RESUMO
A difference in the base composition of the DNA of Bacterium paracoli 5099 and of a "mutant" (No. 1975) derived from it was found. This is in accordance with the finding of others. However, biochemical tests revealed that the "mutant" was a Flavobacterium, whereas the parent strain belonged to a species of Escherichia. The base composition of the DNA of the "mutant" is similar to that reported for the DNA of Flavobacterium.
Assuntos
DNA Bacteriano/análise , Enterobacteriaceae/classificação , Escherichia coli/classificação , Flavobacterium/classificação , Adenina/análise , Centrifugação com Gradiente de Concentração , Citosina/análise , Genética Microbiana , Guanina/análise , Biologia Molecular , Mutação , Timina/análiseRESUMO
The use of a dip-slide (Uricult) procedure for the culture of voided urine specimens in a large general hospital was found to give equivalent results to those obtained by conventional culture technics. There was a significant decrease in the number of unsatisfactory speciments that needed to be repeated. The dip-slide procedure was readily accepted by physicians, nurses and laboratory personnel, and significant savings for the institution were realized.
Assuntos
Técnicas Bacteriológicas/instrumentação , Bacteriúria/diagnóstico , Bactérias/isolamento & purificação , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/diagnóstico , Humanos , Métodos , Controle de Qualidade , Manejo de EspécimesRESUMO
Two cases of group C streptococcal bacteremia in intravenous drug abusers are described. Both patients had joint involvement and may have been immunocompromised. The literature pertinent to this organism is briefly reviewed.
Assuntos
Infecções Oportunistas/etiologia , Infecções Estreptocócicas/etiologia , Streptococcus/patogenicidade , Transtornos Relacionados ao Uso de Substâncias/complicações , Adulto , Feminino , Humanos , Tolerância Imunológica , Masculino , Infecções Estreptocócicas/imunologiaRESUMO
Useful diagnostic information can be provided by the microbiology laboratory in a variety of infectious diseases. It is important to obtain an appropriate specimen and to provide the laboratory with relevant information. The value and limitations of in vitro susceptibility testing are discussed.
Assuntos
Infecções Bacterianas/diagnóstico , Técnicas Bacteriológicas , Humanos , Testes de Sensibilidade Microbiana , Manejo de EspécimesRESUMO
Some of the Salmonella typhimurium strains in mutagenesis testing are atypical. Unlike the wild S. typhimurium these do not produce H-2-S, are not agglutinated by S. typhimurium typing sera and on fermentation of carbohydrates they do not produce gas. Thus these tester strains cannot readily be identified as S. typhimurium by standard laboratory procedures. This may cause a problem in the differentiation of tester strains from bacterial contaminants. The possibility exits that these phenotypic traits may affect the response of the strains to mutagens.
Assuntos
Mutagênicos/farmacologia , Mutação , Salmonella typhimurium/efeitos dos fármacos , Fenótipo , Salmonella typhimurium/classificação , Salmonella typhimurium/metabolismoAssuntos
Infecções por Bacteroides/complicações , Sepse/etiologia , Idoso , Técnicas Bacteriológicas , Infecções por Bacteroides/tratamento farmacológico , Infecções por Bacteroides/mortalidade , Cloranfenicol/uso terapêutico , Resistência Microbiana a Medicamentos , Feminino , Gastroenteropatias/complicações , Humanos , Canamicina/uso terapêutico , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Sepse/tratamento farmacológico , Sepse/microbiologia , Sepse/mortalidadeAssuntos
Aeromonas , Sepse/etiologia , Aeromonas/isolamento & purificação , Anemia Falciforme/complicações , Anemia Falciforme/microbiologia , Bactérias/isolamento & purificação , Infecções Bacterianas/etiologia , Celulite (Flegmão)/complicações , Celulite (Flegmão)/etiologia , Celulite (Flegmão)/microbiologia , Feminino , Humanos , Pessoa de Meia-Idade , Ferimentos e Lesões/complicaçõesRESUMO
Smallpox represents both the acme of man's efforts to combat infectious diseases and one of his greatest fears. The disease emerged in prehistoric times to spread throughout the world causing blindness and death in millions of people. An acute infection caused by variola virus, one of the Orthopoxviruses, with skin eruption and marked toxemia had an average case fatality rate of 30%. Variola minor, a milder form of the disease, had a case fatality of one percent. Humans are the sole host, and survival confers lifelong immunity. Immunization was practiced since ancient times by inoculation with the variola virus until Jenner's demonstration of the efficacy and safety of vaccination with vaccinia virus. Following an intensive eradication effort by the World Health Organization, the world was declared to be free of smallpox in 1979. The decision to destroy all remaining stocks of variola virus in 1999 has met with some controversy.