A Novel Small-Specimen Planar Biaxial Testing System With Full In-Plane Deformation Control.

Simulations of soft tissues require accurate and robust constitutive models, whose form is derived from carefully designed experimental studies. For such investigations of membranes or thin specimens, planar biaxial systems have been used extensively. Yet, all such systems remain limited in their ability to: (1) fully prescribe in-plane deformation gradient tensor F2D, (2) ensure homogeneity of the applied deformation, and (3) be able to accommodate sufficiently small specimens to ensure a reasonable degree of material homogeneity. To address these issues, we have developed a novel planar biaxial testing device that overcomes these difficulties and is capable of full control of the in-plane deformation gradient tensor F2D and of testing specimens as small as ∼4 mm × âˆ¼4 mm. Individual actuation of the specimen attachment points, combined with a robust real-time feedback control, enabled the device to enforce any arbitrary F2D with a high degree of accuracy and homogeneity. Results from extensive device validation trials and example tissues illustrated the ability of the device to perform as designed and gather data needed for developing and validating constitutive models. Examples included the murine aortic tissues, allowing for investigators to take advantage of the genetic manipulation of murine disease models. These capabilities highlight the potential of the device to serve as a platform for informing and verifying the results of inverse models and for conducting robust, controlled investigation into the biomechanics of very local behaviors of soft tissues and membrane biomaterials.

Fraction-exponential representation of the viscoelastic properties of dentin.

We propose the fraction-exponential description of the viscoelastic properties of dentin. Creep tests are performed on specimens cut from the molar coronal part. Four parameters determining instantaneous and long term Young's moduli as well as the relaxation time are extracted from the experimental data. The same procedure is repeated using the experimental measurements of Jantarat et al (2002) for the specimens cut from the root part of incisor. Physical meaning of the parameters and the difference between them for different sets of specimens are discussed.

Mechanical characterization of Xenopus laevis oocytes using atomic force microscopy.

Mechanical properties are essential for the biological activities of cells, and they have been shown to be affected by diseases. Therefore, accurate mechanical characterization is important for studying the cell lifecycle, cell-cell interactions, and disease diagnosis. While the cytoskeleton and actin cortex are typically the primary structural stiffness contributors in most live cells, oocytes possess an additional extracellular layer known as the vitelline membrane (VM), or envelope, which can significantly impact their overall mechanical properties. In this study, we utilized nanoindentation via an atomic force microscope to measure the Young's modulus of Xenopus laevis oocytes at different force setpoints and explored the influence of the VM by conducting measurements on oocytes with the membrane removed. The findings revealed that the removal of VM led to a significant decrease in the apparent Young's modulus of the oocytes, highlighting the pivotal role of the VM as the main structural component responsible for the oocyte's shape and stiffness. Furthermore, the mechanical behavior of VM was investigated through finite element (FE) simulations of the nanoindentation process. FE simulations with the VM Young's modulus in the range 20-60 MPa resulted in force-displacement curves that closely resemble experimental in terms of shape and maximum force for a given indentation depth.

Elasticity and material anisotropy of lamellar cortical bone in adult bovine tibia characterized via AFM nanoindentation.

The research focuses on the evaluation of the mechanical properties of osteonal cortical bone at the lamellar level. Elastic properties of the mid-diaphysis region of the bovine tibia are investigated via cantilever-based nanoindentation at the submicron length scale utilizing Atomic Force Microscopy, where the force-displacement curves are used for the elastic assessment using the Derjaguin-Muller-Toropov model to calculate indentation modulus. Variations of the modulus and the directional mechanical response of the osteonal bone at different distances from the Haversian canal are investigated. Additionally, the effects of demineralization on the indentation modulus are discussed. It was found that in the axial direction, the first and last untreated thick lamella layers show a significant indentation modulus difference compared to all other layers (4.26 ± 0.4 and 4.6 ± 0.3 GPa vs ∼3.5 GPa). On the other hand, the indentation modulus of transverse thick lamella layers shows a periodic variation between ∼3 ± 0.7 GPa and ∼4 ± 0.3 GPa from near the Haversian canal to near the interstitial bone. A periodic variation in the anisotropy ratio was found. Mineral content was quantified via energy-dispersive X-ray microanalysis at different levels of mineralization and shows a positive correlation with the indentation modulus.