RESUMO
Phytochemical investigation on the stems of Kadsura renchangiana led to the isolation of two new sesquiterpenoids, renchangianins F and G (1 and 2). Their structures were elucidated by spectroscopic methods, including 2D NMR techniques. The in vitro cytotoxic activities of the isolates were studied against HepG2, A549, and LN229 cancer cell lines.
Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Kadsura/química , Sesquiterpenos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Caules de Planta/química , Sesquiterpenos/química , Sesquiterpenos/farmacologiaRESUMO
A new isocoumarin, chamerilactone A(1) was isolated from the ethanol extract of Chamerion angustifolium with normal phase silica column chromatography, Sephadex LH-20, MCI CHP-20 and semi- preparative HPLC methods. Its structure and stereochemistry were elucidated by spectroscopic methods, including 2D-NMR and optical rotation techniques.
Assuntos
Isocumarinas/isolamento & purificação , Onagraceae/química , Cromatografia Líquida de Alta Pressão , Estrutura MolecularRESUMO
Phytochemical investigation on the seeds of Herpetospermum caudigerum afforded two new coumarin glycosides A and B (1 and 2), together with a known compound herpetolide A (3). Their structures were determined by spectroscopic methods, including 2D NMR techniques. Compound 3 exhibited cytotoxicity against HepG2 cells with IC50 value of 6.47 µg/ml. Compound 2 showed anti-hepatitis B virus activity with HBsAg secretion by 33.1% at 200 µg/ml.
Assuntos
Antivirais/isolamento & purificação , Cumarínicos/isolamento & purificação , Cucurbitaceae/química , Glicosídeos/isolamento & purificação , Antivirais/química , Antivirais/farmacologia , Cumarínicos/química , Cumarínicos/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Células Hep G2 , Vírus da Hepatite B/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Sementes/químicaRESUMO
A new dibenzocyclooctadiene lignan, renchangianin E (1) was isolated from the stems of Kadsura renchangiana. Its structure and stereochemistry were elucidated by spectroscopic methods, including 2D-NMR techniques.
Assuntos
Ciclo-Octanos/química , Kadsura/química , Lignanas/química , Espectroscopia de Ressonância Magnética , Estrutura MolecularRESUMO
A new isoflavanone compound, ficustikounone A (1), together with 22 known flavones, was isolated from Ficus tikoua Bur. The structures of these isolates were determined by UV, ECD, HRESIMS, 1D and 2D spectral analyses.
Assuntos
Ficus/química , Isoflavonas/isolamento & purificação , Células A549 , Linhagem Celular Tumoral , Flavonas , Células Hep G2 , Humanos , Isoflavonas/química , Estrutura Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Plantas Medicinais/químicaRESUMO
The aerial parts of Epilobium plants are widely used as folk medicine and food around the world. The present study was aimed to investigate the antioxidant activities and active chemical constituents from Epilobium angustifolium L. The results revealed that the EtOAc extract, rich in phenolic compounds and flavonoids (16.81 ± 0.67 g GAE/100 g extract and 4.95 ± 0.21 g QE/100 g extract, respectively), possessed significantly antioxidant activities in reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity and highly in inhibiting lipid peroxidation activity. Simultaneously, active fractions F to H from EtOAc extracts showing potent in vitro antioxidant activities also contained high content of total phenolic and flavonoid. Twenty-eight compounds were identified as phenolic compounds and flavonoids by LC-MS/MS. The results illustrate that the E. angustifolium L., which is rich in phenolics, could be used as a natural resource of antioxidant ingredient.
Assuntos
Antioxidantes/farmacologia , Epilobium/química , Fenóis/análise , Extratos Vegetais/farmacologia , Antioxidantes/química , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Flavonoides/química , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Fenóis/química , Componentes Aéreos da Planta/química , Extratos Vegetais/análise , Extratos Vegetais/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismoRESUMO
One new triterpenoid (1) and 13 known compounds (2-14) were isolated from Schisandra pubescens stems. The structure of the new compound was established on the basis of 1D/2D NMR and HRESIMS spectroscopic analyses. The isolated compounds were evaluated for their hepatoprotective activities against D-GalN-induced cell injury in QSG7701 cells. Compounds 1, 13 and 14 at 10 µM showed hepatoprotective activities, with survival rates of 60.5, 50.4 and 48.9%, respectively.
Assuntos
Lignanas/farmacologia , Substâncias Protetoras/química , Substâncias Protetoras/farmacologia , Schisandra/química , Linhagem Celular , Avaliação Pré-Clínica de Medicamentos/métodos , Hepatócitos/efeitos dos fármacos , Humanos , Lignanas/química , Lignanas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Caules de Planta/química , Triterpenos/química , Triterpenos/farmacologiaRESUMO
One new compound, himalain A (1), together with 12 known compounds were isolated from Mirabilis himalaica. Their structures were determined by spectroscopic methods, including 2D NMR techniques, and the absolute configuration of the 1,2-diol moiety in 1 was defined through Riguera's method. All isolated compounds were tested for their cytotoxic and antibacterial activities.