Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection.

OBJECTIVE: An increase in the number of neutrophils (NEUs) has long been associated with infections in the knee joints; however, their impact on knee osteoarthritis (KOA) pathophysiology remains largely unexplored. DESIGN: This study compared the phenotypic and functional characteristics of synovial fluid (SF)-derived NEUs in KOA and knee infection (INF). RESULTS: KOA NEUs were characterised by a lower expression of CD11b, CD54, and CD64 and higher expression of CD62L, TLR2, and TLR4 compared with INF NEUs. Except for CCL2, lower levels of inflammatory mediators and proteases were detected in KOA SF than in INF SF. Functionally, KOA NEUs displayed increased reactive oxygen species production and phagocytic activity compared with INF NEUs. Moreover, KOA and INF NEUs differed in cell sizes, histological characteristics of the surrounding synovial tissues, and their effects on the endothelial cells assessed by human umbilical vein endothelial cells. When KOA patients were subdivided based on the SF NEU abundance, patients with high NEUs (10%-60%) were characterised by i) elevated SF protein levels of TNF-α, IL-1RA, MMP-9, sTREM-1, VILIP-1 and ii) lower CD54, CD64, TLR2 and TLR4 expression compared to patients with low NEUs (<10%). Analysis of paired SF samples suggests that low or high NEU percentages, respectively, persist throughout the course of disease. CONCLUSIONS: Our findings suggest that NEU may play a significant role in KOA pathophysiology. Further studies should explore the mechanisms that contribute to the increased number of NEUs in SF and the clinical consequences of neutrophilic phenotype in KOA.

[Effect of a Single Dose of Tranexamic Acid Administered during Anterior Cruciate Ligament Reconstruction Using Hamstrings: a Randomized Clinical Study]. / Efekt jednorázového podání kyseliny tranexamové pri rekonstrukci predního zkrízeného vazu hamstringy: randomizovaná klinická studie.

PURPOSE OF THE STUDY Anterior cruciate ligament reconstruction is one of the most common reconstruction surgeries. The unintended consequences of the surgery are hemarthrosis, blood loss, knee swelling and postoperative pain. The purpose of the study was to evaluate the effect of a single dose of intravenous tranexamic acid (TXA) on the postoperative parameters and functional status of the knee joint 3 months after surgery. MATERIAL AND METHODS It is a prospective randomised clinical study. An intravenous injection of TXA equivalent to 15 mg/kg in 100 ml of saline solution was administered to the test group during the surgery (20 minutes before the end of the surgery). The control group was administered 100 ml of saline solution without TXA. In both groups, the following parameters were evaluated preoperatively and postoperatively (on Day 1 and Day 10 and at 1 month and 3 months): thigh circumference at 1 cm above the patella, Coupens and Yates (CY) score for swelling, and pain score (VAS). At 24 hours after the surgery, the blood loss (secretion into the drain) and decrease in hemoglobin (Hb) and hematocrit (HCT) levels compared to the preoperative levels were assessed. The functional status of the knee joint was assessed based on the Lysholm knee scoring scale and the IKDC subjective knee evaluation form preoperatively, or at 1 and 3 months postoperatively. RESULTS In the test group, a significantly lower blood loss was detected 24 hours after the surgery. The mean difference of 128 ml compared to the control group was both statistically and practically significant (p < 0.001, d =1.42). The test group showed a lower decrease in Hb and HCT levels postoperatively compared to the control group, although with no statistical significance. On the first postoperative day, slightly better results of the thigh circumference at 1 cm above the patella and of the CY score were observed in the test group. However, during the follow-up check performed postoperatively on Day 10, the differences in the thigh circumference at 1 cm above the patella, CY score and pain VAS score were negligible. The differences in the functional status of the knee joint between the two groups ascertained during the check performed 1 month and 3 months after the surgery were insignificant. DISCUSSION Our study, just like other studies, confirms a significant effect of a single dose of intravenous TXA on the volume of blood loss and early postoperative swelling, which are the parameters affecting the early postoperative course. Even though the intervention does not affect the subsequent result of surgery, it can undoubtedly be of benefit perioperatively. There is a fairly limited number of randomised clinical studies on this topic in literature, with most of them published in the last 7 years. Further research should, among other things, optimise the protocol and identify a suitable candidate for TXA administration in patients undergoing an ACL reconstruction. CONCLUSIONS Our study confirmed the positive effect of a single dose of intravenous TXA during the reconstruction of anterior cruciate ligament using hamstrings on early postoperative blood loss and early postoperative swelling, which can have a positive effect on wound healing and prevent postoperative complications. Therefore, in agreement with available literature, we recom mend administering a single dose of intravenous TXA in ACL reconstruction, unless there is a contraindication to this therapy. Key words: anterior cruciate ligament reconstruction, hamstrings tranexamic acid, single intravenous administration, clinical evaluation.

[InternalBrace ACL Repair - First Experiences and Outcomes]. / První výsledky a klinické zkusenosti s metodou InternalBrace u lézí LCA.

PURPOSE OF THE STUDY In recent years attempts have been made again to repair anterior cruciate ligament (ACL) in order to maintain native kinematics and knee joint proprioception. The method of choice is the arthroscopic suture of the ligament using the InternalBrace ligament augmentation. This study presents the first experience gained with this surgical technique and the clinical outcomes. MATERIAL AND METHODS In the period from November 2016 to December 2018, a total of 46 patients with acute ACL rupture underwent the ACL repair using the InternalBrace ligament augmentation. This study includes only the patients followed up for a minimum period of 12 months postoperatively. For the evaluation of patients, the well-established clinical scores were used - the Tegner Activity Level Scale, the IKDC (International Knee Documentation Committee) subjective scale and the Tegner Lysholm Knee Scoring Scale. The measurements were taken preoperatively and at 6 and 12 months postoperatively. RESULTS The evaluations concerned 20 patients who had undergone surgery in the period from February 2017 to February 2018. In the aforementioned group of patients, there were 3 cases of reconstruction failure (15%), subsequently requiring the ACL reconstruction. The average preoperative Tegner Activity score was 8.2 (6-10; median 8.0). At six months postoperatively, the average value of this score was 7.35 (5-10; median 7), and at 12 months postoperatively it was 7.35 (4-10; median 7). The average preoperative Lysholm score was 66.4 (16-100; median 69). At six months postoperatively, the average value of this score improved to 90.70 (71-100; median 92) and at 12 months postoperatively to 91 (75-100; median 90). The average IKDC subjective score before the surgery was 49.8 (36-74; median 49). At six months postoperatively the average achieved score was 87.70 (71-99; median 90) and at 12 months postoperatively it was 88.9 (63-99; median 93). The differences between the preoperative values and the values at 6 months after the surgery were significant in all the followedup parameters. Conversely, the differences between the values at 6 and at 12 months were not significant. DISCUSSION The clinical outcomes of the patients after the ACL repair are valuable and comparable to the data published in literature. They were largely achieved within 6 months after the surgery. A fairly high percentage of failure of this method compared to literature (Jonkergouw 7.5%, McKay 1.5%) can partly be ascribed to the learning curve even though the surgeries were performed exclusively by experienced surgeons. A certain role could also be played by more challenging indication for this procedure or a faster return to postoperative load. CONCLUSIONS Anterior cruciate ligament suture with the InternalBrace ligament augmentation judging from the results achieved by our group of patients brings very good clinical outcomes already at 6 months postoperatively. This method allows the athletes to return to full load soon. It maintains the kinematics and knee joint proprioception, and thus can potentially contribute to the prevention of post-traumatic arthritis. Strict application of indication criteria is a precondition to success. A longer follow up and evaluation of a larger number of patients will, however, be necessary to definitely confirm the success of this surgical technique. Key words: arthroscopy, ACL rupture, ACL repair, InternalBrace, clinical evaluation.

Reproducibility of jumping mechanography and traditional measures of physical and muscle function in older adults.

SUMMARY: Improved approaches to assess functional change over time are needed to optimally reduce fall/fracture risk; jumping mechanography (JM) may be one such methodology. In this study, JM parameters were more reproducible than traditional functional tests. JM may be better able to demonstrate efficacy of interventions to mitigate sarcopenia. INTRODUCTION: Jumping mechanography (JM), a tool using maximal countermovement jumps performed on a force plate, may more reliably assess muscle function than traditional methods. The purpose of this study was to examine JM retest reliability in older adults compared with commonly used muscle and physical function assessments. METHODS: Community-dwelling individuals age≥70 years performed physical and muscle function assessments including the short physical performance battery (SPPB), grip strength, and JM on multiple occasions over 3 months. JM parameters included body weight-corrected peak power and jump height. Appendicular lean mass was measured by dual energy x-ray (DXA). Mixed effects linear regression models were used to estimate between- and within-person variability summarized as intra-class correlation coefficients (ICC). RESULTS: Ninety-seven individuals (49 females, 48 males, mean age 80.7 years) participated. All testing was well tolerated; no participant sustained injury. Jump power, height, and grip strength were greater (p<0.0001) in men than women. Grip strength, jump power, and height had excellent ICCs (0.95, 0.93, and 0.88, respectively); chair rise, SPPB score, and gait speed had lower ICCs (0.81, 0.77, and 0.76, respectively). CONCLUSION: In older adults, JM has excellent retest reliability, is stable over time, and can be performed safely. JM retest reliability was comparable to grip strength and possibly better than SPPB and gait speed. JM is a promising tool for muscle function assessment in older adults. Comparison of this approach with traditional assessment tools in longitudinal interventional studies is needed.

[Food intake patterns in a mentally disabled children's community]. / Aspecte ale aportului alimentar intr-o colectivitate de copii cu handicap mental.

AIM: To asses the food intake in a closed community of mentally disabled school children. MATERIAL AND METHOD: The dietary survey was performed according to the ongoing romanian regulations, taking into account 11 food groups. RESULTS: Each of the four dietary surveys showed that certain food groups are present in the diet in very small quantities (such as fish, milk, animal fat, eggs, fruits) and other are present in excess (potatoes, cereals, oils, dry vegetables), not regarding the season of the dietary survey. Even if the energy requirements are met, there is no interest in providing the children with a balanced diet. CONCLUSION: Changes are needed in order to improve the food intake patterns and a more carefully menu planning is required.