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1.
Brief Bioinform ; 22(4)2021 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-33079984

RESUMO

OBJECTIVE: We aimed to identify key susceptibility gene targets in multiple datasets generated from postmortem brains and blood of Parkinson's disease (PD) patients and healthy controls (HC). METHODS: We performed a multitiered analysis to integrate the gene expression data using multiple-gene chips from 244 human postmortem tissues. We identified hub node genes in the highly PD-related consensus module by constructing protein-protein interaction (PPI) networks. Next, we validated the top four interacting genes in 238 subjects (90 sporadic PD, 125 HC and 23 Parkinson's Plus Syndrome (PPS)). Utilizing multinomial logistic regression analysis (MLRA) and receiver operating characteristic (ROC), we analyzed the risk factors and diagnostic power for discriminating PD from HC and PPS. RESULTS: We identified 1333 genes that were significantly different between PD and HCs based on seven microarray datasets. The identified MEturquoise module is related to synaptic vesicle trafficking (SVT) dysfunction in PD (P < 0.05), and PPI analysis revealed that SVT genes PPP2CA, SYNJ1, NSF and PPP3CB were the top four hub node genes in MEturquoise (P < 0.001). The levels of these four genes in PD postmortem brains were lower than those in HC brains. We found lower blood levels of PPP2CA, SYNJ1 and NSF in PD compared with HC, and lower SYNJ1 in PD compared with PPS (P < 0.05). SYNJ1, negatively correlated to PD severity, displayed an excellent power to discriminating PD from HC and PPS. CONCLUSIONS: This study highlights that SVT genes, especially SYNJ1, may be promising markers in discriminating PD from HCs and PPS.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Proteínas do Tecido Nervoso , Doença de Parkinson , Mapas de Interação de Proteínas , Vesículas Sinápticas , Autopsia , Biomarcadores/metabolismo , Feminino , Humanos , Masculino , Proteínas do Tecido Nervoso/biossíntese , Proteínas do Tecido Nervoso/genética , Doença de Parkinson/genética , Doença de Parkinson/metabolismo , Vesículas Sinápticas/genética , Vesículas Sinápticas/metabolismo
2.
Cell Mol Biol (Noisy-le-grand) ; 69(4): 141-146, 2023 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-37329535

RESUMO

The Parkinson's disease is the second most common neurodegenerative disease with different pathological mechanisms at each stage. To investigate Parkinson's disease further, this study was proposed to develop a continuous staging mouse model of Parkinson's disease to reproduce the pathological features of different stages of Parkinson's disease. We successively treated the mice with MPTP, and assessed the behavioral performance of the mice with the open field test and the rotarod test, and detected the aggregation of α-syn and the expression of TH protein in the substantia nigra of the mice with western blot test and immunofluorescence test. The results showed that the mice injected with MPTP for 3 days had no significant behavioral changes, no significant α-syn aggregation, but reduced TH protein expression and 39.5% loss of dopaminergic neurons in the substantia nigra, similar to the performance in the prodromal phase of Parkinson's disease. However, the behavior of the mice continuously treated with MPTP for 14 days was significantly altered, with significant α-syn aggregation, significant reduction in TH protein expression, and 58.1% loss of dopaminergic neurons in the substantia nigra, corresponding to the early clinical stage of Parkinson's disease. In the mice that were exposed to MPTP for 21 days, the motor impairment was more obvious, the α-syn aggregation was more significant, the reduction of TH protein expression was more evident, and the loss of dopaminergic neurons reached 80.5% in the substantia nigra, showing a clinical progression similar to that of Parkinson's disease. Consequently, this study found that continuous treatment of C57/BL6 mice with MPTP for 3, 14 and 21 days could construct mouse models of prodromal, early clinical and clinical progressive stages of Parkinson's disease, respectively, providing a promising experimental model foundation for the study of the different stages of Parkinson's disease.


Assuntos
Doenças Neurodegenerativas , Doença de Parkinson , Animais , Camundongos , Doença de Parkinson/metabolismo , Doenças Neurodegenerativas/metabolismo , Substância Negra/metabolismo , Substância Negra/patologia , Modelos Animais de Doenças , Neurônios Dopaminérgicos/metabolismo , Neurônios Dopaminérgicos/patologia , Camundongos Endogâmicos C57BL
3.
Angew Chem Int Ed Engl ; 62(33): e202304491, 2023 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-37314397

RESUMO

A multi-level architecture formed alternatively by the conformal graphdiyne (GDY) and CuS is well engineered for Li-free cathode. Such a proof-of-concept architecture efficiently integrates the advantages of GDY and produces new functional heterojunctions (sp-C-S-Cu hybridization bond). The layer-by-layer 2D confinement effect successfully avoids structural collapse, the selective transport inhibits the shuttling of active components, and the interfacial sp-C-S-Cu hybridization bond significantly regulates the phase conversion reaction. Such new sp-C-S-Cu hybridization of GDY greatly improves the reaction dynamics and reversibility, and the cathode delivers an energy density of 934 Wh kg-1 and an unattenuated lifespan of 3000 cycles at 1 C. Our results indicate that the GDY-based interface strategy will greatly promote the efficient utilization of the conversion-type cathodes.

4.
Nutr Neurosci ; 24(1): 23-34, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30873919

RESUMO

Objective: We present novel dimensional methods to describe the timing of eating in psychopathology. We focused on the relationship between current mood in bipolar disorder (BD) and the stability of the temporal pattern of daily eating events. Methods: Consenting BD patients (n = 69) from an outpatient, tertiary care clinic completed hourly charts of mood and eating for two weeks. Mood was also evaluated with Montgomery-Åsberg Depression Rating Scale (MADRS) and Young Mania Rating Scale (YMRS). Results: Illustrative displays, or eatograms, enabling visualization of all recorded eating events were used to guide assessment of the temporal structure of eating across the two week assessment period. We computed indices to quantify irregularities in timing of eating, namely IFRQ, ITIM and IINT for the variability of frequency, timing, and interval of eating events, respectively. In this cohort, irregular temporal pattern of eating correlated with hypomanic symptoms (YMRS with IFRQ, Spearman rank order rh = 0.28, p = .019, with ITIM, rh = 0.44, p < .001, and with IINT rh = 0.38, p = .001), but not depressive symptoms or anthropometric measures. Conclusions: Our data suggest a link between the instability of the temporal order of daily eating and mood. The dimensional measures for eating pattern introduced here enable future investigations of correlations with psychopathology.


Assuntos
Transtorno Bipolar/psicologia , Ingestão de Alimentos/psicologia , Mania/psicologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Escalas de Graduação Psiquiátrica
5.
Chem Soc Rev ; 49(24): 9028-9056, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33135701

RESUMO

Facile activation and conversion of small molecules (e.g., H2O, CO2, N2, CH4, and C6H6) into solar fuels or value-added chemicals under mild conditions is an attractive pathway in dealing with the worldwide appeal of energy consumption and the growing demand of industrial feedstocks. Compared with conventional thermo- or electro-catalytic approaches, the protocol of photocatalysis shines light on green and low-cost storage of sunlight in chemical bonds. For instance, artificial photosynthesis is an effective way to split H2O into molecular O2 and H2, thereby storing solar energy in the form of hydrogen fuel. Because of rational tunability in band gaps, charge-carrier dynamics, exposed active sites and catalytic redox activities by tailoring size, composition, morphology, surface, and/or interface property, semiconductor nanocrystals (NCs) emerge as very promising candidates for photo-induced small molecule activation, including H2O splitting, CO2 reduction, N2 fixation, CH4 conversion and chemical bond formation (e.g., S-S, C-C, C-N, C-P, C-O). In this review, we summarize the recent advances in small molecule activation via artificial photosynthesis using semiconductor NCs, especially those consisting of II-VI and III-V elements. Moreover, we highlight the intrinsic advantages of semiconductor NCs in this field and look into the fabrication of prototype devices for large-scale and sustainable small molecule activation to store solar energy in chemical bonds.

6.
J Environ Sci (China) ; 101: 351-360, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33334529

RESUMO

Pyrite is the most abundant sulfide semiconductor mineral with excellent optical properties. However, few reports have investigated its photocatalytic activity because of the low photogenerated carrier separation efficiency. In this work, a Z-scheme FeS2/Fe2O3 composite photocatalyst was fabricated in situ via structural transformation of pyrite through heat treatment. A remarkably enhanced photocatalytic performance was observed over the FeS2/Fe2O3 composite photocatalyst. Compared with the pristine pyrite, the degradation efficiency of carbamazepine (CBZ) reached 65% at the added hexavalent chromium (Cr(Ⅵ)) concentration of 20 mg/L and the Cr(Ⅵ) was nearly completely reduced in the mixed system using FeS2/Fe2O3 within 30 min under simulated solar light irradiation. The enhanced photocatalytic activity can be attributed to the efficient separation and transfer of photogenerated carriers in the FeS2/Fe2O3 composite photocatalyst. This facilitated the generation of •OH, hole (h+) and •O2- species, which participated in the photocatalytic reaction with CBZ. Based on the measurement of the active species and electric properties, a Z-scheme electron transfer pathway was proposed for the FeS2/Fe2O3 composite photocatalyst. This work broadens the application potential of pyrite in environmental remediation.


Assuntos
Carbamazepina , Sulfetos , Catálise , Cromo , Ferro
7.
J Am Chem Soc ; 142(10): 4680-4689, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32066243

RESUMO

Semiconductor quantum dots (QDs) in conjunction with non-noble 3d-metal ions (e.g., Fe3+, Co2+, and Ni2+) have emerged as an extremely efficient, facile, and cost-effective means of solar-driven hydrogen (H2) evolution. However, the exact structural change of the active sites under realistic conditions remains elusive, and the mechanism of H2 evolution behind the remarkable activity is poorly understood. Here, we successfully track the structural variation of the catalytic sites in the typical H2 photogeneration system consisting of CdSe/CdS QDs and 3d-metal ions (i.e., Ni2+ used here). That is, the nickel precursor of Ni(OAc)2 changes to Ni(H2O)62+ in neutral H2O and eventually transforms to Ni(OH)2 nanosheets in alkaline media. Furthermore, the in operando spectroscopic techniques of electron paramagnetic resonance and X-ray absorption spectroscopy reveal the photoinduced transformation of Ni(OH)2 to a defective structure [Nix0/Ni1-x(OH)2], which acts as the real catalytic species of H2 photogeneration. Density functional theory (DFT) calculations further indicate that the surface Ni-vacancies (VNi) on the Ni(OH)2 nanosheets enhance the adsorption and dissociation of H2O molecules to enhance the local proton concentration, while the Ni0 clusters behave as H2-evolution sites, thereby synergistically promoting the activity of H2 photogeneration in alkaline media.

8.
Stroke ; 48(1): 63-69, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27932606

RESUMO

BACKGROUND AND PURPOSE: We aimed to develop and validate a grading scale for predicting 30-day mortality and 90-day functional outcome in patients with primary pontine hemorrhage (PPH). METHODS: We retrospectively reviewed records of consecutive patients with first-ever pontine hemorrhage from 3 teaching hospitals between 2005 and 2012. Independent factors associated with 30-day mortality were identified by logistic regression to establish a risk stratification scale, named the new PPH score. For validation of the new PPH score, we prospectively recruited subjects from 10 units between December 2014 and November 2015. The performance of the new PPH score was presented as discrimination and calibration, measured by area under the curve of the receiver operating characteristic and Hosmer-Lemeshow goodness-of-fit, respectively. RESULTS: Data of 171 patients were available for scale development. The new PPH score consisted of 2 independent factors with individual points assigned as follows: Glasgow Coma Scale score 3 to 4 (=2 points), 5 to 7 (=1 point), and 8 to 15 (=0 point); PPH volume >10 mL (=2 points), 5 to 10 mL (=1 point), and <5 mL (=0 point). An independent cohort of 98 patients was applied as an external validation of the new PPH score. Results showed that the new PPH score was discriminative in predicting both 30-day mortality (area under the curve, 0.902) and 90-day good outcome (area under the curve, 0.927). Furthermore, the new PPH score revealed a good calibration (χ2=1.387; P=0.846) in 30-day mortality prediction. CONCLUSIONS: The new PPH score is simple and reliable in predicting short-term and long-term outcome for PPH patients. CLINICAL TRIAL REGISTRATION: URL: http://www.chictr.org.cn. Unique identifier: ChiCTR-OOC-14005533.


Assuntos
Hemorragias Intracranianas/diagnóstico , Ponte/patologia , Índice de Gravidade de Doença , Adulto , Idoso , Escala de Coma de Glasgow/normas , Humanos , Hemorragias Intracranianas/epidemiologia , Pessoa de Meia-Idade , Estudos Prospectivos , Reprodutibilidade dos Testes , Estudos Retrospectivos
9.
Cryobiology ; 72(2): 141-7, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26802735

RESUMO

This study aimed to identify optimal mild hypothermic (MH) condition that would provide the best protection for neuronal cells undergoing severe ischemia and hypoxia. We also sought to determine if longer exposure to mild hypothermia would confer greater protection to severe ischemia and hypoxia in these cells. We designed a primary neuronal cell model for severe glucose and oxygen deprivation/reoxygenation (OGD/R) to simulate the hypoxic-ischemic condition of patients with severe stroke, trauma, or hypoxic-ischemic encephalopathy. We evaluated the viability of these neurons following 3 h of OGD/R and variable MH conditions including different temperatures and durations of OGD/R exposure. We further explored the effects of the optimal MH condition on several parts which are associated with mitochondrial apoptosis pathway: intracellular calcium, reactive oxygen species (ROS), and mitochondrial transmembrane potential (MTP). The results of this study showed that the apoptosis proportion (AP) and cell viability proportion (CVP) after OGD/R significantly varied depending on which MH condition cells were exposed to (p < 0.001). Further, our findings showed that prolonged MH reduced the neuroprotection to AP and CVP. We also determined that the optimal MH conditions (34 °C for 4.5 h) reduced intracellular calcium, ROS, and recovered MTP. These findings indicate that there is an optimal MH treatment strategy for severely hypoxia-ischemic neurons, prolonged duration might diminish the neuroprotection, and that MH treatment likely initiates neuroprotection by inhibiting the mitochondrial apoptosis pathway.


Assuntos
Hipóxia Celular/fisiologia , Hipotermia Induzida/métodos , Hipotermia/fisiopatologia , Neurônios/citologia , Neuroproteção/fisiologia , Animais , Apoptose/fisiologia , Cálcio/metabolismo , Sobrevivência Celular/fisiologia , Células Cultivadas , Glucose/metabolismo , Hipotermia/metabolismo , Potencial da Membrana Mitocondrial , Mitocôndrias/metabolismo , Modelos Animais , Neurônios/fisiologia , Oxigênio/metabolismo , Ratos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo
10.
Food Res Int ; 188: 114532, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38823889

RESUMO

Luteolin has anti-inflammatory, antioxidant, and anti-tumor functions, but its poor water solubility and stability limit its applications in foods as a functional component. In this study, the nanocomposites loading luteolin (Lut) with soybean protein isolate (SPI), soluble soybean polysaccharide (SSPS) and/or rhamnolipid (Rha) were prepared by layer-by-layer shelf assembly method, and their properties were also evaluated. The results showed that Rha/SPI/Lut had the smallest particle size (206.24 nm) and highest loading ratio (8.03 µg/mg) while Rha/SSPS/SPI/Lut had the highest encapsulation efficiency (82.45 %). Rha interacted with SPI through hydrophobic interactions as the main driving force, while SSPS attached to SPI with only hydrogen bonding. Furthermore, the synergistic effect between Rha and SSPS was observed in Rha/SSPS/SPI/Lut complex, in consequence, it had the best thermal and storage stability, and the slowest release in gastrointestinal digestion. Thus, this approach provided an alternative way for the application of luteolin in functional foods.


Assuntos
Digestão , Luteolina , Tamanho da Partícula , Proteínas de Soja , Luteolina/química , Proteínas de Soja/química , Nanocompostos/química , Polissacarídeos/química , Interações Hidrofóbicas e Hidrofílicas , Glycine max/química , Solubilidade , Alimento Funcional , Trato Gastrointestinal/metabolismo
11.
Mol Neurobiol ; 61(3): 1833-1844, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-37787950

RESUMO

Norepinephrine (NE) is involved in auditory fear conditioning (AFC) in posttraumatic stress disorder (PTSD). However, it is still unclear how it acts on neurons. We aimed to investigate whether the activation of the ß-adrenergic receptor (ß-AR) improves AFC by sensitization of the prelimbic (PL) cortex at the animal, cellular, and molecular levels. In vivo single-cell electrophysiological recording was used to characterize the changes in neurons in the PL cortex after AFC. Then, PL neurons were locally administrated by the ß-AR agonist isoproterenol (ISO), the GABAaR agonist muscimol, or intervened by optogenetic method, respectively. Western blotting and immunohistochemistry were finally used to assess molecular changes. Noise and low-frequency tones induced similar AFC. The expression of ß-ARs in PL cortex neurons was upregulated after fear conditioning. Microinjection of muscimol into the PL cortex blocked the conformation of AFC, whereas ISO injection facilitated AFC. Moreover, PL neurons can be distinguished into two types, with type I but not type II neurons responding to conditioned sound and being regulated by ß-ARs. Our results showed that ß-ARs in the PL cortex regulate conditional fear learning by activating type I PL neurons.


Assuntos
Córtex Pré-Frontal , Receptores Adrenérgicos beta , Animais , Córtex Pré-Frontal/fisiologia , Muscimol , Razão Sinal-Ruído , Isoproterenol/farmacologia , Medo/fisiologia
12.
Mol Ther Oncol ; 32(2): 200790, 2024 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-38595980

RESUMO

N5-methylcytosine (m5C) methylation modification plays a crucial role in the epigenetic mechanisms underlying tumorigenesis, aggressiveness, and malignancy in diffuse glioma. Our study aimed to develop a novel prognostic risk-scoring system to assess the impact of m5C modification in glioma patients. Initially, we identified two distinct m5C clusters based on the expression level of m5C regulators in The Cancer Genome Atlas glioblastoma (TCGA-GBM) dataset. Differentially expressed genes (DEGs) between the two m5C cluster groups were determined. Utilizing these m5C regulation-related DEGs, we classified glioma patients into three gene cluster groups: A, B, and C. Subsequently, an m5C scoring system was developed through a univariate Cox regression model, quantifying the m5C modification patterns utilizing six DEGs associated with disease prognosis. The resulting scoring system allowed us to categorize patients into high- or low-risk groups based on their m5C scores. In test (TCGA-GBM) and validation (Chinese Glioma Genome Atlas [CGGA]-1018 and CGGA-301) datasets, glioma patients with a higher m5C score consistently exhibited shorter survival durations, fewer isocitrate dehydrogenase (IDH) mutations, less 1p/19q codeletion and higher World Health Organization (WHO) grades. Additionally, distinct immune cell infiltration characteristics were observed among different m5C cluster groups and risk groups. Our study developed a novel prognostic scoring system based on m5C modification patterns for glioma patients, complementing existing molecular classifications and providing valuable insights into prognosis for glioma patients.

15.
J Plant Physiol ; 280: 153896, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36525837

RESUMO

Heavy metal stress affects the quality of medicinal plants, and rare earth elements can effectively alleviate heavy metal stress. In this paper, we investigated the effects of rare earth element cerium (0, 5, 10, 20, 40, 80, and 160 mg/L) on the physiological and medicinal components of Dendrobium nobile Lindl. under copper (200 mg/L) stress. The results revealed that cerium (Ce) had a good alleviating effect on copper (Cu) stress, low concentrations of Ce (10-20 mg/L) significantly improved the resistance and medicinal qualities of the plant such as polysaccharide, polyphenol and flavonoid, it also increased the content of photosynthetic pigment, proline, soluble sugar and soluble protein of D. nobile Lindl., effectively balance the osmotic pressure and the generation and removal of reactive oxygen species in the plant, thereby the toxic effect of copper on D. nobile Lindl. is alleviated. From the point of view of the treatment time when the optimal relieving concentration appeared, the optimal concentration for relieving antioxidant enzyme activity all appeared at the treatment time of 10 d, the optimum concentrations of other indicators all appeared at the treatment time of 15 d. Overall, this study suggests that the optimum level of Ce (10-20 mg/L) might be promising for alleviating the adverse impacts of copper stress and promoting the accumulation of medicinal components in D. nobile Lindl.


Assuntos
Cério , Dendrobium , Plantas Medicinais , Cobre/toxicidade , Cério/farmacologia , Extratos Vegetais/farmacologia , Antioxidantes
16.
Behav Brain Res ; 452: 114569, 2023 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-37419331

RESUMO

This study aimed to explore the role of SYNJ1 in Parkinson's disease (PD) and its potential as a neuroprotective factor. We found that SYNJ1 was decreased in the SN and striatum of hSNCA*A53T-Tg and MPTP-induced mice compared to normal mice, associated with motor dysfunction, increased α-synuclein and decreased tyrosine hydroxylase. To investigate its neuroprotective effects, SYNJ1 expression was upregulated in the striatum of mice through injection of the rAdV-Synj1 virus into the striatum, which resulted in the rescue of behavioral deficiencies and amelioration of pathological changes. Subsequently, transcriptomic sequencing, bioinformatics analysis and qPCR were conducted in SH-SY5Y cells following SYNJ1 gene knockdown to identify its downstream pathways, which revealed decreased expression of TSP-1 involving extracellular matrix pathways. The virtual protein-protein docking further suggested a potential interaction between the SYNJ1 and TSP-1 proteins. This was followed by the identification of a SYNJ1-dependent TSP-1 expression model in two PD models. The coimmunoprecipitation experiment verified that the interaction between SYNJ1 and TSP-1 was attenuated in 11-month-old hSNCA*A53T-Tg mice compared to normal controls. Our findings suggest that overexpression of SYNJ1 may protect hSNCA*A53T-Tg and MPTP-induced mice by upregulating TSP-1 expression, which is involved in the extracellular matrix pathways. This suggests that SYNJ1 could be a potential therapeutic target for PD, though more research is needed to understand its mechanism.


Assuntos
Neuroblastoma , Fármacos Neuroprotetores , Doença de Parkinson , Camundongos , Humanos , Animais , Doença de Parkinson/genética , Doença de Parkinson/tratamento farmacológico , Trombospondina 1 , Neuroblastoma/tratamento farmacológico , alfa-Sinucleína/genética , alfa-Sinucleína/metabolismo , Fármacos Neuroprotetores/farmacologia , Neuroproteção , Camundongos Endogâmicos C57BL , Modelos Animais de Doenças
17.
Am J Obstet Gynecol MFM ; 5(1): 100790, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36377092

RESUMO

BACKGROUND: Genome-wide noninvasive prenatal testing identifies several rare autosomal trisomies in the general obstetrical population, but its use is questioned by its low positive predictive value. Furthermore, the origin of rare autosomal trisomies and the clinical effect of reporting them has not been sufficiently investigated. In addition, professional societies express their need for data assessing the clinical use of genome-wide noninvasive prenatal testing for rare autosomal trisomies for years. OBJECTIVE: This study aimed to investigate the origin of rare autosomal trisomies and the clinical effect of disclosing rare autosomal trisomies in clinical settings. STUDY DESIGN: Women who received noninvasive prenatal testing between March 2021 and March 2022 were prospectively enrolled. Clinical follow-up and cytogenetic and molecular investigations were performed. Posthoc analysis was performed to investigate the association between placental mosaicism and clinical outcomes. RESULTS: Overall, 154 rare autosomal trisomies were identified in 89,242 pregnancies (0.17%) through noninvasive prenatal testing. In the 120 cases in which cytogenetic and molecular investigations were carried out, the rare autosomal trisomies were found to originate from true fetal mosaicism (n=5), uniparental disomy (n=5), maternal mosaic trisomy (n=3), maternal malignancy (n=1), and confined placental mosaicism (n=106). Clinical follow-up showed that 40% of all rare autosomal trisomy cases had adverse perinatal outcomes. In women with false-positive noninvasive prenatal testing results originating from confined placental mosaicism, the frequency of adverse perinatal outcomes was 26%. More importantly, the placental mosaicism ratio revealed by noninvasive prenatal testing was significantly higher in women who experienced adverse perinatal outcomes than those who did not (0.688 vs 0.332; P<.001). CONCLUSION: Women with noninvasive prenatal testing results indicative of rare autosomal trisomies are at risk of adverse perinatal outcomes, and that risk can be stratified using chromosomes and the mosaicism ratio revealed by noninvasive prenatal testing. Our data are valuable for obstetrical caregivers advising a patient with a noninvasive prenatal testing result indicative of a rare autosomal trisomy and a false-positive diagnosis and for managing risks during pregnancy.


Assuntos
Teste Pré-Natal não Invasivo , Trissomia , Feminino , Gravidez , Humanos , Trissomia/diagnóstico , Trissomia/genética , Trissomia/patologia , Placenta/patologia , Mosaicismo , Cromossomos
18.
J Biomed Biotechnol ; 2012: 803930, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23193366

RESUMO

The study explored a modified primary culture system for fetal rat cortical neurons. Day E18 embryos from pregnant Sprague Dawley rats were microdissected under a stereoscope. To minimize enzymatic damage to the cultured neurons, we applied a sequential digestion protocol using papain and Dnase I. The resulting sifted cell suspension was seeded at a density of 50,000 cells per cm(2) onto 0.1 mg/mL L-PLL-covered vessels. After a four-hour incubation in high-glucose Dulbecco's Modified Eagle's Medium (HG-DMEM) to allow the neurons to adhere, the media was changed to neurobasal medium that was refreshed by changing half of the volume after three days followed by a complete medium change every week. The cells displayed progressively robust neurite extension, and nonneuronal-like cells could barely be detected by five days in vitro (DIV); cell growth was still substantial at 14 DIV. Neurons were identified by ß-tubulin III immunofluorescence, and neuronal purity within the cultures was assessed at over 95% by both flow cytometry and by dark-field counting of ß-tubulin III-positive cells. These results suggest that the protocol was successful and that the high purity of neurons in this system could be used as the basis for generating various cell models of neurological disease.


Assuntos
Técnicas de Cultura de Células/métodos , Córtex Cerebral/citologia , Córtex Cerebral/embriologia , Feto/citologia , Neurônios/citologia , Animais , Células Cultivadas , Craniotomia , Dissecação , Feminino , Feto/irrigação sanguínea , Imunofluorescência , Indóis/metabolismo , Neurônios/metabolismo , Ratos , Ratos Sprague-Dawley , Crânio/cirurgia , Tubulina (Proteína)/metabolismo
19.
Chemosphere ; 303(Pt 1): 134863, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35561759

RESUMO

Spherical cerium dioxide (CeO2-S) nanoparticles were successfully prepared using a solvothermal method, and their performances in catalytic oxidation reactions were studied. The CeO2-S catalyst showed superior low-temperature catalytic activity for styrene removal (T90 = 118 °C, GHSV = 18,000 h-1) compared with commercial CeO2. The characterization results showed that there were numerous oxygen defects in CeO2-S that were key to its catalytic performance at low temperatures, high redox properties, and high adsorption capacity for the reaction gases (O2 and styrene). Moreover, the catalytic performance of CeO2-S was highly stable (132 h), and the particles were reusable. FTIR and in-situ DRIFTS results showed that the type of intermediates formed during the oxidation of styrene determined the CeO2 catalytic stability, and the main intermediates were bidentate carbonate species that accumulated on the surface of deactivated CeO2-S and were not thermally stable. Moreover, the soft carbon that also deposited on CeO2-S during the reaction was easily decomposed at higher temperatures. The role of the oxygen vacancies on the CeO2-S catalyst was further revealed by correlating the concentration of oxygen vacancies and the accumulation of coke on the catalyst surface.

20.
Chemosphere ; 307(Pt 4): 136199, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36030937

RESUMO

Herein, a heterogeneous photo-Fenton and photo-catalytic system was constructed using oxide pyrite (FeS2/Fe2O3) mineral and organic acids including tartaric acid (TA), ascorbic acid (AA), and citric acid (CA). In the proposed system, FeS2/Fe2O3 can be successfully activated through irradiation to generate photogenerated carriers, which generated H2O2in-situ through the reduction reactions between e- and O2. The addition of organic acids enhanced the dissolution of iron from FeS2/Fe2O3. Based on the iron and in-situ generated H2O2, •OH was produced through a photo-Fenton reaction. Furthermore, h+, e-, and •O2-, which were generated through the photo-catalytic activation of FeS2/Fe2O3, also played a certain role in the degradation of carbamazepine (CBZ). Therefore, the synergistic photo-Fenton and photo-catalytic reaction improved the degradation of CBZ, with the degradation efficiencies of 86%, 62%, and 68% in FeS2/Fe2O3/TA, FeS2/Fe2O3/AA, and FeS2/Fe2O3/CA systems, respectively. This investigation provides an innovative strategy for the removal of organic pollutants using natural minerals.


Assuntos
Poluentes Ambientais , Peróxido de Hidrogênio , Ácido Ascórbico , Carbamazepina , Ácido Cítrico , Ferro , Minerais , Óxidos
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