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Flexible and lightweight pressure sensors have attracted tremendous attention as a promising component of wearable biological motion sensors and artificial electronic skins. Here, the electromechanical response of as-electrospun fiber mats composed of a commodity polymer, atactic polystyrene, which can be applied in low-cost/large-area, flexible, and lightweight pressure sensors is demonstrated. The fiber mat demonstrates a significantly high apparent converse piezoelectric constant of >30 000 pm V-1 under static measurement and ≈13 000 pm V-1 even at a high frequency of 1 kHz. The first theoretical model to explain the unique electromechanical response is constructed, which reveals that the softness and moderate charge of the fiber mat are the reasons for the significantly high electromechanical response. Further, apparent piezoelectric constants obtained by direct measurement are lower than those obtained by the converse measurement, which is attributed to the densification and hardening of the fiber mat due to prepressure applied in direct measurement. These findings are likely to serve as a milestone for the development of large-area, flexible, and lightweight pressure sensors at low cost, as well as highly movable actuators like optical modulators without a substantial mechanical load.
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Poliestirenos , Pele Artificial , Modelos Teóricos , PolímerosRESUMO
BACKGROUND: Cancer stem cells (CSCs) have enhanced mechanisms of protection from oxidative stress. A variant form of CD44 (CD44v), a major CSC marker, was shown to interact with xCT, a subunit of cystine-glutamate transporter, which maintains high levels of intracellular reduced glutathione (GSH) which defend the cell against oxidative stress. Sulfasalazine (SSZ) is an inhibitor of xCT and was shown to suppress the survival of CD44v-positive stem-like cancer cells both in vitro and in vivo. To find the dose of SSZ which can safely reduce the population of CD44v-positive cells in tumors, a dose-escalation study in patients with advanced gastric cancer was conducted. METHODS: SSZ was given four times daily by oral administration with 2 weeks as one cycle. Tumor biopsies were obtained before and after 14 days of administration of SSZ to evaluate expression of CD44v and the intratumoral level of GSH. RESULTS: Eleven patients were enrolled and received a dosage from 8 to 12 g/day. Safety was confirmed up to a dosage of 12 g/day, which was considered the maximum tolerated dose. Among the eight patients with CD44v-positive cells in their pretreatment biopsy samples, the CD44v-positive cancer cell population appeared to be reduced in the posttreatment biopsy tissues of four patients. Intratumoral GSH levels were also decreased in two patients, suggesting biological effectiveness of SSZ at 8 g/day or greater. CONCLUSIONS: This is the first study of SSZ as an xCT inhibitor for targeting CSCs. Reduction of the levels of CD44v-positive cells and GSH was observed in some patients, consistent with the mode of action of SSZ in CSCs.
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Receptores de Hialuronatos/metabolismo , Neoplasias Hepáticas/tratamento farmacológico , Células-Tronco Neoplásicas/efeitos dos fármacos , Neoplasias Peritoneais/tratamento farmacológico , Neoplasias Gástricas/tratamento farmacológico , Sulfassalazina/uso terapêutico , Adulto , Idoso , Idoso de 80 Anos ou mais , Anti-Inflamatórios não Esteroides/farmacocinética , Anti-Inflamatórios não Esteroides/uso terapêutico , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/secundário , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Células-Tronco Neoplásicas/metabolismo , Células-Tronco Neoplásicas/patologia , Neoplasias Peritoneais/metabolismo , Neoplasias Peritoneais/secundário , Prognóstico , Espécies Reativas de Oxigênio/metabolismo , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia , Sulfassalazina/farmacocinética , Distribuição Tecidual , Adulto JovemRESUMO
The electrochemical detection of dissolved ozone in water was examined using boron-doped diamond (BDD) electrodes. A well-defined reduction peak was observed at ~380 mV for H-terminated BDD, whereas it was observed at ~200 mV in the case of O-terminated BDD for an ozone solution in a 0.1 M phosphate buffer solution at pH 7. The peak potential for ozone reduction was selective with respect to oxygen reduction at both H- and O-terminated BDD electrodes, whereas it occurred at approximately the same potential as oxygen reduction at other types of solid electrodes, including glassy-carbon, platinum, and gold electrodes. Interference from chlorine was not observed in lower concentration than 300 µM ClO(-). Furthermore, in order to apply the detection technique to electrolyte-free media, BDD microelectrodes were also used. A linear calibration curve for dissolved ozone in water could be achieved between concentrations of 0.49 and 740 µM, with an estimated detection limit (S/N = 3) of 0.185 µM (S/N = 3). Excellent stability was demonstrated for repetitions of these calibration curves performed in 3 consecutive days.
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Boro/química , Ozônio/análise , Técnicas Eletroquímicas , Eletrodos , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
As-electrospun microfiber mats comprising atactic polystyrene (aPS), a low-cost commodity polymer, have demonstrated beneficial electromechanical properties. However, the variability of the electromechanical properties of fiber mats produced using different electrospinning conditions has not been investigated. Therefore, herein, the direct electromechanical properties of aPS fiber mats produced using different deposition times (tdep) and electrospinning voltages (VES) are investigated. The resulting apparent piezoelectric d constant (dapp) of the fiber mats demonstrates a specific peak value for tdep as high as ~1600 pC N-1 under 1-kPa pressure application after ~0.2-kPa pre-pressure application, although the dapp of the fiber mats produced with some conditions is nearly zero pC·N-1. Furthermore, the peak position of dapp with tdep is fundamentally determined with σEff0/YD(h-hpre) [σEff0: effective surface charge density, YD(h-hpre): secant modulus of elasticity]. Charge distribution models for fiber mats with different tdep are established. The models explain the characteristics of the significant changes in YD(h-hpre) and σEff0 with tdep. These findings provide significant directions for the production of fiber mats with improved direct electromechanical properties.
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A variety of crystal structures is found in nature, not only equilibrium structures reflecting molecular structures, but also non-equilibrium structures which depend on the physicochemical conditions occurring during the crystal growth. In this paper, we focus on the dendrite structure of sodium chloride (NaCl) formed by the simple evaporation of an aqueous NaCl solution. The characteristics of the growth structures were measured as a function of the initial concentration of NaCl. In addition, the crystal growth process was measured using optical microscopy. As a result, the growth rate was not constant but was found to oscillate over time and synchronize with the wetting of the crystal. Our observations indicate that dendrite structures are more easily generated at higher initial concentrations. The detailed mechanism for dendrite pattern formation is still under investigation.
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Tactile sensors are widely used by the robotics industries over decades to measure force or pressure produced by external stimuli. Piezoelectric-based pressure sensors have intensively been investigated as promising candidates for tactile sensing applications. In contrast, piezoelectric-based pressure sensors are expensive due to their high cost of manufacturing and expensive base materials. Recently, an effect similar to the piezoelectric effect has been identified in non-piezoelectric polymers such as poly(d,l-lactic acid (PDLLA), poly(methyl methacrylate) (PMMA) and polystyrene. Hence investigations were conducted on alternative materials to find their suitability. In this article, we used inexpensive atactic polystyrene (aPS) as the base polymer and fabricated functional fibers using an electrospinning method. Fiber morphologies were studied using a field-emission scanning electron microscope and proposed a unique pressure sensor fabrication method. A fabricated pressure sensor was subjected to different pressures and corresponding electrical and mechanical characteristics were analyzed. An open circuit voltage of 3.1 V was generated at 19.9 kPa applied pressure, followed by an integral output charge (ΔQ), which was measured to calculate the average apparent piezoelectric constant dapp and was found to be 12.9 ± 1.8 pC N-1. A fabricated pressure sensor was attached to a commercially available robotic arm to mimic the tactile sensing.
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Cytochrome P450 2B6 (CYP2B6) is a potentially important enzyme for the metabolism of clinical drugs, and it exhibits genetic polymorphism. Thus far, 29 allelic variants of CYP2B6 (CYP2B6*1-CYP2B6*29) have been identified. This study aimed to investigate whether 26 of the variant alleles of CYP2B6 (CYP2B6*2-CYP2B6*21 and CYP2B6*23-CYP2B6*28) affect its kinetics in the metabolism of 7-ethoxy-4-trifluoromethylcoumarin (7-EFC) and selegiline. Wild-type CYP2B6.1 and the allelic variants were heterologously expressed in COS-7 cells. In-vitro kinetic analysis revealed that when compared with the wild-type protein CYP2B6.1, CYP2B6.10 and CYP2B6.14 exhibited significantly lower V(max)/K(m) values for selegiline N-demethylation. The kinetic parameters of CYP2B6.8, CYP2B6.11, CYP2B6.12, CYP2B6.13, CYP2B6.15, CYP2B6.18, CYP2B6.21, CYP2B6.24, and CYP2B6.28 could not be determined because these enzymes were inactive in the deethylation of 7-EFC and the N-demethylation/N-depropagylation of selegiline. These findings provide useful information for further genotype-phenotype studies on interindividual differences in the metabolism of CYP2B6 substrate drugs.
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Alelos , Hidrocarboneto de Aril Hidroxilases/genética , Oxirredutases N-Desmetilantes/genética , Animais , Células COS , Chlorocebus aethiops , Citocromo P-450 CYP2B6 , Humanos , Cinética , Proteínas Mutantes/genéticaRESUMO
To sense hydrophilic anions in protic solvents, we fabricated polymethylmethacrylate (PMMA) nanofibers incorporating 4-nitrophenyl azo thiourea polymer as a chromoionophore. When methanol solutions containing anions contacted the PMMA nanofiber, a bathochromic shift from 386â¯nm was observed in the absorption maximum of the chromoionophore. This spectral change is due to hydrogen bond formation between the urea moiety of the thiourea-based polymer and anions penetrating the nanofiber. This spectral change was not observed in PMMA film incorporating the same anion sensor, and the difference is attributed to the much larger specific surface area of the nanofiber compared to the film. As a result, many anions could react with the anion-sensing polymers in the nanofiber and induce a large spectral response.
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Magnetic field effects on a radical ion pair have been studied to investigate the diffusion of the radical ions generated by a photoinduced electron transfer reaction in ionic liquids having short and long alkyl chains. The yield of an escaped radical ion was evaluated by using a nanosecond laser flash photolysis under various magnetic fields. The magnitude of the magnetic field effect on the yield of the escaped radical was linearly increased with increasing solvent viscosity. Such solvent viscosity dependence of the magnetic field effect can be explained with the solvent viscosity dependence of the escape rate of the radical ions from the pair. In the time window (>20 ns) of our measurements, the effect of long alkyl chain aggregation on the dynamics of the radical ions was not clearly observed.
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Wet-electrospun (WES) polymer micron and submicron fibers are promising building blocks for small, flexible optical fiber devices, such as waveguides, sensors, and lasers. WES polymer fibers have an inherent cylindrical geometry similar to that of optical fibers and a relatively large aspect ratio. Furthermore, WES fibers can be produced using low-cost and low-energy manufacturing techniques with large-area fabrication and a large variety of materials. However, the high propagation loss in the fibers, which is normally on the order of tens or thousands of decibels per centimeter in the visible light region, has impeded the use of these fibers in optical fiber devices. Here, the origin of propagation losses is examined to develop a comprehensive and versatile approach to reduce these losses. The excess light scattering that occurs in fibers due to their inhomogeneous density is one of the primary factors in the propagation loss. To reduce this loss, the light transmission characteristics were investigated for single WES polymer fibers heated at different temperatures. The propagation loss was significantly reduced from 17.0 to 8.1 dB cm-1 at 533 nm wavelength, by heating the fibers above their glass transition temperature, 49.8 °C. In addition, systematic verification of the possible loss factors in the fibers confirmed that the propagation loss reduction could be attributed to the reduction of extrinsic excess scattering loss. Heating WES polymer fibers above their glass transition temperature is a versatile approach for reducing the propagation loss and should be applicable to a variety of WES fibers. This finding paves the way for low-loss WES fiber waveguides and their subsequent application in small, flexible optical fiber devices, including waveguides, sensors, and lasers.
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Real-time recording of the kinetics of systemically administered drugs in in vivo microenvironments may accelerate the development of effective medical therapies. However, conventional methods require considerable analyte quantities, have low sampling rates and do not address how drug kinetics correlate with target function over time. Here, we describe the development and application of a drug-sensing system consisting of a glass microelectrode and a microsensor composed of boron-doped diamond with a tip of around 40 µm in diameter. We show that, in the guinea pig cochlea, the system can measure-simultaneously and in real time-changes in the concentration of bumetanide (a diuretic that is ototoxic but applicable to epilepsy treatment) and the endocochlear potential underlying hearing. In the rat brain, we tracked the kinetics of the drug and the local field potentials representing neuronal activity. We also show that the actions of the antiepileptic drug lamotrigine and the anticancer reagent doxorubicin can be monitored in vivo. Our microsensing system offers the potential to detect pharmacological and physiological responses that might otherwise remain undetected.
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All-optical logic circuits using surface plasmon polaritons have a potential for high-speed information processing with high-density integration beyond the diffraction limit of propagating light. However, a number of logic gates that can be cascaded is limited by complicated signal phase adjustment. In this study, we demonstrate a half-adder operation with simple phase adjustment using plasmonic multimode interference (MMI) devices, composed of dielectric stripes on a metal film, which can be fabricated by a complementary metal-oxide semiconductor (MOS)-compatible process. Also, simultaneous operations of XOR and AND gates are substantiated experimentally by combining 1 × 1 MMI based phase adjusters and 2 × 2 MMI based intensity modulators. An experimental on-off ratio of at least 4.3 dB is confirmed using scanning near-field optical microscopy. The proposed structure will contribute to high-density plasmonic circuits, fabricated by complementary MOS-compatible process or printing techniques.
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A poly-DL-lactide (PLA) fiber film was prepared using the electrospinning method. This film consisted of randomly oriented PLA nanofibers. Consequently, it had sponge-like structure and was quite soft compared to PLA films prepared by spin coating. The average diameter of the fibers and the density of the film were 730 nm and 20%, respectively. By applying a voltage, the PLA film was subjected to electric-field-induced strain: expansion and compression in the thickness direction. When a voltage of -200 V was applied to the film, its thickness shrank from 13.5 µm to 10.0 µm (a 26% reduction). Electric-field-induced strain can occur via two different mechanisms: The first is electrostrictive behavior. That. is, in a highly electric field region, a change of film thickness occurs (compression only) from the electrostatic force between electrodes. The second mechanism is piezoelectric-like behavior that occurs in racemic PLA, wherein a PLA nanofiber is expanded and compressed by applying positive and negative voltage. Such piezoelectric-like behavior was not observed in spin-coated PLA films.