1.
J Clin Microbiol
; 47(4): 1238-40, 2009 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19193839
RESUMO
Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.