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1.
Allergol Immunopathol (Madr) ; 43(3): 272-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25796303

RESUMO

BACKGROUND: PIDs are a heterogeneous group of genetic illnesses, and delay in their diagnosis is thought to be caused by a lack of awareness among physicians concerning PIDs. The latter is what we aimed to evaluate in Brazil. METHODS: Physicians working at general hospitals all over the country were asked to complete a 14-item questionnaire. One of the questions described 25 clinical situations that could be associated with PIDs and a score was created based on percentages of appropriate answers. RESULTS: A total of 4026 physicians participated in the study: 1628 paediatricians (40.4%), 1436 clinicians (35.7%), and 962 surgeons (23.9%). About 67% of the physicians had learned about PIDs in medical school or residency training, 84.6% evaluated patients who frequently took antibiotics, but only 40.3% of them participated in the immunological evaluation of these patients. Seventy-seven percent of the participating physicians were not familiar with the warning signs for PIDs. The mean score of correct answers for the 25 clinical situations was 48.08% (±16.06). Only 18.3% of the paediatricians, 7.4% of the clinicians, and 5.8% of the surgeons answered at least 2/3 of these situations appropriately. CONCLUSIONS: There is a lack of medical awareness concerning PIDs, even among paediatricians, who have been targeted with PID educational programmes in recent years in Brazil. An increase in awareness with regard to these disorders within the medical community is an important step towards improving recognition and treatment of PIDs.


Assuntos
Competência Clínica/estatística & dados numéricos , Síndromes de Imunodeficiência/epidemiologia , Médicos/estatística & dados numéricos , Brasil , Estudos Transversais , Cirurgia Geral , Hospitais Gerais , Humanos , Síndromes de Imunodeficiência/diagnóstico , Medicina Interna , Pediatria , Papel do Médico , Prática Profissional , Inquéritos e Questionários
2.
Infect Dis Now ; 53(4): 104708, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37054972

RESUMO

OBJECTIVES: Dengue fever is an arbovirosis expanding worldwide, for which hydration has been reported to reduce the risk of hospitalization. Our objective was to estimate the volume of hydration in Reunionese patients with dengue. METHODS: A prospective observational study included patients presenting with a 'dengue-like' syndrome in ambulatory care. General practitioners recruited patients during consultation, and beverage consumption over the previous 24 hours was reported at two different times. Warning signs were defined according to the 2009 WHO guidelines. RESULTS: GPs included 174 patients from April to July 2019. Average oral hydration volume was 1863 mL and 1944 mL, at the 1st and 2nd medical consultations, respectively. Water was the most wide consumed liquid. Drinking at least 5 glasses of liquid was significantly associated with fewer clinical warning signs at the 1st medical consultation (p = 0.044). CONCLUSIONS: Sufficient hydration volume could prevent dengue warning signs. Further studies with standardized measurement of hydration would be needed.


Assuntos
Dengue , Humanos , Dengue/diagnóstico , Dengue/prevenção & controle , Estudos Prospectivos , Hospitalização , Assistência Ambulatorial , Atenção Primária à Saúde
3.
ACS Appl Polym Mater ; 4(10): 7217-7227, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-37808584

RESUMO

The development of environment-friendly natural polymer gel-like dispersions in oil media, with functional properties, in terms of formulation design and synthesis protocol, is still a cutting-edge research area for many applications. The aim of this work was to study the manufacture of electrospun ethylcellulose (EC) nanofibrous webs and to examine their usage to thicken vegetable oils as an alternative approach. The role of concentration, molecular weight (Mw), and binary solvent systems on the electrospinnability of EC and subsequently on the rheological properties of EC nanofiber dispersions in castor oil was investigated. It was observed that, for each Mw, defect-free nanofibers were produced above a critical concentration, corresponding to about 2.5 the entanglement concentration (Ce). The average fiber diameter increased with both Mw and EC concentrations. Dielectric constant and dipole moment of binary solvent systems influenced the morphology of the EC nanofiber web. The morphology of the micro- and nanoarchitectures generated played a key role in the physical stabilization and rheological behavior of electrospun EC dispersions. The storage modulus (G') of EC dispersions was correlated with both the spinning solution concentration and average fiber diameter. Furthermore, electrospun EC nanofiber dispersions were compared with EC oleogels obtained by traditional thermogelation from thermorheological and tribological points of view. Overall, this work proposes an efficient and innovative approach to produce bio-based oleogel-like dispersions with great potential in different sectors such as pharmaceuticals, food, or lubricants.

4.
Med Trop Sante Int ; 1(1)2021 03 31.
Artigo em Francês | MEDLINE | ID: mdl-35586641

RESUMO

Introduction: Chlamydia trachomatis (CT) infection is the commonest bacterial sexually transmitted infection (STI) in the world. Often asymptomatic, it can lead to significant complications in women. In France, since 2003, systematic screening for CT in STI center has been recommended for women aged less than 25 year. The main objective of this study was to determine CT prevalence in patients attending STI centers in Reunion Island. The second objective was to explore the determinants of this infection. Method: A cross-sectional survey using an anonymous questionnaire was conducted among women attending STI center in two hospitals in western and southern Reunion Island during one year. All women who had performed a CT PCR, based on vaginal self-swabs, were included. Results: Among the 620 patients tested, the prevalence of infection was 6.6% (95% CI [4.7-8.6]). By age group, the highest prevalence was between 12 and 17 years with 14.3% positive tests compared to 7.5% and 3.9% respectively in 18-24 and 25-67 year age group (p = 0.003). The risk factors for CT were a young age (p = 0.02), a first sexual intercourse between 11 and 14 years old (p = 0.01), lack of previous STI screening history (p = 0.02), and the following motives for seeking screening: "partner unfaithfulness" (p = 0.01) and "infected partner" (p = 0.02). Conclusion: This study highlights the high prevalence of CT among Reunionese minors. A more systematic screening and a reinforcement of STI awareness among young people in Reunion Island seem to be essential.


Assuntos
Infecções por Chlamydia , Infecções Sexualmente Transmissíveis , Adolescente , Criança , Pré-Escolar , Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis , Estudos Transversais , Feminino , Humanos , Prevalência , Reunião/epidemiologia , Fatores de Risco , Infecções Sexualmente Transmissíveis/epidemiologia
6.
Schizophr Res ; 207: 80-92, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-29730045

RESUMO

Mounting evidence indicates that schizophrenia is a disorder that stems from maladaptive plasticity within neural circuits and produces broad cognitive deficits leading to loss of autonomy. A large number of studies have identified abnormalities spanning many neurotransmitter systems in schizophrenia, and as a result, a variety of drugs have been developed to attempt to treat these abnormalities and enhance cognition. Unfortunately, positive results have been limited so far. This may be in part because the scope of abnormalities in the schizophrenic brain requires a treatment capable of engaging many different neurotransmitter systems. One approach to achieving this kind of treatment has been to use neuroplasticity-based computerized cognitive training programs to stimulate the formation of more adaptive circuits. Although the number of studies implementing this approach has increased exponentially in recent years, effect sizes for cognitive gains have been modest and adherence to treatment remains an important challenge in many studies, as patients are often required to train for 40 h or more. In the present paper, we argue that cognitive training protocols will benefit from the addition of cognitive enhancers to produce more robust and longer lasting targeted neuroplasticity. Indeed, recent data from animal studies have provided support for combining plasticity-enhancing drugs with tailored behavioral training paradigms to restore normal function within dysfunctioning neural circuits. The advantages and challenges of applying this approach to patients with schizophrenia will be discussed.


Assuntos
Disfunção Cognitiva , Remediação Cognitiva , Plasticidade Neuronal , Neurotransmissores/uso terapêutico , Esquizofrenia , Disfunção Cognitiva/tratamento farmacológico , Disfunção Cognitiva/etiologia , Disfunção Cognitiva/metabolismo , Disfunção Cognitiva/reabilitação , Terapia Combinada , Humanos , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Esquizofrenia/complicações , Esquizofrenia/tratamento farmacológico , Esquizofrenia/metabolismo , Esquizofrenia/reabilitação
7.
Food Chem Toxicol ; 46(8): 2639-47, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18534735

RESUMO

Nowadays, a variety of protocols are applied to quantitate palytoxin. However, there is not desirable agreement among them, the confidence intervals of the basic toxicological parameters are too wide and the formal descriptions lack the necessary generality to establish comparisons. Currently, the mouse bioassay is the most accepted one to categorize marine toxins and it must constitute the reference for other methods. In the present work, the mouse bioassay for palytoxin is deeply analyzed and carefully described showing the initial symptoms of injected mice which are presented here in the first time. These symptoms clearly differ from the more common marine toxins described up to now. Regarding to the toxicological aspects two considerations are taking into account: (i) the empiric models based in the dose-death time relationships cause serious ambiguities and (ii) the traditional moving average method contains in its regular use any inaccuracy elements. Herein is demonstrated that the logistic equation and the accumulative function of Weibull's distribution (with the modifications proposed) generate satisfactory toxicological descriptions in all the respects.


Assuntos
Acrilamidas/toxicidade , Algoritmos , Animais , Bioensaio , Venenos de Cnidários , Interpretação Estatística de Dados , Relação Dose-Resposta a Droga , Injeções Intraperitoneais , Modelos Logísticos , Masculino , Camundongos , Modelos Estatísticos , Análise de Sobrevida , Fatores de Tempo
8.
Carbohydr Polym ; 199: 563-571, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30143163

RESUMO

Several di- or tri-functional epoxides were used to chemically modify an industrial grade cellulose pulp from Eucalyptus globulus in order to thicken castor oil by dispersing epoxidized cellulose pulp fibers, resulting gel-like formulations with potential applications as biolubricants. Rheological properties of these colloidal suspensions were evaluated by analysing the effects of epoxidation degree and the epoxide chemical structure. With this aim, epoxidized cellulose pulp samples were characterized by means of epoxy index determination, thermogravimetric analysis (TGA) and Fourier transform infrared (FTIR) spectroscopy. It was found that linear viscoelastic functions, determined in small-amplitude oscillatory shear (SAOS), and viscosity values of epoxidized cellulose pulp gel-like dispersions decreased by increasing the epoxy index, whereas the shear-thinning character was dampened with the extension of epoxidation. Aromatic di-functional epoxides provide higher values of these rheological functions than aliphatic ones.

9.
Theriogenology ; 66(2): 224-33, 2006 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-16325250

RESUMO

Pregnancy rates following transfer of an in vitro-produced (IVP) embryo are often lower than those obtained following transfer of an embryo produced by superovulation. The purpose of the current pair of experiments was to examine two strategies for increasing pregnancy rates in heat stressed, dairy recipients receiving an IVP embryo. One method was to transfer two embryos into the uterine horn ipsilateral to the CL, whereas the other method involved injection of GnRH at Day 11 after the anticipated day of ovulation. In Experiment 1, 32 virgin crossbred heifers and 26 lactating crossbred cows were prepared for timed embryo transfer by being subjected to a timed ovulation protocol. Those having a palpable CL were randomly selected to receive one (n = 31 recipients) or two (n = 27 recipients) embryos on Day 7 after anticipated ovulation. At Day 64 of gestation, the pregnancy rate tended to be higher (P = 0.07) for cows than for heifers. Heifers that received one embryo tended to have a higher pregnancy rate than those that received two embryos (41% versus 20%, respectively) while there was no difference in pregnancy rate for cows that received one or two embryos (57% versus 50%, respectively). Pregnancy loss between Day 64 and 127 only occurred for cows that received two embryos (pregnancy rate at Day 127=17%). Between Day 127 and term, one animal (a cow with a single embryo) lost its pregnancy. There was no difference in pregnancy rates at Day 127 or calving rates between cows and heifers, but females that received two embryos had lower Day-127 pregnancy rates and calving rates than females that received one embryo (P < 0.03). Of the females receiving two embryos that calved, 2 of 5 gave birth to twins. For Experiment 2, 87 multiparous, late lactation, nonpregnant Holstein cows were synchronized for timed embryo transfer as in Experiment 1. Cows received a single embryo in the uterine horn ipsilateral to the ovary containing the CL and received either 100 microg GnRH or vehicle at Day 11 after anticipated ovulation (i.e. 4 days after embryo transfer). There was no difference in pregnancy rate for cows that received the GnRH or vehicle treatment (18% versus 17%, respectively). In conclusion, neither unilateral transfer of two embryos nor administration of GnRH at Day 11 after anticipated ovulation improved pregnancy rates of dairy cattle exposed to heat stress.


Assuntos
Bovinos/fisiologia , Transferência Embrionária/veterinária , Fármacos para a Fertilidade Feminina/administração & dosagem , Hormônio Liberador de Gonadotropina/administração & dosagem , Temperatura Alta , Taxa de Gravidez , Animais , Bovinos/embriologia , Feminino , Fertilização in vitro/veterinária , Inseminação Artificial/veterinária , Paridade , Gravidez , Distribuição Aleatória , Fatores de Tempo
10.
Phys Rev E ; 94(6-1): 062402, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28085424

RESUMO

Growing living cultures of Escherichia coli bacteria are investigated using real-time in situ rheology and rheoimaging measurements. In the early stages of growth (lag phase) and when subjected to a constant stationary shear, the viscosity slowly increases with the cell's population. As the bacteria reach the exponential phase of growth, the viscosity increases rapidly, with sudden and temporary abrupt decreases and recoveries. At a certain stage, corresponding grossly to the late phase of growth, when the population stabilizes, the viscosity also keeps its maximum constant value, with drops and recoveries, for a long period of time. This complex rheological behavior, which is observed to be shear strain dependent, is a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties. Particular attention is given to the late phase of growth of E. coli populations under shear. Rheoimaging measurements reveal, near the static plate, a rotational motion of E. coli aggregates, collectively tumbling and flowing in the shear direction. This behavior is interpreted in the light of a simple theoretical approach based on simple rigid body mechanics.


Assuntos
Escherichia coli/fisiologia , Modelos Biológicos , Escherichia coli/crescimento & desenvolvimento , Movimento (Física) , Viscosidade
11.
Nanoscale ; 7(45): 19259-72, 2015 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-26531801

RESUMO

To take full advantage of the remarkable applications of carbon nanotubes in different fields, there is a need to develop effective methods to improve their water dispersion and biocompatibility while maintaining their physical properties. In this sense, current approaches suffer from serious drawbacks such as loss of electronic structure together with low surface coverage in the case of covalent functionalizations, or instability of the dynamic hybrids obtained by non-covalent functionalizations. In the present work, we examined the molecular basis of an original strategy that combines the advantages of both functionalizations without their main drawbacks. The hierarchical self-assembly of diacetylenic-based neoglycolipids into highly organized and compacted rings around the nanotubes, followed by photopolymerization leads to the formation of nanotubes covered with glyconanorings with a shish kebab-type topology exposing the carbohydrate ligands to the water phase in a multivalent fashion. The glyconanotubes obtained are fully functional, and able to establish specific interactions with their cognate receptors. In fact, by taking advantage of this selective binding, an easy method to sense lectins as a working model of toxin detection was developed based on a simple analysis of TEM images. Remarkably, different experimental settings to assess cell membrane integrity, cell growth kinetics and cell cycle demonstrated the cellular biocompatibility of the sugar-coated carbon nanotubes compared to pristine single-walled carbon nanotubes.


Assuntos
Carboidratos , Lectinas/metabolismo , Nanotubos de Carbono/química , Animais , Carboidratos/química , Carboidratos/farmacologia , Linhagem Celular , Camundongos
12.
AIDS ; 14(6): 727-32, 2000 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-10807196

RESUMO

OBJECTIVE: To examine the prevalence of resistance mutations to nucleoside reverse transcriptase inhibitors (NRTI) and protease inhibitors (PI) in a representative HIV-1 population in Spain. METHODS: A cross-sectional study was conducted including 601 HIV-infected patients who attended 20 Spanish hospitals in June 1998. Drug resistant mutations were examined using hybridization line probe assays (LiPA). The 6 bp insert at position 69 and the codon 75 mutant were examined by sequencing analysis in specimens lacking reactivity to 69/70 and 74 bands on LiPA, respectively. RESULTS: Primary resistance to NRTI was recognized in nine out of 52 (17%) naive individuals, whereas primary resistance to PI was found in seven out of 126 (6%) PI-naïve patients. The codons most frequently involved in NRTI resistance were at positions 70 (66%), 184 (44%), 215 (33%), and 41 (11%), whereas the most common PI resistance mutation was at codon 82 (6/7 subjects). In pre-treated patients, the overall prevalence of resistant genotypes was 72.9% for NRTI and 27.2% for PI. The most frequent NRTI mutations occurred at codons 184 (38.5%), 215 (30.1%), and 41 (22.5%), whereas the most frequent PI mutations in pre-treated subjects were found at positions 82 (15.8%) and 84 (11.4%). Overall, patients who began triple combinations as initial therapy showed a lower number of key resistance mutations than those who began highly active antiretroviral therapy (HAART) after being exposed to NRTI for a period of time (mean number of mutations, 0.1 versus 1.8, P< 0.05). Codon 75 mutant was found in three out of 387 patients (0.7%), whereas no insertions at codon 69 were recognized. CONCLUSION: The prevalence of primary genotypic resistance to NRTI and PI in Spain was 17% and 6%, respectively. Zidovudine, lamivudine, indinavir and ritonavir were the drugs most frequently affected. These data support the use of resistance testing prior to the introduction of first-line antiretroviral therapies in Spain. Among pre-treated subjects, drug resistance genotypes were less prevalent in those who began HAART as initial therapy.


Assuntos
Fármacos Anti-HIV/farmacologia , Infecções por HIV/epidemiologia , Infecções por HIV/virologia , Inibidores da Protease de HIV/farmacologia , HIV-1/efeitos dos fármacos , Inibidores da Transcriptase Reversa/farmacologia , Adulto , Fármacos Anti-HIV/uso terapêutico , Estudos Transversais , Resistência Microbiana a Medicamentos/genética , Feminino , Genótipo , Protease de HIV/genética , Inibidores da Protease de HIV/uso terapêutico , Transcriptase Reversa do HIV/genética , HIV-1/genética , Humanos , Masculino , Mutação , Prevalência , Inibidores da Transcriptase Reversa/uso terapêutico , Espanha/epidemiologia
13.
Aliment Pharmacol Ther ; 13(12): 1647-52, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10594400

RESUMO

AIM: To evaluate the efficacy of omeprazole plus clarithromycin and furazolidone in Helicobacter pylori eradication and duodenal ulcer healing in Brazilian patients. METHODS: Forty H. pylori-positive patients with duodenal ulcer were randomized to receive 20 mg omeprazole o.m. or b.d. for 1 month plus 500 mg clarithromycin (b.d. ) and 200 mg furazolidone (b.d.) for 1 week. RESULTS: Three months after the end of the treatment the eradication rates were 90% by intention-to-treat analysis, and 97% by per protocol analysis. Mild side-effects were observed in 25 patients, none of whom abandoned the protocol. No difference was observed between the 20 mg and 40 mg omeprazole daily doses. Cure or significant improvement of the symptoms and of the histological alterations were observed after H. pylori eradication. CONCLUSION: Our results demonstrate that clarithromycin and furazolidone in combination with omeprazole are a good alternative for H. pylori eradication in Brazilian patients with duodenal ulcer.


Assuntos
Claritromicina/administração & dosagem , Úlcera Duodenal/tratamento farmacológico , Furazolidona/administração & dosagem , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori , Omeprazol/administração & dosagem , Adolescente , Adulto , Antibacterianos/administração & dosagem , Antibacterianos/efeitos adversos , Antiulcerosos/administração & dosagem , Antiulcerosos/efeitos adversos , Brasil , Claritromicina/efeitos adversos , Quimioterapia Combinada , Úlcera Duodenal/patologia , Endoscopia , Feminino , Furazolidona/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Omeprazol/efeitos adversos , Distribuição Aleatória , Fatores de Tempo
14.
AJNR Am J Neuroradiol ; 4(3): 816-8, 1983.
Artigo em Inglês | MEDLINE | ID: mdl-6410861

RESUMO

Three cases of chiasmal optic neuritis are described with bitemporal visual field loss and enlargement of the optic chiasm as demonstrated by computed tomography (CT). Exploratory craniotomies were performed in two of the patients, but no tumor was found. After corticosteroid treatment, all three patients experienced virtually complete restoration of normal vision. Although the CT presentation in these patients was indistinguishable from that of a chiasmal glioma, the clinical presentation of acute visual loss in an older child or adult should suggest chiasmal optic neuritis. A period of observation, with or without a trial of corticosteroids, might permit the latter diagnosis and obviate exploratory craniotomy.


Assuntos
Quiasma Óptico/diagnóstico por imagem , Neurite Óptica/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adolescente , Adulto , Criança , Diagnóstico Diferencial , Feminino , Humanos , Masculino
15.
Braz J Med Biol Res ; 32(12): 1493-7, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10585630

RESUMO

The aim of this work was to compare the performance of isotope-selective non-dispersive infrared spectrometry (IRIS) for the 13C-urea breath test with the combination of the 14C-urea breath test (14C-UBT), urease test and histologic examination for the diagnosis of H. pylori (HP) infection. Fifty-three duodenal ulcer patients were studied. All patients were submitted to gastroscopy to detect HP by the urease test, histologic examination and 14C-UBT. To be included in the study the results of the 3 tests had to be concordant. Within one month after admission to the study the patients were submitted to IRIS with breath samples collected before and 30 min after the ingestion of 75 mg 13C-urea dissolved in 200 ml of orange juice. The samples were mailed and analyzed 11.5 (4-21) days after collection. Data were analyzed statistically by the chi-square and Mann-Whitney test and by the Spearman correlation coefficient. Twenty-six patients were HP positive and 27 negative. There was 100% agreement between the IRIS results and the HP status determined by the other three methods. Using a cutoff value of delta-over-baseline (DOB) above 4.0 the IRIS showed a mean value of 19.38 (minimum = 4.2, maximum = 41.3, SD = 10.9) for HP-positive patients and a mean value of 0.88 (minimum = 0.10, maximum = 2.5, SD = 0.71) for negative patients. Using a cutoff value corresponding to 0.800% CO2/weight (kg), the 14C-UBT showed a mean value of 2.78 (minimum = 0.89, maximum = 5.22, SD = 1.18) in HP-positive patients. HP-negative patients showed a mean value of 0.37 (minimum = 0.13, maximum = 0.77, SD = 0.17). IRIS is a low-cost, easy to manage, highly sensitive and specific test for H. pylori detection. Storing and mailing the samples did not interfere with the performance of the test.


Assuntos
Infecções por Helicobacter/diagnóstico , Helicobacter pylori , Espectrofotometria Infravermelho/métodos , Ureia , Adulto , Testes Respiratórios/métodos , Isótopos de Carbono , Radioisótopos de Carbono , Úlcera Duodenal/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
16.
Med Clin (Barc) ; 96(1): 6-10, 1991 Jan 12.
Artigo em Espanhol | MEDLINE | ID: mdl-1850813

RESUMO

We analyze the clinical and histological features of 10 cases of malignant fibrous histiocytoma of soft tissue. Nine belonged to the pleomorphic-verticillate variety and one was myxoid. The initial clinical feature was a palpable mass in all cases except three with retroperitoneal localization, where constitutional symptoms predominated. After therapy (surgery in all, associated with radiotherapy in four), seven patients had local relapse and two had distant metastases. 50% died, with a mean survival of 13 months. We discuss the prognostic factors and the therapeutic approach, with emphasis on aggressive therapy and the need for radical surgery and postoperative adjuvant therapy.


Assuntos
Histiocitoma Fibroso Benigno/patologia , Neoplasias de Tecidos Moles/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Seguimentos , Histiocitoma Fibroso Benigno/diagnóstico , Histiocitoma Fibroso Benigno/terapia , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias de Tecidos Moles/diagnóstico , Neoplasias de Tecidos Moles/terapia
17.
An Med Interna ; 8(8): 387-90, 1991 Aug.
Artigo em Espanhol | MEDLINE | ID: mdl-1768748

RESUMO

Cases of brucellosis with involvement of the nervous system which was diagnosed in the Miguel Servet Hospital during the period 1985-1987 are retrospectively studied. The total quantity of affected patients of brucellosis was 132. Of this quantity, 9 patients (6.8%) had neurological complications under the following clinical forms: epidural abscess (2), meningoencephalitis (1), meningitis (2), encephalitis (1), myelitis (1) and polyradiculitis (2). The most important epidemiological and clinical characteristics are analysed, pointing out the diagnostic difficulties we found when the neurological manifestations are predominant in the brucella infection.


Assuntos
Brucelose/complicações , Doenças do Sistema Nervoso/microbiologia , Adolescente , Adulto , Antibacterianos/uso terapêutico , Brucelose/tratamento farmacológico , Brucelose/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Doenças do Sistema Nervoso/epidemiologia , Estudos Retrospectivos
18.
Forensic Sci Int ; 243: 137-43, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25124884

RESUMO

A simple and sensitive procedure, using n-propanol as internal standard (IS), was developed and validated for the qualitative and quantitative analysis of a group of 11 volatile organic substances with different physicochemical properties (1-butanol, 2-propanol, acetaldehyde, ethyl acetate, acetone, acetonitrile, chloroform, diethyl ether, methanol, toluene and p-xylene) in whole blood, urine and vitreous humor. Samples were prepared by dilution with an aqueous solution of internal standard followed by Headspace Gas Chromatography with a Flame-ionization Detector (HS GC-FID) analysis. Chromatographic separation was performed using two capillary columns with different polarities (DB-ALC2: 30m×0.320mm×1.2µm and DB-ALC1: 30m×0.320mm×1.8µm), thus providing a change in the retention and elution order of volatiles. This dual column confirmation increases the specificity, since the risk of another substance co-eluting at the same time in both columns is very small. The method was linear from 5 to 1000mg/L for toluene and p-xylene, 50-1000mg/L for chloroform, and 50-2000mg/L for the remaining substances, with correlation coefficients of over 0.99 for all compounds. The limits of detection (LOD) ranged 1 to 10mg/L, while the limits of quantification (LOQ) ranged from 2 to 31mg/L. The intra-day precision (CV<6.4%), intermediate precision (CV<7.0%) and accuracy (relative error ±10%) of the method were in conformity with the criteria normally accepted in bioanalytical method validation. The method developed has been applied to forensic cases, with the advantages that it uses a small sample volume and does not require any extraction procedure as it makes use of a headspace injection technique.


Assuntos
Cromatografia Gasosa/métodos , Ionização de Chama , Compostos Orgânicos Voláteis/análise , Humanos , Limite de Detecção , Corpo Vítreo/química
19.
Carbohydr Polym ; 98(1): 152-60, 2013 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-23987329

RESUMO

The -NCO-functionalization of methyl cellulose with HMDI and its application to chemically gel the castor oil is explored in this work by analyzing the influence of functionalization degree on the rheological and thermogravimetric behavior of resulting chemical oleogels. With this aim, different methyl cellulose chemical modifications were achieved by limiting the proportion of HMDI and, subsequently, oleogels were obtained by dispersing these polymers in castor oil and promoting the reaction between those biopolymers and the hydroxyl groups located in the ricinoleic fatty acid chain. -NCO-functionalized methyl cellulose-based oleogels were characterized from themogravimetric and rheological points of view. Suitable thermal resistance and rheological characteristics were found in order to propose these oleogels as promising bio-based alternatives to traditional lubricating greases based on non-renewable resources. In general, -NCO-functionalized methyl cellulose thermally decomposed in three main steps whereas resulting oleogels thermal decomposition takes place in one main single stage which comprises the thermal degradation of both the polymer and the castor oil. Temperature range for thermal degradation is broadened when using highly -NCO-functionalized methyl cellulose. A cross-linked viscoelastic gel was obtained with methyl cellulose functionalized in a relatively low degree (around 6% -NCO molar content). The rheological properties of highly functionalized methyl cellulose-based oleogels evolve during several months of aging, but mainly during the first week, due to the progress of the reaction between -NCO functional groups and castor oil -OH groups. SAOS functions analyzed and oleogel relative elasticity increase with the functionalization degree. Oleogel linear viscoelastic response is also extremely dependent on NCO-functionalized methyl cellulose concentration.


Assuntos
Cianatos/química , Metilcelulose/química , Reologia , Temperatura , Isocianatos , Compostos Orgânicos/química
20.
Toxicon ; 76: 221-4, 2013 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-24140920

RESUMO

Dinophysis sacculus is associated with DSP outbreaks especially in the Mediterranean Sea and is supposed to be mildly toxic based on few toxin results from field samples. First report of LC-MS analysis of D. sacculus cultures from Galicia (NW Spain) showed moderate amounts of OA (7.8 pg cell(-1)) comparable to those found in Dinophysis acuminata from the same region, PTX2 (13.2 pg cell(-1)) and trace amounts of DTX1 (0.8 pg OA equiv. cell(-1)). The contribution of D. sacculus to DSP outbreaks in the Galician Northern Rías should not be underestimated.


Assuntos
Dinoflagellida/química , Toxinas Marinhas/química , Cromatografia Líquida , Dinoflagellida/metabolismo , Monitoramento Ambiental , Humanos , Toxinas Marinhas/isolamento & purificação , Toxinas Marinhas/metabolismo , Espectrometria de Massas , Ácido Okadáico/química , Ácido Okadáico/isolamento & purificação , Ácido Okadáico/metabolismo , Piranos/química , Piranos/isolamento & purificação , Piranos/metabolismo , Intoxicação por Frutos do Mar/etiologia , Espanha
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