Breed differences in oral behaviors in feed-restricted dairy heifers.

Holsteins and Jerseys, the 2 most prominent dairy breeds in the United States, differ in many regards. They have not been evaluated for differences in oral behavior performance, despite anecdotal evidence that Jerseys perform more abnormal behaviors than Holsteins. As abnormal behaviors can indicate compromised welfare, we evaluated whether breed differences existed in year-old heifers. Because many oral behaviors could be expressed in abnormal ways, we also sought to describe performance of a wide range of behaviors and whether these varied among individuals. We studied 42 pair-housed heifers (33 Holstein, 9 Jersey) at 12.8 ± 1.1 mo of age (mean ± SD) that were restricted to 50% of their ad libitum total mixed ration (TMR) intake for 2 d as part of a short-term feed challenge. Using continuous video recording from 0800 to 2000 h on the second day of feed restriction, we scored time spent performing tongue rolling, tongue flicking, self-grooming, allogrooming, intersucking, drinking urine, drinking water, and nonnutritive oral manipulation (NNOM) of rice hull bedding, the feed bin, or other pen fixtures. Eating TMR was recorded at 5-min intervals. We found that Jerseys spent more time tongue rolling (3.3% vs. 0.2% proportion of 12-h observations) and performing all types of NNOM than Holsteins (feed bin: 3.8% vs. 2.4%; bedding: 7.7% vs. 5.4%; other: 7.5% vs. 4.2%; total: 19.0% vs. 12.0%), and tended to spend more time tongue flicking (1.4% vs. 1.1%). Jerseys spent less time allogrooming than Holsteins (1.3% vs. 3.4%). There was no evidence of an effect of breed on self-grooming (2.0%), water drinking (1.0%), eating TMR (16.0%), or intersucking (0.06%). Urine drinking was performed by 9 total heifers and was not compared between breeds. All behaviors were highly variable across individuals, particularly tongue rolling and intersucking. Allogrooming was more variable than self-grooming, and each subcategory of NNOM was more variable than total NNOM. Outliers, or extreme performance of oral behaviors relative to the rest of our population, were present in most behaviors. Heifers who were outliers in one behavior were not consistently outliers in all. Overall, there are breed differences in many oral behaviors in a feed-restricted environment. Despite no difference in proportion of time spent eating, Jerseys often performed higher levels of potentially abnormal behaviors than Holsteins, though both breeds performed many oral behaviors, sometimes at extreme levels, that may indicate a concern.

Providing long hay in a novel pipe feeder or a bucket reduces abnormal oral behaviors in milk-fed dairy calves.

Many milk-fed dairy calves are not provided forage. In these settings, calves often perform abnormal repetitive behaviors (ARBs), including tongue rolling and nonnutritive oral manipulation (NNOM), which, based on their form, seem similar to movements used when processing feed. Feeding hay, typically presented as a short chop (≤5 cm) in a bucket, reduces ARBs. Our objective was to evaluate whether altering the presentation method of long hay (∼19 cm), by providing it in a bucket or a novel polyvinyl chloride (PVC) pipe feeder, could reduce ARBs. Holstein heifer calves were housed individually on sand and fed ad libitum starter grain and limited milk replacer (5.7-8.4 L/d step-up) via a bottle (Control, n = 9) or given access to mountaingrass hay in a bucket (Bucket, n = 9) or in a PVC pipe feeder (Pipe, n = 9). The 56 × 10.2 cm (length × diameter) PVC pipe feeder had 4 openings that were 6.35 cm wide, which required the calf to insert her tongue into the pipe and curl her tongue to extract hay. Treatments were applied from birth through 50 d of age, when step-down weaning began and TMR was provided to all calves. Calves were fully weaned at d 60. At wk 4 and 6, oral behaviors (eating, ruminating, drinking water, sucking milk, self-grooming, NNOM, tongue rolling, tongue flicking, and panting) were recorded by direct observation for 24 h using 1-0 sampling during 5-s intervals. Feeding long hay, regardless of presentation method, increased overall DMI, grain intake, and ADG compared with Control calves. Hay provision also increased rumination (25 vs. 15% of 24-h observations in Control) and eating time (5.5 vs. 2% in Control). Abnormal behaviors were seen in all calves. Hay provision reduced some of these, including NNOM (5 vs. 9% in Control). There was no difference in NNOM between calves fed hay in a pipe or bucket, even though Bucket calves consumed more hay (178 vs. 129 g/d in wk 6) and tended to spend more observations eating hay than Pipe calves (4.5 vs. 3%). Hay provision did not affect other behaviors: drinking water (0.5%), grooming (3%), or tongue flicking (3%). We also found evidence of other abnormal oral behaviors that have received less attention. Calves showed signs of polydipsia, and displayed excessive grooming, the latter indicated by overall duration, number of bouts per day, and duration of individual bouts (up to 25 min). Tongue rolling was expressed at low levels (up to 0.4% of intervals) but by 85% of calves. Feeding hay, both in a bucket and using novel methods, was not enough to counteract the welfare challenges associated with individual housing and limited ability to suck milk (<1% of time). Provision of long hay, regardless of presentation method, promotes rumination, improves performance (higher grain intake and ADG) and reduces at least some, but not all, of the considerable abnormal oral behaviors these calves performed.

Early life access to hay does not affect later life oral behavior in feed-restricted heifers.

Dairy cattle are often raised in environments that lack natural feeding opportunities, and they perform abnormal repetitive behaviors (ARBs) as a result. Early life restriction can affect later life behavior. We evaluated whether access to hay in the milk-fed period would affect later life behavior in heifers experiencing short-term feed restriction and whether individuals were consistent in behavioral expression over time. We had 2 competing ideas about how this would unfold. First, being raised with hay, which reduced early life ARBs, could lead to fewer ARBs later in life. Alternatively, heifers that were raised without hay and performed more ARBs in early life might be more prepared for a later feed-restricted environment and thus engage in fewer ARBs than those raised with hay. We studied 24 pair-housed Holstein heifers. As calves, they were fed milk and grain from 0 to 7 wk of age (control) or given additional forage (hay). Tongue rolling, tongue flicking, nonnutritive oral manipulation (NNOM) of pen fixtures, self-grooming, and water drinking were recorded for 12 h (0800-2000 h) during wk 4 and 6 of life using 1-0 sampling at 5-s intervals. At the start of weaning at d 50, all calves were fed a total mixed ration. All calves were fully weaned at d 60 and socially housed by d 65 to 70. After this point, all individuals were raised the same way, according to farm protocol, in groups that included both treatments. At 12.4 ± 0.6 mo of age (mean ± standard deviation), heifers were restricted to 50% of their ad libitum total mixed ration intake for 2 d as part of a short-term feed challenge. Using continuous video recording from 0800 to 2000 h on d 2 of feed restriction, we scored time spent performing oral behaviors: the 5 previously scored while they were calves, along with intersucking, allogrooming, drinking urine, NNOM of rice hull bedding, and NNOM of feed bins. We found that early life access to hay did not affect behavior performed by heifers experiencing short-term feed restriction 1 yr later. Most heifers performed a wide variety of behaviors that appeared abnormal. All heifers performed tongue rolling and NNOM, and at higher levels than when they were calves, while tongue flicks and self-grooming were performed less by heifers. Individual performance of NNOM and tongue rolling were not related across age classes [correlation coefficient (r) = 0.17 and 0.11, respectively], but tongue flicks tended to be correlated (r = 0.37). Intersucking was recorded in 67% of heifers, despite their not being able to suckle a conspecific or dam in early life. Oral behaviors were highly variable across heifers, particularly tongue rolling and intersucking. Outliers, or extreme performance of oral behaviors relative to the rest of the population, were present for many behaviors. Most outliers were expressed by unique heifers that were not extreme in other behaviors. Overall, feeding individually housed, milk-limited calves hay for their first 7 wk did not affect later life performance of oral behaviors. The considerable variability, inconsistency across ages, and excessive performance of some behaviors raises additional questions about how these develop in cattle across life stages and about what we label "abnormal."

Behavioral changes in the first 3 weeks after disbudding in dairy calves.

Hot-iron disbudding, the practice of cauterizing horn bud tissue to prevent horn growth in dairy calves, results in behavioral changes indicative of pain in the first few days after the procedure. However, few studies have quantified behavioral changes in the following weeks, while the burn wounds are still healing. Female Holstein calves were disbudded with a heated iron and pain relief (5.5 mL lidocaine cornual nerve block and 1 mg/kg oral meloxicam) at 4 to 10 d of age (n = 19) or not disbudded (n = 19). Calves wore ear tag accelerometers that reported the dominant behavior being performed at 1-min intervals from 3 to 21 d after disbudding. Compared with age-matched controls, disbudded calves tended to spend more time inactive throughout the observation period, ruminated less in the first 3 to 11 d after disbudding, and sucked more from a milk bottle beginning 5 d after disbudding until the end of the 21-d observation period. In addition to the accelerometer data, live observations of sleeping (using a behavioral proxy), lying, and ruminating were collected using instantaneous sampling at 5-min intervals for 24-h periods 3, 10, and 17 d after disbudding. Disbudded calves slept with their head down more on all live observation days and spent more time lying on the 17th d after disbudding, but ruminating did not differ compared with controls, in contrast to the accelerometer results. More time spent inactive, sleeping, and lying, and less time spent ruminating (as indicated by the accelerometer) can be interpreted as attempts to reduce painful stimulation of the disbudding wounds and allocate energy to healing. It is unclear whether the greater amount of sucking in the disbudded calves is nutritive (milk present) or non-nutritive (milk absent), as the algorithm did not distinguish the type of sucking, and further research is needed to explore the factors underlying this effect. We conclude that disbudding alters daily behavior patterns for at least 3 wk, far beyond the duration of recommended pain medication, raising additional welfare concerns about the procedure.

Limit feeding total mixed rations exacerbates intersucking in year-old dairy heifers.

Limit feeding is a practice that is used to improve feed efficiency and control growth in dairy heifers, but also has negative consequences associated with hunger and restriction of feeding behavior. One such consequence could be intersucking (i.e., the licking or sucking of another animal's teats or udder). This behavior is reported to varying degrees in heifers, and thus, our first objective was to evaluate whether intersucking increased in response to short-term (48 h) feed restriction. Intersucking interventions, such as nose rings, are often applied to specific animals, and so our second objective was to describe intersucking performance in individual heifers, including those that represent outliers in this population. We studied 42 heifers (9 Jerseys, 33 Holsteins) aged 12.8 ± 1.1 mo (mean ± SD). They were housed in 21 pairs, the experimental unit for our first objective. We used a switchback design with 3 periods of 2 d each where heifers were fed ad libitum total mixed ration in the first and third periods (Baseline and Return, respectively), and limit fed to 50% of ad libitum intake in the second period (Restriction). We scored time spent intersucking continuously and eating at 5-min intervals from video recordings from 0800 to 2000 h on 4 d (baseline d 2, restriction d 2, return d 1, and return d 2). Heifer pairs spent less time eating (16 ± 0.4% mean percentage of 12-h observations ± SE), more time intersucking (27 ± 7 s/12 h), and engaged in longer bouts of intersucking (23 ± 5 s/bout) on Restriction d 2 compared with all other days (eating: 34.7 ± 1.1%, 44.2 ± 0.9%, 35.8 ± 1.1% of 12-h; intersucking time: 7 ± 2, 7 ± 3, 10 ± 5 s/12 h; intersucking bout length: 3 ± 1, 2 ± 1, 3 ± 1 s/bout; Baseline d 2, Return d 1, Return d 2, respectively). There was no difference in the number of bouts of intersucking across days (1.3 ± 0.2 bouts/12 h). The drop in eating during feed restriction was followed by a rebound on Return d 1 before returning to baseline levels. Time spent intersucking did not differ among ad libitum periods. Overall, 90% of the heifers performed intersucking on at least 1 of the 4 d, and did this for 1 to 127 s/12 h (range) in 1 to 13 bouts/12 h. Of the heifers that intersucked, 55% did this at extreme levels relative to the rest of the experimental population (outliers). Solid feed restriction exacerbated intersucking in year-old heifers, but this behavior was widespread.

Development and application of a novel approach to scoring ear tag wounds in dairy calves.

Application of ear tags in cattle is a common husbandry practice for identification purposes. Although it is known that ear tag application causes damage, little is known about the duration and process of wound healing associated with this procedure. Our objective was to develop a detailed scoring system and use it to quantify wound healing in dairy calves with plastic identification tags. Calves (n = 33) were ear tagged at 2 d of age, and wound photos were taken weekly until 9 to 22 wk of age. This approach generated 10 to 22 observations per calf that were analyzed using a novel wound scoring system. We developed this system to score the presence or absence of external tissue types related to piercing trauma or mechanical irritation along the top of the tag (impressions, crust, and desquamation) and around the piercing (exudate, crust, tissue growth, and desquamation). Ears were scored as "piercing only" when tissue around the ear tag was intact. We found that impressions, crust, tissue growth, and desquamation were still seen in many calves by 12 wk of age. This suggests that extrinsic factors, such as mechanical disturbance and irritation, may have contributed to prolonged wound healing. Indeed, impressions along the top of tag, likely caused by rubbing against the ear, were observed for nearly the full duration of the study. Further research is warranted to understand ways to improve the ear-tagging process.

Hay provision affects 24-h performance of normal and abnormal oral behaviors in individually housed dairy calves.

Dairy calves often perform abnormal repetitive behaviors (ARBs) including tongue rolling and nonnutritive oral manipulation (NNOM) when opportunities to perform feeding behaviors are restricted. Many US dairy farms limit access to milk, a well-studied risk factor for ARBs. However, farms also commonly do not feed forage to young calves, and the motor patterns of oral ARBs resemble those necessary for acquiring and chewing solid feed. Our objective was to assess how access to hay from birth influenced time engaged in normal and abnormal oral behaviors across 24 h. Holstein heifer calves were housed individually on sand bedding and fed ad libitum water and grain (control, n = 11) or given additional access to hay (hay, n = 11) from birth. Calves were fed 5.7 to 8.4 L/d (step-up) of milk replacer via a teat. At the start of step-down weaning (50 ± 1 d), all calves were given access to a total mixed ration. Feed and water intake were measured daily. Oral behaviors (eating, ruminating, sucking milk, drinking water, panting, grooming, tongue flicking, tongue rolling, and NNOM) were recorded by direct observation at wk 2, 4, 6, and 8 using 1-0 sampling at 1-min intervals for 24 h. Grain, hay, and water intake increased over time in the preweaning period. One polydipsic calf regularly consumed >10 L of water/d. During weaning, hay calves tended to consume increasingly more total mixed ration, significantly more water, and less grain than control calves. Access to hay led to more observations spent eating solid feed (7% vs. 5%, mean percentage of intervals) and ruminating (24% vs. 16%) during the preweaning period compared with calves fed only grain, though control calves appeared to ruminate in absence of forage to re-chew. Rumination occurred, to a large extent, overnight. Hay calves also spent less time self-grooming (12% vs. 14%), tongue flicking (14% vs. 18%), and performing NNOM (17% vs. 21%) than control calves. Although NNOM peaked around milk feedings, all 3 behaviors were performed throughout the day. Tongue rolling was rare across treatments, as was panting, which occurred most frequently around 1400 h. There were no behavioral differences during weaning (wk 8). Overall, we found that hay provision affected most oral behaviors that calves perform; it promoted natural feeding behaviors and reduced abnormal ones, suggesting hay should be provided. We also found that calves performed other behaviors, including polydipsia, repetitive grooming, and apparent sham rumination, that may suggest a degree of abnormality in these behaviors that has not been previously identified. These results highlight the importance of considering all oral behaviors to better understand calf welfare.

Validation of 1-0 and instantaneous sampling for quantifying oral behaviors in milk-fed dairy calves.

Oral behaviors, including feeding, drinking, grooming, and non-nutritive behaviors, are used as indicators of health and welfare in dairy calves, but continuous measurement of these behaviors can be labor intensive. Instantaneous sampling is often used to save labor but has only been validated for feeding behavior in calves. One-zero sampling may be an appropriate strategy well suited to capturing the rapid performance of non-nutritive behaviors. Our objective was to validate 1-0 and instantaneous sampling for measurement of oral behaviors around the time of bottle delivery against true values. Eleven Holstein heifer calves were housed individually, provided water, and fed a diet of starter grain and milk replacer (4.8-5.6 L/d step-up) via a bottle. When calves were 23 ± 7 d old, they were video recorded for 30 min before and after the morning 2.5 ± 0.2 L milk meal, from approximately 0900 to 1000 h. We measured ruminating, eating, drinking water, sucking milk, grooming, non-nutritive oral manipulation, and tongue flicks continuously and with instantaneous and 1-0 sampling at 5-, 10-, 30-, and 60-s intervals. We also examined the effect of instantaneous timing within these intervals. Estimates obtained through subsampling were compared with true values via regression analysis. The subsampling interval was determined to represent true values if the coefficient of determination ≥0.9, slope = 1, intercept = 0, and relative error <10%. Ruminating, drinking water, and eating were not performed by all 11 calves and were not included in the analysis. The proportions of time performing non-nutritive oral manipulation, grooming, and tongue flicks generated by continuous and 1-0 sampling were highly correlated but were consistently overestimated by 1-0 sampling, especially as calves spent more time engaged in these behaviors. Sucking milk was accurately represented at intervals of less than 30 s, likely due to most sucking bouts continuing for at least 150 s at a time and low between-calf variability compared with the other behaviors. Different start times within a given instantaneous interval resulted in wide variance in discrepancies between subsampling and continuous recording for all behaviors. We conclude that around milk feeding, 1-0 sampling is an appropriate choice to represent stimulus-elicited behavior, such as sucking milk in a milk-restricted system. However, time engaged in short, highly variable, or intermittent behaviors is not reliably captured via instantaneous or 1-0 sampling in the hour around bottle delivery.

Promoting public engagement in interdisciplinary biological systems education by leveraging American sports-inspired bracket contests on social media and web.

The complexity of modern biology poses challenges in fostering interdisciplinary understanding, particularly between practicing scientists and the public. Furthermore, scientists often lack formal training in science communication, despite various motivations to engage the public. The science literacy of the public in the biological sciences can also vary across socio-economic and cultural backgrounds. Leveraging popular culture and informal learning practices to promote active learning offers promising avenues to enhance public understanding of biological systems. Organized sports hold collective recognition across various communities and cultures, serving as a means to bring people together. Notably, the NCAA March Madness event holds widespread national and international popularity, presenting an opportunity to laterally apply this concept to promote science communication within STEM and biology education. An educational social media and web-based contest tool was developed integrating NCAA-inspired brackets with animal biological systems concepts. The tool featured tournament-style matchups based on animal biological systems, interesting animal facts, and a voting system, all housed within a user-friendly interface. To encourage regular user access to the tool, graphic designs were developed for all social media posts to aid in visual recruitment to the voting website. Based on online metrics, the use of social media garnered repeat users across both the public and educators. The latter noted the tool's simplicity and informative content. Application of this social media and web-based bracket contest tool, which leverages informal settings for active learning for use in biology education, can foster science communication to engage audiences, improve comprehension, and promote interdisciplinary biology education.

Response to novel feed in dairy calves is affected by prior hay provision and presentation method.

Animals raised in environments that prevent natural foraging opportunities may have difficulty adapting to novelty, such as feeding and management changes. Our objective was to evaluate how early provision and presentation of forage in dairy calves affected response to novel TMR (total mixed ration; grain and alfalfa) at weaning. Holstein heifer calves were housed individually in a covered outdoor hutch with an attached uncovered wire-fenced pen on sand bedding. Calves were fed a diet of starter grain and milk replacer (5.7-8.4L/d step-up) via a bottle (Control, n = 9) or given additional access to mountaingrass hay presented either in a bucket (Bucket, n = 9), or PVC pipe feeder (Pipe, n = 9). Treatments were applied from birth through 50 d of age, when step-down weaning began. All calves had 3 buckets and a pipe feeder provided in their uncovered pen area. On d 50, each calf was briefly blocked inside their hutch. TMR was put in the 3rd bucket that previously contained hay (Bucket) or was empty (Control, Pipe). The calf was released from the hutch and video-recorded for 30 min. Neophobia towards TMR was affected by prior experience with presentation: Bucket calves began eating TMR faster than Pipe and Control (P≤0.012) and showed the fewest number of startle responses (P = 0.004). Intake was similar across groups (P = 0.978), suggesting this apparent neophobia was transient, but Control calves took longer to eat than Bucket (P<0.001) and Pipe (P = 0.070) calves and were less likely to give up on eating to lie down instead. These results suggest that previous experience with hay improves processing ability when presented with novel TMR. Overall, response to a novel feed is affected by both early life experience, such as opportunities to process forage, and the presentation of the feed itself. Calves also appear motivated to access forage, evidenced by transient neophobia, high intake, and persistence in feeding by naïve calves.