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We present a GPU accelerated toolbox for shaping the light propagation through multimode fibre using a spatial light modulator (SLM). The light is modulated before being coupled to the proximal end of the fibre in order to achieve arbitrary light patterns at the distal end of the fibre. First, the toolbox optimises the acquisition time of the transformation matrix of the fibre by synchronous operation of CCD and SLM. Second, it uses the acquired transformation matrix retained within the GPU memory to design, in real-time, the desired holographic mask for on-the-fly modulation of the output light field. We demonstrate the functionality of the toolbox by acquiring the transformation matrix at the maximum refresh rate of the SLM - 204 Hz, and using it to display an on-demand oriented cube, at the distal end of the fibre. The user-controlled orientation of the cube and the corresponding holographic mask are obtained in 20 ms intervals. Deleterious interference effects between the neighbouring points are eliminated by incorporating an acousto-optic deflector (AOD) into the system. We remark that the usage of the toolbox is not limited to multimode fibres and can be readily used to acquire transformation matrix and implement beam-shaping in any other linear optical system.
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Evolution shows that photonic structures are a constituent part of many animals and flora. These elements produce structural color and are useful in predator-prey interactions between animals and in the exploitation of light for photosynthetic organisms. In particular, diatoms have evolved patterned hydrated silica external valves able to confine light with extraordinary efficiency. Their evolution was probably guided by the necessity to survive in harsh conditions of sunlight deprivation. Here, we exploit such diatom valves, in conjunction with structured illumination, to realize a biological super-resolving lens to achieve sub-diffractive focusing in the far field. More precisely, we consider a single diatom valve of Arachnoidiscus genus which shows symmetries and fine features. By characterizing and using the transmission properties of this valve using the optical eigenmode technique, we are able to confine light to a tiny spot with unprecedented precision in terms of resolution limit ratio, corresponding in this case to 0.21λ/NA.
Assuntos
Diatomáceas/fisiologia , Fenômenos Ópticos , Animais , Simulação por Computador , Diatomáceas/ultraestrutura , Lentes , Análise Numérica Assistida por ComputadorRESUMO
We report on the improvement of the infrared optical trapping efficiency of dielectric microspheres by the controlled adhesion of gold nanorods to their surface. When trapping wavelength was equal to the surface plasmon resonance wavelength of the gold nanorods (808 nm), a 7 times improvement in the optical force acting on the microspheres was obtained. Such a gold nanorod assisted enhancement of the optical trapping efficiency enabled the intracellular manipulation of the decorated dielectric microsphere by using a low power (22 mW) infrared optical trap.
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Ouro/química , Espaço Intracelular/metabolismo , Microesferas , Nanotubos/química , Fenômenos Ópticos , Dióxido de Silício/química , Animais , Linhagem Celular , Sobrevivência Celular , Macrófagos/metabolismo , Camundongos , Nanotubos/ultraestrutura , Imagem Óptica , Pinças ÓpticasRESUMO
We present a generic technique allowing size-based all-optical sorting of gold nanoparticles. Optical forces acting on metallic nanoparticles are substantially enhanced when they are illuminated at a wavelength near the plasmon resonance, as determined by the particle's geometry. Exploiting these resonances, we realize sorting in a system of two counter-propagating evanescent waves, each at different wavelengths that selectively guide nanoparticles of different sizes in opposite directions. We validate this concept by demonstrating bidirectional sorting of gold nanoparticles of either 150 or 130 nm in diameter from those of 100 nm in diameter within a mixture.
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PURPOSE: To quantify how often physical therapists from high-income countries (HIC) travelling to low- and middle-income countries (LMIC) practise outside their scope of practice, in what circumstances, and their likelihood of doing the same in the future. METHODS: An exploratory descriptive study using a survey. RESULTS: One hundred and twenty-six licensed physical therapists from around the world participated. Physical therapists typically spent less than a month (73.8 per cent) in LMIC; 67.5 per cent believed that physical therapists practise outside of their scope, and 31.7 per cent reported doing so. Reasons were believing that something is better than nothing (47.5 per cent ), a mismatch between the physical therapist's and host's expectations (40.0 per cent ), and preserving their relationship with the host (25.0 per cent ). It was deemed appropriate by 64.5 per cent to practise outside of their scope in some situations and 53.8% considered repeating the activity in the future. Half of the respondent's first experience in LMIC occurred as a student or in their first decade of practice. CONCLUSIONS: Working in LMIC requires a keen understanding of the risks and challenges associated with such experiences. To ensure best practice, a skill set that consists of critical self-reflection, systems thinking, and structural competency combined with clinical competency and accountability is imperative.
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Países em Desenvolvimento , Fisioterapeutas , Humanos , Países Desenvolvidos , Inquéritos e Questionários , Conhecimentos, Atitudes e Prática em SaúdeRESUMO
We report a mathematically rigorous technique which facilitates the optimization of various optical properties of electromagnetic fields in free space and including scattering interactions. The technique exploits the linearity of electromagnetic fields along with the quadratic nature of the intensity to define specific Optical Eigenmodes (OEi) that are pertinent to the interaction considered. Key applications include the optimization of the size of a focused spot, the transmission through sub-wavelength apertures, and of the optical force acting on microparticles. We verify experimentally the OEi approach by minimising the size of a focused optical field using a superposition of Bessel beams.
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Transferência de Energia , Modelos Teóricos , Pinças Ópticas , Refratometria/métodos , Espalhamento de Radiação , Simulação por Computador , Campos EletromagnéticosRESUMO
We explore the passive optical sorting of plasmon nanoparticles and investigate the optimal wavelength and optimal beam shape of incident field. The condition for optimal wavelength is found by maximising the nanoparticle separation whilst minimising the temperature increase in the system. We then use the force optical eigenmode (FOEi) method to find the beam shape of incident electromagnetic field, maximising the force difference between plasmon nanoparticles. The maximum force difference is found with respect to the whole sorting region. The combination of wavelength and beam shape study is demonstrated for a specific case of gold nanoparticles of radius 40 nm and 50 nm respectively. The optimum wavelength for this particular situation is found to be above 700 nm. The optimum beam shape depends upon the size of sorting region and ranges from plane-wave illumination for infinite sorting region to a field maximising gradient force difference in a single point.
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Standardization and quality monitoring of alcoholic beverages is an important issue in the liquor production industry. Various spectroscopic techniques have proved useful for tackling this problem. An ideal sensing device for alcoholic beverages should be able to detect the quality of alcohol with a small amount of sample at a low acquisition time using a portable and easy to use device. We propose the use of near infra-red spectroscopy on an optofluidic chip for quality monitoring of single malt Scotch whisky. This is chip upon which we have previously realized waveguide confined Raman spectroscopy. Analysis on this alignment-free, portable chip may be performed in only 2 seconds with a sample volume of only 20 µl. Using a partial least square (PLS) calibration, we demonstrate that the alcohol content in the beverage may be predicted to within a 1% prediction error. Principal component analysis (PCA) was employed for successful classification of whiskies based upon their age, type and cask. The prospect of implementing an optofluidic analogue of a conventional fiber based spectroscopic probe allows a rapid analysis of alcoholic beverages with dramatically reduced sample volumes.
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Bebidas Alcoólicas/análise , Técnicas Analíticas Microfluídicas/métodos , Óptica e Fotônica/métodos , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Análise por Conglomerados , Etanol/análise , Análise dos Mínimos Quadrados , Fotodegradação , Análise de Componente Principal , Reprodutibilidade dos Testes , Análise Espectral RamanRESUMO
The study and application of optical vortices have gained significant prominence over the last two decades. An interesting challenge remains the determination of the azimuthal index (topological charge) â of an optical vortex beam for a range of applications. We explore the diffraction of such beams from a triangular aperture and observe that the form of the resultant diffraction pattern is dependent upon both the magnitude and sign of the azimuthal index and this is valid for both monochromatic and broadband light fields. For the first time we demonstrate that this behavior is related not only to the azimuthal index but crucially the Gouy phase component of the incident beam. In particular, we explore the far field diffraction pattern for incident fields incident upon a triangular aperture possessing non-integer values of the azimuthal index â. Such fields have a complex vortex structure. We are able to infer the birth of a vortex which occurs at half-integer values of â and explore its evolution by observations of the diffraction pattern. These results demonstrate the extended versatility of a triangular aperture for the study of optical vortices.
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Iluminação/métodos , Modelos Teóricos , Refratometria/métodos , Simulação por Computador , Luz , Espalhamento de RadiaçãoRESUMO
We report a novel fiber probe based Raman detection system on a microfluidic platform where a split Raman probe is directly embedded into a polydimethylsiloxane (PDMS) chip. In contrast to previous Raman detection schemes in microfluidics, probe based detection offers reduced background and portability. Compared to conventional backscattering probe designs, the split fiber probe we used in this system, results in a reduced size and offers flexibility to modify the collection geometry to minimize the background generated by the fibers. Also our microfluidic chip design enables us to obtain an alignment free system. As a proof of concept we demonstrate the sensitivity of the device for urea detection at relevant human physiological levels with a low acquisition time. The development of this system on a microfluidic platform means portable, lab on a chip devices for biological analyte detection and environmental sensing using Raman spectroscopy are now within reach.
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Microfluídica/instrumentação , Microfluídica/métodos , Fibras Ópticas , Análise Espectral Raman/instrumentação , Análise Espectral Raman/métodos , Etanol/análise , Limite de Detecção , Reologia , Fatores de Tempo , Ureia/análiseRESUMO
We present an experimental and theoretical study of long distance optical binding effects acting upon micro-particles placed in a standing wave optical field. In particular we present for the first time quantitatively the binding forces between individual particles for varying inter-particle separations, polarizations and incident angles of the binding beam. Our quantitative experimental data and numerical simulations show that these effects are essentially enhanced due to the presence of a reflective surface in a sample chamber. They also reveal conditions to form stable optically bound clusters of two and three particles in this geometry. We also show that the inter-particle separation in the formed clusters can be controlled by altering the angle of the beam incident upon the sample plane. This demonstrates new perspectives for the generation and control of optically bound soft matter and may be useful to understand various inter-particle effects in the presence of reflective surfaces.
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We describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging.
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Cromatografia/instrumentação , Tecnologia de Fibra Óptica/instrumentação , Análise de Injeção de Fluxo/instrumentação , Técnicas Analíticas Microfluídicas/instrumentação , Espectrometria de Fluorescência/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , FótonsRESUMO
We utilize advanced laser fields to clear a path through a dynamic turbid medium, a concept termed "Optical path clearing (OPC)." Particles are evacuated from a volume of the medium using the gradient and/or scattering forces due to an applied laser field with a suitably tailored spatial profile. Our studies encompass both an analytical model and proof-of-principle experiments where paths are cleared in dense bulk colloidal suspensions. Based on our results we suggest that high-performance and high efficiency OPC will be achieved by multiple-step clearing using dynamic laser fields based on Airy or inverted axicon beams.
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Eletrônica/instrumentação , Lasers , Luz , Processamento de Sinais Assistido por Computador/instrumentação , Suspensões/química , Desenho de EquipamentoRESUMO
The response of a microscopic dielectric object to an applied light field can profoundly affect its kinetic motion. A classic example of this is an optical trap, which can hold a particle in a tightly focused light beam. Optical fields can also be used to arrange, guide or deflect particles in appropriate light-field geometries. Here we demonstrate an optical sorter for microscopic particles that exploits the interaction of particles-biological or otherwise-with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice. The strength of this interaction with the lattice sites depends on the optical polarizability of the particles, giving tunable selection criteria. We demonstrate both sorting by size (of protein microcapsule drug delivery agents) and sorting by refractive index (of other colloidal particle streams). The sorting efficiency of this method approaches 100%, with values of 96% or more observed even for concentrated solutions with throughputs exceeding those reported for fluorescence-activated cell sorting. This powerful, non-invasive technique is suited to sorting and fractionation within integrated ('lab-on-a-chip') microfluidic systems, and can be applied in colloidal, molecular and biological research.
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Fracionamento por Campo e Fluxo/métodos , Luz , Microfluídica , Movimento (Física) , Cápsulas/química , Coloides/química , Fracionamento por Campo e Fluxo/instrumentação , Óptica e Fotônica , Tamanho da PartículaRESUMO
We experimentally probed the nonlinear optical response of aqueous nano-colloidal suspensions to provide a test of the theoretical approaches that have been proposed for the nonlinearity, namely an exponential model, an artificial Kerr medium, and a non-ideal gas model. The best agreement with experiment is found using the non-ideal gas model for the colloidal suspension which in turn can be used to infer values for the second virial coefficient of the medium and the nonlinear coefficients.
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Coloides/química , Modelos Químicos , Nefelometria e Turbidimetria/métodos , Refratometria/métodos , Simulação por Computador , Dinâmica não Linear , Espalhamento de RadiaçãoRESUMO
We generate a broadband "white light" Airy beam and characterize the dependence of the beam properties on wavelength. Experimental results are presented showing that the beam's deflection coefficient and its characteristic length are wavelength dependent. In contrast the aperture coefficient is not wavelength dependent. However, this coefficient depends on the spatial coherence of the beam. We model this behaviour theoretically by extending the Gaussian-Schell model to describe the effect of spatial coherence on the propagation of Airy beams. The experimental results are compared to the model and good agreement is observed.
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Common-path optical coherence tomography (CPOCT) is known to reduce group velocity dispersion and polarization mismatch between the reference and the sample arm as both arms share the same physical path. Existing implementations of CPOCT typically require one to incorporate an additional cover glass within the beam path of the sample arm to provide a reference signal. In this paper, we aim to further reduce this step by directly making use of the back-reflected signal, arising from a conical lens-tip fiber, as a reference signal. The conical lens, which is directly manufactured onto the optical fiber tip via a simple selective-chemical etching process, fulfils two functions acting as both the imaging lens and the self-aligning reference plane. We use a Fourier-domain OCT system to demonstrate the feasibility of this technique upon biological tissue. An in-fiber CPOCT technique may prove potentially useful in endoscopic OCT imaging.
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Endoscopia , Tecnologia de Fibra Óptica/instrumentação , Aumento da Imagem/instrumentação , Tomografia de Coerência Óptica/instrumentação , Transdutores , Desenho Assistido por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
We demonstrate controlled rotation of optically trapped objects in a spiral interference pattern. This pattern is generated by interfering an annular shaped laser beam with a reference beam. Objects are trapped in the spiral arms of the pattern. Changing the optical path length causes this pattern, and thus the trapped objects, to rotate. Structures of silica microspheres, microscopic glass rods, and chromosomes are set into rotation at rates in excess of 5 hertz. This technique does not depend on intrinsic properties of the trapped particle and thus offers important applications in optical and biological micromachines.
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Cromossomos , Lasers , Micromanipulação , Rotação , Animais , Cricetinae , Cricetulus , Vidro , Microesferas , Óptica e Fotônica , Dióxido de SilícioRESUMO
We report on the optical trapping of water droplets with a supercontinuum laser source. Droplet size is determined by observing the spectrum of the on-axis backscattered light. In contrast to to monochromatic trapping, the broad spectrum of the supercontinuum covers several resonances of the first excited Mie coefficients. A minimum value of Q approximately 0.16 for the trapping efficiency is estimated.
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Aerossóis/isolamento & purificação , Desenho Assistido por Computador , Monitoramento Ambiental/instrumentação , Modelos Teóricos , Pinças Ópticas , Análise Espectral/instrumentação , Água/análise , Simulação por Computador , Desenho de Equipamento , Análise de Falha de EquipamentoRESUMO
Optically guided neuron growth is a relatively new field where the exact mechanisms that initiate growth are not well understood. Both Gaussian light beams and optical line traps have been purported to initiate neuronal growth. Here we present a detailed study using optical line traps with symmetric and asymmetric intensity profiles which have been previously reported to bias the direction of neuronal growth. In contrast to these previous studies, we show similar levels of growth regardless of the direction of the intensity variation along the line trap. Furthermore, our experimental observations confirm previous suggestions that the filopodia produced from neuronal growth cones can be affected by laser light. We experimentally observe alignment of filopodia with the laser field and present a theoretical model describing the optical torques experienced by filopodia to explain this effect.