RESUMO
BACKGROUND AND OBJECTIVE: Periodontal disease has been associated with cardiovascular disease in the general population. It is unknown whether IgG antibody levels for periodontal pathogens are associated with the diagnosis of coronary artery disease (CAD) in HIV-positive individuals. MATERIAL AND METHODS: Twenty-four HIV-positive individuals (cases) with stored plasma available in the 12 months before CAD diagnosis were age- and sex-matched 1:2 with 46 HIV-positive individuals without CAD (controls). Antibody levels to whole cell extracts from periodontal pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum, as well as markers of inflammation sCD14, CXCL10 and high-sensitivity C-reactive protein, were compared between cases and controls using enzyme-linked immunosorbent assays. RESULTS: P. gingivalis-specific IgG levels (µg/mL) were significantly higher in individuals with CAD (median 1.48 [IQR 1.06-2.05]) compared to controls (0.70 [IQR 0.35-1.24], P<.001), and remained significantly higher following adjustment for traditional cardiovascular risk factors and HIV viral load (OR 21.6 [95% CI 3.73-125.63] P=.001). There was a borderline association between A. actinomycetemcomitans IgG antibody levels (cases, median 3.86 [IQR 3.19-4.72]; controls, 3.34 [IQR 2.59-4.07], P=.050) and no association found between F. nucleatum antibody levels and CAD. sCD14 levels (µg/mL) were higher in cases compared with controls (median 3.45 [IQR 3.03-4.11] vs 2.65 [IQR 2.32-2.99] P<.001), while CXCL10 (median 127 pg/mL [IQR 88-157] vs 153 [IQR 90-244] P=.321) and high-sensitivity C-reactive protein (median 3.44 mg/L [1.98-5.32] vs 1.85 [1.13-6.88] P=.203) levels were not different between cases and controls. CONCLUSION: Periodontal bacteria may be contributing to CAD risk in HIV-positive individuals.
Assuntos
Anticorpos Antibacterianos/sangue , Biomarcadores/sangue , Doença da Artéria Coronariana/diagnóstico , Infecções por HIV/complicações , Doenças Periodontais/microbiologia , Porphyromonas gingivalis/imunologia , Adulto , Aggregatibacter actinomycetemcomitans/imunologia , Aggregatibacter actinomycetemcomitans/patogenicidade , Antígenos de Bactérias/imunologia , Austrália , Proteína C-Reativa , Estudos de Casos e Controles , Quimiocina CXCL10/sangue , Doença da Artéria Coronariana/sangue , Doença da Artéria Coronariana/complicações , Feminino , Fusobacterium nucleatum/imunologia , Fusobacterium nucleatum/patogenicidade , Humanos , Imunoglobulina G/sangue , Inflamação/imunologia , Receptores de Lipopolissacarídeos/sangue , Masculino , Pessoa de Meia-Idade , Doenças Periodontais/complicações , Projetos Piloto , Porphyromonas gingivalis/patogenicidade , Medição de Risco , Fatores de Risco , Carga ViralRESUMO
Colony-stimulating factor 1 (CSF-1) triggers the activation of intracellular proteins in macrophages through selective assembly of signalling complexes. The separation of multimeric complexes of the CSF-1 receptor (CSF-1R) by anion-exchange chromatography enabled the enrichment of low-stoichiometry complexes. A significant proportion of the receptor in CSF-1-stimulated cells that neither possessed detectable tyrosine kinase activity nor formed complexes was separated from the receptor pool displaying autokinase activity that formed chromatographically distinct multimeric complexes. A small pool of CSF-1R formed a multimeric complex with phosphatidylinositol-3 kinase (PI-3 kinase), SHP-1, Grb2, Shc, c-Src, Cbl, and a significant number of tyrosine-phosphorylated proteins in CSF-1-stimulated cells. The complex showed a considerable amount of CSF-1R complex-associated kinase activity. A detectable level of the complex was also present in untreated cells. PI-3 kinase in the multimeric complex displayed low lipid kinase activity despite the association with several proteins. The major pool of activated CSF-1R formed transient multimeric complexes with distinctly different tyrosine-phosphorylated proteins, which included STAT3 but also PI-3 kinase, Shc, SHP-1, and Grb2. A significant level of lipid kinase activity was detected in PI-3 kinase in the latter complexes. The different specific enzyme activities of PI-3 kinase in these complexes support the notion that the activity of PI-3 kinase is modulated by its association with CSF-1R and other associated cellular proteins. Specific structural proteins associated with the separate CSF-1R multimeric complexes upon CSF-1 stimulation and the presence of the distinct pools of the CSF-1R were dependent on the integrity of the microtubular network.
Assuntos
Macrófagos/metabolismo , Receptores de Fator Estimulador de Colônias/química , Transdução de Sinais , Animais , Linhagem Celular , Cromatografia por Troca Iônica , Citocalasina D/farmacologia , Ácidos Indolacéticos/farmacologia , Peptídeos e Proteínas de Sinalização Intracelular , Camundongos , Modelos Biológicos , Nocodazol/farmacologia , Fosfatidilinositol 3-Quinases/química , Fosforilação , Fosfotransferases/metabolismo , Testes de Precipitina , Conformação Proteica , Proteína Tirosina Fosfatase não Receptora Tipo 11 , Proteína Tirosina Fosfatase não Receptora Tipo 6 , Proteínas Tirosina Fosfatases/química , Frações Subcelulares , Fatores de Tempo , Tirosina/metabolismoRESUMO
Protein synthesis and secretion by post-natal sheep and calf retinal pigment epithelial (RPE) cells was investigated following labelling of choroidal pieces, isolated RPE cells and RPE cells in tissue culture with L-[U-14C] leucine. We show that RPE cells secrete a specific set of proteins that includes retinol binding protein (RBP) and transthyretin (TTR), which are both involved in retinol transport in blood. Using a two-chambered culture system we show that protein secretion by the post-natal RPE cells occurs predominantly across the apical pole of the cells, i.e., across the surface of the cells which, in vivo, faces the retina. In agreement with results of others using foetal RPE cells (Ong, D.E., Davis, J.T., O'Day, W.T. and Bok, D. (1994) Biochemistry 33, 1835-1842) we show that RBP and, to a lesser extent, TTR are also secreted predominantly across the apical pole of the cell. We have developed a cell culture model for the RPE that may be used as an in vitro model for studying transport across the blood-retinal barrier.
Assuntos
Epitélio Pigmentado Ocular/metabolismo , Pré-Albumina/biossíntese , Retina/metabolismo , Proteínas de Ligação ao Retinol/biossíntese , Animais , Transporte Biológico , Bovinos , Células Cultivadas , Corioide/metabolismo , Técnicas de Cultura , OvinosRESUMO
A murine Radiation leukemia virus-induced T cell lymphoma, 5C2, which is dependent on interleukin-2 (IL-2) for proliferation has been analyzed for interleukin-2 receptor (IL-2R) expression. Using fluorescence-activated cell sorter and electron microscopic analysis together with antibody specific for the known p55 chain of the murine IL-2R, no evidence has been obtained to suggest that these cells express detectable numbers of receptors with high affinity for IL-2. However, two different antibodies with specificity for the p55 chain of the IL-2R have been shown to inhibit 5C2 proliferation. An analysis of 125I-IL-2 binding has precluded a cell surface receptor density of greater than 80 molecules per cell. A temperature-dependent, nonspecific uptake of 125I-IL-2 has been described for 5C2. Uptake is saturated at 8.5 nM 125I-IL-2 with equilibrium being established within 60 min. When incubated at 37 degrees C in the presence of 400 pM 125I-IL-2, a maximum of approximately 2,000 molecules are internalized within 40 min. Uptake of other iodinated proteins by 5C2 was not observed. This property is unique to 5C2 and not to the control C6VL/1 cell line. Intracellular vesicles have also been found in 5C2 cells by electron microscopy which stain positively with gold-conjugated antibody specific for the p55 chain of the IL-2R. 5C2 appears to exhibit unique IL-2 regulatory characteristics.
Assuntos
Interleucina-2/metabolismo , Linfoma/metabolismo , Receptores Imunológicos/metabolismo , Animais , Divisão Celular , Linhagem Celular , Citometria de Fluxo , Cinética , Camundongos , Receptores de Interleucina-2 , Propriedades de Superfície , Linfócitos TRESUMO
The receptors for the constant region of immunoglobulin G (FcgammaR) are widely expressed on cells of hemopoietic lineage and plays an important role in host defense. We investigated the signaling pathways during FcgammaR-mediated phagocytosis in human monocyte-derived macrophages (MDMs) and examined the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on these events. FcgammaR-mediated phagocytosis resulted in enhanced tyrosine phosphorylation of a wide range of cellular proteins and activation of tyrosine kinases Hck, Syk, and Pyk2, as well as the multidomain adapter protein paxillin. Stimulation of MDMs with GM-CSF augmented FcgammaR-mediated phagocytosis and increased the levels of tyrosine phosphorylation in phagocytosing MDM cultures, indicating tyrosine kinase-mediated activation. GM-CSF treatment of MDMs without a phagocytic stimulus did not activate Syk, suggesting that GM-CSF may act either distally to Syk in the FcgammaR-mediated signaling cascade or on a parallel pathway activated by the FcgammaR. This study shows that early signaling events during FcgammaR-mediated phagocytosis in human MDMs involve activation of Syk, Hck, and paxillin. It also provides the first evidence for Pyk2 activation during phagocytosis and a baseline for further studies on the effect of GM-CSF on FcgammaR-mediated phagocytosis.
Assuntos
Fator Estimulador de Colônias de Granulócitos e Macrófagos/farmacologia , Macrófagos/imunologia , Fagocitose/efeitos dos fármacos , Proteínas Tirosina Quinases/fisiologia , Receptores de IgG/fisiologia , Técnicas de Cultura de Células , Precursores Enzimáticos/metabolismo , Precursores Enzimáticos/fisiologia , Quinase 2 de Adesão Focal , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Macrófagos/citologia , Monócitos/citologia , Fosforilação , Proteínas Tirosina Quinases/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas/fisiologia , Proteínas Proto-Oncogênicas c-hck , Quinase SykRESUMO
OBJECTIVE: HIV-1 infection impairs a number of macrophage effector functions, but the mechanism is unknown. We studied the role of HIV-1 Nef in modulating phagocytosis by human monocytes and monocyte-derived macrophages (MDM). DESIGN AND METHODS: Using a flow cytometric assay, phagocytosis of Mycobacterium avium complex (MAC) by monocytes in whole blood of Sydney Blood Bank Cohort (SBBC) members infected with a nef-deleted (Delta nef) strain of HIV-1 was compared with that of monocytes from uninfected or wild-type (WT) HIV-infected subjects. The specific impact of Nef on phagocytosis by MDM was determined by either infecting cells in vitro with Delta nef strains of HIV-1 or electroporating Nef into uninfected MDM. RESULTS: MAC phagocytic capacity of monocytes from SBBC members was equivalent to that of cells from uninfected individuals (P = 0.81); it was greater than that of cells from individuals infected with WT HIV-1 (P < 0.0001), irrespective of CD4 counts and HIV viral load. In contrast, in vitro infection of MDM with either Delta nef or WT strains of HIV-1 resulted in similar levels of HIV replication and equivalent impairment of phagocytosis via Fc gamma and complement receptors. Electroporation of Nef into MDM did not alter phagocytic capacity. CONCLUSIONS: This study provides evidence demonstrating the complex indirect effect of Nef on phagocytosis by peripheral blood monocytes (infrequently infected with HIV-1) in vivo. Conversely, the fact that MDM infected with either Delta nef or WT HIV-1 in vitro (high multiplicity of infection) show comparably impaired phagocytosis, indicates that HIV-1 infection of macrophages can directly impair function, independent of Nef.
Assuntos
Genes nef , Infecções por HIV/imunologia , HIV-1/genética , Macrófagos/imunologia , Monócitos/imunologia , Fagocitose , Contagem de Linfócito CD4 , Estudos de Coortes , Eletroporação , Citometria de Fluxo , Fluoresceína-5-Isotiocianato , Corantes Fluorescentes , Deleção de Genes , Infecções por HIV/virologia , HIV-1/imunologia , HIV-1/patogenicidade , Humanos , Immunoblotting , Técnicas In Vitro , Macrófagos/virologia , Monócitos/virologia , Carga ViralRESUMO
BACKGROUND: Previous studies of the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on HIV-1 replication in macrophages have had inconsistent results, variously reporting no effect, augmentation or inhibition of viral replication. OBJECTIVE: To investigate the regulation of HIV-1 in monocyte-derived macrophages (MDM) by GM-CSF in vitro. METHODS: The role of GM-CSF on HIV-1 replication was assessed as supernatant and intracellular p24 antigen concentrations and by HIV-1 DNA and mRNA production under different culture conditions. Expression of CD4 and CCR5 receptors was examined. The effect of GM-CSF with an E21R mutation, which binds only to the alpha-chain of GM-CSF receptor, was used as an additional control. RESULTS: GM-CSF consistently suppressed HIV-1 replication in human MDM in vitro, as assessed by supernatant and intracellular p24 antigen concentrations and HIV-1 gag mRNA expression. The inhibitory effect of GM-CSF on HIV-1 replication was observed regardless of HIV-1 strain, source of GM-CSF, stage of MDM maturation or timing of GM-CSF exposure in relation to HIV-1 infection. The effect was dose dependent and reversed by addition of a neutralizing monoclonal antibody (4D4). Flow cytometric analysis of surface expression of CD4 and CCR5 indicates that GM-CSF does not affect HIV-1 entry into MDM. Analysis of intracellular HIV-1 DNA and mRNA suggests that HIV-1 replication is inhibited at or before transcription. E21R GM-CSF had no effect on HIV-1 replication in MDM. CONCLUSIONS: GM-CSF regulates HIV-1 replication in MDM, inhibiting HIV-1 replication through binding to the beta-chain of the GM-CSF receptor.
Assuntos
Fator Estimulador de Colônias de Granulócitos/farmacologia , HIV-1/efeitos dos fármacos , Macrófagos/virologia , Monócitos/virologia , Replicação Viral/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Citometria de Fluxo , Genes gag , Fator Estimulador de Colônias de Granulócitos/imunologia , Fator Estimulador de Colônias de Granulócitos/fisiologia , Proteína do Núcleo p24 do HIV , HIV-1/fisiologia , Técnicas In Vitro , Macrófagos/efeitos dos fármacos , Monócitos/efeitos dos fármacos , RNA MensageiroRESUMO
There are differing views regarding the roles of phosphatidylinositol 3-kinases (PI3-kinases) and p70 S6 kinase (p70s6k) in growth factor-induced cellular responses. One approach that is widely employed to investigate these roles is to use the inhibitors, wortmannin and rapamycin, respectively. This approach is used here to study the responses in macrophages to colony stimulating factor-1 (CSF-1). Wortmannin (> or = 30 nM) and rapamycin (> or = 3 nM) both weakly inhibited CSF-1-stimulated DNA synthesis in murine bone marrow-derived macrophages (BMM), suggesting that there are PI3-kinase- and p70s6k-independent pathways required for the onset of S phase; interestingly the combination of the drugs gave dramatic suppression. Inhibition of DNA synthesis by rapamycin on the BMM was much less than that observed with the CSF-1-dependent cell line, BAC1.2F5. In BMM, wortmannin suppressed CSF-1-stimulated increase in p70s6k activity indicating that PI3-kinase activity may lie upstream. In contrast to some other growth factor/cell systems, no evidence was obtained using the inhibitors for the involvement of PI3-kinase or p70s6k in CSF-1-mediated induction of c-fos mRNA expression or Erk-1 activity; in addition, no evidence was found for an involvement in the CSF-1-mediated increase in cyclin D1 expression or STAT activation. The findings reinforce the need to study the signal transduction cascades relevant to each individual growth factor and preferably not in cell lines.
Assuntos
Androstadienos/farmacologia , Inibidores de Cisteína Proteinase/farmacologia , Inibidores Enzimáticos/farmacologia , Fator Estimulador de Colônias de Macrófagos/farmacologia , Macrófagos/efeitos dos fármacos , Proteínas Quinases Ativadas por Mitógeno , Inibidores de Fosfoinositídeo-3 Quinase , Polienos/farmacologia , Proteínas Quinases S6 Ribossômicas/antagonistas & inibidores , Animais , Células da Medula Óssea/efeitos dos fármacos , Proteínas Quinases Dependentes de Cálcio-Calmodulina/genética , Linhagem Celular , Ciclina D1/biossíntese , DNA/biossíntese , Indução Enzimática/efeitos dos fármacos , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Genes fos/genética , Macrófagos/metabolismo , Camundongos , Proteína Quinase 3 Ativada por Mitógeno , Fosfatidilinositol 3-Quinases/biossíntese , RNA Mensageiro/biossíntese , Proteínas Quinases S6 Ribossômicas/biossíntese , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Sirolimo , WortmaninaRESUMO
A relatively simple method has been used to clone the gene coding for the respiratory NADH dehydrogenase (NADH-ubiquinone oxidoreductase) of Escherichia coli from unfractionated chromosomal DNA. The restriction endonucleases EcoRI, BamI and HindIII were used to construct three hybrid plasmid pools from total E. coli DNA and the amplifiable plasmids pSF2124 and pGM706. Three different restriction endonucleases were used to increase the chances of cloning the ndh gene intact. Mobilization by the plasmid F was used to transfer the hybrid plasmids into ndh mutants and selection was made for Apr and complementation of ndh. DNA fragments complementing ndh were isolated from both the EcoRI and HindIII hybrid plasmid pools. The strain carrying the hybrid plasmid constructed with EcoRI produced about 8--10 times the normal level of the respiratory NADH dehydrogenase in the cytoplasmic membrane. Treating the cells with chloramphenicol to increase the plasmid copy number allowed the level of NADH dehydrogenase in the membrane to be increased to 50--60 times the level in the wild type. The results indicate the potential of gene cloning for the specific amplification of particular proteins prior to their purification.
Assuntos
Replicação do DNA , DNA Recombinante , NADH NADPH Oxirredutases/genética , Enzimas de Restrição do DNA/metabolismo , DNA Bacteriano/genética , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Escherichia coli/enzimologia , Genes , Métodos , PlasmídeosRESUMO
Calponin (4.1-5.9 microM, pig stomach) inhibited maximal shortening velocity (Vmax) by 20-25% with only minor influence on force in skinned smooth muscle from guinea-pig taenia coli activated at different Ca2+ levels and with thiophosphorylation. Similar results were obtained with a fragment of the N-terminal 1-228 amino acids engineered using a mouse cDNA construct (5.4 microM). Both the native calponin and the fragment inhibited actin filament sliding in a graded manner in an in vitro motility assay. We conclude that calponin influences the kinetics of the actin-myosin interaction in the organised smooth muscle contractile system and that engineered fragments of calponin can be used to probe its action in muscle fibres. The effects can be due to an introduction of an internal load during filament sliding, possibly by decreasing the detachment rates and increasing the cross-bridge time spent in the attached state.
Assuntos
Proteínas de Ligação ao Cálcio/farmacologia , Colo/fisiologia , Contração Isométrica/efeitos dos fármacos , Fibras Musculares Esqueléticas/fisiologia , Músculo Liso/fisiologia , Actinas/metabolismo , Animais , Cálcio/metabolismo , Colo/efeitos dos fármacos , Relação Dose-Resposta a Droga , Técnicas In Vitro , Proteínas dos Microfilamentos , Fibras Musculares Esqueléticas/efeitos dos fármacos , Proteínas Musculares/farmacologia , Músculo Esquelético/fisiologia , Músculo Liso/efeitos dos fármacos , Miosinas/metabolismo , Fragmentos de Peptídeos/farmacologia , Estômago , Suínos , Fatores de Tempo , CalponinasRESUMO
A large proportion of neurons die during normal development of the nervous system via an active process known as apoptosis. We counted the total number of neurons and apoptotic neurons in the superior cervical ganglion of the GH Wistar rat strain, which possesses a neurotrophic deficit leading to excessive perinatal cell death, and in its normal counterpart (N) by using the optical disector method to quantify the extent of apoptosis during postnatal development. Total neuron numbers fell between postnatal days 3 and 14 by 10 and 40% in N and GH, respectively. In GH ganglia, 1.5% of neurons were apoptotic at any given time, as determined by the presence of condensed chromatin clumps. Some types of cell death have been associated with expression of the immediate-early genes c-fos and c-jun. Therefore, we used histological and immunocytochemical techniques to characterise individual neurons and to detect the products of these immediate-early genes during developmental cell death. All apoptotic cells were immunopositive for c-jun protein, whereas no c-jun protein was detected in nonapoptotic cells. Conversely, members of the fos family of transcription factors were detected in the nucleus of 60% of nonapoptotic cells but in only a minor proportion of cells undergoing apoptosis. These results indicate that c-jun occurs in neurons that are committed to die. This is the first situation in which the presence of jun protein has been correlated with normal programmed cell death in individual apoptotic neurons.
Assuntos
Apoptose/fisiologia , Proteínas do Tecido Nervoso/análise , Neurônios/metabolismo , Proteínas Proto-Oncogênicas c-fos/análise , Proteínas Proto-Oncogênicas c-jun/análise , Gânglio Cervical Superior/fisiologia , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Bisbenzimidazol , Corantes Fluorescentes , Técnicas Imunoenzimáticas , Fatores de Crescimento Neural/farmacologia , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neuropeptídeo Y/análise , Ratos , Ratos Wistar , Gânglio Cervical Superior/citologia , Gânglio Cervical Superior/efeitos dos fármacosRESUMO
This report compares the relative levels of messenger RNA species coding for plasma proteins in rat visceral yolk sac and fetal liver from 12.5 days to 21.5 days gestation. Transthyretin, retinol-binding protein, transferrin and alpha 1-fetoprotein mRNAs were detected in both tissues, although relative levels were much higher in the yolk sac compared to fetal liver, in early gestation. Messenger RNA coding for the positive acute phase proteins thiostatin, fibrinogen, alpha 2-macroglobulin and alpha 1-antitrypsin were detected at a low but significant level in yolk sac, while the levels in fetal liver steadily increased from 16.5 days gestation and, with the exception of alpha 1-antitrypsin, reached levels higher than those found in adult liver just prior to birth. Albumin, inter-alpha 1-trypsin inhibitor, alpha 1-acid glycoprotein, haptoglobin, vitamin D-binding protein and ceruloplasmin messenger RNA levels were either very low or undetectable in yolk sac and fetal liver. Secretion of proteins by yolk sac endoderm occurred largely across the basolateral surface, i.e. towards the fetal compartment. These data support the hypothesis that one function of the yolk sac in the rat is the synthesis and secretion of a select group of plasma proteins to maintain homeostasis in the fetal compartment in the period before the fetal liver has matured sufficiently to carry out this function.
Assuntos
Proteínas Sanguíneas/biossíntese , Feto/metabolismo , Hormônios Tireóideos , Saco Vitelino/metabolismo , Albuminas/biossíntese , Albuminas/metabolismo , alfa-Globulinas/biossíntese , alfa-Globulinas/metabolismo , Aminoácidos/metabolismo , Animais , Proteínas Sanguíneas/metabolismo , Northern Blotting , Proteínas de Transporte , Ceruloplasmina/biossíntese , Ceruloplasmina/metabolismo , Fibrinogênio/biossíntese , Fibrinogênio/metabolismo , Haptoglobinas/biossíntese , Haptoglobinas/metabolismo , Fígado/metabolismo , Proteínas de Membrana , Orosomucoide/biossíntese , Orosomucoide/metabolismo , Pré-Albumina/biossíntese , Pré-Albumina/metabolismo , RNA Mensageiro/análise , Ratos , Proteínas de Ligação ao Retinol/biossíntese , Proteínas de Ligação ao Retinol/metabolismo , Proteínas Plasmáticas de Ligação ao Retinol , Transferrina/biossíntese , Transferrina/metabolismo , Proteína de Ligação a Vitamina D/biossíntese , Proteína de Ligação a Vitamina D/metabolismo , alfa 1-Antitripsina/biossíntese , alfa 1-Antitripsina/metabolismo , alfa-Fetoproteínas/biossíntese , alfa-Fetoproteínas/metabolismo , alfa-Macroglobulinas/biossíntese , alfa-Macroglobulinas/metabolismo , Proteínas de Ligação a Hormônio da TireoideRESUMO
The uptake of transferrin by the rat visceral yolk sac membranes, and the fate of this protein, were measured in a two-chambered system which allowed access to both surfaces of these membranes, i.e. that facing the maternal compartment and that facing the fetal compartment. 125I-labeled transferrin was internalized by the maternal surface of the visceral yolk sac but not by the fetal surface. Following internalization, this transferrin was degraded and the amino acids were secreted exclusively towards the fetal compartment. Transcytosis of intact transferrin was not detected in either direction. These results suggest that transport across the rat visceral yolk sac bound to maternally derived transferrin is not a major mechanism of iron transport in vivo. These results support a role for the visceral yolk sac in fetal metabolism, or supplying the fetus with amino acids derived from degradation of specific maternal plasma proteins, in this case, transferrin.
Assuntos
Troca Materno-Fetal , Transferrina/metabolismo , Saco Vitelino/metabolismo , Animais , Feminino , Hidrólise , Gravidez , RatosRESUMO
Antibodies (Abs) that mediate antibody-dependent cellular cytotoxicity (ADCC) activity against HIV-1 are of major interest. A widely used method to measure ADCC Abs is the rapid and fluorometric antibody-dependent cellular cytotoxicity (RFADCC) assay. Antibody-dependent killing of a labelled target cell line by PBMC is assessed by loss of intracellular CFSE but retention of membrane dye PKH26 (CFSE-PKH26+). Cells of this phenotype are assumed to be derived from CFSE+PKH26+ target cells killed by NK cells. We assessed the effector cells that mediate ADCC in this assay. Backgating analysis and phenotyping of CFSE-PKH26+ revealed that the RFADCC assay's readout mainly represents CD3-CD14+ monocytes taking up the PKH26 dye. This was confirmed for 53 HIV+plasma-purified IgG samples when co-cultured with PBMC from three separate healthy donors. Emergence of the CFSE-PKH26+ monocyte population was observed upon co-culture of targets with purified monocytes but not with purified NK cells. Image flow cytometry and microscopy showed a monocyte-specific interaction with target cells without typical morphological changes associated with phagocytosis, suggesting a monocyte-mediated ADCC process. We conclude that the RFADCC assay primarily reflects Ab-mediated monocyte function. Further studies on the immunological importance of HIV-specific monocyte-mediated ADCC are warranted.
Assuntos
Citotoxicidade Celular Dependente de Anticorpos/imunologia , HIV/imunologia , Células Matadoras Naturais/imunologia , Monócitos/imunologia , Complexo CD3/imunologia , Complexo CD3/metabolismo , Linhagem Celular , Células Cultivadas , Técnicas de Cocultura , Citometria de Fluxo , Fluoresceínas/química , Corantes Fluorescentes/química , Humanos , Células Matadoras Naturais/química , Células Matadoras Naturais/metabolismo , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/metabolismo , Receptores de Lipopolissacarídeos/imunologia , Receptores de Lipopolissacarídeos/metabolismo , Microscopia de Fluorescência , Monócitos/citologia , Monócitos/metabolismo , Compostos Orgânicos/química , Fagocitose/imunologia , Análise de Célula Única/métodos , Succinimidas/químicaRESUMO
We have studied the temperature dependence of isometric force, rate of force development and maximal shortening velocity (Vmax) in skinned guinea-pig taenia coli smooth muscle. To eliminate the influence of temperature on activation mechanisms, maximally thiophosphorylated preparations were used. Isometric force in the range 2-35 degrees C was maximal at 22 degrees C with a decrease of 25% at 2 degrees C and 10% at 35 degrees C. Rate of tension development from rigor after photolytic release of ATP increased four-fold between 5 degrees C and 30 degrees C. Vmax increased with a Q10 of about 2 (1.6, range 5-15 degrees C, and 2.2, range 22-30 degrees C). The temperature dependence of the rate of tension development indicates rate-limitation by transitions into force-generating states or by the hydrolysis reaction. The temperature dependence of Vmax reflects effects of temperature on reactions (e.g. the ADP-release) associated with cross-bridge detachment. The small temperature dependence of steady-state force in smooth compared with skeletal muscle suggests differences in the cross-bridge reactions controlling the number of attached force-generating states in the two muscle types.
Assuntos
Colo/fisiologia , Contração Isométrica/fisiologia , Músculo Liso/fisiologia , Trifosfato de Adenosina/análogos & derivados , Trifosfato de Adenosina/metabolismo , Animais , Cobaias , Técnicas In Vitro , Cinética , Fibras Musculares Esqueléticas/fisiologia , Fotólise , Tempo de Reação , Estresse Mecânico , TemperaturaRESUMO
The proteins secreted by the choroid plexus throughout rat brain development were analyzed by two-dimensional polyacrylamide gel electrophoresis following biosynthetic labeling of choroid plexus pieces with [14C]leucine in vitro. Approximately 20 major protein species were resolved which, with the exception of transferrin, transthyretin, and alpha 2-macroglobulin, appear to be unrelated to proteins found in high concentrations in plasma. Several patterns of developmental regulation were observed. At least two of the proteins were synthesized and secreted at high levels only by fetal choroid plexus, whereas the secretion of several other proteins including transferrin and proteins comigrating with cystatin C and alpha 2-macroglobulin increased only after birth. The levels of mRNA coding for transferrin, ceruloplasmin, cystatin C, alpha 2-macroglobulin, beta 2-microglobulin, and transthyretin were measured in the brain during development by dot hybridization and northern gel analysis. No mRNA was detected coding for the proteins alpha-fetoprotein, alpha 1-antitrypsin, haptoglobin, and thiostatin in the brain at any stage. For those proteins, which are produced in other parts of the brain as well as by the choroid plexus, the changes in their corresponding mRNA levels measured in whole brain paralleled the changes in their secretion by the choroid plexus. The results presented in this paper show that the choroid plexus is active in protein secretion at all stages studied. The changing pattern of protein secretion by the choroid plexus, combined with its early development compared with other tissues in the brain, suggests that it is active in providing the appropriate extracellular environment for the growth and differentiation of the brain.
Assuntos
Encéfalo/crescimento & desenvolvimento , Plexo Corióideo/crescimento & desenvolvimento , Cistatinas , Regulação da Expressão Gênica , Proteínas do Tecido Nervoso/genética , Envelhecimento/metabolismo , Animais , Proteínas Sanguíneas/genética , Proteínas Sanguíneas/metabolismo , Encéfalo/embriologia , Encéfalo/metabolismo , Ceruloplasmina/genética , Ceruloplasmina/metabolismo , Plexo Corióideo/embriologia , Plexo Corióideo/metabolismo , Cistatina C , Sondas de DNA , Eletroforese em Gel de Poliacrilamida , Focalização Isoelétrica , Proteínas do Tecido Nervoso/metabolismo , Hibridização de Ácido Nucleico , Pré-Albumina/genética , Pré-Albumina/metabolismo , Proteínas/genética , Proteínas/metabolismo , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos BUF , Transferrina/genética , Transferrina/metabolismo , alfa-Macroglobulinas/genética , alfa-Macroglobulinas/metabolismo , Microglobulina beta-2/genética , Microglobulina beta-2/metabolismoRESUMO
When the enzymatically generated intermediate 2-carboxy-3-keto-D-arabinitol-1,5-bisphosphate (II) was used as a substrate with fresh enzyme, 70% reacted to produce 3-phosphoglycerate (3PGA). When a reaction mixture of enzyme plus [1-32P]ribulose 1,5-bisphosphate (RuBP) was quenched in the steady state with the tightly bound inhibitor 2-carboxyarabinitol-1,5-bisphosphate, 30% of the enzyme-bound species was released as 3PGA and 70% as RuBP. The major source for this partition was the ternary substrates Michaelis complex. The level of carboxylated intermediate in the steady state was determined to be 8% of active sites under the conditions of substrate saturation. No burst was seen in the appearance of product when 6.5 eq of [1-32P]RuBP was mixed with enzyme plus saturating CO2 and the reaction followed in the steady state. From these data plus the steady-state Vmax and Km of RuBP it is possible to derive the five bulk rate constants represented in the scheme ECO2 + RuBP in equilibrium ERuBPCO2 in equilibrium E X II----E + 2(3PGA).
Assuntos
Rhodospirillum rubrum/enzimologia , Ribulose-Bifosfato Carboxilase/metabolismo , Ácidos Glicéricos/metabolismo , Glicina/análogos & derivados , Glicina/metabolismo , Cinética , MatemáticaRESUMO
The central pathogenic feature of AIDS is the dramatic loss of CD4+ lymphocytes. Despite more than a decade of intense research, the exact mechanism by which HIV causes this is still not understood. A major model for T cell depletion, proposed originally by Ameison and Capron in a report published in 1991, is that HIV sensitizes CD4+ T cells for activation-induced apoptosis. The apoptotic model of T cell depletion is discussed, and experiments that address the questions of whether apoptosis is restricted to infected cells or 'bystander' T cells, and whether T cell apoptosis requires participation of separate HIV-infected haematopoietic cell populations, are reviewed.
Assuntos
Apoptose/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , HIV/patogenicidade , Contagem de Linfócito CD4 , Células Cultivadas , Proteína Ligante Fas , HIV/imunologia , Infecções por HIV/imunologia , Infecções por HIV/patologia , Humanos , Linfopenia/etiologia , Linfopenia/imunologia , Linfopenia/patologia , Macrófagos/imunologia , Glicoproteínas de Membrana/metabolismo , Monócitos/imunologia , Receptor fas/metabolismoRESUMO
We have investigated the effects of vanadate (Vi) on force generation by, and shortening of, chemically skinned smooth muscle preparations from guinea-pig taenia coli at 22 degrees C. A method, using phosphatase inhibitors, was introduced to obtain stable, long-lasting contractions in thiophosphorylated preparations. Vi (10-1000 microM) dose-dependently inhibited active force, to about 20% of its maximum level. At a higher temperature (30 degrees C), the rate of inhibition was faster but the extent of inhibition was less. The rate of contraction following photolytic release of ATP to fibres in rigor was not affected by Vi (30 microM). The maximal shortening velocity (Vmax) was inhibited in a similar manner as active force by Vi (30 microM). In conclusion, the results suggest that Vi interacts with a force-generating actomyosin-ADP (AMADP) state reached after phosphate release. The rate of inhibition of smooth muscle contraction was markedly lower than in skeletal muscle, suggesting differences either in properties of the Vi-bound states or, more likely, in the concentration of AMADP states capable of binding Vi. This suggests that the long duty cycle in smooth muscle is not associated with a higher relative population of AMADP states reached immediately after Pi release, but rather by an increase in the population of subsequent force-generating cross-bridge states. The Vi-bound cross-bridges introduce an internal load to shortening, possibly acting in a similar manner as cross-bridge states introduced at low levels of activation.
Assuntos
Contração Muscular/efeitos dos fármacos , Músculo Liso/fisiologia , Vanadatos/farmacologia , Animais , Relação Dose-Resposta a Droga , Cobaias , Técnicas In Vitro , Cinética , Músculo Liso/efeitos dos fármacos , Fotólise , Vanadatos/administração & dosagemRESUMO
Phospholipase D activity was measured in murine bone marrow-derived macrophages (BMM) treated with either colony stimulating factor-1 (CSF-1) or phorbol myristyl acetate (PMA) by measuring formation of phosphatidylbutanol (PtBut) in cells preloaded with n-butanol. Addition of 10(-7) M PMA for 15 min stimulated the amount of PtBut formed in growth arrested cells by 3-4 fold whereas no stimulation was observed with 5000 units mL-1 CSF-1 for 0.5, 2 or 15 min. Protein kinase C activity was determined in growth-arrested BMM by phosphorylation of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS). PMA stimulation for 5 min increased protein kinase C activity 5-6 fold whereas CSF-1 treatment for 5 min or 15 min did not. Contrary to earlier reports, CSF-1 did not stimulate diradyl glycerol formation in BMM. These results show that stimulation of protein kinase C and the activation of phospholipase D are not involved in the early events of CSF-1-stimulated signal transduction pathways in BMM.