephaly cases in newborn ruminants during the coming months.


Assuntos
Artrogripose/veterinária , Infecções por Bunyaviridae/veterinária , Hidranencefalia/veterinária , Orthobunyavirus/isolamento & purificação , Doenças dos Ovinos/virologia , Animais , Artrogripose/epidemiologia , Artrogripose/virologia , Bélgica/epidemiologia , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Ceratopogonidae/virologia , Hidranencefalia/epidemiologia , Hidranencefalia/virologia , Orthobunyavirus/genética , RNA Viral , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia
5.
Transbound Emerg Dis ; 64(5): 1641-1642, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27485019

RESUMO

In spring 2016, three years after the last reported outbreak of Schmallenberg virus (SBV) in Belgium, an abortion was notified in a two year old Holstein heifer that previously had not been vaccinated against SBV. The autopsy of the eight-month-old malformed foetus revealed hydrocephalus, torticollis and arthrogryposis. Foetal brain tissue and blood were found to be SBV-positive by RT-PCR and ELISA tests, respectively. Evidencing the circulation of SBV in Belgium in the autumn 2015 is important to anticipate future outbreaks and advise veterinarians about the risks associated with calving, as more bovine foetuses might have been infected.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/virologia , Orthobunyavirus , Aborto Animal/virologia , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Bovinos , Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Gravidez , Complicações Infecciosas na Gravidez/veterinária , Complicações Infecciosas na Gravidez/virologia
6.
Transbound Emerg Dis ; 64(1): 305-308, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25891033

RESUMO

Schmallenberg virus (SBV) is an emerging Orthobunyavirus affecting European domestic ruminants. In this study, three groups of ewes (n = 3) were inoculated with 1 ml of an SBV infectious serum, via the subcutaneous (SC), intradermal (ID) or intranasal (IN) route. The ewes were monitored for 10 days and no clinical signs were reported. IN inoculation failed to generate any detectable RNAemia. SC and ID inoculation induced typical SBV RNAemia and seroconversion upon day 6 post-inoculation in 3/3 and 2/3 sheep, respectively. In all the animals that showed RNAemia, the viral genome could be detected in spleen and mesenteric lymph nodes. Both the SC and ID routes seem suitable to properly reproduce field conditions, as comparable observations were reported regarding RNAemia, seroconversion and viral genome detection in organs.


Assuntos
Infecções por Bunyaviridae/veterinária , Orthobunyavirus/fisiologia , Doenças dos Ovinos/prevenção & controle , Vacinação/veterinária , Administração Intranasal/veterinária , Animais , Infecções por Bunyaviridae/prevenção & controle , Infecções por Bunyaviridae/virologia , Feminino , Injeções Intradérmicas/veterinária , Injeções Subcutâneas/veterinária , Linfonodos/virologia , Ovinos , Doenças dos Ovinos/virologia , Baço/virologia
7.
Vet Microbiol ; 113(3-4): 251-5, 2006 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-16359830

RESUMO

A key aspect in the life cycle of alphaherpesviruses is their neurotropic behaviour. Sensory neurons of the trigeminal ganglion (TG) are important target cells for many alphaherpesviruses (including herpes simplex virus 1, pseudorabies virus (PRV), bovine herpesvirus 1) and constitute major sites for latent infections. The aim of this study was to develop an in vitro model that simulates the in vivo infection pattern of TG neurons by alphaherpesviruses. To this end, we developed a homologous in vitro two-chamber model using PRV and porcine TG neurons. TG of 4- to 6-week-old piglets were dissociated and cultured in the inner chamber of the in vitro model, which is separated from the outer chamber by a medium- and virus-impermeable silicon barrier. Outgrowth of axons from neuronal cell bodies in the inner chamber through the silicon barrier into the outer chamber could be observed after 2-3 weeks of cultivation. Subsequent addition of PRV to the outer chamber resulted in exclusive infection of the TG neurons by transport of virus through the axons, subsequently giving rise to productively infected TG neurons that transmitted virus to contacting neurons and non-neuronal cells in the inner chamber. Thus, we established a homologous in vitro model that mimics the natural route of alphaherpesvirus infection of TG neurons that can be used to study interactions between these viruses and this pathogenetically very important cell type.


Assuntos
Alphaherpesvirinae/patogenicidade , Infecções por Herpesviridae/veterinária , Neurônios/virologia , Gânglio Trigeminal/virologia , Alphaherpesvirinae/ultraestrutura , Animais , Infecções por Herpesviridae/virologia , Técnicas In Vitro , Microscopia Confocal/veterinária , Neurônios/ultraestrutura , Suínos , Gânglio Trigeminal/ultraestrutura
8.
Transbound Emerg Dis ; 62(5): e80-4, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24330658

RESUMO

Schmallenberg virus (SBV), which emerged in Northwestern Europe in 2011, is an arthropod-borne virus affecting primarily ruminants. Based on the results of two cross-sectional studies conducted in the Belgian ruminant population during winter 2011-2012, we concluded that at the end of 2011, almost the whole population had already been infected by SBV. A second cross-sectional serological study was conducted in the Belgian cattle population during winter 2012-2013 to examine the situation after the 2012 transmission period and to analyse the change in immunity after 1 year. A total of 7130 blood samples collected between 1st January and 28 February 2013 in 188 herds were tested for the presence of SBV-specific antibodies. All sampled herds tested positive and within-herd seroprevalence was estimated at 65.66% (95% CI: 62.28-69.04). A statistically significant decrease was observed between the beginning and the end of 2012. On the other hand, age-cohort-specific seroprevalence stayed stable from 1 year to the other. During winter 2012-2013, calves between 6 and 12 months had a seroprevalence of 20.59% (95% CI: 15.34-25.83), which seems to be an indication that SBV was still circulating at least in some parts of Belgium during summer-early autumn 2012. Results showed that the level of immunity against SBV of the animals infected has not decreased and remained high after 1 year and that the spread of the virus has slowed down considerably during 2012. This study also indicated that in the coming years, there are likely to be age cohorts of unprotected animals.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/epidemiologia , Orthobunyavirus/isolamento & purificação , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/sangue , Infecções por Bunyaviridae/epidemiologia , Bovinos , Doenças dos Bovinos/sangue , Estudos Transversais , Seguimentos , Orthobunyavirus/imunologia , Fatores de Risco , Estações do Ano , Estudos Soroepidemiológicos
9.
Transbound Emerg Dis ; 61(5): 425-31, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23305427

RESUMO

A serological survey to detect Schmallenberg virus (SBV)-specific antibodies by ELISA was organized in the Belgian sheep population to study the seroprevalence at the end of the epidemic. One thousand eighty-two sheep samples which were collected from 83 herds all over Belgium between November 2011 and April 2012 were tested. The overall within-herd seroprevalence and the intraclass correlation coefficient were estimated at 84.31% (95% CI: 84.19-84.43) and 0.34, respectively. The overall between-herd seroprevalence was 98.03% (95% CI: 97.86-98.18). A spatial cluster analysis identified a cluster of six farms with significantly lower within-herd seroprevalence in the south of Belgium compared with the rest of the population (P = 0.04). It was shown that seroprevalence was associated to flock density and that the latter explained the presence of the spatial cluster. Additionally, 142 goat samples from eight different herds were tested for SBV-specific antibodies. The within-herd seroprevalence in goats was estimated at 40.68% (95% CI: 23.57-60.4%). The results of the current study provided evidence that almost every Belgian sheep herd has been in contact with SBV during 2011 and should be taken into consideration as part of comprehensive SBV surveillance and control strategies.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças das Cabras/virologia , Orthobunyavirus/isolamento & purificação , Doenças dos Ovinos/virologia , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/epidemiologia , Epidemias/veterinária , Doenças das Cabras/epidemiologia , Cabras , Orthobunyavirus/classificação , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia
10.
Vet J ; 201(3): 419-22, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24954869

RESUMO

Schmallenberg virus (SBV) is an orthobunyavirus affecting European domestic ruminants. In this study, the dose-dependent effect of experimental infection of sheep with SBV was evaluated. Four groups of three ewes were each inoculated subcutaneously with 1 mL of successive 10-fold dilutions of an SBV infectious serum. The ewes were monitored for 10 days, but no clinical signs were observed. The number of productively infected animals within each group, as evidenced by viraemia, seroconversion and viral RNA in the organs, depended on the inoculated dose, indicating that a critical dose has to be administered to obtain a homogeneous response in infected animals under experimental conditions. In the productively infected animals, no statistical differences between the different inoculation doses were found in the duration or quantity of viral RNA circulating in blood, nor in the amount of viral RNA present in virus positive lymphoid organs.


Assuntos
Infecções por Bunyaviridae/veterinária , Orthobunyavirus/fisiologia , Doenças dos Ovinos/virologia , Viremia/veterinária , Animais , Infecções por Bunyaviridae/virologia , Bovinos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Orthobunyavirus/genética , Reação em Cadeia da Polimerase/veterinária , Ovinos , Viremia/virologia
11.
Transbound Emerg Dis ; 60(1): 4-8, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23206240

RESUMO

A cross-sectional survey was conducted in the Belgian cattle population after the first period of infection of the emerging Schmallenberg virus. A total number of 11 635 cattle from 422 herds sampled between 2 January and 7 March 2012 were tested for the presence of Schmallenberg-specific antibodies using an ELISA kit. Between-herd seroprevalence in cattle was estimated at 99.76% (95% CI: 98.34-99.97) and within-herd seroprevalence at 86.3% (95% CI: 84.75-87.71). An Intraclass Correlation Coefficient of 0.3 (P < 0.001) was found, indicating that the correlation between two animals within a herd with respect to their serological status was high. Those results corroborate the conclusion that the Schmallenberg virus was widespread in Belgium during winter 2011. Seroprevalence was shown to be statistically associated to the animal's age (P < 0.0001): with 64.9% (95% CI: 61.34-68.3) estimated for the 6-12 months of age, 86.79% (95% CI: 84.43-88.85) for the 12-24 months of age and 94.4% (95% CI: 93.14-95.44) for the animals older than 24 months. Based on the results of the described serological survey, we can conclude that after the first Schmallenberg virus episode, almost every Belgian cattle has already been in contact with the virus. In consequence, the vast majority of the host animals should have developed post infection protective immunity against the virus.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/virologia , Orthobunyavirus/isolamento & purificação , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/sangue , Infecções por Bunyaviridae/diagnóstico , Infecções por Bunyaviridae/epidemiologia , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Feminino , Estações do Ano , Estudos Soroepidemiológicos , Testes Sorológicos
12.
Theriogenology ; 78(7): 1527-37, 2012 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-22980086

RESUMO

Preventing congenital infection is important for the control of porcine reproductive and respiratory syndrome (PRRS). Recently, in our laboratory, an inactivated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine has been developed. Promising results in young pigs encouraged us to test the vaccine potency to prevent congenital infection. In the present study, the performance of this experimental inactivated vaccine was investigated in pregnant gilts. An advanced protocol was used to test the PRRSV vaccine efficacy. This protocol is based on recent insights in the pathogenesis of congenital PRRSV infections. Three gilts were vaccinated with an experimental PRRSV 07V63 inactivated vaccine at 27, 55, and 83 days of gestation. Three unvaccinated gilts were included as controls. At 90 days of gestation, all animals were intranasally inoculated with 10(5) tissue culture infectious dose 50 (TCID(50)) of PRRSV 07V63. Twenty days postchallenge animals were euthanized and sampled. The vaccinated gilts quickly developed virus neutralizing (VN) antibodies starting from 3 to 7 days postchallenge (1.0 to 5.0 log2). In contrast, the unvaccinated gilts remained negative for VN antibodies after challenge. The vaccinated gilts had shorter viremia than the control gilts. Gross pathology (mummification) was observed in 8% of the fetuses from vaccinated gilts and in 15% of the fetuses from unvaccinated gilts. The number of fetuses with severe microscopic lesions in the fetal implantation sites (a focal detachment of the trophoblast from the uterine epithelium; a focal, multifocal, or full degeneration of the fetal placenta) was lower in the vaccinated (19%) versus unvaccinated (45%) gilts (P < 0.05). The number of PRRS-positive cells in the fetal placentae was higher in unvaccinated versus vaccinated gilts (P < 0.05). In contrast, the number of PRRS-positive cells in the myometrium/endometrium was higher in vaccinated versus unvaccinated gilts (P < 0.05). Fifty-seven percent of the fetuses from the vaccinated gilts and 75% of the fetuses from the unvaccinated gilts were PRRSV-positive. In conclusion, implementation of the novel experimental inactivated PRRSV vaccine primed the VN antibody response and slightly reduced the duration of viremia in gilts. It also reduced the number of virus-positive fetuses and improved the fetal survival, but was not able to fully prevent congenital PRRSV infection. The reduction of fetal infection and pathology is most probably attributable to the vaccine-mediated decrease of PRRSV transfer from the endometrium to the fetal placenta.


Assuntos
Síndrome Respiratória e Reprodutiva Suína/congênito , Síndrome Respiratória e Reprodutiva Suína/prevenção & controle , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Complicações Infecciosas na Gravidez/veterinária , Vacinas de Produtos Inativados , Vacinas Virais , Animais , Anticorpos Antivirais/sangue , Feminino , Doenças Fetais/virologia , Feto/virologia , Idade Gestacional , Placenta/virologia , Gravidez , Complicações Infecciosas na Gravidez/prevenção & controle , Complicações Infecciosas na Gravidez/virologia , Sus scrofa , Suínos , Vacinação/veterinária , Viremia/prevenção & controle , Viremia/veterinária
13.
Transbound Emerg Dis ; 59(6): 471-5, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23025501

RESUMO

To identify possible vectors of Schmallenberg virus (SBV), we tested pools containing heads of biting midges (Culicoides) that were caught during the summer and early autumn of 2011 at several places in Belgium by real-time RT-PCR. Pools of heads originating from following species: C. obsoletus complex, C. dewulfi and C. chiopterus were found positive, strongly indicating that these species are relevant vectors for SBV.


Assuntos
Ceratopogonidae/virologia , Insetos Vetores/virologia , Orthobunyavirus/isolamento & purificação , Animais , Bélgica/epidemiologia , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/transmissão , Infecções por Bunyaviridae/veterinária , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Estações do Ano
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