Chlorination of microcystin-LR in natural water: Kinetics, transformation products, and genotoxicity.

Microcystin-LR (MC-LR), a type of cyanotoxin commonly found in natural water bodies (sources of drinking water), poses a threat to human health due to its high toxicity. It is essential to successfully remove this cyanotoxin from drinking water sources. In this study, chlorine was used to oxidize MC-LR in Milli-Q water (MQ) (control test) and natural water collected from Lake Longhu (LLW) as a drinking water source. The removal efficiency, proposed transformation pathways, and genotoxicity were investigated. In the chlorine dose range investigated (4.0 mg L-1 - 8.0 mg L-1), the apparent second-order rate constants for MC-LR chlorination varied from 21.3 M-1s-1 to 31.9 M-1s-1 in MQ, higher than that in LLW (9.06 M-1s-1 to 17.7 M-1s-1) due to a faster chlorine decay attributed to the water matrix (e.g., natural organic matter) of LLW. Eleven transformation products (TPs) of MC-LR were identified in the two waters. The conjugated diene moieties and benzene ring of Adda moiety (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid), and the double bond of Mdha moiety (N-methyldehydroalanine) were the major susceptible reaction sites. Attacking unsaturated bonds by hydroxyl and chlorine radicals to generate monochloro-hydroxy-MC-LR was the primary initial transformation pathway, followed by nucleophilic substitution, dehydration, and cleavage in MC-LR. Chlorine substitution on the benzene ring was also observed. Based on the bacterial reverse-mutation assay (Ames assay), TPs in treated natural water did not induce genotoxicity/mutagenicity. These findings shed light on the role of chlorination in controlling the risk of cyanotoxins in drinking water treatment plants.

The insufficient extraction of DNA from swine manures may underestimate the abundance of antibiotic resistance genes as well as ignore their potential hosts.

Swine manure is considered as an extensive reservoir of antibiotic resistance genes (ARGs) spreading in the natural environment after application in soils. To understand whether ARGs abundance in swine manure is underestimated and the hosts are ignored, this study successively extracted DNA from swine manure six times and determined the abundance of several ARGs, class I integron (intI1), and 16S rRNA as well as the microbial communities. It is found that successive six DNA extraction of swine manures elevated the yield of DNA and strongly increased the abundance of ARGs, intI1, and 16S rRNA. Compared with single DNA extraction, the most dominant bacterial phylum in swine manures shifted from Proteobacteria to Firmicutes, and the dominant bacterial genera changed from Acinetobacter Clostridium after six DNA extraction. The ignored abundance of bacterial phylum and genus emphasized the possible hosts carrying these genes should be paid more attention. It is suggested that the successive DNA extraction of manures is required in the future study to improve the knowledge of estimating the risk and hosts of ARGs in manures entering the environment.

Contrasting effects of phytoplankton aging on microplastic antibiotic adsorption depending on species tolerance, and biofouling level.

Recent studies have reported conflicting results on the effects of biofouling on the adsorption behavior of microplastics (MPs). However, the underlying mechanisms driving the adsorption of MPs undergoing biofouling in aquatic environments remain unclear. This study examined the interactions between polyamide (PA), polyvinyl chloride (PVC) and polyethylene (PE) with two phytoplankton, namely cyanobacteria Microcystis aeruginosa and microalgae Chlorella vulgaris. Results indicated that MP effects on phytoplankton were dose- and crystalline-type dependent, with M. aeruginosa being more sensitive to MP exposure than C. vulgaris in the inhibitory order PA > PE > PVC. Analysis of antibiotic adsorption of the MPs showed significant contributions from CH/π interactions on PE and PVC and hydrogen bonding on PA, which decreased with phytoplankton biofouling and aging. Meanwhile, higher levels of extracellular polymeric substances on microalgae-aged MPs compared to cyanobacteria-aged MPs were conducive to adsorption of antibiotics, mainly through hydrophobic interactions. Overall, promotional and anti-promotional adsorption of antibiotics on MPs was induced by biofouling and aging of microalgae and cyanobacteria, respectively. This study provides deep insights into the specific mechanisms by which biofouling affects MP adsorption in aquatic environments, thus advancing our understanding of this critical environmental issue.

The relationship between dietary vitamin A intake and the levels of sialic acid in the breast milk of lactating women.

Sialic acid (SA) is an important nutrient but few studies have examined the link between dietary intake and breast milk sialic acid. The purpose of this observational study was to assess the potential relationship between human breast milk sialic acid levels and dietary nutrition intake 40 d postpartum. The study population included 90 healthy women who were lactating. Human breast milk SA concentrations were measured using fluorescence detector-high performance liquid chromatography (HPLC-FLD) analysis and nutritional intake was estimated by a computerized validated food frequency questionnaire. SA in human breast milk was bound to free oligosaccharides (82.35%), protein (15.27%) and free sialic acid (2.37%). The findings of this study indicate that subjects with higher milk SA levels showed statistically higher levels of vitamin A compared with subjects with lower SA levels (423.48±172.29 vs. 602.22±126.46 µg/d, p=0.000). In addition, there was a certain association (standardized coefficients=0.713; p=0.000) between breast milk SA and vitamin A intake in healthy young subjects. This study demonstrated that dietary vitamin A intake has a certain relationship with breast milk SA concentrations. This may be attributed to the influence of vitamin A on sialic acid glycoprotein and sialic acid mucopolysaccharide in the human body or the common food sources for vitamin A and sialic acid. Additional study is required to further investigate this relationship.