RESUMO
We describe the recovery of the rare species Aeromonas culicicola, so far known only in mosquitoes in India, from a drinking water supply in Spain. Typing, using enterobacterial repetitive intergenic consensus-PCR, revealed that the 27 new isolates belonged to 3 very closely related strains. These strains were genetically identified by 16S rRNA gene sequencing. Spanish strains differed from the mosquito strains in three nucleotide positions. The AHCYTOEN gene was present in these water strains, which may have a public health significance.
Assuntos
Aeromonas/classificação , Aeromonas/isolamento & purificação , Culicidae/microbiologia , Ingestão de Líquidos , Abastecimento de Água , Aeromonas/genética , Animais , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , Enterotoxinas/genética , Água Doce/microbiologia , Genes de RNAr , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , EspanhaRESUMO
The APH(3')-II aminoglycoside-phosphotransferase has been studied by protein blotting. This technique allowed the detection of the enzyme in the crude extracts from clinical isolates of Enterobacteriaceae, and the molecular weight determination of the APH(3')-II. The obtained molecular weight of 25,000 is coincident with the previously reported value determined by a different method. These results demonstrate that this technique is useful for the study of aminoglycoside-modifying enzymes.
Assuntos
Fosfotransferases/análise , Western Blotting , Escherichia coli/enzimologia , Canamicina Quinase , Peso Molecular , Salmonella enteritidis/enzimologiaRESUMO
Saragossa city supply channel and the river Ebro (up and downstream urban sewage) were studied for the presence of coliphages and B. fragilis phages and their relationship with the bacterian faecal indicators. In the supply channel the coliphages geometric mean was of 130 ufp/100 ml, and showed no correlation with faecal and total coliforms, but it showed indirect correlation with ambient temperature. In the river Ebro the coliphages geometric mean ranged from 290 to 8,000 ufp/100 ml; the relationship with total and faecal coliforms and faecal streptococci was high, but they were temperature independent. With the methodology utilized B. fragilis phages only were recovered in samples with faecal coliforms levels > 1 x 10(4) ufc/100 ml.