MicroRNA-155 and its exosomal form: Small pieces in the gastrointestinal cancers puzzle.

Gastrointestinal (GI) cancers are common cancers that are responsible for a large portion of global cancer fatalities. Due to this, there is a pressing need for innovative strategies to identify and treat GI cancers. MicroRNAs (miRNAs) are short ncRNAs that can be considered either cancer-causing or tumor-inhibiting molecules. MicroRNA-155, also known as miR-155, is a vital regulator in various cancer types. This miRNA has a carcinogenic role in a variety of gastrointestinal cancers, including pancreatic, colon, and gastric cancers. Since the abnormal production of miR-155 has been detected in various malignancies and has a correlation with increased mortality, it is a promising target for future therapeutic approaches. Moreover, exosomal miR-155 associated with tumors have significant functions in communicating between cells and establishing the microenvironment for cancer in GI cancers. Various types of genetic material, such as specifically miR-155 as well as proteins found in cancer-related exosomes, have the ability to be transmitted to other cells and have a function in the advancement of tumor. Therefore, it is critical to conduct a review that outlines the diverse functions of miR-155 in gastrointestinal malignancies. As a result, we present a current overview of the role of miR-155 in gastrointestinal cancers. Our research highlighted the role of miR-155 in GI cancers and covered critical issues in GI cancer such as pharmacologic inhibitors of miRNA-155, miRNA-155-assosiated circular RNAs, immune-related cells contain miRNA-155. Importantly, we discussed miRNA-155 in GI cancer resistance to chemotherapy, diagnosis and clinical trials. Furthermore, the function of miR-155 enclosed in exosomes that are released by cancer cells or tumor-associated macrophages is also covered.

A Halogen-Bonded Fluorescent Molecular Photoswitch: Transition from 3D Cubic Lattice to 1D Helical Superstructure for Polarization Inversion of Circularly Polarized Luminescence.

The fabrication of materials that can switch between circularly polarized luminescence (CPL) signals is both essential and challenging. Here, two new halogen-bonded fluorescent molecular photoswitches, namely, HB-switch 1 and HB-switch 2, containing α-cyano-substituted diarylethene compounds with different end groups were developed. Upon exposure to specific UV or visible light wavelengths, they exhibited controllable and reversible Z/E photoisomerization. When these switches were integrated into blue-phase liquid crystals (BPLCs), the temperature range of BP significantly expanded. Notably, the BP system incorporating HB-switch 1 exclusively achieved reversible polarization inversion of CPL signals under irradiation with specific UV/Visible light and during cooling/heating. The photo/thermal dual-response behavior of the CPL signals can be attributed to the phase transition from a high-symmetry 3D BP Icubic lattice to a low-symmetry 1D helical superstructure induced by the Z/E photoisomerization of HB-switch 1 and temperature changes. This study underscores the significance of employing halogen-bond assembly strategies to design materials with switchable CPL signals, opening new possibilities for CPL-active systems.

Prognostic value of circulating tumor DNA in operable non-small cell lung cancer: a systematic review and reconstructed individual patient-data based meta-analysis.

BACKGROUND: This reconstructed individual patient data (IPD)-based meta-analysis is aimed to summarize the current findings and comprehensively investigate the predictive value of circulating tumor DNA (ctDNA) in operable non-small cell lung cancer (NSCLC). METHODS: PubMed, Cochrane and Embase were searched to include potentially eligible studies. The primary outcomes included progression-free survival (DFS) by ctDNA status at baseline, postoperative, and longitudinal timepoints. The IPD-based survival data was retracted and used in reconstructed IPD-based meta-analysis. Subgroup analysis was implemented based on the baseline characteristics. RESULTS: Totally, 28 studies were involved, including 15 full-length articles (1686 patients) for IPD-based synthesis and 20 studies for conventional meta-analysis. The IPD-based meta-analysis discovered that patients with positive ctDNA status at the baseline (hazard ratio, HR = 3.73, 95% confidential interval, CI: 2.95-4.72), postoperative (3.96, 2.19-7.16), or longitudinal timepoints (12.33, 8.72-17.43) showed significantly higher risk of recurrence. Patients with persistent ctDNA-negative status had the lowest recurrence rate, and the negative conversion of ctDNA from baseline to postoperative timepoints was correlated with elevated DFS. Subgroup analyses suggested that stage II-III patients with ctDNA-positive status may achieve preferable therapeutic outcomes. CONCLUSIONS: Plasm ctDNA monitoring shows excellent clinical significance at the tested timepoints. Perioperative conversion of ctDNA status may indicate the therapeutic effect of radical surgery. Postoperative adjuvant therapy may be determined according to the ctDNA status. TRAIL REGISTRATION: CRD42022304445.

Polymer-Stabilized Liquid Crystal Films Containing Dithienyldicyanoethene-Based Chiral Photoswitch: Multi-Modulation for Environment-Adaptative Smart Windows.

A polymer-stabilized liquid crystal (PSLC)-based environment-adaptative smart window with multi-modulations is demonstrated. This PSLC system contains a right-handed dithienyldicyanoethene-based chiral photoswitch and a chiral dopant, S811, with opposite handedness, of which the reversible cis-trans photoisomerization of the switch can drive self-shading of the smart window under UV light stimulus because of the transition from nematic phase to cholesteric one. With the assistance of solar heat, the opacity of the smart window can be deepened because the heat promotes the isomerization conversion rate of the switch. This switch has no thermal relaxation at room temperature, therefore, the smart window exhibits dual stabilization: transparent state (cis-isomer) and opaque state (trans-isomer). Moreover, the incident intensity of sunlight can be regulated by an electric field, which allows the smart window to adapt to some specific situations. Such an energy-saving device can be used in buildings and vehicles to control indoor temperature and adapt to the required ambiance.

CCL3-CCR5 axis promotes cell migration and invasion of colon adenocarcinoma via Akt signaling pathway.

BACKGROUND: Infiltration of tumor-associated macrophages (TAMs) can promote tumorigenesis and development. C-C motif chemokine ligand 3 (CCL3) was reported to be derived from TAMs and tumor cells and facilitate the progression of several cancers. Nevertheless, whether CCL3 can be derived from TAMs and tumor cells of colon adenocarcinoma (COAD) is unclarified. METHODS: Peripheral blood monocytes-derived macrophages were polarized by the conditioned medium from COAD cells to establish TAM-like macrophages (TAM1/2). RT-qPCR and western blotting were used for detection of expression levels of CCL3 and its receptors C-C motif chemokine receptor 1 (CCR1) and CCR5 in TAM1/2 and COAD cells. Immunofluorescence staining was utilized for evaluating CCL3, CD163 and CCR5 expression. The Akt signaling pathway-associated protein levels were measured by western blotting. Transwell assays were used for assessing cell migration and invasiveness. RESULTS: CCL3 displayed a high level in TAMs and cancer cells of COAD. CCL3 activated the Akt signaling pathway by binding to CCR5. CCL3-CCR5 axis facilitated COAD cell migration and invasiveness by activating the Akt signaling. CCL3 derived from both TAMs and cancer cells contributed to the malignant behaviors of COAD cells. High expression of CCL3/CCR5 was closely associated with poor prognoses of COAD patients. CONCLUSION: CCL3-CCR5 interaction promotes cell migration and invasiveness, and functions as a prognostic biomarker for COAD.

A Photo- and Thermo-Driven Azoarene-Based Circularly Polarized Luminescence Molecular Switch in a Liquid Crystal Host.

The development of chiral optical active materials with switchable circularly polarized luminescence (CPL) signals remains a challenge. Here an azoarene-based circularly polarized luminescence molecular switch, (S, R, S)-switch 1 and (R, R, R)-switch 2, are designed and prepared with an (R)-binaphthyl azo group as a chiral photosensitive moiety and two (S)- or (R)-binaphthyl fluorescent molecules with opposite or the same handedness as chiral fluorescent moieties. Both switches exhibit reversible trans/cis isomerization when irradiated by 365 nm UV light and 520 nm green light in solvent and liquid crystal (LC) media. In contrast with the control (R, R, R)-switch 2, when switch 1 is doped into nematic LCs, polarization inversion and switching-off of the CPL signals are achieved in the resultant helical superstructure upon irradiation with 365 nm UV and 520 nm green light, respectively. Meanwhile, the fluorescence intensity of the system is basically unchanged during this switching process. In particular, these variations of the CPL signals could be recovered after heating, realizing the true sense of CPL reversible switching. Taking advantage of the unique CPL switching, the proof-of-concept for "a dual-optical information encryption system" based on the above CPL active material is demonstrated.

Near-infrared light-induced photoisomerization and photodissociation of a chiral fluorescent photoswitch in cholesteric liquid crystals assisted by upconversion nanoparticles.

Upconversion-luminescence-induced reflective color switching and fluorescence tuning of a cholesteric liquid crystal (CLC) cells were investigated. The CLC system was constructed by co-doping a chiral fluorescence photoswitch, switch 5, and upconversion nanoparticles (UCNPs) into nematic LC media. Under irradiation with 980 nm NIR light, the UCNPs emit both 450 nm blue light and 365 nm UV light to induce the simultaneous Z-to-E and E-to-Z photoisomerization of switch 5. This continuous rotation-inversion movement further leads to an irreversible photoisomerization and photodissociation of dicyanodistyrylthiophene moieties in switch 5. As a result, the reflective color of the CLC cell changed from blue to red and the fluorescence intensity decreased as well when exposed to 980 nm NIR light. Finally, optically written reflective-photoluminescent dual mode CLC cells were further demonstrated.

RNA-binding protein RBM38 inhibits colorectal cancer progression by partly and competitively binding to PTEN 3'UTR with miR-92a-3p.

RNA-binding motif protein 38 (RBM38) belongs to the RNA recognition motif family of RNA-binding proteins (RBPs). RBM38 was previously identified to suppress tumorigenesis in colorectal cancer (CRC). RBM38 was also reported to bind to the 3'UTR of phosphatase and tensin homolog gene on chromosome 10 (PTEN), a tumor suppressor involved in many cellular processes, to stabilize PTEN transcripts. In the present study, we investigated the mechanisms underlying the regulation of RBM38 in CRC. Reverse transcription quantitative polymerase chain reaction and western blotting detected the expression of RBM38, PTEN, and miR-92a-3p. Colony formation, EdU, sphere formation, Transwell invasion, and in vivo assays examined the influence of RBM38 on CRC progression. Furthermore, RNA immunoprecipitation (RIP) assay determined the binding site of RBM38 on PTEN 3'UTR. The binding of miR-92a-3p or RBM38 on PTEN 3'UTR was assessed by luciferase reporter and RIP assays. We discovered that RBM38 was downregulated in CRC cells and tissues. RBM38 repressed CRC progression in vitro and in vivo. Furthermore, RBM38 upregulated and stabilized PTEN expression. Interestingly, the overexpression of PTEN reversely attenuated the promotion of RBM38 depletion on CRC progression. Additionally, RBM38 competed with miR-92a-3p in binding to PTEN 3'UTR. In conclusion, RBM38 inhibits CRC progression by competitively binding to PTEN 3'UTR with miR-92a-3p.

Irradiation-Wavelength Directing Circularly Polarized Luminescence in Self-Organized Helical Superstructures Enabled by Hydrogen-Bonded Chiral Fluorescent Molecular Switches.

Two light-driven chiral fluorescent molecular switches, (R,S,R)-switch 1 and (R,S,R)-switch 2, are prepared by means of hydrogen-bonded (H-bonded) assembly of a photoresponsive (S) chiral fluorescent molecule, respectively with a cyano substitution at different positions as an H-bond acceptor and an opposite (R) chiral molecule as an H-bond donor. The resulting two switches exhibit tunable and reversible Z/E photoisomerization irradiated with 450 nm blue and 365 nm UV light. When doped into an achiral liquid crystal, both switches are found to be able to form a CPL tunable luminescent helical superstructure. In contrast to the tunable CPL characteristics of the system incorporating switch 2, exposure of the system incorporating switch 1 to 365 nm and 450 nm radiation can lead to controllable different photostationary CPL behavior, including switching-off and polarization inversion. In addition, optical information coding is demonstrated using the system containing switch 1.

1,2-Dithienyldicyanoethene-Based, Visible-Light-Driven, Chiral Fluorescent Molecular Switch: Rewritable Multimodal Photonic Devices.

Reported here is the first example of a 1,2-dithienyldicyanoethene-based visible-light-driven chiral fluorescent molecular switch that exhibits reversible trans to cis photoisomerization. The trans form in solution almost completely transforms into the cis form, accompanied by a 10-fold decrease in its fluorescence intensity within 60 seconds when exposed to green light (520 nm). The reverse isomerization proceeds upon irradiation with blue light (405 nm). When doped into commercially available achiral liquid crystal hosts, this molecular switch efficiently induces luminescent helical superstructures, that is, a cholesteric phase. The intensity of the circularly polarized fluorescence as well as the selective reflection wavelength of the induced cholesteric phases can be reversibly tuned using visible light of two different wavelengths. Optically rewritable photonic devices using cholesteric films containing this molecular switch are described.

Dual-Responsive SPMA-Modified Polymer Photonic Crystals and Their Dynamic Display Patterns.

Light and electrothermal responsive polymer photonic crystals (PCs) modified with 1'-acryloyl chloride-3',3'-dimethyl-6-nitro-spiro(2H-1-benzopyran-2,2'-indoline) (SPMA) are proposed, and their dynamic display patterns are achieved through the combination of the SPMA-modified PCs and a patterned graphite layer. These PCs exhibit fluorescence under UV light irradiation because of the isomerization of the SPMA, which is restricted in the shell of the polymer colloidal spheres. After a voltage is applied to the patterned graphite layer, the fluorescence of PCs in the specific area disappears, and dynamic display patterns are obtained. Under UV light irradiation, the PCs change from the "partial-fluorescence" state to the initial "fluorescence" state, and the patterns disappear. Using this technique, the PC pattern "M L N" on the glass substrate and PC patterns from "0" to "9" on the paper substrate are fabricated. Thus, these dual-responsive PCs have potential applications in information recording, anticounterfeiting, dynamic display, and photoelectric devices.

Thermo-responsive shape and optical memories of photonic composite films enabled by glassy liquid crystalline polymer networks.

We propose a novel shape and optical memories of a photonic composite film based on a silica opal photonic crystal (PC) template and a liquid crystal polymer network (LCN). Here, the photonic composite film was fabricated by introducing a LCN precursor into a silica opal PC template, followed by UV photo-polymerization and then by the removal of the template. The obtained bilayer-structure photonic film was found to spontaneously form a three-dimensional (3D) temporary bending shape in response to heating, and thus the corresponding reflection color of the photonic composite film shows a blue shift during bending deformation. The inherent mechanisms of these two observations could be attributed to the variations of the LC molecule orientation and the light reflection in the photonic composite film during the thermal process. More intriguingly, the resulting temporary bending shape was fixed by applying mechanical force during slowly cooling down to the room temperature or autonomously fixed by a rapid cooling in liquid nitrogen. Additionally, this temporary state could restore back to the permanent flat shape when the film is cooled from the heat source without an external force. Finally, more complex 3D shape-memory samples could also be achieved by simply controlling the LC alignment or designing the sample geometry. This work opens up a new way to develop a novel shape-memory polymer photonic film.

Effects of terminal chain length in hydrogen-bonded chiral switches on phototunable behavior of chiral nematic liquid crystals: helicity inversion and phase transition.

A novel series of photoresponsive chiral switches are fabricated by a facile hydrogen-bonded (H-bonded) assembly method, in which the binaphthyl azobenzene molecule is used as the proton acceptor, and binaphthyl acids with opposite chiral configuration are proton donors. We find that the helical twisted power of H-bonded chiral switches and the helical handedness of induced chiral nematic liquid crystals (N*-LCs) are mainly determined by the terminal flexible chain length in proton donors of binaphthyl acids. Controlling the lengths of the terminal flexible chain leads to different photoswitching behaviors by light irradiation, such as a helical inversion in the N*-LCs and a phase transition from N*-LCs to nematic LCs. This is mainly because of chiral counteraction and intensity attenuation of opposite chiral configurations between the proton acceptor and proton donor during UV-vis irradiation. Additionally, the thermal switching behavior of N*-LCs doped with H-bonded chiral switches is also demonstrated, and the related tuning mechanism may be attributed to the H-bonded effect and the changes in a dihedral angle of the binaphthyl rings. This facile assembly approach provides a new way for the fabrication of functional chiral switches for photonic applications.

High expression of CASK correlates with progression and poor prognosis of colorectal cancer.

Calcium/calmodulin-dependent serine protein kinase (CASK), which localizes at cell-cell adhesion sites and binds to the heparan sulfate proteoglycan syndecan-2, is involved in cell proliferation, cytoskeletal remodeling, and cell migration. To demonstrate the role of CASK in colorectal cancer (CRC) carcinogenesis, we examined the expression of CASK and its binding protein syndecan-2 in human CRC tissues. The expression of CASK was measured in CRC specimens and the controls from adenomas and normal mucosae by immunohistochemical staining and Western blot analysis. Syndecan-2 protein level was tested in CRC samples and the controls by Western blot analysis. The correlations between CASK expression and clinicopathological variables, including disease-free survival (DFS) and overall survival (OS), were analyzed. Compared to the controls, both CASK and syndecan-2 expression were enhanced in CRC tissues. Furthermore, high expression of CASK and syndecan-2 was significantly correlated with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC. Survival analysis showed that patients with low CASK staining had a significantly better survival compared to patients with high CASK staining. In multivariate analysis, CASK overexpression, advanced tumor stage, lymph node metastasis, vasvular invasion, and liver metastasis were independent prognostic factors of poor DFS and OS. Our present study indicates that CASK overexpression is associated with an unfavorable prognosis. CASK is an independent prognostic factor for CRC, which suggests that it is a novel and crucial predictor for CRC metastasis.

Peroxiredoxin 2 is upregulated in colorectal cancer and contributes to colorectal cancer cells' survival by protecting cells from oxidative stress.

Peroxiredoxin 2 (Prdx2) is a member of the peroxiredoxin family, which is responsible for neutralizing reactive oxygen species. Prdx2 has been found to be elevated in several human cancer cells and tissues, including colorectal cancer (CRC), and it influences diverse cellular processes involving cells' survival, proliferation, and apoptosis, which suggests a possible role for Prdx2 in the maintenance of cancer cell. However, the mechanism by which Prdx2 modulates CRC cells' survival is unknown. The current study aimed to determine the effect of elevated Prdx2 on CRC cells and to further understand the underlying mechanisms. The results of this study showed that Prdx2 was upregulated in CRC tissues compared with the matched noncancer colorectal mucosa tissues and that Prdx2 expression was positively associated with tumor metastasis and the TNM stage. In the LoVo CRC cell line, Prdx2 was upregulated at both the RNA and protein levels compared with the normal FHC colorectal mucosa cell line. In addition, the LoVo CRC cell line was significantly more resistant to hydrogen peroxide (H2O2)-induced apoptosis because of a failure to activate pro-apoptotic pathways in contrast to Prdx2 knockdown cells. Suppression of Prdx2 using a lentiviral vector-mediated Prdx2-specific shRNA in the LoVo cell line restored H2O2 sensitivity. Our results suggested that Prdx2 has an essential role in regulating oxidation-induced apoptosis in CRC cells. Prdx2 may have potential as a therapeutic target in CRC.

Cluster-Triggered Self-Luminescence, Rapid Self-Healing, and Adaptive Reprogramming Liquid Crystal Elastomers Enabled by Dynamic Imine Bond.

The integration of advanced functions and diverse practical applications calls for multifunctional liquid crystal elastomers (LCEs); however, the structure-intrinsic luminescence and excellent mechanical properties of LCEs have not yet been explored. In this study, clusteroluminescence (CL)-based LCEs (CL-LCEs) are successfully fabricated without depending on large conjugated structures, thereby avoiding redundant organic synthesis and aggregation-caused quenching. The experimental and theoretical results reveal that secondary amine (-NH-) and imine (-C = N-) groups play vital roles in determining the presence of fluorescence in CL-LCEs. Based on the above observation, the strategy universalization and a molecular library for constructing CL-LCEs are further demonstrated. Meanwhile, the dynamic bond of imine bonds endows the CL-LCE system with rapid self-healing under mild conditions (70 °C in 10 min), excellent stretchability, and adaptive programmable characteristics. Furthermore, the self-luminescent performance enables visual detection of the self-healing process. Finally, CL-based information storage and anticounterfeiting are successfully realized and their applications in fiber actuators and fluorescent textiles are demonstrated. The distinctive luminescence and dynamic chemistry presented in this work has significant implications in elucidating the mechanism of CL and providing new strategies for the rational design of novel multifunctional LCE materials.

Recent Advances in Smart Windows Based on Photo-Responsive Liquid Crystals Featuring Phase Transition.

A smart window is an optical dimming device with intelligent functions that can control its relevant performances through external stimuli, achieving functions such as privacy protection and temperature regulation. Light is an ideal stimulus for regulating smart windows, which is noninvasive and allows self-adaptable manipulation of materials. This review highlights recent significant achievements in smart windows constructed by photo-responsive liquid crystals (LCs) systems that can undergo the transition between different phases. The smart windows based on photo-responsive LCs are used in a plethora of areas, including privacy protection, absorption glass, building decoration, energy saving, and climate modulation applications. The review concludes with a brief perspective on some significant challenges and opportunities for the future development of photo-responsive smart windows, which is crucial for expanding the applications of smart windows and improving their performances.

When quantum dots meet blue phase liquid crystal elastomers: visualized full-color and mechanically-switchable circularly polarized luminescence.

Polymer-based circularly polarized luminescence (CPL) materials with the advantage of diversified structure, easy fabrication, high thermal stability, and tunable properties have garnered considerable attention. However, adequate and precise tuning over CPL in polymer-based materials remains challenging due to the difficulty in regulating chiral structures. Herein, visualized full-color CPL is achieved by doping red, green, and blue quantum dots (QDs) into reconfigurable blue phase liquid crystal elastomers (BPLCEs). In contrast to the CPL signal observed in cholesteric liquid crystal elastomers (CLCEs), the chiral 3D cubic superstructure of BPLCEs induces an opposite CPL signal. Notably, this effect is entirely independent of photonic bandgaps (PBGs) and results in a high glum value, even without matching between PBGs and the emission bands of QDs. Meanwhile, the lattice structure of the BPLCEs can be reversibly switched via mechanical stretching force, inducing on-off switching of the CPL signals, and these variations can be further fixed using dynamic disulfide bonds in the BPLCEs. Moreover, the smart polymer-based CPL systems using the BPLCEs for anti-counterfeiting and information encryption have been demonstrated, suggesting the great potential of the BPLCEs-based CPL active materials.

Keratin gene signature expression drives epithelial-mesenchymal transition through enhanced TGF-ß signaling pathway activation and correlates with adverse prognosis in lung adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) stands as the foremost histological subtype of non-small-cell lung cancer, accounting for approximately 40% of all lung cancer diagnoses. However, there remains a critical unmet need to enhance the prediction of clinical outcomes and therapy responses in LUAD patients. Keratins (KRTs), serving as the structural components of the intermediate filament cytoskeleton in epithelial cells, play a crucial role in the advancement of tumor progression. This study investigated the prognostic significance of the KRT family gene and developed a KRT gene signature (KGS) for prognostic assessment and treatment guidance in LUAD. Methods: Transcriptome profiles and associated clinical details of LUAD patients were meticulously gathered from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The KGS score was developed based on the expression of five prognostic KRT genes (KRT7, KRT8, KRT17, KRT18, and KRT80), and the upper quartile of the KGS score was chosen as the cutoff. The Kaplan-Meier method was evaluated to compare survival outcomes between KGS-high and KGS-low groups. The underlying mechanism was further investigated by GSEA, GSVA, and other bioinformatic algorithms. Results: High expression of the KGS signature exhibited a robust association with poorer overall survival (OS) in the TCGA-LUAD dataset (HR: 1.81; 95% CI: 1.35-2.42, P = 0.00011). The association was further corroborated in three external GEO cohorts, including GSE31210 (HR: 3.31; 95% CI: 1.7-6.47, P = 0.00017), GSE72094 (HR: 1.95; 95% CI: 1.34-2.85, P = 0.00057) and GSE26939 (HR: 3.19; 95% CI: 1.74-5.84, P < 0.0001). Interestingly, KGS-high tumors revealed enrichments in TGF-ß and WNT-ß catenin signaling pathways, exhibited heightened activation of the epithelial-mesenchymal transition (EMT) pathway and proved intensified tumor stemness compared to their KGS-low counterparts. Additionally, KGS-high tumor cells exhibited increased sensitivity to several targeted agents, including gefitinib, erlotinib, lapatinib, and trametinib, in comparison to KGS-low cells. Conclusion: This study developed a KGS score that independently predicts the prognosis in LUAD. High expression of KGS score, accompanied by upregulation of TGF-ß and WNT-ß catenin signaling pathways, confers more aggressive EMT and tumor progression.

Circularly Polarized Room-Temperature Phosphorescence with an Ultrahigh Dissymmetry Factor from Carbonized Polymer Dots by Stacked Chiral Photonic Films.

Developing circularly polarized room-temperature phosphorescent (CPRTP) materials with a high dissymmetry factor (glum) and long afterglow is very attractive but highly challenging. Here, a CPRTP emission featuring ultrahigh glum value and desired visualization characteristic in a bilayer composite photonic film is achieved for the first time. In the constructed system, N and P co-doped carbonized polymer dots (NP-CPDs) are dispersed into polyvinyl alcohol (PVA) as the phosphorescent emitting layer, and helically structured cholesteric polymer films are used as selective reflective layers to convert the unpolarized emission of NP-CPDs into circularly polarized emission. On the basis of the modulation of the helical structure period of the cholesteric polymer, the bilayer composite film enables NP-CPDs to obtain a high glum value. Notably, the optimized photonic film emits CPRTP with glum as high as 1.09 and a green afterglow lasting above 8.0 s. Moreover, the composite photonic array films featuring information encryption characteristics are developed by modulating the liquid crystal phase of the cholesteric polymer film and the dot coating position of the NP-CPDs/PVA layer, thus expanding the application of CPRTP materials in cryptography and anti-counterfeiting.