Chondrogeneic Potential of MSC from Different Sources in Spheroid Culture.

We performed a comparative study of the proliferative potential of human mesenchymal stromal cells (MSC) from three sources (tooth pulp, adipose tissue, and Wharton's jelly) in spheroid culture; human chondroblasts served as the positive control. Histological examination revealed signs of chondrogenic differentiation in all studied cell cultures and the differences in the volume and composition of the extracellular matrix. Spheroids formed by MSC from the tooth pulp and Wharton's jelly were characterized by low content of extracellular matrix and glycosaminoglycans. Spheroids from adipose tissue MSC contained maximum amount of the extracellular matrix and high content of glycosaminoglycans. Chondrocytes produced glycosaminoglycan-enriched matrix. Type II collagen was produced by chondrocytes (to a greater extent) and adipose tissue MSC (to a lesser extent). The results of our study demonstrate that MSC from the adipose tissue under conditions of spheroid culturing exhibited maximum chondrogenic potential.

Formation of Tissue-Engineered Construct of Human Cartilage Tissue in a Flow-Through Bioreactor.

We performed culturing of a cell-engineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, human adipose tissue mesenchymal stromal cells, and induction chondrogenic culture medium in a specially designed flow-through bioreactor. On day 16 of the experiment, human adipose tissue mesenchymal stromal cells acquired flattened shape typical for chondroblasts, demonstrated high proliferative activity, and formed extracellular matrix. The observed histological changes in the cultured system attested to the beginning of the formation of a tissue-engineered construct of human cartilage tissue.

[The dynamics of C-reactive protein in the process of coronary artery bypass grafting in patients with ischemic heart disease].

The article presents the assessment of prognostic value of perioperative level of C-reactive protein in the development of post-operative cardio-vascular complications in patients with ischemic heart disease and underwent coronary artery bypass grafting. The sample consisted of 99 patients with stable forms of ischemic heart disease and underwent the planned coronary artery bypass grafting. The average age of patients was 58 +/- 4.19 years. All patients were applied direct revascularization of myocardium in conditions of artificial blood circulation. The patients were divided on the groups depending on occurrence of post-operational cardio-vascular complications. The group I consisted of 42 patients with non-complicated post-operational period (42%). The group 2 consisted of 57 patients with cardio-vascular complications and included the subgroup of 24 patients with fibrillation of atriums developed in post-operational period. The concentration of C-reactive protein was analyzed in blood serum one day before the operation and at first and seventh days after operation. The post-operative period after application of coronary artery bypass in conditions of ischemic heart disease is characterized by activation of inflammation reaction evaluated by concentration of C-reactive protein. The pre-operation level of C-reactive protein can be used as prognostic marker of development of cardio-vascular complications, post-operative fibrillation of atriums after application of coronary artery bypass in particular

[Comparative pharmacokinetics of dihydroquercetin in rats upon peroral administration of a parent compound and liposomal flamen D].

The pharmacokinetics of dihydroquercetin (DHQ) in the forms of parent substance and a new liposomal formulation (Flamena D) have been studied in rats upon single peroral administration in a dose of 50 mg/kg. DHQ concentration after enzymatic hydrolysis in the blood plasma was determined by HPLC with UV detection. The elimination half-life of DHQ introduced in the form of Flamena D was about T(1/2) = 1.3 h. The relative bioavailability of DHQ after the administration of Flamena D amounted to 159% in comparison to that of the parent substance of DHQ.

[Detection of bensultap (bancol) derivatives and metabolites by chromatographic methods].

This paper is designed to describe methods for the detection of derivatives and metabolites of the insecticidic agent bancol (nereistoxin precursor) by chromatographic methods including thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), gas chromatography with a nitrogen-phosphorous detector (GC-NPD), and gas chromatography mass spectrometry (GH-MS). The data obtained confirm the high diagnostic value of assays for derivatives and metabolites compared with the determination of the respective parent insecticides. New variants of extraction of these compounds from urine and various tissues by liquid-liquid and solid-phase chromatography are proposed. Their spectral and chromatographic characteristics are reported.

[Biorhythmical characterization of exacerbations of the degenerative-dystrophic process in patients with the impaired hip joint]. / Bioritmicheskaia kharakteristika obostrenii degenerativno-distroficheskogo protsessa u patsientov s porazheniem tazobedrennogo sustava.

The age-dependent structure of the contingents treated conservatively at the local outpatient clinics (197 women aged 17-82 and 59 men aged 25-85) and patients subjected to operations of total endoprosthesis of the hip joint for coxarthrosis (663 women aged 19-89 and 304 men aged 22-82) was investigated. The structure of the indices under study was found to fluctuate with the average period length 10.7 years (sigma = 2.7 years), 8.2 years (sigma = 3.5 years), 7.3 days (sigma = 3.0 years), 9.5 years (sigma = 2.1 years) respectively. A comparison of the fluctuation curves of the age-dependent structure of the women treated conservatively and operated upon showed the coincidence of the maximums in 35, 43, 60, 65, 69, 74-74 years of age, in men--in 34-35, 41, 75-76. It can be supposed that the revealed age-dependent fluctuations of activity of the inflammatory process and the pain syndrome correspondingly might be considered as an element of the periodic disease in pathogenesis of degenerative-dystrophic lesions of the hip joint.