RESUMO
As a common disease, osteonecrosis attracts more and more attention. In this paper, we investigated the relationship between the alterations of endothelial-derived and platelet-derived microparticles and the changes of coagulation and inflammation in the steroid-induced avascular osteonecrosis of femoral head using the rabbit model. We also explored the possible mechanism of the membrane particles associated with the development of the rabbit femoral head ischemic necrosis. With a 28-day continuous observation, the level of membrane microparticles was significantly heightened after methylprednisolone treatment. The coagulating and inflammatory factors also tended to increase. The data demonstrated that the levels of membrane microparticles had significantly individual differences, which meant the increased levels of membrane microparticles may be related to hypercoagulability, thrombosis, and inflammation in microcirculation and played an important role in steroid-induced osteonecrosis. It will be very useful and helpful to guide clinical trials.
Assuntos
Micropartículas Derivadas de Células/efeitos dos fármacos , Modelos Animais de Doenças , Necrose da Cabeça do Fêmur/induzido quimicamente , Metilprednisolona/efeitos adversos , Metilprednisolona/farmacologia , Osteonecrose/induzido quimicamente , Osteonecrose/patologia , Animais , Feminino , Citometria de Fluxo , Injeções Intramusculares , Masculino , CoelhosRESUMO
OBJECTIVE: To investigate the effects of EMPs on HUVECs permeability in vitro, then evaluate the effect on the Avascular Necrosis of Femoral Head. METHODS: Incubate HUVECs with EMPs which is released by HUVECs in vitro, then estimate the endothelial permeability. RESULTS: Flow cytometry analysis showed that EMPs were released at maximum concentration(7.66 × 10(4)/ml) after 24 hours stimulated by 10 mmol DEX And 7.66 × 10(4), 7.66 × 10(3)/ml concentration (moderate and high concentrations) of EMPs caused injury to the permeability of HUVECs (P values are 0.002 and 0.031 respectively, < 0.05), while 7.66 × 10(2)/ml concentration (low concentration) of EMPs didn't (P = 0.425 > 0.05). CONCLUSION: Dexamethasone can induce HUVECs to release EMPs in vitro. EMPs caused collapse of connections of HUVECs, and increase the HUVECs permeability, decrease vascular stability, finally lead to Avascular Necrosis of Femoral Head.