Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
Eur Rev Med Pharmacol Sci ; 19(8): 1373-80, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25967711

RESUMO

OBJECTIVE: The acute lymphocytic leukemia is a hematopoietic cancer that occurs predominantly in children. Methotrexate is one of the most useful drugs in cancer chemotherapy. The aim of the study was to develop and validate the methodology of high performance liquid chromatography (HPLC) with ultraviolet detection for methotrexate dosage and to determine its concentration in plasma samples from children with leukemia. PATIENTS AND METHODS: The study included patients from the outpatient care of pediatric oncology at the Faculty of Medicine of ABC carriers in treatment of leukemia. The study was conducted in chromatographic model Agilent 1100 with UV detector at 302 nm and by the method of ELISA microplate reader capable of reading absorbance at 450 nm. RESULTS: We obtained satisfactory results of selectivity, accuracy, linearity, limit of quantification (LOQ), limit of detection (LOD), precision and robustness and apply the basic criteria for validation as RE No. 899, of May 29, 2003 Guide validation of analytical and bioanalytical National Agency Health Surveillance (ANVISA). CONCLUSIONS: We conclude that results for linearity/concentration range, precision, robustness, limit of quantification and detection limits are within the acceptance criteria defined by ANVISA and that the developed analytical method is valid and feasible to be used as a tool in monitoring therapy of methotrexate.


Assuntos
Antimetabólitos Antineoplásicos/sangue , Cromatografia Líquida de Alta Pressão/normas , Leucemia/sangue , Metotrexato/sangue , Antimetabólitos Antineoplásicos/uso terapêutico , Criança , Cromatografia Líquida de Alta Pressão/tendências , Humanos , Leucemia/tratamento farmacológico , Limite de Detecção , Metotrexato/uso terapêutico , Reprodutibilidade dos Testes
2.
Eur Rev Med Pharmacol Sci ; 19(17): 3229-33, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26400527

RESUMO

OBJECTIVE: The mismatch repair (MMR) genes play a central role for the onset of cancer. One of these genes is hMSH2. A differential hMSH2 protein expression has been detected in the mononuclear fraction of peripheral blood of patients with breast cancer when compared to healthy women. This work aims to evaluate the expression of hMSH2 in patients diagnosed with breast cancer undergoing treatment at various stages of the disease to verify its potential use as a prognostic marker. PATIENTS AND METHODS: Immunohistochemical expression of hMSH2 at different stages of breast cancer in 40 patients biopsy samples were analyzed. RESULTS: hMSH2 has a considerable increased expression in all groups of patients with tumors, when compared to patients without tumors. CONCLUSIONS: immunohistochemistry indeed can be a great tool for the diagnosis of breast cancer, as it is an easy and versatile technique.


Assuntos
Neoplasias da Mama/genética , Instabilidade Genômica/genética , Linfonodos/metabolismo , Proteína 2 Homóloga a MutS/metabolismo , Proteínas Nucleares/genética , Proteínas Adaptadoras de Transdução de Sinal/genética , Adulto , Neoplasias da Mama/patologia , Reparo de Erro de Pareamento de DNA , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA