Effect of reactor operation modes on mitigating antibiotic resistance genes (ARGs) and methane production from hydrothermally-pretreated pig manure.

Numerous efforts have been made to enhance the performance of anaerobic digestion (AD) for accelerating renewable energy generation, however, it remains unclear whether the intensified measures could enhance the proliferation and transmissions of antibiotic resistance genes (ARGs) in the system. This study assessed the impact of an innovative pig manure AD process, which includes hydrothermal pretreatment (HTP) and a two-stage configuration with separated acidogenic and methanogenic phases, on biomethane (CH4) production and ARGs dynamics. Results showed that HTP significantly increase CH4 production from 0.65 to 0.75 L/L/d in conventional single-stage AD to 0.82 and 0.91 L/L/d in two-stage AD. This improvement correlated with a rise in the relative abundance of Methanosarcina, a key methanogenesis microorganism. In the two-stage AD, the methanogenic stage offered an ideal environment for methanogens growth, resulting in substantially faster and higher CH4 production by about 10% compared to single-stage AD. Overall, the combined use of HTP and the two-stage AD configuration enhanced CH4 production by 40% compared to traditional single-stage AD. The abundance and diversity of ARGs were significantly reduced in the acidogenic reactors after HTP. However, the ARGs levels increased by about two times in the following methanogenesis stage and reached similar or higher levels than in single stage AD. The erm(F), erm(G), ant(6)-Ia, tet(W), mef(A) and erm(B) were the six main ARGs with significant differences in relative abundances in various treatments. The two-stage AD mode could better remove sul2, but it also had a rebound which elevated the risk of ARGs to the environment and human health. Network analysis identified pH and TVFAs as critical factors driving microbial communities and ARG proliferation in the new AD process. With the results, this study offers valuable insights into the trade-offs between AD performance enhancement and ARG-related risks, pinpointing essential areas for future research and practical improvements.

Exploring the influence of sulfadiazine-induced stress on antibiotic removal and transformation pathway using microalgae Chlorella sp.

Sulfadiazine (SDZ) is a kind of anti-degradable antibiotics that is commonly found in wastewater, but its removal mechanism and transformation pathway remain unclear in microalgal systems. This study investigated the effects of initial algae concentration and SDZ-induced stress on microalgal growth metabolism, SDZ removal efficiency, and transformation pathways during Chlorella sp. cultivation. Results showed that SDZ had an inhibitory effect on the growth of microalgae, and increasing the initial algal biomass could alleviate the inhibitory effect of SDZ. When the initial algal biomass of Chlorella sp. was increased to 0.25 g L-1, the SDZ removal rate could reach 53.27%-89.07%. The higher the initial algal biomass, the higher the SOD activity of microalgae, and the better the protective effect on microalgae, which was one of the reasons for the increase in SDZ removal efficiency. Meanwhile, SDZ stress causes changes in photosynthetic pigments, lipids, total sugars and protein content of Chlorella sp. in response to environmental changes. The main degradation mechanisms of SDZ by Chlorella sp. were biodegradation (37.82%) and photodegradation (23%). Most of the degradation products of SDZ were less toxic than the parent compound, and the green algae were highly susceptible to SDZ and its degradation products. The findings from this study offered valuable insights into the tradeoffs between accumulating microalgal biomass and antibiotic toxic risks during wastewater treatment, providing essential direction for the advancement in future research and full-scale application.

Carbon footprint of dairy manure management chains in response to nutrient recovery by aerobic pre-treatment.

Aerobic pre-treatment of liquid dairy manure has previously been reported as an effective nutrient export and emissions mitigation approach. The first objective of this study was to experimentally determine the optimal intermittent aeration ratio for nutrient recovery from liquid dairy manure through an on-site pilot-scale reactor to partially reduce the required energy for the aerobic process. The second objective was to theoretically investigate the total carbon footprints of direct manure spreading on croplands and permanent manure storage in open anaerobic lagoons in response to nutrient removal by the optimal determined intermittent aerobic treatment ratio. Four scenarios (S) were included; S1 was the traditional scenario of manure spread on croplands without the aerobic pre-treatment, S2 was the modified scenario of manure spread on croplands that included the aerobic pre-treatment, S3 was the traditional scenario of manure storage in lagoons, and S4 was the modified scenario of manure storage in lagoons that included the aerobic pre-treatment. The results showed that comparable nutrient removal efficiencies could be obtained with a 5:1 intermittent aeration ratio. Total nitrogen (TN) and total phosphorus (TP) were recovered were 41.5 ± 1.3% and 37.0 ± 4.0%, respectively, in ammonium sulfate and phosphorus-rich sludge, while 55.3 ± 1.4% of the chemical oxygen demand (COD) was removed. The estimated total carbon footprint for S1, S2, S3, and S4 were 24.4, 37.9, 45.3, and 45.9 kg CO2-eqton-1, respectively. However, the total carbon footprint of S2' and S4', which used renewable-based energy to run the reactor instead of fossil-based energy used in S2 and S4, were estimated to 29.5 and 37.5 kg CO2-eqton-1, respectively. Clearly, applying the aerobic pre-treatment increased the total carbon footprint of all cases except S4', in which the total carbon footprint was mitigated by -17.2%. Accordingly, the aerobic pre-treatment is only recommended in the case of S4' from a carbon footprint point of view although it is an effective nutrient recovery technology.

Revealing the link between evolution of electron transfer capacity of humic acid and key enzyme activities during anaerobic digestion.

Humic acid (HA) is an important active compound formed during anaerobic digestion process, with a complex structure and dynamic electron transfer capacity (ETC). However, the mechanisms by which these macromolecular organic compounds dynamically interact with the microbial anaerobic digestion process at different operating temperatures are still unclear. In this study, the link between the evolution of the ETC of HAs and the microbial community under mesophilic and thermophilic conditions was investigated. The results showed an increasing trend in the ETC of HAs in both mesophilic (671-1479 µmol gHA-1) and thermophilic (774-1506 µmol gHA-1) anaerobic digestion (AD) until day 25. The ETC was positively correlated with the bacterial community of hydrolytic and acidogenic phases, but negatively correlated with the archaeal community of the methanogenic phase. Furthermore, the relationship between ETC and key enzyme activity was explored using a co-occurrence network analysis. HAs revealed a high potential to promote key enzyme activities during hydrolysis (amylase and protease) and acidification (acetate kinase, butyrate kinase, and phosphotransacetylase) while inhibiting the key enzyme activity in the methanogenic phase during the anaerobic digestion process. Moreover, HAs formed under thermophilic conditions had a greater influence on key enzyme activities than those formed under mesophilic conditions. This study advances our understanding of the mechanisms underlying the influence of HAs on anaerobic digestion performance.

Predicting membrane fouling in a high solid AnMBR treating OFMSW leachate through a genetic algorithm and the optimization of a BP neural network model.

Anaerobic membrane bioreactors are a promising technology in the treatment of high-strength wastewater; however, unpredictable membrane fouling largely limits their scale-up application. This study, therefore, adopted a backpropagation neural network model to predict the membrane filtration performance in a submerged system, which treats leachate from the organic fraction of municipal solid waste. Duration time, water yield flow, influent COD, pH, bulk sludge concentration, and the ratio of ΔTMP to filtration time were selected as input variables to simulate membrane permeability. The membrane pressure slightly increased by 1.1 kPa within 62 days of operation. The results showed that the AnMBR membrane filtration performance was acceptable when treating OFMSW leachate under a flux of 6 L/(m2·h). The model results indicated that the sludge concentration largely determined the membrane fouling with a contribution of 33.8%. Given the local minimization problem in the BP neural network process, a genetic algorithm was introduced to optimize the simulation process, and the relative error of the results was reduced from 5.57% to 3.57%. Conclusively, the artificial neural network could be a useful tool for the prediction of an AnMBR that is so far under development.

The potential co-benefits for health, economy and climate by substituting raw coal with waste cooking oil as a winter heating fuel in rural households of northern China.

The toxic emissions from coal combustion associated with domestic winter heating requirements are an important public health issue. Waste cooking oil (WCO) holds promise as a means of reducing pollutant emissions thereby improving human health with the co-benefit of decreasing climate-forcing gas emissions by avoiding the combustion of mineral coal. With an annual production of ~2.17 Mt of WCO in Northern China, it could be used to meet the winter heating demand of ~3.25 million rural households, offsetting ~9.83 Mt of raw coal consumption. Through the adoption of coal-to-WCO shift in rural regions of 15 provinces, approximately 15.0%, 15.6%, 15.9% and 13.7%, respectively of CO, PM2.5, SO2 and NOX emissions would be eliminated. It is estimated that such a change would remove the respective contributions of these pollutants to the premature deaths of respectively, 63,400, 29,300, 173,00 and 31,300 rural residents. Such a positive health impact on the labor cohort would reduce the loss of labor supply and work time, as well as producing billions of RMB in economic benefits. WCO-based heating technology has the same effect on the reduction of GWC100 value as other modern energy carriers while also being cheaper and sustainable, long term. Reducing household emissions by substituting raw coal with green energy is a vital strategy to support pathways for sustainable environment design. The results of this work for the coal-to-WCO shift can reinforce the support for coal phase-out in China.

Nutrients recovery from fresh liquid manure through an airlift reactor to mitigate the greenhouse gas emissions of open anaerobic lagoons.

Open anaerobic lagoons are widely used for liquid manure storage and treatment, with excess greenhouse gas (GHG) and odor emissions. In this study, liquid manure was valorized through hybrid nitrogen and phosphorous recovery as value-added products using an airlift reactor. Also, the organic load of liquid manure was reduced before discharging into anaerobic lagoons, which simultaneously mitigated GHG emissions. The results showed that 14.5% of total nitrogen (TN) was recovered as ammonium sulfate, while 38.8% of TN and 79.3% of total phosphorus (TP) were recovered as phosphorus-rich sludge. After the pre-treatment in the reactor, the odor could be controlled effectively due to a 94.2% decrease in total VFAs. In addition, 59.0% of COD was removed, which decreased the theoretical modeled GHG emissions by 51.7% compared to the traditional direct discharging. The application is promising for upgrading anaerobic lagoons of liquid manure.

Upgrading Solid Digestate from Anaerobic Digestion of Agricultural Waste as Performance Enhancer for Starch-Based Mulching Biofilm.

Developing a green and sustainable method to upgrade biogas wastes into high value-added products is attracting more and more public attention. The application of solid residues as a performance enhancer in the manufacture of biofilms is a prospective way to replace conventional plastic based on fossil fuel. In this work, solid digestates from the anaerobic digestion of agricultural wastes, such as straw, cattle and chicken manures, were pretreated by an ultrasonic thermo-alkaline treatment to remove the nonfunctional compositions and then incorporated in plasticized starch paste to prepare mulching biofilms by the solution casting method. The results indicated that solid digestate particles dispersed homogenously in the starch matrix and gradually aggregated under the action of a hydrogen bond, leading to a transformation of the composites to a high crystalline structure. Consequently, the composite biofilm showed a higher tensile strength, elastic modulus, glass transition temperature and degradation temperature compared to the pure starch-based film. The light, water and GHG (greenhouse gas) barrier properties of the biofilm were also reinforced by the addition of solid digestates, performing well in sustaining the soil quality and minimizing N2O or CH4 emissions. As such, recycling solid digestates into a biodegradable plastic substitute not only creates a new business opportunity by producing high-performance biofilms but also reduces the environmental risk caused by biogas waste and plastics pollution.

Probing the efficiency of magnetically modified biomass-derived biochar for effective phosphate removal.

Characterization of the driving forces for effective and economical phosphate (PO43-) removal from wastewater by using magnetically modified biochar was performed in this study. The biochar produced from slow pyrolysis of local agricultural biomass (wood and rice husks) were magnetically modified by co-precipitation of Fe(II) and Fe(III) ions in their presence. The surface characteristics before and after modification and their efficacy for PO43- sorption, and desorption were compared. Results show that, even though magnetic biochar surface modification slightly decreased their surface area, PO43- adsorption to the modified biochars was almost double (25-28 mg g-1) than that to the raw biochar (12-15 mg g-1). The adsorption isotherm of raw biochars was better simulated via the Langmuir model, while that of modified biochars was better fitted to the Freundlich model. Moreover, the integrated analysis by XRD, EDX, and FTIR show that PO43- sorption to modified biochars could be attributed to the simultaneously-occurring electrostatic attraction, surface precipitation, and ligand exchange. While the electrostatic attraction was dominant in the presence of unmodified biochars. The regenerated modified biochars retained substantial PO43- adsorption capacity up to several regeneration cycles. Their high reusability potential leads to the effective and economical phosphate recovery and thus modified biochars could offer a viable strategy for PO43- removal.

Use of nano-/micro-magnetite for abatement of cadmium and lead contamination.

Structural variations of a mineral dictate its adsorption capacity which affects the mobility and toxicity of contaminants in natural and engineered systems. Present batch study evaluates the adsorption of lead (Pb) and cadmium (Cd) onto three magnetites having nanometric (M1-30 nm and M2-60 nm) and micrometric particle sizes (M3-1.5 µm). Obtained data revealed that particle size of tested magnetites strongly affected the extent and kinetics of metal adsorption and desorption. Observed order of adsorption efficiency was M1 > M2 > M3 with optimum monolayer adsorption of 408.14, 331.40, 178.47 mg/g (for Pb) and 228.05, 170.86, 83.49 mg/g (for Cd), respectively. Adsorption data were well fitted to the Freundlich (R2 = 0.99), Langmuir (R2 = 0.99) and pseudo-first order models (R2 = 0.98). Electrostatic attraction and surface precipitation interaction via external mass transfer between bulk liquid-solid interfaces were the potential adsorption pathways. Pb showed higher affinity than Cd in multi-metal system. Desorption efficiency was higher in acidic environment (92%) than in distilled water (44%). Moreover, regenerated magnetite samples retained good adsorption capacity for six cycles. As soils are characterized by large variability of iron minerals, these findings have important implications regarding the transport and immobilization of contaminants particularly in the management of contaminated soils.

Formation of struvite from agricultural wastewaters and its reuse on farmlands: Status and hindrances to closing the nutrient loop.

To meet the needs of a fast growing global population, agriculture and livestock production have been intensified, resulting in environmental pollution, climate change, and soil health declining. Closing the nutrient circular loop is one of the most important sustainability factors that affect these issues. Apart from being a serious environmental issue, the discharge of N and P via agricultural wastewater is also a major factor that disturbs nutrient cycling in agriculture. In this study, the performance, in terms of recovery, of N and P (individually, as well as simultaneously) from agricultural wastewaters via struvite has been comparatively summarized. Details on the hindrances to nutrient recovery through struvite formation from agricultural effluents, along with strategies to overcome these hindrances, are provided. In addition, various strategies for recovery performance intensification and operational cost reduction are comprehensively discussed. This work will provide scientists and engineers with a better idea on how to solve the bottlenecks of this technique and integrate it successfully into their treatment systems, which will ultimately help close the nutrient loop in agriculture.

Enhancing anaerobic digestion of dairy and swine wastewater by adding trace elements: evaluation in batch and continuous experiments.

Trace elements play a critical role for microbial activity in anaerobic digestion (AD) but their effects were probably overestimated in batch tests and should be comparably evaluated in continuous systems. In this study, Fe2+, Co2+, Ni2+, Cu2+ and Zn2+ were added in different concentrations to manure wastewater, and the effects were compared in both batch and continuous systems. The results were used to demonstrate suitable trace element compositions for AD of dairy and swine wastewater, and to compare the outcomes from batch and continuous systems. Fe2+ and Zn2+ were identified as being the most efficient stimulant of dairy and swine wastewater respectively. The addition of 5 mg/L Fe2+ and 0.4 mg/L Zn2+ increased the batch specific methane yield by 62% and 126% for dairy and swine wastewater, respectively. Nevertheless, a lower increment of 2% and 21%, for dairy and swine wastewater was obtained in the 120-day continuously-fed experiments. The 16S rRNA gene sequencing results indicated a relationship between the methanogens population, specific methanogenic activities, propionate, and dissolved hydrogen. Conclusively, the addition of a low dosage of Fe2+ and Zn2+ is a feasible strategy to enhance the methanogenic metabolism of the AD of dairy and swine wastewater respectively.

Size-dependent dual emission of Cu,Mn:ZnSe QDs: Controlling both emission wavelength and intensity.

Cu,Mn:ZnSe quantum dots (QDs) of tunable size, controllable photoluminescence (PL) intensity ratio and PL range were prepared. A study of the experimental conditions confirmed that the size of Cu,Mn:ZnSe QDs is affected by the pH of the solution, the speed at which the Zn solution is injected and the reaction temperature. In general, high pH, low injection speed and high reaction temperature are optimal for preparing large QDs. Based on this knowledge, different sizes of Cu,Mn:ZnSe QDs were synthesized. Moreover, white emission Cu,Mn:ZnSe QDs were designed by controlling the experimental conditions and the feeding mole ratio of Mn:Cu.

Phosphate recovery from liquid fraction of anaerobic digestate using four slow pyrolyzed biochars: Dynamics of adsorption, desorption and regeneration.

Four slow pyrolyzed biochars produced from wood (WDB), corncobs (CCB), rice husks (RHB) and sawdust (SDB) were evaluated for adsorption, desorption and regeneration of phosphate (PO43--P) from anaerobically digested liquid swine manure. The PO43--P adsorption capacity increased followed by initial concentrations increasing. Maximum PO43--P adsorptions at initial 150 mg/L of PO43--P (highest load) were average of 7.67, 6.43, 5.73 and 5.41 mg/g for WDB, CCB, RHB and SDB, respectively. Pseudo second order kinetics model could best fit PO43--P adsorption, which indicated the chemisorption via precipitation was the main mechanism for PO43--P removal. The sorption process was reversible and the adsorbed PO43--P could be desorbed in both neutral (57-78%) and acidic solution environments (75-88%) for all biochars. Meanwhile, regenerated biochar could re-adsorb up to 5.62 mg/g at the highest initial PO43--P of 150 mg/L. The present finding implied biochar could be effectively used to recover PO43--P from anaerobic digestate.

Pathways of nitrobenzene degradation in horizontal subsurface flow constructed wetlands: Effect of intermittent aeration and glucose addition.

Intermittent aeration and addition of glucose were applied to horizontal subsurface flow constructed wetlands in order to investigate the effect on pathways of nitrobenzene (NB) degradation and interactions with microbial nitrogen and sulphur transformations. The experiment was carried out in three phases A, B and C consisting of different NB loading and glucose dosing. For each phase, the effect of aeration was assessed by intermittently aerating one wetland and leaving one unaerated. Regardless of whether or not the wetland was aerated, at an influent NB concentration of 140 mg/L, both wetlands significantly reduced NB to less than 2 mg/L, a reduction efficiency of 98%. However, once the influent NB concentration was increased to 280 mg/L, the aerated wetland had a higher removal performance 82% compared to that of the unaerated wetland 71%. Addition of glucose further intensified the NB removal to 95% in the aerated wetlands and 92% in the unaerated. Aeration of wetlands enhanced NB degradation, but also resulted in higher NB volatilization of 6 mg m(-2) d(-1). The detected high concentration of sulphide 20-60 mg/L in the unaerated wetland gave a strong indication that NB may act as an electron donor to sulphate-reducing bacteria, but this should be further investigated. Aeration positively improved NB removal in constructed wetlands, but resulted in higher NB volatilization. Glucose addition induced co-metabolism to enhance NB degradation.

Optimization of high-rate TN removal in a novel constructed wetland integrated with microelectrolysis system treating high-strength digestate supernatant.

The potential of high-rate TN removal in three aerated horizontal subsurface-flow constructed wetlands to treat high-strength anaerobic digestate supernatant was evaluated. Different strategies of intermittent aeration and effluent recirculation were applied to compare their effect on nitrogen depuration performance. Additional glucose supply and iron-activated carbon based post-treatment systems were established and examined, respectively, to further remove nitrate that accumulated in the effluents from aerated wetlands. The results showed that intermittent aeration (1 h on:1 h off) significantly improved nitrification with ammonium removal efficiency of 90% (18.1 g/(m(2)·d)), but limited TN removal efficiency (53%). Even though effluent recirculation (a ratio of 1:1) increased TN removal from 53% to 71%, the effluent nitrate concentration was still high. Additional glucose was used as a post-treatment option and further increased the TN removal to 82%; however, this implementation caused additional organic pollution. Furthermore, the iron-activated carbon system stimulated with a microelectrolysis process achieved greater than 85% effluent nitrate removal and resulted in 86% TN removal. Considering the high TN removal rate, aerated constructed wetlands integrated with a microelectrolysis-driven system show great potential for treating high-strength digestate supernatant.

Dynamics of organic matter, nitrogen and phosphorus removal and their interactions in a tidal operated constructed wetland.

This paper demonstrates the potential of tidal flow operated constructed wetland application for the removal dynamics of organic matter, nitrogen and phosphorus. Near-complete removal of organic matter was achieved with a constant removal efficiency of 95%, irrespective of TOC influent loadings ranged from 10 g/m(2) · d to 700 g/m(2) · d. High NH4(+)-N removal at 95% efficiency under influent loading of 17 g/m(2) · d, was stably obtained and was not negatively influenced by increasing influent organic carbon loading rate. Increased influent TOC loading (350 g/m(2) · d to 700 g/m(2) · d) significantly enhanced denitrification capacity and increased TN removal from 30% to 95%. Under tidal flow operation, a higher carbon supply (C/N = 20) for complete TN removal was demonstrated as comparing to that observed in traditional CWs approaches. In addition, the removal of phosphorus was strongly influenced by organic loadings. However, further investigations are needed to elucidate the detailed mechanism that would explain the role of organic loading in phosphorus removal.

Pretreatment of poultry manure anaerobic-digested effluents by electrolysis, centrifugation and autoclaving process for Chlorella vulgaris growth and pollutants removal.

Different pretreatments (electrolysis, centrifugation and autoclaving) coupled with Chlorella vulgaris biological system was used for the treatment of poultry manure anaerobic-digested effluents. The pretreated effluents were used as the growth medium for algal cultivation. The pollutant removal efficiencies of the combined treatments were determined. Electrochemical pretreatment can efficiently remove the ammonia (NH4+), total phosphorus (TP), total organic carbon (TOC), total carbon (TC), turbidity and bacteria in the digested effluents. About 100.0% NH4+, turbidity and bacteria, 97.6% TP, 81.5% TOC and 96.6% inorganic carbon were removed by 5-h electrochemical treatment. The maximal algal biomass accumulation (0.53 g L(-1)) was obtained from culture in the effluents pretreated with 2-h electrolysis. The pollutants removal amounts by the combination of electrolysis and biological treatment were much higher than the other combinations.

Synergistic effect of hydrothermal sludge and food waste in the anaerobic co-digestion process: microbial shift and dewaterability.

While thermal hydrolysis technology is commonly employed for sewage sludge treatment in extensive wastewater treatment facilities, persistent challenges remain, including issues such as ammonia-induced digestive inhibition and reduced productivity stemming from nutrient deficiency within the hydrothermal sludge. In this study, the effects of hydrothermal sludge-to-food waste mixing ratios and fermentation temperatures on anaerobic co-digestion were systematically investigated through a semi-continuous experiment lasting approximately 100 days. The results indicated that anaerobic co-digestion of hydrothermal sludge and food waste proceeded synergistically at any mixing ratio, and the synergistic effect is mainly attributed to the improvement of carbohydrate removal and digestive system stability. However, thermophilic digestion did not improve the anaerobic performance and methane yield. On the contrary, mesophilic digestion performed better in terms of organic matter removal, especially in the utilization of soluble carbohydrates, soluble proteins, and VFAs. Microbial community analysis revealed that the transition from mesophilic to thermophilic anaerobic co-digestion prompts changes in the methane-producing pathways. Specifically, the transition entails a gradual shift from pathways involving acetoclastic and hydrogenotrophic methanogenesis to a singular hydrogenotrophic methanogenesis pathway. This shift is driven by thermodynamic tendencies, as reflected in Gibbs free energy, as well as environmental factors like ammonia nitrogen and volatile fatty acids. Lastly, it is worth noting that the introduction of food waste did lead to a reduction in cake solids following dewatering. Nevertheless, it was observed that thermophilic digestion had a positive impact on dewatering performance.

Effects of Hydrothermal Pretreatment and Anaerobic Digestion of Pig Manure on the Antibiotic Removal and Methane Production.

Whether advanced biological waste treatment technologies, such as hydrothermal pretreatment (HTP) integrated anaerobic digestion (AD), could enhance the removal of different antibiotics remains unclear. This study investigated the outcome of antibiotics and methane productivity during pig manure treatment via HTP, AD, and HTP + AD. Results showed improved removal efficiency of sulfadiazine (SDZ), oxytetracycline (OTC), and enrofloxacin (ENR) with increased HTP temperatures (70, 90, 120, 150, and 170 °C). OTC achieved the highest removal efficiency of 86.8% at 170 °C because of its high sensitivity to heat treatment. For AD, SDZ exhibited resistance with a removal efficiency of 52.8%. However, OTC and ENR could be removed completely within 30 days. When HTP was used prior to AD, OTC and ENR could achieve complete removal. However, residual SDZ levels reduced to 20% and 16% at 150 and 170 °C, respectively. The methanogenic potential showed an overall upward trend as the HTP temperature increased. Microbial analysis revealed the antibiotics-induced enrichment of specific microorganisms during AD. Firmicutes were the dominant bacterial phylum, with their abundance positively correlated with the addition of antibiotics. Methanobacterium and Methanosarcina emerged as the dominant archaea that drove methane production during AD. Thus, HTP can be a potential pretreatment before AD to reduce antibiotic-related risks in manure waste handling.