Labeling of anti-MUC-1 binding single chain Fv fragments to surface modified upconversion nanoparticles for an initial in vivo molecular imaging proof of principle approach.

In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF(4):Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach.

In-vivo imaging of the uptake of upconversion nanoparticles by plant roots.

Watering of Phalaenopsis and Arabidopsis plants with an aqueous colloidal solution of NaYF4:Yb,Er nanoparticles leads to uptake and transport of nanoparticles into the plants within a few days. Characteristic upconversion emission of the particles can be excited in the shoot and the leaves of the plants. Uptake of particles by the root was studied by confocal laser-scanning microscopy using excitation in the near-infrared. We demonstrated that nanoparticles are able to enter the stele of the plant root and thus the long distance transport system despite their relatively large size in comparison to the size of the structures responsible for the transport.