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1.
PLoS Pathog ; 14(8): e1007282, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30157283

RESUMO

Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral silencing suppressors to counter this plant antiviral response. The geminivirus-encoded C4 protein has been identified as a gene silencing suppressor, but the underlying mechanism of action has not been characterized. Here, we report that Cotton Leaf Curl Multan virus (CLCuMuV) C4 protein interacts with S-adenosyl methionine synthetase (SAMS), a core enzyme in the methyl cycle, and inhibits SAMS enzymatic activity. By contrast, an R13A mutation in C4 abolished its capacity to interact with SAMS and to suppress SAMS enzymatic activity. Overexpression of wild-type C4, but not mutant C4R13A, suppresses both transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS). Plants infected with CLCuMuV carrying C4R13A show decreased levels of symptoms and viral DNA accumulation associated with enhanced viral DNA methylation. Furthermore, silencing of NbSAMS2 reduces both TGS and PTGS, but enhanced plant susceptibility to two geminiviruses CLCuMuV and Tomato yellow leaf curl China virus. These data suggest that CLCuMuV C4 suppresses both TGS and PTGS by inhibiting SAMS activity to enhance CLCuMuV infection in plants.


Assuntos
Begomovirus/patogenicidade , Inativação Gênica , Metionina Adenosiltransferase/metabolismo , Interferência de RNA , Proteínas Virais/metabolismo , Begomovirus/metabolismo , Regulação para Baixo/genética , Regulação da Expressão Gênica de Plantas , Interações Hospedeiro-Patógeno/genética , Metionina Adenosiltransferase/genética , Plantas Geneticamente Modificadas , Ligação Proteica , Nicotiana/genética , Nicotiana/metabolismo , Transcrição Gênica , Proteínas Virais/fisiologia
2.
PLoS Pathog ; 12(6): e1005668, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27315204

RESUMO

Viruses interfere with and usurp host machinery and circumvent defense responses to create a suitable cellular environment for successful infection. This is usually achieved through interactions between viral proteins and host factors. Geminiviruses are a group of plant-infecting DNA viruses, of which some contain a betasatellite, known as DNAß. Here, we report that Cotton leaf curl Multan virus (CLCuMuV) uses its sole satellite-encoded protein ßC1 to regulate the plant ubiquitination pathway for effective infection. We found that CLCuMu betasatellite (CLCuMuB) ßC1 interacts with NbSKP1, and interrupts the interaction of NbSKP1s with NbCUL1. Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants. ßC1 impairs the integrity of SCFCOI1 and the stabilization of GAI, a substrate of the SCFSYL1 to hinder responses to jasmonates (JA) and gibberellins (GA). Moreover, JA treatment reduces viral accumulation and symptoms. These results suggest that CLCuMuB ßC1 inhibits the ubiquitination function of SCF E3 ligases through interacting with NbSKP1s to enhance CLCuMuV infection and symptom induction in plants.


Assuntos
Nicotiana/virologia , Doenças das Plantas/virologia , Proteínas Ligases SKP Culina F-Box/metabolismo , Proteínas Virais/metabolismo , Begomovirus , Imunoprecipitação , Microscopia de Fluorescência , Plantas Geneticamente Modificadas , Reação em Cadeia da Polimerase , Técnicas do Sistema de Duplo-Híbrido , Ubiquitinação
3.
J Bacteriol ; 198(1): 127-37, 2016 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-26324450

RESUMO

UNLABELLED: Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. The obligate predatory bacterium Bdellovibrio bacteriovorus has a peculiar dimorphic life cycle, in which a phenotypic transition from a free-living attack phase (AP) to a sessile, intracellular predatory growth phase (GP) is tightly regulated by specific c-di-GMP diguanylate cyclases. B. bacteriovorus also bears one of the largest complement of defined effectors, almost none of known functions, suggesting that additional proteins may be involved in c-di-GMP signaling. In order to uncover novel c-di-GMP effectors, a c-di-GMP capture-compound mass-spectroscopy experiment was performed on wild-type AP and host-independent (HI) mutant cultures, the latter serving as a proxy for wild-type GP cells. Eighty-four proteins were identified as candidate c-di-GMP binders. Of these proteins, 65 did not include any recognized c-di-GMP binding site, and 3 carried known unorthodox binding sites. Putative functions could be assigned to 59 proteins. These proteins are included in metabolic pathways, regulatory circuits, cell transport, and motility, thereby creating a potentially large c-di-GMP network. False candidate effectors may include members of protein complexes, as well as proteins binding nucleotides or other cofactors that were, respectively, carried over or unspecifically interacted with the capture compound during the pulldown. Of the 84 candidates, 62 were found to specifically bind the c-di-GMP capture compound in AP or in HI cultures, suggesting c-di-GMP control over the whole-cell cycle of the bacterium. High affinity and specificity to c-di-GMP binding were confirmed using microscale thermophoresis with a hypothetical protein bearing a PilZ domain, an acyl coenzyme A dehydrogenase, and a two-component system response regulator, indicating that additional c-di-GMP binding candidates may be bona fide novel effectors. IMPORTANCE: In this study, 84 putative c-di-GMP binding proteins were identified in B. bacteriovorus, an obligate predatory bacterium whose lifestyle and reproduction are dependent on c-di-GMP signaling, using a c-di-GMP capture compound precipitation approach. This predicted complement covers metabolic, energy, transport, motility and regulatory pathways, and most of it is phase specific, i.e., 62 candidates bind the capture compound at defined modes of B. bacteriovorus lifestyle. Three of the putative binders further demonstrated specificity and high affinity to c-di-GMP via microscale thermophoresis, lending support for the presence of additional bona fide c-di-GMP effectors among the pulled-down protein repertoire.


Assuntos
Proteínas de Bactérias/metabolismo , Bdellovibrio/fisiologia , GMP Cíclico/análogos & derivados , Regulação Bacteriana da Expressão Gênica/fisiologia , Redes Reguladoras de Genes/fisiologia , Proteínas de Bactérias/genética , GMP Cíclico/genética , GMP Cíclico/metabolismo , Ligação Proteica , Transdução de Sinais
4.
J Virol ; 86(24): 13241-52, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23015709

RESUMO

The whitefly Bemisia tabaci (Gennadius) is a major cosmopolitan pest capable of feeding on hundreds of plant species and transmits several major plant viruses. The most important and widespread viruses vectored by B. tabaci are in the genus Begomovirus, an unusual group of plant viruses owing to their small, single-stranded DNA genome and geminate particle morphology. B. tabaci transmits begomoviruses in a persistent circulative nonpropagative manner. Evidence suggests that the whitefly vector encounters deleterious effects following Tomato yellow leaf curl virus (TYLCV) ingestion and retention. However, little is known about the molecular and cellular basis underlying these coevolved begomovirus-whitefly interactions. To elucidate these interactions, we undertook a study using B. tabaci microarrays to specifically describe the responses of the transcriptomes of whole insects and dissected midguts following TYLCV acquisition and retention. Microarray, real-time PCR, and Western blot analyses indicated that B. tabaci heat shock protein 70 (HSP70) specifically responded to the presence of the monopartite TYLCV and the bipartite Squash leaf curl virus. Immunocapture PCR, protein coimmunoprecipitation, and virus overlay protein binding assays showed in vitro interaction between TYLCV and HSP70. Fluorescence in situ hybridization and immunolocalization showed colocalization of TYLCV and the bipartite Watermelon chlorotic stunt virus virions and HSP70 within midgut epithelial cells. Finally, membrane feeding of whiteflies with anti-HSP70 antibodies and TYLCV virions showed an increase in TYLCV transmission, suggesting an inhibitory role for HSP70 in virus transmission, a role that might be related to protection against begomoviruses while translocating in the whitefly.


Assuntos
Begomovirus/fisiologia , Proteínas de Choque Térmico HSP70/fisiologia , Hemípteros/fisiologia , Animais , Sequência de Bases , Western Blotting , Primers do DNA , Eletroforese em Gel de Poliacrilamida , Etiquetas de Sequências Expressas , Proteínas de Choque Térmico HSP70/genética , Hemípteros/metabolismo , Hemípteros/virologia , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase em Tempo Real
5.
Plant Mol Biol ; 80(3): 273-87, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22843056

RESUMO

To discover genes involved in tomato resistance to Tomato yellow leaf curl virus (TYLCV), we previously compared cDNA libraries from susceptible (S) and resistant (R) tomato lines. Among the genes preferentially expressed in R plants and upregulated by TYLCV infection was a gene encoding a lipocalin-like protein. This gene was termed Solanum lycopersicum virus resistant/susceptible lipocalin (SlVRSLip). The SlVRSLip structural gene sequence of R and S plants was identical. SlVRSLip was expressed in leaves during a 15-day window starting about 40 days after sowing (20 days after planting). SlVRSLip was upregulated by Bemisia tabaci (the TYLCV vector) feeding on R plant leaves, and even more strongly upregulated following whitefly-mediated TYLCV inoculation. Silencing of SlVRSLip in R plants led to the collapse of resistance upon TYLCV inoculation and to a necrotic response along the stem and petioles accompanied by ROS production. Contrary to previously identified tomato lipocalin gene DQ222981, SlVRSLip was not regulated by cold, nor was it regulated by heat or salt. The expression of SlVRSLip was inhibited in R plants in which the hexose transporter gene LeHT1 was silenced. In contrast, the expression of LeHT1 was not inhibited in SlVRSLip-silenced R plants. Hence, in the hierarchy of the gene network conferring TYLCV resistance, SlVRSLip is downstream of LeHT1. Silencing of another gene involved in resistance, a Permease-I like protein, did not affect the expression of SlVRSLip and LeHT1; expression of the Permease was not affected by silencing SlVRSLip or LeHT1, suggesting that it does not belong to the same network. The triple co-silencing of SlVRSLip, LeHT1 and Permease provoked an immediate cessation of growth of R plants upon infection and the accumulation of large amounts of virus. SlVRSLip is the first lipocalin-like gene shown to be involved in resistance to a plant virus.


Assuntos
Begomovirus/patogenicidade , Resistência à Doença/genética , Regulação da Expressão Gênica de Plantas/genética , Lipocalinas/genética , Doenças das Plantas/imunologia , Solanum lycopersicum/genética , Animais , Sequência de Bases , Begomovirus/imunologia , Regulação da Expressão Gênica no Desenvolvimento/genética , Inativação Gênica , Genótipo , Hemípteros/virologia , Lipocalinas/metabolismo , Solanum lycopersicum/imunologia , Solanum lycopersicum/fisiologia , Solanum lycopersicum/virologia , Dados de Sequência Molecular , Proteínas de Transporte de Monossacarídeos/genética , Proteínas de Transporte de Monossacarídeos/metabolismo , Doenças das Plantas/virologia , Folhas de Planta/genética , Folhas de Planta/imunologia , Folhas de Planta/fisiologia , Folhas de Planta/virologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Caules de Planta/genética , Caules de Planta/imunologia , Caules de Planta/fisiologia , Caules de Planta/virologia , Espécies Reativas de Oxigênio/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Estresse Fisiológico
6.
Plants (Basel) ; 11(21)2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36365396

RESUMO

Tomato cultivation is threatened by environmental stresses (e.g., heat, drought) and by viral infection (mainly viruses belonging to the tomato yellow leaf curl virus family-TYLCVs). Unlike many RNA viruses, TYLCV infection does not induce a hypersensitive response and cell death in tomato plants. To ensure a successful infection, TYLCV preserves a suitable cellular environment where it can reproduce. Infected plants experience a mild stress, undergo adaptation and become partially "ready" to exposure to other environmental stresses. Plant wilting and cessation of growth caused by heat and drought is suppressed by TYLCV infection, mainly by down-regulating the heat shock transcription factors, HSFA1, HSFA2, HSFB1 and consequently, the expression of HSF-regulated stress genes. In particular, TYLCV captures HSFA2 by inducing protein complexes and aggregates, thus attenuating an acute stress response, which otherwise causes plant death. Viral infection mitigates the increase in stress-induced metabolites, such as carbohydrates and amino acids, and leads to their reallocation from shoots to roots. Under high temperatures and water deficit, TYLCV induces plant cellular homeostasis, promoting host survival. Thus, this virus-plant interaction is beneficial for both partners.

7.
Mol Plant Pathol ; 23(4): 475-488, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-34970822

RESUMO

With climate warming, drought becomes a vital challenge for agriculture. Extended drought periods affect plant-pathogen interactions. We demonstrate an interplay in tomato between drought and infection with tomato yellow leaf curl virus (TYLCV). Infected plants became more tolerant to drought, showing plant readiness to water scarcity by reducing metabolic activity in leaves and increasing it in roots. Reallocation of osmolytes, such as carbohydrates and amino acids, from shoots to roots suggested a role of roots in protecting infected tomatoes against drought. To avoid an acute response possibly lethal for the host organism, TYLCV down-regulated the drought-induced activation of stress response proteins and metabolites. Simultaneously, TYLCV promoted the stabilization of osmoprotectants' patterns and water balance parameters, resulting in the development of buffering conditions in infected plants subjected to prolonged stress. Drought-dependent decline of TYLCV amounts was correlated with HSFA1-controlled activation of autophagy, mostly in the roots. The tomato response to combined drought and TYLCV infection points to a mutual interaction between the plant host and its viral pathogen.


Assuntos
Begomovirus , Solanum lycopersicum , Begomovirus/fisiologia , Secas , Proteínas de Choque Térmico , Doenças das Plantas
8.
Cells ; 10(11)2021 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-34831098

RESUMO

A growing body of research points to a positive interplay between viruses and plants. Tomato yellow curl virus (TYLCV) is able to protect tomato host plants against extreme drought. To envisage the use of virus protective capacity in agriculture, TYLCV-resistant tomato lines have to be infected first with the virus before planting. Such virus-resistant tomato plants contain virus amounts that do not cause disease symptoms, growth inhibition, or yield loss, but are sufficient to modify the metabolism of the plant, resulting in improved tolerance to drought. This phenomenon is based on the TYLCV-dependent stabilization of amounts of key osmoprotectants induced by drought (soluble sugars, amino acids, and proteins). Although in infected TYLCV-susceptible tomatoes, stress markers also show an enhanced stability, in infected TYLCV-resistant plants, water balance and osmolyte homeostasis reach particularly high levels. These tomato plants survive long periods of time during water withholding. However, after recovery to normal irrigation, they produce fruits which are not exposed to drought, similarly to the control plants. Using these features, it might be possible to cultivate TYLCV-resistant plants during seasons characterized by water scarcity.


Assuntos
Adaptação Fisiológica , Begomovirus/fisiologia , Secas , Solanum lycopersicum/fisiologia , Solanum lycopersicum/virologia , Biomassa , Frutas/crescimento & desenvolvimento , Proteínas de Choque Térmico/metabolismo , Solanum lycopersicum/crescimento & desenvolvimento , Doenças das Plantas/virologia , Proteínas de Plantas/metabolismo , Estômatos de Plantas/fisiologia , Transpiração Vegetal/fisiologia , Estabilidade Proteica
9.
Mol Microbiol ; 74(4): 974-89, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19818014

RESUMO

Dysfunction of the Neurospora crassa nuclear Dbf2-related kinase COT1 leads to cessation of tip extension and massive induction of new sites of growth. To determine the role phosphorylation plays in COT1 function, we mutated COT1 residues corresponding to positions of highly conserved nuclear Dbf2-related phosphorylation sites. Analyses of the point-mutation cot-1 strains (mimicking non- and constitutively phosphorylated states) indicate the involvement of COT1 phosphorylation in the regulation of hyphal elongation and branching as well as asexual development by altering cell wall integrity and actin organization. Phosphorylation of COT1's activation segment (at Ser417) is required for proper in vitro kinase activity, but has only a limited effect on hyphal growth. In marked contrast, even though phosphorylation of the C-terminal hydrophobic motif (at Thr589) is crucial for all COT1 functions in vivo, the lack of Thr589 phosphorylation did not significantly affect in vitro COT1 kinase activity. Nevertheless, its regulatory role has been made evident by the significant increase observed in COT1 kinase activity when this residue was substituted in a manner mimicking constitutive phosphorylation. We conclude that COT1 regulates elongation and branching in an independent manner, which is determined by its phosphorylation state.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Proteínas Fúngicas/metabolismo , Neurospora crassa/citologia , Neurospora crassa/crescimento & desenvolvimento , Proteínas Quinases/metabolismo , Actinas/metabolismo , Substituição de Aminoácidos , Animais , Proteínas de Ciclo Celular/genética , Parede Celular/metabolismo , Proteínas Fúngicas/genética , Hifas/citologia , Hifas/crescimento & desenvolvimento , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Mutagênese Sítio-Dirigida , Fosforilação , Mutação Puntual , Proteínas Quinases/genética
10.
Planta ; 231(3): 537-48, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19946703

RESUMO

To identify genes involved in resistance of tomato to Tomato yellow leaf curl virus (TYLCV), cDNA libraries from lines resistant (R) and susceptible (S) to the virus were compared. The hexose transporter LeHT1 was found to be expressed preferentially in R tomato plants. The role of LeHT1 in the establishment of TYLCV resistance was studied in R plants where LeHT1 has been silenced using Tobacco rattle virus-induced gene silencing (TRV VIGS). Following TYLCV inoculation, LeHT1-silenced R plants showed inhibition of growth and enhanced virus accumulation and spread. In addition, a necrotic response was observed along the stem and petioles of infected LeHT1-silenced R plants, but not on infected not-silenced R plants. This response was specific of R plants since it was absent in infected LeHT1-silenced S plants. Necrosis had several characteristics of programmed cell death (PCD): DNA from necrotic tissues presented a PCD-characteristic ladder pattern, the amount of a JNK analogue increased, and production of reactive oxygen was identified by DAB staining. A similar necrotic reaction along stem and petioles was observed in LeHT1-silenced R plants infected with the DNA virus Bean dwarf mosaic virus and the RNA viruses Cucumber mosaic virus and Tobacco mosaic virus. These results constitute the first evidence for a necrotic response backing natural resistance to TYLCV in tomato, confirming that plant defense is organized in multiple layers. They demonstrate that the hexose transporter LeHT1 is essential for the expression of natural resistance against TYLCV and its expression correlates with inhibition of virus replication and movement.


Assuntos
Begomovirus/fisiologia , Proteínas de Membrana Transportadoras/genética , Doenças das Plantas/virologia , Proteínas de Plantas/genética , Solanum lycopersicum/virologia , Sequência de Bases , Inativação Gênica , Imunidade Inata/genética , Solanum lycopersicum/genética , Solanum lycopersicum/crescimento & desenvolvimento , Proteínas de Membrana Transportadoras/química , Proteínas de Membrana Transportadoras/metabolismo , Dados de Sequência Molecular , Necrose , Estresse Oxidativo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Análise de Sequência de DNA
11.
Sci Rep ; 10(1): 1856, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-32024917

RESUMO

Pharmaceuticals remain in treated wastewater used to irrigate agricultural crops. Their effect on terrestrial plants is practically unknown. Here we tested whether these compounds can be considered as plant stress inducers. Several features characterize the general stress response in plants: production of reactive oxygen species acting as stress-response signals, MAPKs signaling cascade inducing expression of defense genes, heat shock proteins preventing protein denaturation and degradation, and amino acids playing signaling roles and involved in osmoregulation. Tomato seedlings bathing in a cocktail of pharmaceuticals (Carbamazepine, Valporic acid, Phenytoin, Diazepam, Lamotrigine) or in Carbamazepine alone, at different concentrations and during different time-periods, were used to study the patterns of stress-related markers. The accumulation of the stress-related biomarkers in leaf and root tissues pointed to a cumulative stress response, mobilizing the cell protection machinery to avoid metabolic modifications and to restore homeostasis. The described approach is suitable for the investigation of stress response of different crop plants to various contaminants present in treated wastewater.


Assuntos
Preparações Farmacêuticas/administração & dosagem , Solanum lycopersicum/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Águas Residuárias/química , Produtos Agrícolas/efeitos dos fármacos , Solanum lycopersicum/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Plântula/efeitos dos fármacos , Plântula/metabolismo
12.
Genetics ; 179(3): 1313-25, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18562669

RESUMO

Ndr kinases, such as Neurospora crassa COT1, are important for cell differentiation and polar morphogenesis, yet their input signals as well as their integration into a cellular signaling context are still elusive. Here, we identify the cot-1 suppressor gul-4 as mak-2 and show that mutants of the gul-4/mak-2 mitogen-activated protein (MAP) kinase pathway suppress cot-1 phenotypes along with a concomitant reduction in protein kinase A (PKA) activity. Furthermore, mak-2 pathway defects are partially overcome in a cot-1 background and are associated with increased MAK1 MAPK signaling. A comparative characterization of N. crassa MAPKs revealed that they act as three distinct modules during vegetative growth and asexual development. In addition, common functions of MAK1 and MAK2 signaling during maintenance of cell-wall integrity distinguished the two ERK-type pathways from the p38-type OS2 osmosensing pathway. In contrast to separate functions during vegetative growth, the concerted activity of the three MAPK pathways is essential for cell fusion and for the subsequent formation of multicellular structures that are required for sexual development. Taken together, our data indicate a functional link between COT1 and MAPK signaling in regulating filamentous growth, hyphal fusion, and sexual development.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Núcleo Celular/enzimologia , Proteínas Fúngicas/metabolismo , Hifas/enzimologia , Neurospora crassa/enzimologia , Neurospora crassa/crescimento & desenvolvimento , Proteínas Quinases/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Deleção de Genes , Histidina Quinase , Hifas/citologia , Hifas/crescimento & desenvolvimento , Modelos Biológicos , Neurospora crassa/citologia , Fenótipo , Proteínas Serina-Treonina Quinases
13.
Arch Virol ; 154(3): 399-407, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19184338

RESUMO

Transgenesis offers many ways to obtain virus-resistant plants. However, in most cases resistance is against a single virus or viral strain. We have taken a novel approach based on the ability of a whitefly endosymbiotic GroEL to bind viruses belonging to several genera, in vivo and in vitro. We have expressed the GroEL gene in Nicotiana benthamiana plants, postulating that upon virus inoculation, GroEL will bind to virions, thereby interfering with pathogenesis. The transgenic plants were inoculated with the begomovirus tomato yellow leaf curl virus (TYLCV) and the cucumovirus cucumber mosaic virus (CMV), both of which interacted with GroEL in vitro, and with the trichovirus grapevine virus A (GVA) and the tobamovirus tobacco mosaic virus (TMV), which did not. While the transgenic plants inoculated with TYLCV and CMV presented a high level of tolerance, those inoculated with GVA and TMV were susceptible. The amounts of virus in tolerant transgenic plants was lower by three orders of magnitude than those in non-transgenic plants; in comparison, the amounts of virus in susceptible transgenic plants were similar to those in non-transgenic plants. Leaf extracts of the tolerant plants contained GroEL-virus complexes. Hence, tolerance was correlated with trapping of viruses in planta. This study demonstrated that multiple resistances to viruses belonging to several different taxonomic genera could be achieved. Moreover, it might be hypothesized that plants expressing GroEL will be tolerant to those viruses that bind to GroEL in vitro, such as members of the genera Begomovirus, Cucumovirus, Ilarvirus, Luteovirus, and Tospovirus.


Assuntos
Chaperonina 60/imunologia , Imunidade Inata , Proteínas de Insetos/imunologia , Doenças das Plantas/virologia , Vírus de Plantas/imunologia , Animais , Begomovirus/imunologia , Chaperonina 60/genética , Cucumovirus/imunologia , Flexiviridae/imunologia , Hemípteros/genética , Proteínas de Insetos/genética , Plantas Geneticamente Modificadas , Nicotiana/genética , Nicotiana/virologia , Vírus do Mosaico do Tabaco/imunologia
14.
Mol Biol Cell ; 17(9): 4080-92, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16822837

RESUMO

Members of the Ste20 and NDR protein kinase families are important for normal cell differentiation and morphogenesis in various organisms. We characterized POD6 (NCU02537.2), a novel member of the GCK family of Ste20 kinases that is essential for hyphal tip extension and coordinated branch formation in the filamentous fungus Neurospora crassa. pod-6 and the NDR kinase mutant cot-1 exhibit indistinguishable growth defects, characterized by cessation of cell elongation, hyperbranching, and altered cell-wall composition. We suggest that POD6 and COT1 act in the same genetic pathway, based on the fact that both pod-6 and cot-1 can be suppressed by 1) environmental stresses, 2) altering protein kinase A activity, and 3) common extragenic suppressors (ropy, as well as gul-1, which is characterized here as the ortholog of the budding and fission yeasts SSD1 and Sts5, respectively). Unlinked noncomplementation of cot-1/pod-6 alleles indicates a potential physical interaction between the two kinases, which is further supported by coimmunoprecipitation analyses, partial colocalization of both proteins in wild-type cells, and their common mislocalization in dynein/kinesin mutants. We conclude that POD6 acts together with COT1 and is essential for polar cell extension in a kinesin/dynein-dependent manner in N. crassa.


Assuntos
Polaridade Celular , Proteínas Fúngicas/metabolismo , Neurospora crassa/citologia , Neurospora crassa/enzimologia , Proteínas Serina-Treonina Quinases/metabolismo , Sequência de Aminoácidos , Domínio Catalítico , Polaridade Celular/efeitos dos fármacos , Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores , Quinases do Centro Germinativo , Hifas/citologia , Microtúbulos/metabolismo , Proteínas Motores Moleculares/metabolismo , Dados de Sequência Molecular , Mutação/efeitos dos fármacos , Mutação/genética , Neurospora crassa/efeitos dos fármacos , Fenótipo , Ligação Proteica/efeitos dos fármacos , Proteínas Serina-Treonina Quinases/química , Transporte Proteico/efeitos dos fármacos , Alinhamento de Sequência , Cloreto de Sódio/farmacologia
15.
Fungal Genet Biol ; 45(2): 103-16, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17625933

RESUMO

A defect in mcb, encoding the cAMP-dependent protein kinase A (PKA) regulatory subunit in Neurospora crassa, which confers an apolar growth phenotype, is accompanied by an increase in PKA activity levels. Both PKA and CRE-1 [a key carbon catabolite repression (CCR) regulator] mediate the cellular response to carbon-source availability. Inactivation of the cre-1 gene resulted in reduced growth rate, abnormal hyphal morphology and altered CCR. Both PKA and CRE-1 affected morphology in a carbon-dependent manner, as fructose suppressed the apolar morphology of the mcb strain and enabled faster growth of the Deltacre-1 mutant. An increase in cre-1 transcript abundance was observed in mcb and a reduction in PKA activity levels was measured in Deltacre-1. CRE-1 is involved in determining PKA-dependent polarity, as an mcb;Deltacre-1 strain displayed partial reestablishment of hyphal polarity. Taken together, our results demonstrate regulatory interactions between PKA and CRE-1 that affect cell polarity in a filamentous fungus.


Assuntos
Fator 2 Ativador da Transcrição/fisiologia , Proteínas Quinases Dependentes de AMP Cíclico/fisiologia , Proteínas Fúngicas/fisiologia , Regulação Fúngica da Expressão Gênica , Neurospora crassa/fisiologia , Polaridade Celular , Frutose , Genes Fúngicos , Hifas , Subunidades Proteicas/genética
16.
Plant Physiol Biochem ; 46(4): 482-92, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18171620

RESUMO

The defense response to several abiotic stresses has been compared in two tomato inbred lines issued from the same breeding program, one susceptible and the other resistant to Tomato yellow leaf curl virus (TYLCV) infection. The level of oxidative burst and the amounts of key regulatory stress proteins: pathogenesis-related proteins (PRs), heat shock proteins (HSPs) and mitogen-activated protein kinases (MAPKs) were appraised following treatments with NaCl, H(2)O(2), and ethanol. Significant differences in the response of the two tomato genotypes to these stresses have been found for HSPs and MAPKs patterns at the level of down-regulation but not activation. The higher abundance of HSPs and MAPKs in tomatoes resistant to TYLCV could result in enhanced defense capacity against abiotic stresses.


Assuntos
Regulação da Expressão Gênica de Plantas , Doenças das Plantas , Proteínas de Plantas/biossíntese , Vírus de Plantas , Transdução de Sinais , Solanum lycopersicum/metabolismo , Solanum lycopersicum/virologia , Estresse Oxidativo , Doenças das Plantas/virologia
17.
Oncotarget ; 9(44): 27487-27501, 2018 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-29938000

RESUMO

The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

18.
Mol Plant Microbe Interact ; 20(11): 1376-83, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17977149

RESUMO

To better understand the nature of resistance of tomato to the whitefly (Bemisia tabaci, B biotype)-transmitted Tomato yellow leaf curl virus (TYLCV), whiteflies and TYLCV were considered as particular cases of biotic stresses and virus resistance as a particular case of successful response to these stresses. Two inbred tomato lines issued from the same breeding program that used Solanum habrochaites as a TYLCV resistance source, one susceptible and the other resistant, were used to compare the expression of key proteins involved at different stages of the plant response with stresses: mitogen-activated protein kinases (MAPKs), cellular heat shock proteins (HSPs, proteases), and pathogenesis-related (PR) proteins. The two biotic stresses-non-viruliferous whitefly feeding and virus infection with viruliferous insects--led to a slow decline in abundance of MAPKs, HSPs, and chloroplast protease FtsH (but not chloroplast protease ClpC), and induced the activities of the PR proteins, beta-1,3-glucanase, and peroxidase. This decline was less pronounced in virus-resistant than in virus-susceptible lines. Contrary to whitefly infestation and virus infection, inoculation with the fungus Sclerotinia sclerotiorum induced a rapid accumulation of the stress proteins studied, followed by a decline; the virus-susceptible and -resistant tomato lines behaved similarly in response to the fungus.


Assuntos
Regulação da Expressão Gênica de Plantas , Hemípteros/fisiologia , Doenças das Plantas/virologia , Proteínas de Plantas/metabolismo , Vírus de Plantas/fisiologia , Solanum lycopersicum/metabolismo , Solanum lycopersicum/virologia , Animais , Quitinases/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Perfilação da Expressão Gênica , Predisposição Genética para Doença , Genótipo , Glucosidases/metabolismo , Proteínas de Choque Térmico/metabolismo , Hemípteros/virologia , Solanum lycopersicum/genética , Solanum lycopersicum/parasitologia , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Peroxidases/metabolismo , Proteínas de Plantas/genética
19.
Front Plant Sci ; 8: 355, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28360921

RESUMO

Tomato yellow leaf curl virus (TYLCV), a begomovirus, induces protein aggregation in infected tomatoes and in its whitefly vector Bemisia tabaci. The interactions between TYLCV and HSP70 and HSP90 in plants and vectors are necessity for virus infection to proceed. In infected host cells, HSP70 and HSP90 are redistributed from a soluble to an aggregated state. These aggregates contain, together with viral DNA/proteins and virions, HSPs and components of the protein quality control system such as ubiquitin, 26S proteasome subunits, and the autophagy protein ATG8. TYLCV CP can form complexes with HSPs in tomato and whitefly. Nonetheless, HSP70 and HSP90 play different roles in the viral cell cycle in the plant host. In the infected host cell, HSP70, but not HSP90, participates in the translocation of CP from the cytoplasm into the nucleus. Viral amounts decrease when HSP70 is inhibited, but increase when HSP90 is downregulated. In the whitefly vector, HSP70 impairs the circulative transmission of TYLCV; its inhibition increases transmission. Hence, the efficiency of virus acquisition by whiteflies depends on the functionality of both plant chaperones and their cross-talk with other protein mechanisms controlling virus-induced aggregation.

20.
Cell Stress Chaperones ; 22(3): 345-355, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28324352

RESUMO

Tomato yellow leaf curl virus (TYLCV) is a begomovirus infecting tomato plants worldwide. TYLCV needs a healthy host environment to ensure a successful infection cycle for long periods. Hence, TYLCV restrains its destructive effect and induces neither a hypersensitive response nor cell death in infected tomatoes. On the contrary, TYLCV counteracts cell death induced by other factors, such as inactivation of HSP90 functionality. Suppression of plant death is associated with the inhibition of the ubiquitin 26S proteasome degradation and with a deactivation of the heat shock transcription factor HSFA2 pathways (including decreased HSP17 levels). The goal of the current study was to find if the individual TYLCV genes were capable of suppressing HSP90-dependent death and HSFA2 deactivation. The expression of C2 (C3 and CP to a lesser extent) caused a decrease in the severity of death phenotypes, while the expression of V2 (C1 and C4 to a lesser extent) strengthened cell death. However, C2 or V2 markedly affected stress response under conditions of viral infection. The downregulation of HSFA2 signaling, initiated by the expression of C1 and V2, was detected in the absence of virus infection, but was enhanced in infected plants, while CP and C4 mitigated HSFA2 levels only in the infected tomatoes. The dependence of analyzed plant stress response suppression on the interaction of the expressed genes with the environment created by the whole virus infection was more pronounced than on the expression of individual TYLCV genes.


Assuntos
Begomovirus/genética , Proteínas de Plantas/metabolismo , Solanum lycopersicum/metabolismo , Proteínas Virais/metabolismo , 3,3'-Diaminobenzidina/química , Begomovirus/metabolismo , Inativação Gênica , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Fatores de Transcrição de Choque Térmico/genética , Fatores de Transcrição de Choque Térmico/metabolismo , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/química , Solanum lycopersicum/virologia , Fotografação , Doenças das Plantas/virologia , Folhas de Planta/metabolismo , Folhas de Planta/virologia , Proteínas de Plantas/genética , Plasmídeos/genética , Plasmídeos/metabolismo , Estresse Fisiológico , Temperatura , Proteínas Virais/genética
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