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1.
Zhonghua Gan Zang Bing Za Zhi ; 28(4): 357-360, 2020 Apr 20.
Artigo em Zh | MEDLINE | ID: mdl-32403890

RESUMO

Liver sinusoidal endothelial cells (LSECs) are not only important intermediary cells for substance exchange between blood and hepatocytes, but also important hepatic non-parenchymal cells to cause liver fibrosis and cirrhosis because of chronic liver injury factors. It mainly regulates the liver microcirculation and participates in the development of hepatic fibrosis by interacting with hepatic stellate cells (HSCs), hepatocytes, Kupffer cells and mediating hepatic stiffness and hepatic angiogenesis. Hence, clarifying these mechanisms will help to explore new targets and strategies for the treatment of liver fibrosis.


Assuntos
Células Endoteliais/citologia , Cirrose Hepática/patologia , Fígado/citologia , Células Estreladas do Fígado , Hepatócitos , Humanos , Células de Kupffer , Fígado/patologia
2.
Genet Mol Res ; 13(3): 6142-7, 2014 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-25117373

RESUMO

Pancreatic cancer is a malignant neoplasm originating from transformed cells arising in tissues that form the pancreas. To investigate whether the tribbles homolog 1 (Drosophila) gene (TRIB1) is associated with pancreatic cancer in the Chinese Han population, we conducted this case-control study and genotyped 3 single nucleotide polymorphisms (rs2980879, rs2980874, and rs2235108) of the TRIB1 gene in 182 patients and 359 normal controls of Chinese Han origin and analyzed their association. The results showed that the rs2980879 polymorphism was associated with pancreatic cancer [allele: P = 0.023434, genotype: P = 0.03005; odds ratio (OR) and 95% confidence interval (CI) = 0.727788 (0.552664-0.958404)], whereas the rs2980874 polymorphism had no association with pancreatic cancer [allele: P = 0.749885, genotype: P = 0.699533; OR and 95%CI = 1.041981 (0.809196-1.341734)], and the rs2235108 polymorphism was not associated with the disease [allele: P = 0.629475, genotype: P = 0.547534, OR and 95%CI = 1.128290 (0.690829-1.842770)]. Haplotype analyses and linkage disequilibrium tests were also conducted, and the results showed that these 3 loci are not in the same block. In conclusion, our study indicated that the TRIB1 gene is associated with pancreatic cancer. More studies with larger samples are needed in order to support this finding.


Assuntos
Estudos de Associação Genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Neoplasias Pancreáticas/genética , Polimorfismo de Nucleotídeo Único , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , Idoso , Alelos , Estudos de Casos e Controles , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Haplótipos , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Razão de Chances , Proteínas Serina-Treonina Quinases/genética
3.
Artigo em Zh | MEDLINE | ID: mdl-37805734

RESUMO

Objective: To explore the effects of pedicled flap combined with membrane induction technique in repairing foot and ankle wounds in diabetic patients. Methods: A retrospective observational study was conducted. From March 2019 to July 2021, 12 patients with diabetic foot and ankle wounds who met the inclusion criteria were admitted to Wuxi Ninth People's Hospital, including 7 males and 5 females, aged 20 to 92 years. The wound area before debridement was 4.0 cm×2.5 cm to 16.0 cm×12.5 cm. The patients underwent debridement+antibiotic cement tamponade in stage Ⅰ; according to the wound site, peroneal artery perforator flap or posterior tibial artery perforator flap was chosen to repair the wound in stage Ⅱ, with the area of the resected flap ranging from 4.5 cm×3.0 cm to 18.5 cm×14.0 cm. The donor site was directly closed in 4 patients or covered by full-thickness inguinal skin graft in 8 patients. After the operation of stage Ⅱ, the survival of flap and skin graft, the scar in donor and recipient sites of flap, the appearance of flap, and the function of ankle joint of affected extremity were followed up. The recovery of foot and ankle function was evaluated and rated by the American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scoring System at the last follow-up. Results: During the follow-up of 4 to 15 months after the operation of stage Ⅱ, both the flap and skin graft survived, without obvious infection recurrence. Linear scars were left in donor and recipient sites of flap, with good appearance in flap. The function of ankle joint in the affected extremity was nearly normal. At the last follow-up, the AOFAS scores of patients were 79 to 93, with excellent in 8 cases and good in 4 cases. Conclusions: The pedicled flap combined with membrane induction technique for repairing foot and ankle wounds in diabetic patients has the advantage of simple operation, preserved ankle joint function, and less postoperative infection recurrence, which is worth popularizing in clinical practice.


Assuntos
Diabetes Mellitus , Pé Diabético , Retalho Perfurante , Procedimentos de Cirurgia Plástica , Lesões dos Tecidos Moles , Masculino , Feminino , Humanos , Tornozelo/cirurgia , Articulação do Tornozelo/cirurgia , Lesões dos Tecidos Moles/cirurgia , Transplante de Pele , Extremidade Inferior , Retalho Perfurante/irrigação sanguínea , Cicatriz/cirurgia , Pé Diabético/cirurgia , Resultado do Tratamento
4.
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi ; 39(12): 1158-1162, 2023 Dec 20.
Artigo em Zh | MEDLINE | ID: mdl-38129302

RESUMO

Objective: To explore the clinical efficacy of antibiotic bone cement combined with vacuum sealing drainage (VSD) in treating diabetes mellitus complicated with necrotizing fasciitis. Methods: The retrospective observational study approach was used. From January 2020 to March 2022, 12 patients with type 2 diabetes complicated with necrotizing fasciitis who met the inclusion criteria were admitted to Wuxi Ninth People's Hospital, including 7 males and 5 females, aged 27 to 76 years. The initial diagnosis of lesions was in the lower limbs. After admission, bedside incision and drainage were performed timely, and a sample of wound exudate was collected for microbial cultivation. At the same time, the comprehensive supportive treatment was performed. At stage Ⅰ, debridement was performed, and the skin and soft tissue defect area was 40 cm×15 cm to 80 cm×25 cm after debridement. The dead space was filled with bone cement containing gentamicin and vancomycin and VSD was performed. After there was no obvious infection on the wound, the antibiotic bone cement was removed and wound repair surgery was performed at stage Ⅱ. The times of debridement, amputation, infection control, wound treatment method and wound healing at stage Ⅱ, total hospitalization day, and recurrence of necrotizing fasciitis during follow-up after the stage Ⅱ surgery. At the last follow-up, the walking function of patients was evaluated according to the scoring standards of American Orthopedic Foot and Ankle Association (AOFAS). Results: Eleven patients had wound infection control with one debridement surgery and did not undergo amputation surgery; one patient had significant foot gangrene, and the infection was controlled after one debridement and amputation of the gangrenous limb. Blood routine and infection indicators gradually returned to normal within 7 days after surgery. At stage Ⅱ, the wounds in 4 patients were sutured directly, the wounds in 6 patients were repaired with full-thickness inguinal skin graft, while the wounds in 2 patients were repaired with pedicled or tongue-shaped flaps at the wound edge. The wounds healed well after surgery, with no ulceration. The total hospitalization day of patients was 20 to 45 days. Follow-up for 3 to 24 months after stage Ⅱ surgery showed no recurrence of necrotizing fasciitis in any patient. At the last follow-up, the walking function was evaluated as excellent in 10 cases and good in 2 cases according to the AOFAS scoring standard. Conclusions: Antibiotic bone cement combined with VSD used in treating type 2 diabetes complicated with necrotizing fasciitis can effectively control infection and reduce the times of debridement, with good wound healing and walking function after surgery.


Assuntos
Diabetes Mellitus Tipo 2 , Fasciite Necrosante , Tratamento de Ferimentos com Pressão Negativa , Lesões dos Tecidos Moles , Feminino , Humanos , Masculino , Antibacterianos/uso terapêutico , Cimentos Ósseos/uso terapêutico , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/cirurgia , Drenagem , Fasciite Necrosante/cirurgia , Extremidade Inferior , Tratamento de Ferimentos com Pressão Negativa/métodos , Transplante de Pele , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento , Adulto , Pessoa de Meia-Idade , Idoso
5.
Bone Joint Res ; 5(10): 461-469, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27754833

RESUMO

OBJECTIVES: The cytotoxicity induced by cobalt ions (Co2+) and cobalt nanoparticles (Co-NPs) which released following the insertion of a total hip prosthesis, has been reported. However, little is known about the underlying mechanisms. In this study, we investigate the toxic effect of Co2+ and Co-NPs on liver cells, and explain further the potential mechanisms. METHODS: Co-NPs were characterised for size, shape, elemental analysis, and hydrodynamic diameter, and were assessed by Transmission Electron Microscope, Scanning Electron Microscope, Energy Dispersive X-ray Spectroscopy and Dynamic Light Scattering. BRL-3A cells were used in this study. Cytotoxicity was evaluated by MTT and lactate dehydrogenase release assay. In order to clarify the potential mechanisms, reactive oxygen species, Bax/Bcl-2 mRNA expression, IL-8 mRNA expression and DNA damage were assessed on BRL-3A cells after Co2+ or Co-NPs treatment. RESULTS: Results showed cytotoxic effects of Co2+ and Co-NPs were dependent upon time and dosage, and the cytotoxicity of Co-NPs was greater than that of Co2+. In addition, Co-NPs elicited a significant (p < 0.05) reduction in cell viability with a concomitant increase in lactic dehydrogenase release, reactive oxygen species generation, IL-8 mRNA expression, Bax/Bcl-2 mRNA expression and DNA damage after 24 hours of exposure. CONCLUSION: Co-NPs induced greater cytotoxicity and genotoxicity in BRL-3A cells than Co2+. Cell membrane damage, oxidative stress, immune inflammation and DNA damage may play an important role in the effects of Co-NPs on liver cells.Cite this article: Y. K. Liu, X. X. Deng, H.L. Yang. Cytotoxicity and genotoxicity in liver cells induced by cobalt nanoparticles and ions. Bone Joint Res 2016;5:461-469. DOI: 10.1302/2046-3758.510.BJR-2016-0016.R1.

6.
Plant Cell Rep ; 18(3-4): 297-300, 1998 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30744239

RESUMO

Protoplasts isolated from `Page' tangelo (Minneola tangelo × clementine) cell suspension cultures were electrically fused with mesophyll protoplasts of orange jessamine [Murraya paniculata (L.) Jack]. Shoots were regenerated after 6 - 10 months of culture, but they were extremely recalcitrant to producing roots in root-induction medium. Complete plantlets were formed via micrografting. Chromosome counting of shoot tips revealed they were tetraploids (2n = 4x = 36). Glutamateoxaloacetate transaminase isozyme and randomly amplified polymorphic DNA analysis confirmed their hybridity. Orange jessamine is immune to citrus huanglongbin, a severe disease of citrus, but sexual incompatibility and limited graft compatibility exist between Citrus and orange jessamine. The cell fusion technique may make it possible to transfer the huanglongbin resistance trait from orange jessamine to Citrus.

7.
Tree Physiol ; 23(17): 1209-15, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-14597430

RESUMO

Valencia sweet orange (Citrus sinensis (L.) Osbeck) calluses were used as explants to develop a new transformation system for citrus mediated by Agrobacterium tumefaciens. Factors affecting Agrobacterium-mediated transformation efficiency included mode of pre-cultivation, temperature of cocultivation and presence of acetosyringone (AS). The highest transformation efficiency was obtained with a 4-day pre-cultivation period in liquid medium. Transformation efficiency was higher when cocultivation was performed for 3 days at 19 degrees C than at 23 or 28 degrees C. Almost no resistant callus was obtained if the cocultivation medium lacked AS. The transformation procedure yielded transgenic Valencia plants containing the pTA29-barnase gene, as verified by PCR amplification and confirmed by Southern blotting. Because male sterility is a common factor leading to seedlessness in citrus cultivars with parthenocarpic characteristics, production of seedless citrus genotypes by Agrobacterium-mediated genetic transformation is a promising alternative to conventional breeding methods.


Assuntos
Agrobacterium tumefaciens/genética , Citrus sinensis/genética , Tumores de Planta/genética , Plantas Geneticamente Modificadas/genética , Acetofenonas/metabolismo , Southern Blotting , Genes Bacterianos/genética , Vetores Genéticos/genética , Reação em Cadeia da Polimerase
8.
Appl Biochem Biotechnol ; 24-25: 193-9, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2162148

RESUMO

D-Xylose is a major constituent of hemicellulose, which makes up 20-30% of renewable biomass in nature. D-Xylose can be fermented by most yeasts, including Saccharomyces cerevisiae, by a two-stage process. In this process, xylose is first converted to xylulose in vitro by the enzyme xylose (glucose) isomerase, and the latter sugar is then fermented by yeast to ethanol. With the availability of an inexpensive source of xylose isomerase produced by recombinant E. coli, this process of fermenting xylose to ethanol can become quite effective. In this paper, we report that yeast xylose and xylulose fermentation can be further improved by cloning and overexpression of the xylulokinase gene. For instance, the level of xylulokinase activity in S. cerevisiae can be increased 230fold by cloning its xylulokinase gene on a high copy-number plasmid, coupled with fusion of the gene with an effective promoter. The resulting genetically-engineered yeast can ferment xylose and xylulose more than twice as fast as the parent yeast.


Assuntos
Genes Fúngicos , Fosfotransferases (Aceptor do Grupo Álcool) , Fosfotransferases/metabolismo , Saccharomyces cerevisiae/enzimologia , Leveduras/enzimologia , Clonagem Molecular , Escherichia coli/genética , Fermentação , Engenharia Genética , Cinética , Fosfotransferases/genética , Plasmídeos , Saccharomyces cerevisiae/genética , Leveduras/genética
9.
Appl Biochem Biotechnol ; 28-29: 369-75, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1929374

RESUMO

A plasmid-mediated transformation system has been developed for the xylose-fermenting yeast Pichia stipitis. We found that plasmid vectors containing the Saccharomyces cerevisiae 2 mu replicon and the kanamycin resistance gene (KmR) could be introduced into the Pichia cells and maintained as extrachromosomal elements. Pichia transformants containing such vectors will be resistant to the antibiotic geneticin that can be inactivated by the protein product of KmR. Plasmids identical to those used for transformation can be recovered from the Pichia transformants. Protocols for transformation of P. stipitis by the CaCl2-polyethylene glycol-protoplast process or by direct electroporation of intact Pichia cells have both been developed.


Assuntos
Pichia/genética , Saccharomyces cerevisiae/genética , Transformação Bacteriana , Xilose/metabolismo , Escherichia coli/genética , Fermentação , Vetores Genéticos , Resistência a Canamicina/genética , Pichia/crescimento & desenvolvimento , Pichia/metabolismo , Plasmídeos , Replicon , Leveduras/crescimento & desenvolvimento
10.
Yi Chuan Xue Bao ; 26(3): 244-8, 1999.
Artigo em Zh | MEDLINE | ID: mdl-10589164

RESUMO

The leaf indexes and stoma characteristics of leaf-parent-type regenerants were the same as those of the leaf parent Rough lemon, but quite different from the somatic hybrids. Leaf-parent-type plants were diploid (2n = 2x = 18). Patterns of POX, PPO, GOT isozymes were identical to those of Rough lemon. The plants were analyzed by using 54 arbitrary primers with polymorphisms. Most of the primers (52) showed that leaf-parent-type plants were in concordance with Rough lemon. However, with primer OPW-12, the plants contained a unique band of Hamlin sweet orange. Amplification products with primer OPV-04 showed slight differences among the accessions. The research suggested that most genetic constitutions of the leaf-parent-type plants were from leaf parent Rough lemon and a little from embryogenic suspension parent Hamlin sweet orange.


Assuntos
Citrus/genética , Hibridização Genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Regeneração
11.
Sheng Li Xue Bao ; 44(3): 254-60, 1992 Jun.
Artigo em Zh | MEDLINE | ID: mdl-1455257

RESUMO

The role of endothelium-derived relaxing factor (EDRF) on the effect of oxygen-derived free radicals (generated by xanthine-xanthine oxidase system) on intrapulmonary arterial in chronic hypoxic rats was studied by a microbioassay method. Intrapulmonary artery rings with intact or denuded endothelium of hypoxic (5,000 m, 10 days) and normoxic rats were prepared for observation of oxygen-derived free radicals induced contraction. It was shown that oxygen-derived free radicals induced contractions of intrapulmonary arterial rings with intact endothelium were obviously augmented in hypoxic rats than in normoxic controls. The augmented responses could be further potentiated by the addition of EDRF inactivator reduced hemoglobin (RHb), but diminished or even abolished by applying superoxide dismutase (Cu-Zn SOD). However, no effect on denuded rings was observed when RHb or SOD was added. It is concluded that chronic hypoxia may attenuate the action of EDRF in the enhancement of the reactivity of intrapulmonary artery to oxygen-derived free radicals.


Assuntos
Hipóxia/fisiopatologia , Óxido Nítrico/fisiologia , Oxigênio/metabolismo , Artéria Pulmonar/fisiopatologia , Vasoconstrição , Animais , Radicais Livres , Hipertensão Pulmonar/fisiopatologia , Técnicas In Vitro , Masculino , Ratos , Ratos Wistar
12.
Sheng Li Xue Bao ; 45(4): 382-6, 1993 Aug.
Artigo em Zh | MEDLINE | ID: mdl-8296214

RESUMO

The effect of hypoxia on intrapulmonary conversion of angiotensin I (A I) to angiotensin II (A II) was examined by using an isolated rat lung preparation perfused at constant flow and cultured calf pulmonary artery endothelial cells. The conversion rate of A I in endothelial cells of pulmonary vascular bed was found to be decreased in both acute hypoxic and chronic hypoxic (CH-5000, CH-8000) rats. The conversion rate of A I to A II was significantly higher in acute hypoxic rat lungs than that in denuded endothelial cells rat lungs. There was no significant difference between chronic hypoxic and denuded endothelial cells rat lungs. In cultured calf pulmonary artery endothelial cells (PAEC) exposed to hypoxia (3% O2) for 30 minutes the conversion of A I to A II was significantly reduced. In the time course of hypoxic exposure the conversion of A I to A II in cultured calf PAEC showed significant difference between hypoxia and normoxia. The results indicated that (1) Acute and chronic hypoxia impaired pulmonary conversion of A I to A II. (2) Effect of hypoxia on the pulmonary conversion of A I to A II is associated with the degree and the time of hypoxia.


Assuntos
Angiotensina II/biossíntese , Angiotensina I/metabolismo , Endotélio Vascular/metabolismo , Artéria Pulmonar/metabolismo , Animais , Hipóxia Celular , Células Cultivadas , Endotélio Vascular/citologia , Artéria Pulmonar/citologia , Ratos , Ratos Wistar
13.
Yao Xue Xue Bao ; 31(2): 132-7, 1996.
Artigo em Zh | MEDLINE | ID: mdl-8762472

RESUMO

The effect of ligustrazine hydrochloride (LTH) on depressing pulmonary artery hypertension has been proved in recent studies. In an attempt to prepare a lung targeting dosage form, ligustrazine hydrochloride gelatin microspheres (LTH-GMS) were prepared by the method of emulsion process, using 2:1 as the weight ratio of LTH to gelatin. The preparation technique was optimized and the appearance, particle size and size distribution, LTH content, in vitro release, stability, and in vivo distribution of LTH-GMS were studied. The results showed that the mean diameter of LTH-GMS measured by Coulter counter was 12.65 microns, 87.5% of the microspheres ranging from 5 to 24.9 microns. The average content of LTH in LTH-GMS was 16.49% +/- 0.49% (n = 3) with an average extent of entrapment of about 89% and the LTH-GMS were stable for three months when stored in refrigerator or at room temperature. The release profile in vitro (pH 7.8-8.0 phosphate buffer) can be described by first-order kinetic equation. The release t1/2 of LTH when given LTH-GMS was about 6 times as much as that of original LTH. The relative distribution percentage of LTH-GMS in lung when determined 20 min after iv administration to mice was significantly higher than those of LTH-GMS in other tissues and blood, and was about 6 times as much as that of the LTH solution control group in lung under the same conditions.


Assuntos
Pirazinas/administração & dosagem , Vasodilatadores/administração & dosagem , Animais , Portadores de Fármacos , Gelatina , Pulmão/metabolismo , Camundongos , Microesferas , Pirazinas/farmacocinética , Vasodilatadores/farmacocinética
14.
Front Biosci (Elite Ed) ; 4(2): 620-30, 2012 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-22201899

RESUMO

Peripheral blood genes expressions profiling (GeXP) have been convinced to be more specific for the diagnosis of cancer and other diseases, and the GeXP system provides an ideal method to analyze multiple genes expression in one normalized and equable system. We aim to differentiate hepatocellular carcinoma from other hepatic diseases based on peripheral blood and the GeXP system. Fifteen selected hepatic diseases related genes with two house-keeping genes for normalization were detected by the GeXP system. The diagnosis model was based on K nearest neighbor classifier and cross validation, and software based on MATLAB software was built for differential diagnosis of hepatic diseases. Eight hepatic related genes were demonstrated to show an obvious statistic difference in expressions while the K nearest neighbors classifier showed that the accuracy for normal controls, hepatitis B, liver cirrhosis, hepatocellular carcinoma and the Other group was separately 80.57 %, 78.17 %, 84.48 %, 73.24 % and 85.85 %. The set of validation has been carried out to assess the accuracy of Model Two and the accuracy was even higher than the set of building for the model, except for the hepatitis B (HBV) group. A sensitive and specific GeXP system of eight genes has been developed for the accurate differential diagnosis of hepatic disease.


Assuntos
Carcinoma Hepatocelular/diagnóstico , Perfilação da Expressão Gênica , Hepatopatias/diagnóstico , Neoplasias Hepáticas/diagnóstico , RNA Mensageiro/sangue , Adulto , Sequência de Bases , Carcinoma Hepatocelular/sangue , Carcinoma Hepatocelular/genética , Estudos de Casos e Controles , Primers do DNA , Diagnóstico Diferencial , Humanos , Hepatopatias/sangue , Hepatopatias/genética , Neoplasias Hepáticas/sangue , Neoplasias Hepáticas/genética , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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