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1.
Med Sci Monit ; 29: e940041, 2023 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-37254466

RESUMO

BACKGROUND Endoscopic submucosal dissection (ESD) has become a preferred treatment method for patients with early esophageal cancer (EEC), but it can easily be complicated by esophageal stricture. In this study, we aimed to analyze the predictive value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR) for post-ESD esophageal stricture of EEC. MATERIAL AND METHODS A retrospective study of 285 patients with EEC who underwent ESD was conducted. Patients were divided into 2 groups according to whether there were complications of esophageal stricture: the stricture group (n=72) and the non-stricture group (n=213). A t test or chi-squared test was used to compare the clinical differences in different subgroups. Receiver operating characteristic (ROC) curves were plotted to determine and compare the predictive value of NLR, PLR, and MLR in post-ESD esophageal stricture. Spearman correlation was used to detect the relationship between NLR, PLR, and MLR and the severity of esophageal stricture. RESULTS The NLR, PLR, and MLR values in the stricture group were higher than those in the non-stricture group, and there was a positive correlation between NLR and MLR and the severity of stricture according to the Spearman correlation test (P<0.05). ROC analysis showed that the area under the ROC curve value of the combination of NLR, PLR, and MLR (0.850) was higher than the NLR (0.792), PLR (0.774), and MLR (0.768). CONCLUSIONS The combination of NLR, PLR, and MLR could help clinicians to predict post-ESD esophageal stricture in the early stage.


Assuntos
Ressecção Endoscópica de Mucosa , Neoplasias Esofágicas , Estenose Esofágica , Humanos , Neutrófilos , Monócitos , Estudos Retrospectivos , Estenose Esofágica/etiologia , Ressecção Endoscópica de Mucosa/efeitos adversos , Prognóstico , Linfócitos , Plaquetas , Neoplasias Esofágicas/cirurgia
2.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 45(2): 346-350, 2023 Apr.
Artigo em Zh | MEDLINE | ID: mdl-37157087

RESUMO

Wernekink commissure syndrome is a rare midbrain syndrome with bilateral cerebellar dysfunction,eye movement disorder,and palatal myoclonus.Few cases of this syndrome have been reported in China,let alone those combined with hallucinations and involuntary groping.This paper reports the diagnosis and treatment of a case of Wernekink commissure syndrome with hallucinations and involuntary groping,aiming to enrich the knowledge about this disease for clinicians.


Assuntos
Mesencéfalo , Transtornos da Motilidade Ocular , Humanos , Transtornos da Motilidade Ocular/diagnóstico , Medula Espinal , Síndrome , Alucinações
3.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 44(6): 1112-1116, 2022 Dec.
Artigo em Zh | MEDLINE | ID: mdl-36373635

RESUMO

Studies have revealed the neuropathological mechanism of the cognitive impairment associated with neurodegenerative diseases.However,the therapies for these cognitive disorders are limited,and the prevalence of cognitive impairment is expected to increase significantly in the future,which proves the necessity of new therapeutic agents.In recent years,the pharmacological activity of ß2-adrenergic receptor(ß2-AR)has been extensively studied,which has demonstrated that ß2-AR agonist has therapeutic effects on the cognitive impairment associated with several common neurodegenerative diseases,including Alzheimer's disease,vascular dementia,Parkinson's disease with dementia,and Lewy body dementia.We reviewed the neuropathological features of cognitive impairment in several common neurodegenerative diseases and expounded the pharmacological effects of ß2-AR on related diseases.


Assuntos
Doença de Alzheimer , Disfunção Cognitiva , Doença por Corpos de Lewy , Doenças Neurodegenerativas , Humanos , Doenças Neurodegenerativas/complicações , Doenças Neurodegenerativas/tratamento farmacológico , Doença por Corpos de Lewy/complicações , Doença por Corpos de Lewy/tratamento farmacológico , Doença por Corpos de Lewy/patologia , Disfunção Cognitiva/tratamento farmacológico , Disfunção Cognitiva/complicações , Agonistas Adrenérgicos
4.
Microb Pathog ; 152: 104618, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33220443

RESUMO

Over the past decade, various avian influenza viruses have been isolated from wild ducks found in the northeast of China. To monitor the prevalence of multiple subtype specific AIVs antibodies, 1705 wild ducks' eggs from six wetlands of northeast China were analyzed for surveillance of H1, H3, H5, and H7 AIVs antibodies by c-ELISA and HI test from Jan 2017 to Dec 2019. The results show that the combined frequency of multiple subtype specific AIVs antibodies were H1 (12.32%), H3 (8.15%), H5 (2.05%), and H7 (3.46%) respectively. Therefore, a detailed analysis of the geographical distribution of AIVs in China, and the risk factors for human infection is of vital importance. This study provides basic data for other researchers to deeply study AIVs distribution characteristics, and for governments to develop detailed measures to control the spread of AIVs.


Assuntos
Vírus da Influenza A , Influenza Aviária , Animais , China/epidemiologia , Patos , Gema de Ovo , Humanos , Influenza Aviária/epidemiologia , Filogenia
5.
Microb Pathog ; 155: 104881, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33864877

RESUMO

Herein, we conducted a meta-analysis aimed at comprehensively assessing avian influenza A (H9N2) virus infection seroprevalence and infection-related risk factors among humans in China. We reviewed published studies pertaining to H9N2 seroprevalence of in China from inception to March 20, 2020, with PubMed, Clinical Trial, VIP, CNKI and databases being used to identify English and Chinese articles. After excluding the incomplete literature and data, 45 studies about risk factor of human H9N2 viral infections in China were analyzed by systematic review and meta-analysis. Our results showed that 45 studies (59,590 total patients) met the inclusion criteria. Overall H9N2 infection seroprevalence in China was estimated to be 5.56% (95%CI = 3.88-7.28), while that from central China the seroprevalence was 22.72% (95%CI = 12.18-33.84), with this prevalence being greater than that observed in other regions. H9N2 infection seroprevalence was related to sampling time, testing methodology, gender, and other demographic factors. This review will provide a basis for further understanding the risk factors of H9N2 infections in China, and it is necessary to study how to formulate strict and targeted measures to prevent the spread of the virus.


Assuntos
Vírus da Influenza A Subtipo H9N2 , Influenza Aviária , Influenza Humana , Animais , China/epidemiologia , Humanos , Influenza Humana/epidemiologia , Estudos Soroepidemiológicos
6.
J Dairy Sci ; 104(3): 3569-3574, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33358821

RESUMO

Staphylococcus aureus is one of the most common mastitis-causing bacteria in dairy cows. It is associated with reduced production performance in animals and with huge financial losses for the dairy industry worldwide. An accurate and sensitive method for the early diagnosis and identification of Staph. aureus in milk samples is essential. The present study aimed to establish a closed-tube isothermal multiple self-matching-initiated amplification (IMSA) technique for visual confirmation of the presence of Staph. aureus targeting the nuc conserved sequence gene. The specific primers successfully amplified the target sequence within 45 min at 63°C reaction temperature and using the optimal components of the reaction system. The positive amplicon showed bright green fluorescence under UV light when mixed with the chromogenic substrate SYBR Green I, and the negative samples remained orange in color. We observed fluorescence and a ladder-like pattern in the IMSA amplicon for all Staph. aureus strains, and we observed no significant change for the non-Staph. aureus strains. The IMSA assay had high specificity compared with loop-mediated isothermal amplification (LAMP): it confirmed the presence of all 7 Staph. aureus strains, and we found no false-positive results for the 12 non-Staph. aureus strains. The lower limit of detection for the IMSA assay was 1 × 102 cfu/mL, 10-fold more sensitive than the results obtained using LAMP. We also successfully applied the IMSA assay to confirm the presence of Staph. aureus in milk samples of cows with mastitis, and the results were consistent with those of LAMP and real-time PCR. The present study reports the use of IMSA to confirm the presence of Staph. aureus and provides a potentially useful method for rapid preliminary screening for Staph. aureus.


Assuntos
Doenças dos Bovinos , Mastite Bovina , Infecções Estafilocócicas , Animais , Bovinos , Feminino , Mastite Bovina/diagnóstico , Leite , Técnicas de Diagnóstico Molecular , Técnicas de Amplificação de Ácido Nucleico , Sensibilidade e Especificidade , Infecções Estafilocócicas/veterinária , Staphylococcus aureus/genética
7.
Diabetes Metab Res Rev ; 36(7): e3319, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32233013

RESUMO

BACKGOUND: To figure out whether diabetes is a risk factor influencing the progression and prognosis of 2019 novel coronavirus disease (COVID-19). METHODS: A total of 174 consecutive patients confirmed with COVID-19 were studied. Demographic data, medical history, symptoms and signs, laboratory findings, chest computed tomography (CT) as well the treatment measures were collected and analysed. RESULTS: We found that COVID-19 patients without other comorbidities but with diabetes (n = 24) were at higher risk of severe pneumonia, release of tissue injury-related enzymes, excessive uncontrolled inflammation responses and hypercoagulable state associated with dysregulation of glucose metabolism. Furthermore, serum levels of inflammation-related biomarkers such as IL-6, C-reactive protein, serum ferritin and coagulation index, D-dimer, were significantly higher (P < .01) in diabetic patients compared with those without, suggesting that patients with diabetes are more susceptible to an inflammatory storm eventually leading to rapid deterioration of COVID-19. CONCLUSIONS: Our data support the notion that diabetes should be considered as a risk factor for a rapid progression and bad prognosis of COVID-19. More intensive attention should be paid to patients with diabetes, in case of rapid deterioration.

8.
Nutr Metab Cardiovasc Dis ; 30(7): 1061-1067, 2020 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-32456948

RESUMO

BACKGROUND AND AIM: A novel coronavirus severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) caused pneumonia, Coronavirus Disease 2019 (COVID-19), broke out in Wuhan, China in December 2019, and spread all over the world. Patients with COVID-19 showed huge differences in the hospital stay, progression, and prognosis. As reported, the comorbidities may play an important role in COVID-19. Here, we aim to address the role of cardiovascular disease (CVD) in the progression and prognosis of COVID-19. METHODS AND RESULTS: Eighty-three confirmed COVID-19 patients were divided into CVD (n = 42) and non-CVD (n = 41) group according to their medical history. Medical records including demographic data, medical history, clinical characteristics, laboratory examinations, chest computed tomography (CT), and treatment measures were collected, analyzed, and compared between the two groups. COVID-19 patients with CVD showed (1) more severe pathological changes in the lungs, (2) elevated injury-related enzymes including α-hydroxybutyrate dehydrogenase (HDBH), lactic dehydrogenase (LDH), γ-glutamyltransferase (GGT), creatine kinase (CK), and alanine aminotransferase (ALT), (3) significantly increased uncontrolled inflammation related markers, such as c-reactive protein (CRP), interleukin (IL)-6, serum ferritin, erythrocyte sedimentation rate (ESR), and serum amyloid A (SAA), (4) serious hypercoagulable status reflected by increased D-dimer and serum fibrinogen (FIB), and (5) higher mortality, compared to COVID-19 patients without CVD. CONCLUSIONS: Our data indicated that CVD is a strong risk factor for rapid progression and bad prognosis of COVID-19. More intensive medical care should be applied to patients with CVD to prevent rapid deterioration of the disease.


Assuntos
Betacoronavirus , Doenças Cardiovasculares/epidemiologia , Causas de Morte , Infecções por Coronavirus/epidemiologia , Surtos de Doenças/estatística & dados numéricos , Progressão da Doença , Pneumonia Viral/epidemiologia , Adulto , Idoso , Biomarcadores/sangue , Análise Química do Sangue , Proteína C-Reativa/metabolismo , COVID-19 , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/terapia , China/epidemiologia , Estudos de Coortes , Comorbidade , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/terapia , Bases de Dados Factuais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/diagnóstico , Pneumonia Viral/terapia , Valores de Referência , Estudos Retrospectivos , SARS-CoV-2 , Análise de Sobrevida , Tomografia Computadorizada por Raios X/métodos
9.
BMC Genomics ; 18(1): 949, 2017 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-29212448

RESUMO

BACKGROUND: Chlamydia (C.) gallinacea is a recently identified bacterium that mainly infects domestic chickens. Demonstration of C. gallinacea in human atypical pneumonia suggests its zoonotic potential. Its prevalence in chickens exceeds that of C. psittaci, but genetic and genomic research on C. gallinacea is still at the beginning. In this study, we conducted whole-genome sequencing of C. gallinacea strain JX-1 isolated from an asymptomatic chicken, and comparative genomic analysis between C. gallinacea strains and related chlamydial species. RESULTS: The genome of C. gallinacea JX-1 was sequenced by single-molecule, real-time technology and is comprised of a 1,059,522-bp circular chromosome with an overall G + C content of 37.93% and sequence similarity of 99.4% to type strain 08-1274/3. In addition, a plasmid designated pJX-1, almost identical to p1274 of the type strain, except for two point mutations, was only found in field strains from chicken, but not in other hosts. In contrast to chlamydial species with notably variable polymorphic membrane protein (pmp) genes and plasticity zone (PZ), these regions were conserved in both C. gallinacea strains. There were 15 predicted pmp genes, but only B, A, E1, H, G1 and G2 were apparently intact in both strains. In comparison to chlamydial species where the PZ may be up to 50 kbp, C. gallinacea strains displayed gene content reduction in the PZ (14 kbp), with strain JX-1 having a premature STOP codon in the cytotoxin (tox) gene, while tox gene is intact in the type strain. In multilocus sequence typing (MLST), 15 C. gallinacea STs were identified among 25 strains based on cognate MLST allelic profiles of the concatenated sequences. The type strain and all Chinese strains belong to two distinct phylogenetic clades. Clade of the Chinese strains separated into 14 genetically distinct lineages, thus revealing considerable genetic diversity of C. gallinacea strains in China. CONCLUSIONS: In this first detailed comparative genomic analysis of C. gallinacea, we have provided evidence for substantial genetic diversity among C. gallinacea strains. How these genetic polymorphisms affect C. gallinacea biology and pathogenicity should be addressed in future studies that focus on phylogenetics and host adaption of this enigmatic bacterial agent.


Assuntos
Proteínas de Bactérias/genética , Galinhas , Infecções por Chlamydia/veterinária , Chlamydia/genética , Variação Genética , Genoma Bacteriano , Doenças das Aves Domésticas/microbiologia , Animais , China , Chlamydia/patogenicidade , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/genética , Infecções por Chlamydia/microbiologia , Genótipo , Epidemiologia Molecular , Tipagem de Sequências Multilocus/métodos , Filogenia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/genética , Análise de Sequência de DNA/métodos
10.
Opt Express ; 25(12): 13455-13464, 2017 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-28788890

RESUMO

The theoretical proposal for an improved Talbot imaging technique has been analyzed in a periodic object illuminated by a pseudo-thermal light source and detected only by two detectors in two imaging schemes. The improved effect of lensless grating ghost image and Talbot image quality (fringe resolution and visibility) can be attributed to the higher correlation orders N. While at a certain order N, the spatial resolution of Talbot carpet patterns is determined by two-photon bunching effect, which depends on the positions of the periodic object and two detectors and different detection methods. Moreover, the sub wavelength of spatial correlation peaks and Talbot images obtained, when we use two asynchronous scanning detectors or an accelerated scanning detector. The present imaging schemes have the characteristic of the controllable image visibility and resolution, which has a potential application in periodic image reconstructions, sub wavelength resolution microscopy and sub wavelength atom lithography.

11.
iScience ; 27(2): 108635, 2024 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-38292426

RESUMO

The beneficial effects of physical exercise on human cardiorespiratory fitness might be through reduced systemic inflammation, but the mechanism remains a controversy. Recent studies have highlighted the importance of spleen microbiomes in immune regulation. Hence, we conducted a study using a high-fat diet and exercise mouse model to investigate the relationships among different exercise intensities, spleen microbiome composition, and cardiac function. The mice spleen contained a diverse array of microbiota. Different intensities of exercise resulted in varying compositions of the spleen microbiome, Treg cell levels, and mouse heart function. Additionally, the abundance of Lactobacillus johnsonii in the mouse spleen exhibited a positive correlation with Treg cell levels, suggesting that Lactobacillus johnsonii may contribute to the production of Treg cells, potentially explaining the protective role of moderate-intensity exercise on cardiac function. In conclusion, our findings provide evidence that moderate-intensity exercise may promote cardiac function protection by influencing the spleen microbiome composition.

12.
J Med Biochem ; 43(2): 234-242, 2024 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-38699697

RESUMO

Background: It is an important clinical means to identify benign and malignant breast diseases caused by nipple discharge through the detection and analysis of components in nipple discharge. This study was aimed to test the expression and clinical significance of carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153) and carcinoembryonic antigen (CEA) in nipple discharge of breast cancer patients. Methods: From January 2017 to December 2018, 86 patients with invasive ductal carcinoma of the breast with nipple discharge (breast cancer group) and 50 patients with ordinary breast duct hyperplasia with nipple discharge (benign control group) were selected, and the levels of CA125, CA153 and CEA in nipple discharge and serum were detected by electrochemiluminescence immunoassay.

13.
Int Immunopharmacol ; 114: 109452, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36446236

RESUMO

Endometriosis remains a widespread but severe gynecological disease in women of reproductive age, with an unknown etiology and few treatment choices. The menstrual reflux theory is largely accepted as the underlying etiology but does not explain the morbidity or unpleasant pain sensations of endometriosis. The neurological and immune systems are both involved in pain mechanisms of endometriosis, and interlinked through a complex combination of cytokines and neurotransmitters. Numerous pieces of evidence suggest that the nerve injury-inducible protein, Ninjurin, is actively expressed in endometriosis lesions, which contributes to the etiology and development of endometriosis. It may be explored in the future as a novel therapeutic target. The aim of the present review was to elucidate the multifaceted role of Ninjurin. Furthermore, we summarize the association of Ninjurin with the pain mechanism of endometriosis and outline the future research directions. A novel therapeutic pathway can be discovered based on the potential pathogenic variables.


Assuntos
Endometriose , Humanos , Feminino , Dor , Menstruação , Proteínas do Tecido Nervoso , Citocinas
14.
Clin Ther ; 45(10): 973-976, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-37599165

RESUMO

PURPOSE: The goal of this study was to examine the efficacy and safety of the levonorgestrel intrauterine system (LNG-IUS) versus dienogest (DNG) in female subjects with symptomatic uterine adenomyosis. METHODS: This study enrolled 117 women with symptomatic adenomyosis who visited our hospital from May 1, 2019, to June 30, 2022. Participants were randomized to either the LNG-IUS group (n = 48) or the DNG group (n = 79) in an as-controlled clinical trial for 36 months. Visual analog scale (VAS) scores, uterine volume, endometrial thickness, serum carcinoma antigen 125 level, estradiol, follicle-stimulating hormone, luteinizing hormone, and side effects were assessed to compare the efficacy of LNG-IUS and DNG. FINDINGS: The VAS pain score was significantly decreased in both groups after 3 months of treatment. Three months later, patients receiving DNG reported significantly lower VAS scores compared with those treated with LNG- IUS (P < 0.05). Compared with LNG-IUS, DNG effectively controlled uterine volume growth after 12 months of treatment but neither significantly reduced uterine volume. During the treatment period, endometrial thickness in both groups was maintained at 0.4 to 0.7 cm. IMPLICATIONS: Both DNG and LNG-IUS significantly improved adenomyosis-associated pain after 3 months of treatment. Compared with LNG-IUS, DNG was shown to continuously relieve the symptoms of pain and effectively control the growth of uterine volume.


Assuntos
Adenomiose , Nandrolona , Feminino , Humanos , Levanogestrel/efeitos adversos , Adenomiose/tratamento farmacológico , Adenomiose/induzido quimicamente , Adenomiose/complicações , Nandrolona/efeitos adversos , Dor/tratamento farmacológico
15.
Front Oncol ; 12: 861959, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35600411

RESUMO

Lung cancer is characterized by the most common oncological disease and leading cause of cancer death worldwide, of which a group of subtypes known as non-small cell lung cancer (NSCLC) accounts for approximately 85%. In the past few decades, important progression in the therapies of NSCLC has enhanced our understanding of the biology and progression mechanisms of tumor. The application of immunotherapy and small molecule tyrosine kinase inhibitors has brought significant clinical benefits in certain patients. However, early metastasis and the emergence of resistance to antitumor therapy have resulted in the relatively low overall cure and survival rates for NSCLC. Autophagy is a conserved process that allows cells to recycle unused or damaged organelles and cellular components. It has been reported to be related to the progression of NSCLC and resistance to targeted therapy and cytotoxic chemotherapy. Therefore, autophagy is considered as a potential therapeutic target for NSCLC. Mounting results have been reported about the combination of tyrosine kinase inhibitors and inhibitors of autophagy in models of NSCLC. This review aims to provide a comprehensive review on the roles of autophagy in NSCLC, focusing on related clinical data of agents that regulate autophagy in NSCLC. Furthermore, this study will provide a theoretical basis for further improvement of autophagy-based cancer therapy.

16.
Front Cell Infect Microbiol ; 12: 844132, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35601099

RESUMO

The susceptibility of mice to hepatitis B virus (HBV) infection depends on their genetic background. The gut microbiota modulates the antiviral immune response in the liver and plays a protective role against HBV infection. However, whether HBV infection outcomes depend on the gut microbiota remains unclear. In this study, we assessed the gut microbiota composition in naïve BALB/c and C57BL/6 mice using 16S rRNA gene sequencing. The gut microbiota in BALB/c mice was depleted using broad-spectrum antibiotics (ABX) and then reconstituted with fecal microbiota from naïve BALB/c or C57BL/6 mice to evaluate the effect of fecal microbiota transplantation (FMT) on the outcomes of and immune response to HBV infection. We found that HBV infection outcomes and the gut microbiota composition differed between BALB/c and C57BL/6 mice. Commensal bacteria from the fecal microbiota selectively colonized the guts of ABX-treated BALB/c mice. Mice receiving fecal microbiota from BALB/c or C57BL/6 mice displayed different HBV infection outcomes. The fecal microbiota from C57BL/6 mice induced immune tolerance in the liver and prolonged HBV infection. In conclusion, HBV infection outcomes in mice are determined by the host genetic background and gut microbiota composition. Reconstitution of the gut microbiota by FMT can alter the susceptibility to HBV infection in mice.


Assuntos
Transplante de Microbiota Fecal , Hepatite B , Animais , Fezes/microbiologia , Hepatite B/terapia , Vírus da Hepatite B/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , RNA Ribossômico 16S/genética
17.
Comput Math Methods Med ; 2022: 8739551, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35281951

RESUMO

Objectives: The article is aimed at investigating the suture effect of absorbable suture in strabismus correction and the advantage of Watson care theory. Methods: 148 children with strabismus were enrolled in this study and divided into two groups. The control group received routine nursing, and on this basis, the observation group was given Watson care theory nursing and eye muscle suture with absorbable sutures. The degree of cooperation (induction compliance checklist (ICC) score), negative emotion (modified Yale preoperative anxiety scale (m-YAPS) score), pain (face pain scale (FPS) score), quality of nursing management, parents' satisfaction, clinical efficacy, and adverse reactions were compared. Results: After nursing and surgical treatment, all children's strabismus symptoms were improved, and the improvement of strabismus and visual function in the observation group was more obvious. Watson care nursing can effectively improve the nursing management quality, parents' satisfaction, children's negative emotions, and treatment compliance. Conclusions: As a result, Watson care theory nursing is of great significance for improving the relationship between nurses and patients and building a harmonious hospital.


Assuntos
Teoria de Enfermagem , Estrabismo/enfermagem , Estrabismo/cirurgia , Suturas , Criança , Pré-Escolar , Biologia Computacional , Feminino , Humanos , Masculino , Complicações Pós-Operatórias/etiologia , Qualidade da Assistência à Saúde , Estrabismo/fisiopatologia , Técnicas de Sutura/enfermagem
18.
Genomics Proteomics Bioinformatics ; 20(2): 288-303, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35609771

RESUMO

Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis. Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects. Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects. We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation. CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability. Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis. Notably, all the therapeutic effects were exerted in a gut microbiota-dependent manner. Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects. In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.


Assuntos
Colite Ulcerativa , Colite , Microbioma Gastrointestinal , Camundongos , Animais , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Sulfato de Dextrana/efeitos adversos , RNA Ribossômico 16S/genética , Colite/tratamento farmacológico , Colite/metabolismo , Anti-Inflamatórios/efeitos adversos , Inflamação
19.
Cancers (Basel) ; 14(19)2022 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-36230568

RESUMO

Taraxasterol (TAX), one of the active components in Dandelion, demonstrated strong antitumor properties in several cancers. However, the effect and underlying mechanism of TAX in non-small cell lung cancer (NSCLC) is unclear. In this study, we showed that TAX inhibited the proliferation of cells by inducing S-phase cell cycle arrest and prevented cell migration by interfering epithelial-mesenchymal transition (EMT) in Lewis lung cancer (LLC) cells and lung carcinoma SPC-A1 cells. The pharmacological network analysis predicted that induction of apoptosis might be the potential mechanism of TAX-mediated cell deaths. Further in vitro experiments showed that TAX could significantly induce cancer cell apoptosis as verified by increased pro-apoptotic molecules including Bax, caspase-9, and PARP1 downregulated anti-apoptotic protein Bcl-2; and decreased mitochondrial potential. The LLC subcutaneous tumor model demonstrated that TAX inhibited tumor growth by induction of apoptosis and inhibition of proliferation in vivo, which is consistent with the in vitro data. Importantly, TAX administration downregulated the proportion of Treg cells and upregulated CD107a+ NK cells in the tumor microenvironment in the tumor model. Together, these data reveal that TAX performs its antitumor effect by inducing apoptosis and modulating the tumor microenvironment, providing evidence that TAX could serve as a potential natural drug for lung cancer therapy.

20.
Int J Biol Sci ; 18(4): 1381-1397, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35280677

RESUMO

Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colon, and it has become one of the world-recognized medical problems as it is recurrent and refractory. Berberine (BBR) is an effective drug for UC treatment. However, the underlying mechanism and targets remain obscure. In this study, we systematically investigated the therapeutic effect and its mechanism of BBR in ameliorating DSS-induced mouse colitis. Expectedly, the colon inflammation was significantly relieved by BBR, and microbiota depletion by antibiotic cocktail significantly reversed the therapeutic effect. Further studies showed that BBR can regulate the abundance and component of bacteria, reestablish the broken chemical and epithelial barriers. Meanwhile, BBR administration dramatically decreased ILC1 and Th17 cells, and increased Tregs as well as ILC3 in colonic tissue of DSS-induced mice, and it was able to regulate the expression of various immune factors at the mRNA level. Moreover, a proteomic study revealed that Wnt/ß-catenin pathway was remarkably enhanced in colonic tissue of BBR-treated mice, and the therapeutic effect of BBR was disappeared after the intervention of Wnt pathway inhibitor FH535. These results substantially revealed that BBR restores DSS-induced colon inflammation in a microbiota-dependent manner, and BBR performs its protective roles in colon by maintaining the structure and function of the intestinal mucosal barrier, regulating the intestinal mucosal immune homeostasis and it works through the Wnt/ß-catenin pathway. Importantly, these findings also provided the proof that BBR serves as a potential gut microbiota modulator and mucosal barrier protector for UC prevention and therapy.


Assuntos
Berberina , Colite Ulcerativa , Colite , Microbioma Gastrointestinal , Animais , Berberina/efeitos adversos , Berberina/farmacologia , Berberina/uso terapêutico , Colite/induzido quimicamente , Colite/tratamento farmacológico , Colite/metabolismo , Colite Ulcerativa/metabolismo , Colo/metabolismo , Sulfato de Dextrana , Modelos Animais de Doenças , Inflamação/metabolismo , Mucosa Intestinal/metabolismo , Camundongos , Proteômica , Via de Sinalização Wnt , beta Catenina/metabolismo
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