Pollination features and floral volatiles of Gymnospermium scipetarum (Berberidaceae).

The discovery of few isolated populations of Gymnospermium scipetarum (since now considered as an amphi-Adriatic endemic) in the S-Apennines prompted to investigate, also for conservation purposes, some aspects of its reproductive biology. We aim: (1) to determine if insects play an important role in pollination; (2) to describe the pollinator community; (3) to detect floral scent composition. Experiments of insect exclusion were carried out in the field using 24 flowering individuals: one raceme was capped whereas the nearest one was used as control to ascertain differences in seed set. Pollinator community was detected during the blooming phase of two consecutive flowering seasons by visual observation; insect identification was made at the highest possible taxonomic resolution with the help of digital photographs. In order to determine the chemical composition of the volatiles, we used SPME sampling of cultivated plants. Mann-Whitney U test reveals significant differences for treatment in mean seed set with very low values for capped flowers, thus clearly indicating as insects are crucial for successful pollination. During the 42 h of observations we detected 326 visitors belonging to only three guilds: 79% were Diptera, 20% Hymenoptera and 1% Coleoptera. We identified overall 36 floral organic compounds with only two compounds common to the other studied Berberidaceae. Ambrox was never identified before in the floral scents of any angiosperm. The presence in the scent of several aldehydes and one ketone (benzophenone) could be related to the detected dominance of muscoid flies as pollinators. Floral morphology and composition of the pollinators community indicate a generalist pollination behaviour probably related to its phenology and habitat preference. The possibility of being pollinated also by muscoid flies can be considered an advantage for the reproductive fitness of the species, since these Diptera are abundant in the mountain pastures surrounding the forest habitat of Gymnospermium.

New Alien Plant Taxa for Italy and Europe: An Update.

Despite the wide amount of scientific contributions published on alien plant species, their diffusion dynamics, and their interactions with native taxa, it is increasingly difficult to slow down their spreading and their negative impact on habitats. Last recent years, in fact, a sharp rise in the number of new alien plant taxa introduced in Italy and Europe has been recorded. The aim of this work is to investigate most of the Italian territory in order to verify whether this alarming trend is still underway. Specimen collections and/or observations of alien plants have been performed in as many as 12 Italian regions. All the collected specimens are stored in public or private herbaria. Taxa have been identified according to the literature from the countries of origin of the investigated taxa, while the nomenclature followed the current international references. Updates on 106 taxa are reported. In particular, among 117 new records, 89 are first records, 27 are changes to status and there is 1 extinction. Seven new taxa for Italian alien flora are reported, two of which are new to Europe. The administrative regions with the highest number of records are Calabria (48), Sardegna (17) and Sicilia (15). Five of the surveyed taxa, for the first time, have been considered invasive aliens to Italian territory. The unfrequent amount of original results provided by this work, over the simple importance of data itself, proves how floristic investigation, still today, represents one of the most effective tools in broadening the current knowledge about alien taxa and their dynamics.

Plant invasion risk inside and outside protected areas: Propagule pressure, abiotic and biotic factors definitively matter.

Invasive alien species are among the main global drivers of biodiversity loss posing major challenges to nature conservation and to managers of protected areas. The present study applied a methodological framework that combined invasive Species Distribution Models, based on propagule pressure, abiotic and biotic factors for 14 invasive alien plants of Union concern in Italy, with the local interpretable model-agnostic explanation analysis aiming to map, evaluate and analyse the risk of plant invasions across the country, inside and outside the network of protected areas. Using a hierarchical invasive Species Distribution Model, we explored the combined effect of propagule pressure, abiotic and biotic factors on shaping invasive alien plant occurrence across three biogeographic regions (Alpine, Continental, and Mediterranean) and realms (terrestrial and aquatic) in Italy. We disentangled the role of propagule pressure, abiotic and biotic factors on invasive alien plant distribution and projected invasion risk maps. We compared the risk posed by invasive alien plants inside and outside protected areas. Invasive alien plant distribution varied across biogeographic regions and realms and unevenly threatens protected areas. As an alien's occurrence and risk on a national scale are linked with abiotic factors followed by propagule pressure, their local distribution in protected areas is shaped by propagule pressure and biotic filters. The proposed modelling framework for the assessment of the risk posed by invasive alien plants across spatial scales and under different protection regimes represents an attempt to fill the gap between theory and practice in conservation planning helping to identify scale, site, and species-specific priorities of management, monitoring and control actions. Based on solid theory and on free geographic information, it has great potential for application to wider networks of protected areas in the world and to any invasive alien plant, aiding improved management strategies claimed by the environmental legislation and national and global strategies.

Habitat Fragmentation and Lichen Diversity in Peri-Urban Woodlands: A Case Study in the Municipality of Potenza (Southern Italy).

The fragmentation of the natural habitat is a process that is exponentially increasing worldwide and represents one of the biggest threats to biological diversity. Habitat destruction and fragmentation have a major impact on landscapes and may also affect ecosystems, populations, and species. The ongoing anthropogenic process can result in habitat loss for some species, habitat creation for others, reduced patch size, and increased distance between patches, which may lead to local extinction. We analyzed the effects of patch size and isolation on lichens in Quercus pubescens woods surrounding the city of Potenza (south Italy). We randomly sampled 11 forest patches with homogeneous environmental variables using circular plots with a 10 m radius; the patches ranged from 0.3 to 30 ha. For each plot, we collected data about presence and abundance of epiphytic lichens. We performed the analyses at the patch level using linear regression and multivariate analysis, searching for effects on species richness, life forms, and community compositions. Multivariate analyses were used to study the effect of fragmentation on the structure of lichen vegetation. We investigated the main predictor of lichen species richness in habitat fragmentations and concluded that patch area per se is an important (positive) driver of lichen species richness in Mediterranean peri-urban forests.

Diet Selection by the Italian Hare (Lepus corsicanus de Winton, 1898) in Two Protected Coastal Areas of Latium.

This study was focused on the diet and feeding behaviour of Lepus corsicanus in two protected coastal areas of Latium, Castelporziano Presidential Estate (CPE) and Circeo National Park (CNP). Plant frequency was assessed by the quadrat method, while diet composition was determined by microhistological analysis of faecal samples. Over the year, the Italian hare fed on 185 of the 229 plant species identified in vegetation, with most of them ingested in low percentages (≤1%). During the dry season (DS), in both areas, Brachypodium sylvaticum, Cynodon dactylon, and Avena fatua were among the most consumed species. In the wet season (WS) the most common plant species in diet were B. sylvaticum, Poa trivialis, and Carex distachya in CPE and Dactylis glomerata, Cynosurus echinatus, and Spartium junceum in CNP. In both sites, considering the annual selection of life forms, grasses and leguminous forbs were preferred, while non-leguminous forbs and shrubs were used less than expected according to their availability. ANOSIM analysis showed significant differences between sites in DS and WS diets. Our study evidenced that the Italian hare behaved as generalist, revealing its capability for exploiting several plant species and to adapt its diet preferences to space-time variation of food availability.

Feeding Preferences of the Italian Roe Deer (Capreolus capreolus italicus Festa, 1925) in a Coastal Mediterranean Environment.

The present study was aimed to deepen the knowledge on diet and on feeding preferences of the Italian roe deer. The research was carried on in the Castelporziano Presidential Estate, a protected area of Latium coast. Quadrat method was used to assess plant frequency, whereas diet composition was determined by fecal micro-histological analysis. A total of 143 plant species were identified in the site and 109 of them were found in the feces of the Capreolus capreolus italicus. All over the year, most of the taxa were ingested in low percentages (≤5%) and the most utilized plant species were Quercus suber, Prunus spinosa, Rubia peregrina, and Crateaegus monogyna. Fagaceae and Rosaceae plant families represented the bulk of the diet and were positively selected. In addition, a positive selection on Fabaceae and Oleaceae families was found in wet season but not in dry one. In both periods the Italian roe deer showed a positive selection for deciduous woody plants and evergreen woody plants, instead avoided wild forbs and wild graminoids. Our results confirmed that this subspecies behaved as a generalist highly selective feeder.