Decomposition of maize stover varies with maize type and stover management strategies: A microcosm study on a Black soil (Mollisol) in northeast China.

Crop residue decomposition has an important impact on soil organic carbon (SOC) sequestration and CO2 emission. Residue quality and management strategies are two important factors regulating decomposition process and SOC mineralization and greenhouse gas emission. In this study, a microcosm experiment in field condition was conducted on a silty loam (a Black soil) in Northeast China to investigate stover decomposition and soil CO2 emission characteristics as influenced by different crop cultivars and stover field incorporation methods. Stover from two popular maize cultivars Xianyu335 (XY) and Liangyu99 (LY) were applied in two modes (soil surface application vs soil incorporation) at a rate of 11 t ha-1, and CO2 efflux was monitored during the decomposition duration of 144 days. The structural transformation of carbon functional groups in maize stover were evaluated using solid state 13C-CPMAS NMR and elemental analysis techniques. Results showed that up to 71.7%∼86.9% (weight basis) of C and N in soil-incorporated stover was decomposed during the study period, which was significantly greater than the losses (32.8%∼55.3%) of C or N from the surface-applied stover for both maize cultivars; decomposition rates of main C functional groups were significantly higher in soil incorporation (71.1%∼88.8%) than in surface application (20.9%∼60.2%) systems. The concentrations of SOC, total N, available N, and microbial biomass C and N in soil were also higher with stover incorporation than surface application. Stover incorporation resulted in a notably lower CO2 emission rate and accumulative CO2 efflux (53.9-55.4 mol m-2) during the stover decomposition compared with surface application (57.4-67.0 mol m-2). Between the two maize cultivars, the LY stover showed a higher decomposition rate and greater capacity for SOC sequestration when incorporated into soil. The LY stover induced higher (16.8%) CO2 emission than XY when applied on soil surface, but no significant difference was found between the two cultivars when incorporated into soil. The results suggested that cultivar selection and stover management strategies have great potential in reducing soil CO2 emission while improving soil biochemical properties. Incorporating the LY stover into soil rather than surface mulching could enhance SOC sequestration and reduce CO2 emission.

[Assessment of humic and fulvic acids in black soils using near-infrared reflectance spectroscopy].

The organic carbon content and optical densities of humic acids in black soils of China were predicted and assessed using near infrared spectroscopy technique. The contents of humic acid (HA) and fulvic acid (FA) in 136 black soil samples in China were analyzed and the NIR spectra were collected using a VECTOR/22 (Fourier transform infrared spectroscopy). Partial least squares (PLS) regression with cross validation was used to develop prediction models with reference data and soil NIRS spectra, and the model was validated using an independent set of samples. NIRS well predicted (HAC+FAC), HAC and FAC contents, with R2 = 0.92, 0.92 and 0.86, RPD = 3.66, 3.82 and 2.69, and high correlation coefficients between predicted and measured values (r = 0.90, 0.85 and 0.82). Predictions for the E4 values of HA and FA were also good (R2 = 0.85, 0.85; RPD = 2.88, 2.65; r = 0.92, 0.80). Predictions for optical densities of HA and FA at 665 nm (E6) was acceptable. Generally, NIRS showed a good potential to predict C content and optical densities of humic acid and fulvic acid in blacks soils and may reveal information on SOC quality.

[Prediction of soil organic carbon in different soil fractions of black soils in Northeast China using near-infrared reflectance spectroscopy].

The soil organic carbon (SOC) associated with different soil fractions varies in the composition and dynamics. The present work is aimed to evaluate the potential of near infrared spectroscopy (NIRS) to predict SOC content in different soil fractions of black soils. SOC contents of 136 black soil samples in China were analyzed and the NIR spectra were collected using a VECTOR/22 (Fourier transform infrared spectroscopy). Partial least squares (PLS) regression with cross validation was used to develop calibrations between reference data and NIRS spectra (n = 100) which were validated using an independent set of samples (n = 36). Predictions for water-sieved aggregate associated organic carbon were generally good with R2 (coefficient of determination) ranging from 0.69 to 0.82 and the RPD (residual prediction deviation) from 1.2 to 1.8. NIRS well predicted the SOC in < 53 microm mineral fraction (R2 = 0.97, RPD = 5.4), but the prediction for SOC in 250-2 000 microm or in 53-250 microm particulate matter fractions was poor. However, the prediction for the SOC in 53-2 000 microm fraction was good (R2 = 0.79, RPD = 2.2). In addition, NIRS very well predicted the SOC in fine particle fraction (< 20 microm) (R2 = 0.93, RPD = 3.8). Accordingly, NIRS showed a good potential to predict SOC in some soil fractions and could reduce tedious laboratory analysis.

Influence of Mineral Fertilizer and Manure Application on the Yield and Quality of Maize in Relation to Intercropping in the Southeast Republic of Kazakhstan.

Maize (Zea mays L.) is a valuable forage crop. It is also an essential and promising crop for the Republic of Kazakhstan, cultivated in the southern zone. Some new maize hybrids have been introduced, which have been beneficial for high yields with less fertilizer input. This study aims to introduce the new maize hybrid, Arman 689, for the judicial use of fertilizer and the high yield. This study was carried out in 2015 in the southeast region of Kazakhstan. There are five treatments with various mineral fertilizer and poultry manure doses: 1. control (T0), 2. P60 K100 (T1), 3. N100P60K100 (T2). 4. N100P60K100 + 40 tons of manure/ha (T3), and 5. N100P60K100 + 60 ton of manure/ha (T4). The fertilizers used were ammonium nitrate (N­34.6%), amorphous (N­11.0%, P2O5­46.0%), and potassium chloride KCl (K2O­56%). The results showed that the grain yield ranges from 5.51 t/ha (T0) to 8.49 (T4) t/ha. The protein contents in the maize grain varied from 9%(T0)−11.3%(T4). The grain nitrogen content accounted for 54.2 to 52.0%. The nutrient uptake results by different treatments indicated that nitrogen contributed to 41.5% of the total yield increase. Using manure in combination with mineral fertilizers reduced the payback of the applied resources, as the payback of T2−T4 was 8.8−9.1 kg of grain. With the application of recommended mineral fertilizer (NPK), the protein yield was 0.83 t/ha, 0.33, and 1.22 t/ha higher than T0 and T1 treatments, respectively. There was no significant yield difference under T3 and T4 treatments (p > 0.05). Overall, the treatment, NPK + 40 tons of manure, was proved the ultimate for the Arman hybrid in providing the optimum quantity and quality of maize, as well as reducing the payback cost (8.8−9.1 kg of grain). It is suggested to apply NPK-recommended doses along with manure in maize (Arman hybrid)-based intercropping systems to utilize the resources efficiently.

Straw-derived biochar mitigates CO2 emission through changes in soil pore structure in a wheat-rice rotation system.

To better understand the relationships between soil pore structure features and soil CO2 emission and soil organic carbon (SOC) sequestration following different straw return modes, undisturbed soil cores (0-5 cm and 5-10 cm) were collected from a rice-wheat rotation system under 4 straw return treatments as (1) no straw return (CK), (2) straw direct return (DR), (3) straw biochar return (BR); (4) straw-pig manure fermentation return (FR) for six years. Pore structure parameters including pore size distribution, porosity, connectivity, anisotropy and fractal dimension (FD) were determined using X-ray computer tomography. Soil CO2 flux and concentrations of SOC, readily oxidable carbon and nutrients were also measured. The results showed that BR and FR had significantly higher SOC concentration than DR and CK. Porosity and number of >500 µm and 500-100 µm macropores, FD and connectivity were significantly highest under FR and was lowest under BR. FR and DR produced 28.1%-32.4% higher C-CO2 than CK and BR in wheat growing season, and 9.80%-16.9% higher in rice season. Soil CO2 emission and C concentrations were significantly related to soil pore structure parameters. The CO2 emission was most significantly related to number of >500 µm pores and FD, indicating that poorly developed pore structure under BR hindered the production and diffusion of CO2 from soil. These results enhanced our understanding of the relationship between soil pore structure and CO2 emission following biochar application, and provided evidence for decision making process in choosing proper straw managements to promote SOC sequestration and reduce CO2 emission.

Tillage-induced effects on SOC through changes in aggregate stability and soil pore structure.

Soil structure plays a key role in soil organic carbon (SOC) dynamics. To determine how soil structure and aggregate affects SOC, we collected undisturbed soil cores of 0-5 cm layer (Typic Hapludoll) at an experimental site in Northeast China. The site had been under continuous tillage treatments of conventional tillage (CT) and no tillage (NT) for 17 years. We measured SOC by elemental analysis, aggregate size distribution by wet sieving, and soil pore parameters of pore size distribution, pore average diameter, pore numbers, pore connectivity, pore anisotropy, and pore fractal dimension by X-ray computer tomography. SOC content was significantly correlated with aggregate-associated SOC and soil water-stable aggregate content. CT with residue removal and annual plowing and cultivation increased <53 µm and 53-250 µm aggregates. CT decreased total SOC of 0-5 cm soil layer but increased aggregate-associated SOC of <53 µm. NT with greater residue input increased total SOC of 0-5 cm soil layer by 26.0% and aggregate mean weight diameter by 111.8% and increased aggregates of 250-1000 µm and >1000 µm. Soil under NT had a greater total number of micropores and greater connectivity whereas CT had a greater total number of macropores, average macropore diameter, anisotropy, and fractal dimension. Structural equation modeling showed that CT can decrease SOC of 0-5 cm soil layer by different paths, including increased anisotropy and macropore porosity, and NT can increase SOC of 0-5 cm soil layer by different paths, including increased mean weight diameter and connectivity. These results enhance our understanding of the relationship between soil structure and SOC, and could guide tillage management decisions to increase SOC.

Curve-fitting techniques improve the mid-infrared analysis of soil organic carbon: a case study for Brookston clay loam particle-size fractions.

Few studies have evaluated structural features of soil organic carbon (SOC) in different soil particle fractions, especially SOC changes induced by tillage, using Fourier transformed mid-infrared (MIR) spectroscopy. To make a contribution in this context, soil samples of a Brookston clay loam (mesic Typic Argiaquoll) with averaged pH and organic matter concentration at 7.28 and 43.9 g kg-1, respectively, were collected from short-term no-tillage (NT97) and mouldboard plow (CT97) treatments initiated in 1997 and long-term no-tillage (NT83) and mouldboard plow (CT83) treatments initiated in 1983 under a corn-soybean rotation, and were separated into sand, silt, and clay fractions using sonication. Structural features of SOC in these soil fractions were investigated using curve-fitting analysis of mid-infrared (MIR) spectra. Aromatic C content was found to be greater in clay- than in sand-sized fractions, while aliphatic C content was higher in sand- than in silt- and clay-sized particles. With decrease in tillage intensity, the aliphatic C gradually increased in sand- and clay-sized fractions but not in the silt-sized fraction. The aliphatic C content in sand fraction was significantly greater in NT83 than CT83 (P < 0.05). The aromatic C in silt- and clay-sized fractions was greater in NT83 than in both CT soils, whereas aromatic C contents were higher in both CT soils than in NT83 soil. Significantly higher aromatic/aliphatic C ratio in CT83 than NT83 was found in sand-sized fractions, while the opposite trend was found in the silt-sized fraction. These findings were not apparent until the curve-fitting technique was employed, which has the capacity to quantify many overlapped bands in the spectra. This study demonstrates that the curve-fitting of MIR spectra advances the analysis of organic matter in soil samples.

The Presence of Cu Facilitates Adsorption of Tetracycline (TC) onto Water Hyacinth Roots.

Batch experiments were conducted to investigate the adsorption characteristics of tetracycline (TC), and the interactive effects of copper (Cu) on the adsorption of TC onto water hyacinth roots. TC removal efficiency by water hyacinth roots was ranging from 58.9% to 84.6%, for virgin TC, 1:1 TC-Cu and 1:2 TC-Cu. The Freundlich isotherm model and the pseudo-second-order kinetic model fitted the adsorption data well. Thermodynamics parameters ΔG° for TC were more negative in the TC plus Cu than the TC-only treatments, indicating the spontaneity of TC adsorption increased with increasing of Cu concentrations. An elevated temperature was associated with increasing adsorption of TC by water hyacinth roots. The additions of Cu(II) significantly increased TC adsorption onto water hyacinth roots within the pH range 4 to 6, because copper formed a strong metal bridge between root surface and TC molecule, facilitating the adsorption of TC by roots. However, Cu(II) hindered TC adsorption onto water hyacinth roots on the whole at pH range from 6⁻10, since the stronger electrostatic repulsion and formation of CuOH⁺ and Cu(OH)2. Therefore, the interaction between Cu(II) and TC under different environmental conditions should be taken into account to understand the environmental behavior, fate, and ecotoxicity of TC.

Dynamics of copper and tetracyclines during composting of water hyacinth biomass amended with peat or pig manure.

Composting is one of the post-treatment methods for phytoremediation plants. Due to a high potential of water hyacinth to accumulate pollutants, the physicochemical parameters, microbial activity as well as fates of copper (Cu) and tetracyclines (TCs) were investigated for the different amended water hyacinth biomass harvested from intensive livestock and poultry wastewater, including unamended water hyacinth (W), water hyacinth amended with peat (WP), and water hyacinth amended with pig manure (WPM) during the composting process. Pig manure application accelerated the composting process as evidenced by an increase of temperature, electrical conductivity (EC), NH4-N, as well as functional diversity of microbial communities compared to W and WP treatments. Composting process was slowed down by high Cu, but not by TCs. The addition of peat significantly increased the residual fraction of Cu, while pig manure addition increased available Cu concentration in the final compost. Cu could be effectively transformed into low available (oxidizable) and residual fractions after fermentation. In contrast, less than 0.5% of initial concentrations of TCs were determined at the end of 60-day composting for all treatments in the final composts. The dissipation of TCs was accelerated by the high Cu concentration during composting. Therefore, composting is an effective method for the post-treatment and resource utilization of phytoremediation plants containing Cu and/or TCs.

[Effects of tillage mode on black soil's penetration resistance and bulk density].

Taking an eight-year field experiment site in Dehui County of Jilin Province, Northeast China as test object, this paper studied the effects of different tillage modes (no tillage and ploughing in autumn) on the penetration resistance and bulk density of black soil. No tillage increased the soil penetration resistance, especially at the soil depth of 2.5-17.5 cm. In the continuous cropping of maize and the rotation of maize-soybean, the maximum soil penetration resistance at planting zone under no tillage and ploughing in autumn was 2816 and 1931 kPa, and 2660 and 2051 kPa, respectively, which had no restriction on the crop growth. The curve of soil penetration resistance under ploughing in autumn changed with ridge shape, while that under no tillage changed less. Comparing with ploughing in autumn, no tillage increased the bulk density of 5-20 cm soil layer significantly. Under no tillage, the bulk density of 5-30 cm soil layer changed little, but under ploughing in autumn, soil bulk density increased gradually with increasing soil depth. There was no significant correlation between soil bulk density and soil penetration resistance.

[Near infrared spectroscopy in determining organic carbon and total nitrogen in black soil of Northeast China].

In this study, near infrared reflectance spectroscopy (NIRS) was used to determine the organic carbon (OC), total nitrogen (TN), and C/N ratio in black soil of Northeast China. Based on the 3699-12000 cm(-1) NIRS of 136 black soil samples collected in 2004-2005, and by using partial least square (PLS), the related quantitative models were established. Leave-one-out cross validation showed that the OC and TN were well predicted, with the values of coefficient of determination (R2) being 0.92 and 0.91, RPD (the ratio of standard deviation of validation set to root mean square error of cross validation) being 3.45 and 3.36, and correlation coefficient (r) being 0.94 and 0.93 respectively, suggesting that NIRS had the potential to predict the OC and TN in black soil of Northeastern China. However, the C/N ratio was poorly predicted, with R2 = 0.61, RPD = 1.61, and r = 0.74, indicating that NIRS could not give reasonable prediction for the C/N ratio in black soil.

[Field sampling methods for earthworm: a review].

As a critical component of soil ecosystem, earthworm can improve soil structure and relates closely to soil nutrient cycling, playing an important role in promoting soil quality and productivity. However, there is lack of systematic study on the field sampling methods for earthworm, especially in China. This paper reviewed the operational processes of commonly used field sampling methods for earthworm, and discussed their corresponding merits, efficacy, and potential influence on research results. To achieve a complete and accurate characterization of earthworm community size and structure, the method of chemical repellent combined with hand-sorting could work well at the sites where physical disturbance was acceptable, while the AITC (allyl isothiocyanate) method would be a favorable option at the sites where soil destruction was not feasible.