HoloTile light engine: new digital holographic modalities and applications.

HoloTile is a patented computer generated holography approach with the aim of reducing the speckle noise caused by the overlap of the non-trivial physical extent of the point spread function in Fourier holographic systems from adjacent frequency components. By combining tiling of phase-only of rapidly generated sub-holograms with a PSF-shaping phase profile, each frequency component-or output 'pixel'- in the Fourier domain is shaped to a desired non-overlapping profile. In this paper, we show the high-resolution, speckle-reduced reconstructions that can be achieved with HoloTile, as well as present new HoloTile modalities, including an expanded list of PSF options with new key properties. In addition, we discuss numerous applications for which HoloTile, its rapid hologram generation, and the new PSF options may be an ideal fit, including optical trapping and manipulation of particles, volumetric additive printing, information transfer and quantum communication.

Phase-only steerable photonic nanojets.

We demonstrate numerically the feasibility of axial and angular control of the position of a photonic nanojet (PNJ) by lossless phase-only modulation of a fixed Gaussian beam illuminating a fixed 2D circular homogeneous dielectric micro-lens. We furthermore demonstrate that our phase-only modality can be used to calibrate and improve the confinement of PNJ generation.

Natural convection induced by an optically fabricated and actuated microtool with a thermoplasmonic disk.

Two-photon polymerization was employed for fabricating microtools amenable to optical trapping and manipulation. A disk feature was included as part of the microtools and further functionalized by electron-beam deposition. The nanostructured gold layer on the disk facilitates off-resonant plasmonic heating upon illumination with a laser beam. As a consequence, natural convection characterized by the typical toroidal shape resembling that of Rayleigh-Bénard flow can be observed. A velocity of several µm·s-1 is measured for 2 µm microspheres dispersed in the surroundings of the microtool. To the best of our knowledge, this is the first time that thermoplasmonic-induced natural convection is experimentally demonstrated using a mobile heat source.

GPC light shaping a supercontinuum source.

Generalized Phase Contrast (GPC) is a versatile tool for efficiently rerouting and managing photon energy into speckle-free contiguous spatial light distributions. We have previously shown theoretically and numerically that a GPC Light Shaper shows robustness to shift in wavelength and can maintain both projection length scale and high efficiency over a range [0.75λ(0); 1.5λ(0)] with λ(0) as the characteristic design wavelength. With this performance across multiple wavelengths and the recent availability of tabletop supercontinuum lasers, GPC light shaping opens the possibility for creatively incorporating various multi-wavelength approaches into spatially shaped excitations that can enable new broadband light applications. We verify this new approach using a supercontinuum light source, interfaced with a compact GPC light shaper. Our experiments give ~70% efficiency, ~3x intensity gain, and ~85% energy savings, limited, however, by the illumination equipment, but still in very good agreement with theoretical and numerical predictions.

Dynamic diffraction-limited light-coupling of 3D-maneuvered wave-guided optical waveguides.

We have previously proposed and demonstrated the targeted-light delivery capability of wave-guided optical waveguides (WOWs). As the WOWs are maneuvered in 3D space, it is important to maintain efficient light coupling through the waveguides within their operating volume. We propose the use of dynamic diffractive techniques to create diffraction-limited spots that will track and couple to the WOWs during operation. This is done by using a spatial light modulator to encode the necessary diffractive phase patterns to generate the multiple and dynamic coupling spots. The method is initially tested for a single WOW and we have experimentally demonstrated dynamic tracking and coupling for both lateral and axial displacements.

GPC light shaper for speckle-free one- and two-photon contiguous pattern excitation.

Generalized Phase Contrast (GPC) is an efficient method for generating speckle-free contiguous optical distributions useful in diverse applications such as static beam shaping, optical manipulation and recently, for excitation in two-photon optogenetics. To fully utilize typical Gaussian lasers in such applications, we analytically derive conditions for photon efficient light shaping with GPC. When combined with the conditions for optimal contrast developed in previous works, our analysis further simplifies GPC's implementation. The results of our analysis are applied to practical illumination shapes, such as a circle and different rectangles commonly used in industrial or commercial applications. We also show simple and efficient beam shaping of arbitrary shapes geared towards biophotonics research and other contemporary applications. Optimized GPC configurations consistently give ~84% efficiency and ~3x intensity gain. Assessment of the energy savings when comparing to conventional amplitude masking show that ~93% of typical energy losses are saved with optimized GPC configurations.

GPC light shaper: static and dynamic experimental demonstrations.

Generalized Phase Contrast (GPC) is an efficient method for generating speckle-free contiguous optical distributions useful in diverse applications such as static beam shaping, optical manipulation and, recently, for excitation in two-photon optogenetics. GPC allows efficient utilization of typical Gaussian lasers in such applications using binary-only phase modulation. In this work, we experimentally verify previously derived conditions for photon-efficient light shaping with GPC [Opt. Express22(5), 5299 (2014)]. We demonstrate a compact implementation of GPC for creating practical illumination shapes that can find use in light-efficient industrial or commercial applications. Using a dynamic spatial light modulator, we also show simple and efficient beam shaping of reconfigurable shapes geared towards materials processing, biophotonics research and other contemporary applications. Our experiments give ~80% efficiency, ~3x intensity gain, and ~90% energy savings which are in good agreement with previous theoretical estimations.

Scanless two-photon excitation of channelrhodopsin-2.

Light-gated ion channels and pumps have made it possible to probe intact neural circuits by manipulating the activity of groups of genetically similar neurons. What is needed now is a method for precisely aiming the stimulating light at single neuronal processes, neurons or groups of neurons. We developed a method that combines generalized phase contrast with temporal focusing (TF-GPC) to shape two-photon excitation for this purpose. The illumination patterns are generated automatically from fluorescence images of neurons and shaped to cover the cell body or dendrites, or distributed groups of cells. The TF-GPC two-photon excitation patterns generated large photocurrents in Channelrhodopsin-2-expressing cultured cells and neurons and in mouse acute cortical slices. The amplitudes of the photocurrents can be precisely modulated by controlling the size and shape of the excitation volume and, thereby, be used to trigger single action potentials or trains of action potentials.

Matched filtering Generalized Phase Contrast using binary phase for dynamic spot- and line patterns in biophotonics and structured lighting.

This work discusses the use of matched filtering Generalized Phase Contrast (mGPC) as an efficient and cost-effective beam shaper for applications such as in biophotonics, optical micromanipulation, microscopy and two-photon polymerization. The theoretical foundation of mGPC is described as a combination of Generalized Phase Contrast and phase-only correlation. Such an analysis makes it convenient to optimize an mGPC system for different setup conditions. Results showing binary-only phase generation of dynamic spot arrays and line patterns are presented.

Optical forces through guided light deflections.

Optical trapping and manipulation typically relies on shaping focused light to control the optical force, usually on spherical objects. However, one can also shape the object to control the light deflection arising from the light-matter interaction and, hence, achieve desired optomechanical effects. In this work we look into the object shaping aspect and its potential for controlled optical manipulation. Using a simple bent waveguide as example, our numerical simulations show that the guided deflection of light efficiently converts incident light momentum into optical force with one order-of-magnitude improvement in the efficiency factor relative to a microbead, which is comparable to the improvement expected from orthogonal deflection with a perfect mirror. This improvement is illustrated in proof-of-principle experiments demonstrating the optical manipulation of two-photon polymerized waveguides. Results show that the force on the waveguide exceeds the combined forces on spherical trapping handles. Furthermore, it shows that static illumination can exert a constant force on a moving structure, unlike the position-dependent forces from harmonic potentials in conventional trapping.

Diffractive generalized phase contrast for adaptive phase imaging and optical security.

We analyze the properties of Generalized Phase Contrast (GPC) when the input phase modulation is implemented using diffractive gratings. In GPC applications for patterned illumination, the use of a dynamic diffractive optical element for encoding the GPC input phase allows for on-the-fly optimization of the input aperture parameters according to desired output characteristics. For wavefront sensing, the achieved aperture control opens a new degree of freedom for improving the accuracy of quantitative phase imaging. Diffractive GPC input modulation also fits well with grating-based optical security applications and can be used to create phase-based information channels for enhanced information security.

Matched-filtering generalized phase contrast using LCoS pico-projectors for beam-forming.

We report on a new beam-forming system for generating high intensity programmable optical spikes using so-called matched-filtering Generalized Phase Contrast (mGPC) applying two consumer handheld pico-projectors. Such a system presents a low-cost alternative for optical trapping and manipulation, optical lattices and other beam-shaping applications usually implemented with high-end spatial light modulators. Portable pico-projectors based on liquid crystal on silicon (LCoS) devices are used as binary phase-only spatial light modulators by carefully setting the appropriate polarization of the laser illumination. The devices are subsequently placed into the object and Fourier plane of a standard 4f-setup according to the mGPC spatial filtering configuration. Having a reconfigurable spatial phase filter, instead of a fixed and fabricated one, allows the beam shaper to adapt to different input phase patterns suited for different requirements. Despite imperfections in these consumer pico-projectors, the mGPC approach tolerates phase aberrations that would have otherwise been hard to overcome by standard phase projection.

Optical twists in phase and amplitude.

Light beams with helical phase profile correspond to photons having orbital angular momentum (OAM). A Laguerre-Gaussian (LG) beam is an example where its helical phase sets a phase-singularity at the optical axis and forms a ring-shaped transverse amplitude profile. Here, we describe a unique beam where both phase and amplitude express a helical profile as the beam propagates in free space. Such a beam can be accurately referred to as an optical twister. We characterize optical twisters and demonstrate their capacity to induce spiral motion on particles trapped along the twisters' path. Unlike LG beams, the far field projection of the twisted optical beam maintains a high photon concentration even at higher values of topological charge. Optical twisters have therefore profound applications to fundamental studies of light and atoms such as in quantum entanglement of the OAM, toroidal traps for cold atoms and for optical manipulation of microscopic particles.

Experimental demonstration of Generalized Phase Contrast based Gaussian beam-shaper.

We report the first experimental demonstration of Gaussian beam-shaping based on the Generalized Phase Contrast (GPC) approach. We show that, when using a dynamic spatial light modulator (SLM), this approach can rapidly generate arbitrarily shaped beams. Moreover, we demonstrate that low-cost binary-phase optics fabricated using photolithography and chemical etching techniques can replace the SLM in static and high power beam shaping applications. The design parameters for the binary-phase elements of the module are chosen according to the results of our previously conducted analysis and numerical demonstrations [Opt. Express 15, 11971 (2007)]. Beams with a variety of cross-sections such as circular, rectangular and square, with near flat-top intensity distributions are demonstrated. GPC-based beam shaping is inherently speckle-free and the shaped beams maintain a flat output phase. The non-absorbing components used in this beam-shaping approach have a high-damage-threshold and are thus ideally suited for high power applications.

Single-plane multiple speckle pattern phase retrieval using a deformable mirror.

A design for a single-plane multiple speckle pattern phase retrieval technique using a deformable mirror (DM) is analyzed within the formalism of complex ABCD-matrices, facilitating its use in conjunction with dynamic wavefronts. The variable focal length DM positioned at a Fourier plane of a lens comprises the adaptive optical (AO) system that replaces the time-consuming axial displacements in the conventional free-space multiple plane setup. Compared with a spatial light modulator, a DM has a smooth continuous surface which avoids pixelation, pixel cross-talk and non-planarity issues. The calculated distances for the proposed AO-system are evaluated experimentally using the conventional free-space phase retrieval setup. Two distance ranges are investigated depending on whether the measurement planes satisfy the Nyquist detector sampling condition or not. It is shown numerically and experimentally that speckle patterns measured at the non-Nyquist range still yield good reconstructions. A DM with a surface height of 25 microns and an aperture diameter of 5.2 mm may be used to reconstruct spherical phase patterns with 50-micron fringe spacing.

Dynamic axial stabilization of counter-propagating beam-traps with feedback control.

Optical trapping in a counter-propagating (CP) beam-geometry provides unique advantages in terms of working distance, aberration requirements and intensity hotspots. However, its axial performance is governed by the wave propagation of the opposing beams, which can limit the practical geometries. Here we propose a dynamic method for controlling axial forces to overcome this constraint. The technique uses computer-vision object tracking of the axial position, in conjunction with software-based feedback, for dynamically stabilizing the axial forces. We present proof-of-concept experiments showing real-time rapid repositioning coupled with a strongly enhanced axial trapping for a plurality of particles of varying sizes. We also demonstrate the technique's adaptability for real-time reconfigurable feedback-trapping of a dynamically growing structure that mimics a continuously dividing cell colony. Advanced implementation of this feedback-driven approach can help make CP-trapping resistant to a host of perturbations such as laser fluctuations, mechanical vibrations and other distortions emphasizing its experimental versatility.

Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies.

Cellular development is highly influenced by the surrounding microenvironment. We propose user-reconfigurable microenvironments and bio-compatible scaffolds as an approach for understanding cellular development processes. We demonstrate a model platform for constructing versatile microenvironments by fabricating morphologically complex microstructures by two-photon polymerization (2PP) and then assembling these archetypal building blocks into various configurations using multiple, real-time configurable counterpropagating-beam (CB) traps. The demonstrated capacity for handling feature-rich microcomponents may be further developed into a generalized microassembly platform.

Optical catapulting of microspheres in mucus models-toward overcoming the mucus biobarrier.

The generalized phase contrast method is employed as an efficient "phase-only" laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-µm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. However, velocities of up to 95 µm · s - 1 are demonstrated in a 5% mucin simple mucus model using our catapulting system. This study contributes to understanding and overcoming the challenges of optical manipulation in mucus models. This paves the way for efficient optical manipulation of three-dimensional-printed light-controlled microtools with the ability to penetrate the mucus biobarrier for in vitro drug-delivery studies.

Multi-wavelength spatial light shaping using generalized phase contrast.

The generalized phase contrast method (GPC) is a versatile tool for efficiently rerouting photon energy into desired spatial distributions. We demonstrate that GPC-based patterned projection shows robustness to shift in wavelength and can maintain the projection length scale and high efficiency over a range [0.75lambda(0);1.5lambda(0)] with lambda(0) as the characteristic design wavelength. The GPC has the capacity to combine multiple wavelengths along the same optical path and to efficiently redirect them into desired distributions such as for array illumination, beam shaping, or grayscale image projection. This opens the possibility for creatively incorporating various multi-wavelength approaches into patterned illumination that can enable new broad-band optical applications.

Gaussian to uniform intensity shaper based on generalized phase contrast.

We show that the generalized phase contrast method (GPC) can be used as a versatile tool for shaping an incident Gaussian illumination into arbitrary lateral beam profiles with uniform intensity. This energy-efficient technique shapes the beam by redistributing light from designated dark regions and homogenizes the beam by redistributing excess energy from the center to the edges. Results from numerical experiments show efficiencies around 84% for various profiles with minimal intensity inhomogeneities.