Tracing 100 million years of grass genome evolutionary plasticity.

Grasses derive from a family of monocotyledonous plants that includes crops of major economic importance such as wheat, rice, sorghum and barley, sharing a common ancestor some 100 million years ago. The genomic attributes of plant adaptation remain obscure and the consequences of recurrent whole genome duplications (WGD) or polyploidization events, a major force in plant evolution, remain largely speculative. We conducted a comparative analysis of omics data from ten grass species to unveil structural (inversions, fusions, fissions, duplications, substitutions) and regulatory (expression and methylation) basis of genome plasticity, as possible attributes of plant long lasting evolution and adaptation. The present study demonstrates that diverged polyploid lineages sharing a common WGD event often present the same patterns of structural changes and evolutionary dynamics, but these patterns are difficult to generalize across independent WGD events as a result of non-WGD factors such as selection and domestication of crops. Polyploidy is unequivocally linked to the evolutionary success of grasses during the past 100 million years, although it remains difficult to attribute this success to particular genomic consequences of polyploidization, suggesting that polyploids harness the potential of genome duplication, at least partially, in lineage-specific ways. Overall, the present study clearly demonstrates that post-polyploidization reprogramming is more complex than traditionally reported in investigating single species and calls for a critical and comprehensive comparison across independently polyploidized lineages.

Maize plants can enter a standby mode to cope with chilling stress.

BACKGROUND: European Flint maize inbred lines are used as a source of adaptation to cold in most breeding programs in Northern Europe. A deep understanding of their adaptation strategy could thus provide valuable clues for further improvement, which is required in the current context of climate change. We therefore compared six inbreds and two derived Flint x Dent hybrids for their response to one-week at low temperature (10 °C day/7 or 4 °C night) during steady-state vegetative growth. RESULTS: Leaf growth was arrested during chilling treatment but recovered fast upon return to warm temperature, so that no negative effect on shoot biomass was measured. Gene expression analyses of the emerging leaf in the hybrids suggest that plants maintained a 'ready-to-grow' state during chilling since cell cycle genes were not differentially expressed in the division zone and genes coding for expansins were on the opposite up-regulated in the elongation zone. In photosynthetic tissues, a strong reduction in PSII efficiency was measured. Chilling repressed chlorophyll biosynthesis; we detected accumulation of the precursor geranylgeranyl chlorophyll a and down-regulation of GERANYLGERANYL REDUCTASE (GGR) in mature leaf tissues. Excess light energy was mostly dissipated through fluorescence and constitutive thermal dissipation processes, rather than by light-regulated thermal dissipation. Consistently, only weak clues of xanthophyll cycle activation were found. CO2 assimilation was reduced by chilling, as well as the expression levels of genes encoding phosphoenolpyruvate carboxylase (PEPC), pyruvate orthophosphate dikinase (PPDK), and the small subunit of Rubisco. Accumulation of sugars was correlated with a strong decrease of the specific leaf area (SLA). CONCLUSIONS: Altogether, our study reveals good tolerance of the photosynthetic machinery of Northern European maize to chilling and suggests that growth arrest might be their strategy for fast recovery after a mild stress.

Analyzing Changes in Maize Leaves Orientation due to GxExM Using an Automatic Method from RGB Images.

The sowing pattern has an important impact on light interception efficiency in maize by determining the spatial distribution of leaves within the canopy. Leaves orientation is an important architectural trait determining maize canopies light interception. Previous studies have indicated how maize genotypes may adapt leaves orientation to avoid mutual shading with neighboring plants as a plastic response to intraspecific competition. The goal of the present study is 2-fold: firstly, to propose and validate an automatic algorithm (Automatic Leaf Azimuth Estimation from Midrib detection [ALAEM]) based on leaves midrib detection in vertical red green blue (RGB) images to describe leaves orientation at the canopy level; and secondly, to describe genotypic and environmental differences in leaves orientation in a panel of 5 maize hybrids sowing at 2 densities (6 and 12 plants.m-2) and 2 row spacing (0.4 and 0.8 m) over 2 different sites in southern France. The ALAEM algorithm was validated against in situ annotations of leaves orientation, showing a satisfactory agreement (root mean square [RMSE] error = 0.1, R2 = 0.35) in the proportion of leaves oriented perpendicular to rows direction across sowing patterns, genotypes, and sites. The results from ALAEM permitted to identify significant differences in leaves orientation associated to leaves intraspecific competition. In both experiments, a progressive increase in the proportion of leaves oriented perpendicular to the row is observed when the rectangularity of the sowing pattern increases from 1 (6 plants.m-2, 0.4 m row spacing) towards 8 (12 plants.m-2, 0.8 m row spacing). Significant differences among the 5 cultivars were found, with 2 hybrids exhibiting, systematically, a more plastic behavior with a significantly higher proportion of leaves oriented perpendicularly to avoid overlapping with neighbor plants at high rectangularity. Differences in leaves orientation were also found between experiments in a squared sowing pattern (6 plants.m-2, 0.4 m row spacing), indicating a possible contribution of illumination conditions inducing a preferential orientation toward east-west direction when intraspecific competition is low.