RESUMO
Studies were undertaken to find a substance or substances for use in primary binding types of tests to detect humoral antibodies in rabbits and monkeys exposed to the tubercle bacillus that would distinguish between strains of mycobacteria. The antigen employed was a component of the 5159 strain of Mycobacterium tuberculosis that was obtained following sonification, ultracentrifugation, electrophoresis, and elution from a preparative polyacrylamide column. When the antigen was labeled with (131)I, specific binding was observed in sera from immunized rabbits and BCG-protected rhesus monkeys by the radio-gel electrophoresis and ammonium sulfate tests. This component was partially characterized, and its major antigenic determinants were associated with anionic mucopolysaccharides. Electrophoretically at pH 8.3 it migrated anodally to albumin, its molecular weight was between 9,000 and 12,000, and it was soluble in 50% saturated ammonium sulfate. Binding to antibody was destroyed after treatment with Pronase, but not after DNase or RNase. Inhibition of the reaction, as measured by the ammonium sulfate test, between the (131)I-labeled component and antisera from rabbits that had been immunized with sonicated 5159 organisms, was studied. These experiments demonstrated a capacity to define subtle similarities and differences among different mycobacteria and mycobacterial components. Some microorganisms not clearly related to mycobacteria also partially inhibited this reaction, suggesting that they shared antigenic groups with the component derived from 5159 organisms. The studies described suggested the advisability of using direct primary tests and purified components of mycobacteria to differentiate further the antigenic groups between individual pathogenic mycobacteria and between pathogenic and nonpathogenic organisms.
Assuntos
Anticorpos/análise , Reações Antígeno-Anticorpo , Antígenos/classificação , Mycobacterium tuberculosis/imunologia , Animais , Antígenos/análise , Bovinos , Eletroforese , Cobaias , Haplorrinos , Imunodifusão , Imunoeletroforese , Isótopos de Iodo , Mycobacterium tuberculosis/classificação , Coelhos , Ultracentrifugação , UltrassomRESUMO
Five human sera with reaginic activity to a number of allergens were fractionated using anion exchange chromatography. In each serum, fractions which contained detectable IgG and no detectable IgA had capacity to fix to skin and subsequently elicit a P-K reaction. Four of these sera had reaginic activity about equally distributed between fractions containing only IgG and fractions containing mixtures of IgG and IgA. A fifth serum contained reaginic activity to crystalline bovine serum albumin (BSA) and most of the activity was associated with the fraction which contained only IgG. This serum was extensively studied using a variety of techniques and it was confirmed that most of the reagin to BSA in this serum was in those fractions containing only IgG. Since reaginic activity can no longer be considered a unique property of IgA the implications of finding antibody with reaginic qualities in immunoglobulins other than IgA are discussed.
Assuntos
Anticorpos , Reações Antígeno-Anticorpo , gama-Globulinas , Asma , Sangue , Poeira , Imunoglobulina E , Técnicas In Vitro , Pólen , Rinite Alérgica Sazonal , Soroalbumina BovinaRESUMO
Human serum albumin is acetylated when exposed to acetylsalicylic acid (aspirin) under physiologic conditions in vitro. Indications are that a similar phenomenon occurs in vivo after ingestion of aspirin.
Assuntos
Acetatos , Alquilação , Aspirina , Albumina Sérica , Ácido Acetrizoico , Animais , Aspirina/efeitos adversos , Asma/induzido quimicamente , Autorradiografia , Isótopos de Carbono , Fenômenos Químicos , Química , Diálise , Hipersensibilidade a Drogas , Humanos , Concentração de Íons de Hidrogênio , Imunoeletroforese , Isótopos de Iodo , Pólipos Nasais/induzido quimicamente , Coelhos , Rinite/induzido quimicamenteRESUMO
Antibody to DNA in human serums can be detected by the ammonium sulfate method. This sensitive and specific technique, which measures the primary interaction between DNA and antibody to DNA, is based on the observation that free DNA is soluble in 50-percent saturated ammonium sulfate whereas antibody-bound DNA is insoluble.
Assuntos
Anticorpos , Reações Antígeno-Anticorpo , DNA , Lúpus Eritematoso Sistêmico/imunologia , Animais , Bacillus subtilis , Bovinos , Precipitação Química , Testes de Fixação de Complemento , DNA Bacteriano , Testes de Hemaglutinação , Heparina , Temperatura Alta , Humanos , Ácido Hialurônico , Métodos , Desnaturação de Ácido Nucleico , Compostos de Amônio Quaternário , RNA , Sulfatos , Timo , Trítio , LevedurasRESUMO
Antigens from Mycobacterium bovis and Listeria monocytogenes bind to serums from normal rabbits as well as from those immunized with unrelated bacteria, especially intracellular parasites. Binding is to the Fab portion of immunoglobulin G and is immunologically specific.
Assuntos
Antígenos/análise , Bactérias/imunologia , Reações Cruzadas , Mycobacterium bovis/imunologia , Albuminas , Animais , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Antígenos de Bactérias/análise , Sítios de Ligação , Brucella abortus/imunologia , Escherichia coli/imunologia , Adjuvante de Freund , Imunização , Isótopos de Iodo , Listeria monocytogenes/imunologia , Neisseria/imunologia , Coelhos , Salmonella typhimurium/imunologia , Staphylococcus/imunologiaRESUMO
Acetylsalicylic acid (aspirin) acetylates human serum albumin under physiologic conditions in vitro. These investigations were done to determine whether this phenomenon occurs in vivo and to delineate the site(s) of acetylation on the albumin molecule. Albumin was reacted in vitro with aspirin labeled with (14)carbon at the acetyl-1 or the carboxyl carbon. The altered albumin was hydrolyzed with trypsin and peptide mapping performed. Albumin so treated contains a unique peptide, designated "A," and shows diminution of two normal peptides, designated "B" and "C." Peptide "A" is never seen in normal albumin. Amino acid analyses indicate that peptide "A" equals the sum of peptides "B" and "C." Furthermore all three peptides contain lysine but lack arginine. Thus peptide "A" is formed by the acetylation of a lysine residue which is normally susceptible to trypsin and yields peptides "B" and "C." Radioautography of the peptide maps show most of the acetyl-1-(14)C activity in peptide "A." This indicates that one of the lysine residues in this peptide is the preferential site for the transacetylation reaction. Peptide "A," used as a marker for acetylation, is found in albumin from patients who take aspirin but is not demonstrable in albumin from one of these patients while she was taking sodium salicylate. A transacetylation reaction between aspirin and human albumin occurs in vivo and is similar to that observed in vitro.
Assuntos
Aspirina/farmacologia , Albumina Sérica/análise , Albumina Sérica/metabolismo , Aminoácidos/análise , Autorradiografia , Isótopos de Carbono , Fenômenos Químicos , Físico-Química , Humanos , Técnicas In Vitro , Biossíntese PeptídicaRESUMO
Innovation is to organizations what evolution is to organisms: it is how organizations adapt to environmental change and improve. Yet despite advances in our understanding of evolution, what drives innovation remains elusive. On the one hand, organizations invest heavily in systematic strategies to accelerate innovation. On the other, historical analysis and individual experience suggest that serendipity plays a significant role. To unify these perspectives, we analysed the mathematics of innovation as a search for designs across a universe of component building blocks. We tested our insights using data from language, gastronomy and technology. By measuring the number of makeable designs as we acquire components, we observed that the relative usefulness of different components can cross over time. When these crossovers are unanticipated, they appear to be the result of serendipity. But when we can predict crossovers in advance, they offer opportunities to strategically increase the growth of the product space.
RESUMO
We adapt a recent theory for the random close packing of polydisperse spheres in three dimensions [R. S. Farr and R. D. Groot, J. Chem. Phys. 131, 244104 (2009)] in order to predict the Hausdorff dimension dA of the Apollonian gasket in dimensions 2 and above. Our approximate results agree with published values in two and three dimensions to within 0.05% and 0.6%, respectively, and we provide predictions for dimensions 4-8.
Assuntos
Adenoviridae/isolamento & purificação , Asma/imunologia , Seio Frontal/microbiologia , Síndromes de Imunodeficiência , Seio Maxilar/microbiologia , Infecções por Adenoviridae/imunologia , Adulto , Animais , Formação de Anticorpos , Doença Crônica , Seio Frontal/patologia , Haplorrinos , Histocompatibilidade , Humanos , Imunidade Celular , Imunização , Síndromes de Imunodeficiência/imunologia , Síndromes de Imunodeficiência/microbiologia , Influenza Humana/imunologia , Ativação Linfocitária , Linfócitos/imunologia , Masculino , Seio Maxilar/patologia , Sinusite/imunologia , Cultura de VírusAssuntos
Anticorpos Antibacterianos , Anticorpos Antifúngicos , Anticorpos Antibacterianos/análise , Anticorpos Antifúngicos/análise , Anticorpos Antineoplásicos/biossíntese , Especificidade de Anticorpos , Infecções Bacterianas/imunologia , Humanos , Mycobacterium bovis/imunologia , Neoplasias/imunologiaAssuntos
Asma/tratamento farmacológico , Broncodilatadores/uso terapêutico , Fentolamina/uso terapêutico , Administração Oral , Asma/fisiopatologia , Atropina/uso terapêutico , Pressão Sanguínea , Espasmo Brônquico/induzido quimicamente , Clorfeniramina/uso terapêutico , Ensaios Clínicos como Assunto , Cromolina Sódica/uso terapêutico , Eletrocardiografia , Efedrina/uso terapêutico , Epinefrina/efeitos adversos , Feminino , Humanos , Isoproterenol/efeitos adversos , Pessoa de Meia-Idade , Fentolamina/administração & dosagem , Esforço Físico , Placebos , Pletismografia Total , Prednisona/uso terapêutico , Espirometria , Fatores de Tempo , Xantinas/uso terapêuticoAssuntos
Anticorpos/análise , Aglutinação , Animais , Reações Antígeno-Anticorpo , Precipitação Química , Proteínas do Sistema Complemento , Eletroforese , Imunofluorescência , Hemólise , Humanos , Soros Imunes , Imunoeletroforese , Isótopos de Iodo , Métodos , Infecções por Mycobacterium/imunologia , Testes de Precipitina , Compostos de Amônio Quaternário , Coelhos , Sulfatos , UltracentrifugaçãoAssuntos
Ácido Acetrizoico/sangue , Aspirina/farmacologia , Ligação Proteica/efeitos dos fármacos , Albumina Sérica/metabolismo , Acetilação , Autorradiografia , Sítios de Ligação , Eletroforese das Proteínas Sanguíneas , Radioisótopos de Carbono , Cromatografia em Camada Fina , Diálise , Interações Medicamentosas , Meia-Vida , Humanos , Concentração de Íons de Hidrogênio , Radioisótopos do Iodo , Peptídeos/sangue , Salicilatos/farmacologiaAssuntos
Asma/diagnóstico , Brônquios/fisiopatologia , Respiração , Adolescente , Adulto , Idoso , Antígenos , Soluções Tampão , Doença Crônica , Poeira , Histamina , Humanos , Compostos de Metacolina , Métodos , Pessoa de Meia-Idade , Poaceae , Pólen , Testes Cutâneos , Espirometria , ÁrvoresAssuntos
Hipersensibilidade/terapia , Assistência de Longa Duração , Anti-Infecciosos/uso terapêutico , Reações Antígeno-Anticorpo , Broncodilatadores/uso terapêutico , Pré-Escolar , Efedrina/uso terapêutico , Epinefrina/uso terapêutico , Expectorantes/uso terapêutico , Glucocorticoides/uso terapêutico , Antagonistas dos Receptores Histamínicos H1/uso terapêutico , Humanos , Hipersensibilidade/tratamento farmacológico , Hipnóticos e Sedativos/uso terapêutico , Imunidade , Testes Cutâneos , Simpatomiméticos/uso terapêutico , Tranquilizantes/uso terapêutico , Xantinas/uso terapêuticoRESUMO
The Scientific Board of the California Medical Association presents the following inventory of items of progress in allergy. Each item, in the judgment of a panel of knowledgeable physicians, has recently become reasonably firmly established, both as to scientific fact and important clinical significance. The items are presented in simple epitome and an authoritative reference, both to the item itself and to the subject as a whole, is generally given for those who may be unfamiliar with a particular item. The purpose is to assist busy practitioners, students, research workers or scholars to stay abreast of these items of progress in allergy that have recently achieved a substantial degree of authoritative acceptance, whether in their own field of special interest or another.The items of progress listed below were selected by the Advisory Panel to the Section on Allergy of the California Medical Association and the summaries were prepared under its direction.
RESUMO
Heterogeneity is the hallmark of adult asthma, and patients thus need individualized therapeutic programs. But there are unifying underlying mechanisms, understanding of which makes it easier to design the clinical stategy appropriate to each case.
Assuntos
Asma , Broncodilatadores/uso terapêutico , Planejamento de Assistência ao Paciente , Obstrução das Vias Respiratórias/etiologia , Assistência Ambulatorial , Asma/diagnóstico , Asma/etiologia , Asma/terapia , Broncodilatadores/administração & dosagem , Volume Expiratório Forçado , Humanos , Transtornos Psicofisiológicos/complicações , Hipersensibilidade Respiratória/complicações , Testes Cutâneos , Esteroides/uso terapêuticoRESUMO
Some patients with asthma cope with their disease in ways that are deleterious to them. The coping styles used by these patients often were used by them before the onset of the asthma, but the asthma amplifies these styles, and the coping styles can amplify the asthma. There are striking similarities between these coping styles and those described in cancer victims. Like the latter, they may reflect denial, anger, bargaining, and depression. Recognition that patients are using these coping styles and appropriate intervention will prevent them from continuing to act in these self-destructive manners.