Photo-Stimulated Zn-based Batteries: Progress, Challenges, and Perspectives.

Solar energy, as a renewable energy source, dominates the vast majority of human energy, which can be harvested and converted by photovoltaic solar cells. However, the intermittent availability of solar energy restricts the actual utilization circumstances of solar cells. Integrating photo-responsive electrodes into an energy storage device emerges as a dependable and executable strategy, fostering the creation of photo-stimulated batteries that seamlessly amalgamate the process of solar energy collection, conversion, and storage in one system. Endowed by virtues such as cost-effectiveness, facile manufacturing, safety, and environmental friendliness, photo-stimulated Zn-based batteries have attracted considerable attention. The progress report furnishes a brief overview, summarizing various photo-stimulated Zn-based batteries. Their configurations, operational principles, advancements, and the intricate engineering of photoelectrode designs are introduced, respectively. Through rigorous architectural design, photo-stimulated Zn-based batteries exhibit the ability to initiate charging by saving electricity usage, and in certain instances, even without the need for external electrical grids under illumination. Furthermore, the compensation of solar energy can be explored to improve the output electric energy. At last, opportunities and challenges toward photo-stimulated Zn-based batteries in the process of development are proposed and discussed in the hope of expanding their application scenarios and accelerating the commercialization progress.

Fabrication of Single-Crystal Violet Phosphorus Flakes For Ultrasensitive Photodetection.

Violet phosphorus (VP) has attracted a lot of attention for its unique physicochemical properties and emerging potential in photoelectronic applications. Although VP has a van der Waals (vdW) structure similar to that of other 2D semiconductors, direct synthesis of VP on a substrate is still challenging. Moreover, optoelectronic devices composed of transfer-free VP flakes have not been demonstrated. Herein, a bismuth-assisted vapor phase transport technique is designed to grow uniform single-crystal VP flakes on the SiO2 /Si substrate directly. The size of the crystalline VP flakes is an order of magnitude larger than that of previous liquid-exfoliated samples. The photodetector fabricated with the VP flakes shows a high responsivity of 12.5 A W-1 and response/recovery time of 3.82/3.03 ms upon exposure to 532 nm light. Furthermore, the photodetector shows a small dark current (<1 pA) that is beneficial to high-sensitivity photodetection. As a result, the detectivity is 1.38 × 1013 Jones that is comparable with that of the vdW p-n heterojunction detector. The results reveal the great potential of VP in optoelectronic devices as well as the CVT technique for the growth of single-crystal semiconductor thin films.

RNA m6A reader IGF2BP3 promotes metastasis of triple-negative breast cancer via SLIT2 repression.

Triple-negative breast cancer (TNBC) is a group of fatal malignancies characterized by high metastatic capacity, the underlying mechanisms of which remain largely elusive. We have found here that insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) is highly expressed in TNBC and correlates clinically with distant metastasis-free survival of TNBC patients. IGF2BP3 promotes the migration and invasion capabilities of TNBC cells dependent upon cellular RNA N6-methyladenosine (m6A) modification. Mechanistically, IGF2BP3 binds to and destabilizes m6A-methylated mRNA of the extracellular matrix glycoprotein, SLIT2, impairs its downstream signaling via the cognate receptor ROBO1, and consequently triggers the activation of canonical PI3K/AKT and MEK/ERK pathways. The IGF2BP3/SLIT2 axis is critically involved in the regulation of TNBC metastasis in vivo. These findings shed light into the regulatory network of distant metastasis of breast cancer and provide rationale for targeting the m6A machinery in the treatment of TNBC.

Effect of inhalation aromatherapy on physical and psychological problems in cancer patients: Systematic review and Meta-analysis.

PURPOSE: This systematic review and meta-analysis evaluated the effects of inhalation aromatherapy on physical and psychological problems in cancer patients. METHODS: We searched relevant randomized controlled trials and quasi-randomized controlled trials in PubMed, Cochrane Library, Web of Science, Embase, CINHAL, CNKI, CBM, Wan Fang, and VIP databases, and then evaluated the quality and extracted data from these studies. The publication date was from the establishment of each database until May 2021. RESULTS: We ultimately included 16 publications that examined 636 patients and 636 controls. Inhalation of aromatherapy had no effect on pain and depression; mixed results on nausea and vomiting; reduced sleep disorders and anxiety; and improved quality of life. CONCLUSIONS: Although inhalation aromatherapy may benefit cancer patients, more high-quality evidence is needed to support its clinical usefulness.

Ultrathin two-dimensional Fe-doped cobaltous oxide as a piezoelectric enhancement mechanism in quartz crystal tuning fork (QCTF) photodetectors.

An innovative ultrathin two-dimensional (2D) Fe-doped cobaltous oxide (Fe-CoO) coated quartz crystal tuning fork (QCTF) was introduced for the purpose of developing a low-cost photoelectric detector with a simple configuration. The enhancement mechanism of the piezoelectric signal in the ultrathin 2D Fe-CoO-coated QCTF detector is assumed to be the synergetic photocarrier transfer and photothermal effect of ultrathin 2D Fe-CoO. The ultrathin 2D nanosheet structure of Fe-CoO with a large specific surface area can efficiently absorb and convert light into heat in the QCTF, and the photocarrier transfer from the Fe-CoO nanosheet to the electrode of the QCTF contributes to the enhancement in electricity given the shortened diffusion distance of carriers to the surfaces of the 2D nanosheet. Finite element modeling was adopted to simulate the thermoelastic expansion and mechanical resonance of the QCTF with 2D Fe-CoO coating to support experimental results and analyses. Moreover, the effects of 2D Fe-CoO on the performance of QCTF-based photoelectric detectors were investigated. This Letter demonstrates that ultrathin 2D materials have great potential in applications such as costly and tiny QCTF detectors, light sensing, biomedical imaging, and spectroscopy.

Factors that affect the duration of wearing disposable personal protective equipment by healthcare professionals in Wuhan during treatment of COVID-19 patients: An epidemiological study.

The purpose of this study of healthcare workers who cared for COVID-19 patients was to identify factors that affected the duration of wearing personal protective equipment (PPE). The results of this study will provide initial guidance to practicing clinicians and a foundation for further research on this topic. This cross-sectional study examined 139 frontline healthcare professionals who worked at a single hospital in Wuhan, China, from March 16 to April 1, 2020. General and demographic data, physical and mental status, use of personal protective equipment, type of hospital work, and duration of wearing personal protective equipment were recorded. The mean duration of wearing personal protective equipment was 194.17 min (standard deviation: 3.71). Multiple linear regression analysis indicated that the duration of wearing personal protective equipment was significantly associated with the presence of a chronic disease, working hours when feeling discomfort, lack of patient cooperation and subsequent psychological pressure, prolonged continuous wearing of personal protective equipment, feeling anxious about physical strength, and the presence of fatigue when wearing personal protective equipment. These factors should be considered by practicing healthcare professionals and in future studies that examine the optimal duration of wearing personal protective equipment.

Antitumor activity of a novel Aurora A/B kinases inhibitor TY-011 against gastric cancer by inducing DNA damage.

TY-011, a novel Aurora A/B kinases inhibitor, was found in our previous study to exhibit prominent inhibitory effects on growth of gastric cancer, both in vitro and in vivo. To clarify the mechanisms of TY-011 in inhibiting proliferation of gastric cancer cells, the effects of TY-011 on mitosis, cell cycle, apoptosis and cellular DNA were checked in the present study. Our results showed that TY-011 treatment induced aberrant mitosis, G2/M phase arrest and apoptosis. Importantly, TY-011 induced evident DNA damage in MGC-803 and MKN-45 human gastric cancer cells, which was further characterized as DNA double-strand break. Furthermore, cells treated with TY-011 appeared to generate multiple spindle fibers emanating from several spindle poles, leading to poly-merotelic kinetochore. These results suggested that TY-011 induced abnormal microtubule-kinetochores attachment and thus DNA damage, apoptosis and finally inhibition of cell proliferation of human gastric cancer cells.

Au@SiO2@CuInS2-ZnS/Anti-AFP fluorescent probe improves HCC cell labeling.

BACKGROUND: Clear tumor imaging is essential to the resection of hepatocellular carcinoma (HCC). This study aimed to create a novel biological probe to improve the HCC imaging. METHODS: Au nano-flower particles and CuInS2-ZnS core-shell quantum dots were synthesized by hydrothermal method. Au was coated with porous SiO2 and combined with anti-AFP antibody. HCC cell line HepG2 was used to evaluate the targeting efficacy of the probe, while flow cytometry and MTT assay were used to detect the cytotoxicity and bio-compatibility of the probe. Probes were subcutaneously injected to nude mice to explore light intensity and tissue penetration. RESULTS: The fluorescence stability of the probe was maintained 100% for 24 h, and the brightness value was 4 times stronger than that of the corresponding CuInS2-ZnS quantum dot. In the targeting experiment, the labeled HepG2 emitted yellow fluorescence. In the cytotoxicity experiments, MTT and flow cytometry results showed that the bio-compatibility of the probe was fine, the inhibition rate of HepG2 cell with 60% Cu-QDs/Anti-AFP probe and Au-QDs/Anti-AFP probe solution for 48 h were significantly different (86.3%±7.0% vs. 4.9%±1.3%, t = 19.745, P<0.05), and the apoptosis rates were 83.3%±5.1% vs. 4.4%±0.8% (P<0.001). In the animal experiment, the luminescence of the novel probe can penetrate the abdominal tissues of a mouse, stronger than that of CuInS2-ZnS quantum dot. CONCLUSIONS: The Au@SiO2@CuInS2-ZnS/Anti-AFP probe can targetedly recognize and label HepG2 cells with good bio-compatibility and no toxicity, and the strong tissue penetrability of luminescence may be helpful to surgeons.

Facile fabrication and configuration design of Co3O4 porous acicular nanorod arrays on Ni foam for supercapacitors.

The configuration of electrode materials is of great significance to the performance of supercapacitors (SCs) because of its direct effects on specific surface area and electron transfer path. Given this, herein, a series of Co3O4 hierarchical configurations composed of porous acicular nanorods are designedly synthesized on Ni foam with in-site self-organization method depending on the addition of NH4F. In the absence of NH4F, Co3O4 nanorods self-assemble into porous urchin-like structure (PULS), while the introduction of NH4F can induce the vertical growth of Co3O4 acicular nanorods, forming porous acicular nanorod arrays (PANRAs). By simply tuning the concentration of NH4F, the Co3O4 PANRAs with different specific surface area can be obtained. As expected, Co3O4 PANRAs electrode for SCs (using 1 mmol of NH4F) exhibits high specific capacitance (1486 F g-1 at 1 A g-1) and excellent cycling stability (98.8% retention after 5000 continuous charge-discharge cycles), which are better than those of Co3O4 PULS electrode (658.2 F g-1 at 1 A g-1, 90.4%). Corresponding solid-state symmetric SC achieves a high energy density of 48.63 Wh kg-1 at power density of 600 W kg-1. Such superior performance is attributed to fast charge transfer kinetics, facile electron transport and ions diffusion rate resulting from porous array structure, indicating the importance of configuration design of electrode materials for high performance SCs.

Cd-free Cu-Zn-In-S/ZnS quantum dots@SiO2 multiple cores nanostructure: preparation and application for white LEDs.

The work reports the fabrication of Cu doped Zn-In-S (CZIS) alloy quantum dots (QDs) using dodecanethiol and oleic acid as stabilizing ligands. With the increase of doped Cu element, the photoluminescence (PL) peak is monotonically red shifted. After coating ZnS shell, the PL quantum yield of CZIS QDs can reach 78%. Using reverse micelle microemulsion method, CZIS/ZnS QDs@SiO2 multi-core nanospheres were synthesized to improve the colloidal stability and avoid the aggregation of QDs. The obtained multi-core nanospheres were dispersed in curing adhesive, and applied as a color conversion layer in down converted light-emitting diodes. After encapsulation in curing adhesive, the newly designed LEDs show artifically regulated color coordinates with varying the weight ratio of green QDs and red QDs, and the concentrations of these two types of QDs. Moreover, natural white and warm white LEDs with correlated color temperature of 5287, 6732, 2731, and 3309 K can be achieved, which indicates that CZIS/ZnS QDs@SiO2 nanostructures are promising color conversion layer material for solid-state lighting application.

Transcriptome Sequencing of Dianthus spiculifolius and Analysis of the Genes Involved in Responses to Combined Cold and Drought Stress.

Dianthus spiculifolius, a perennial herbaceous flower and a member of the Caryophyllaceae family, has strong resistance to cold and drought stresses. To explore the transcriptional responses of D. spiculifolius to individual and combined stresses, we performed transcriptome sequencing of seedlings under normal conditions or subjected to cold treatment (CT), simulated drought treatment (DT), or their combination (CTDT). After de novo assembly of the obtained reads, 112,015 unigenes were generated. Analysis of differentially expressed genes (DEGs) showed that 2026, 940, and 2346 genes were up-regulated and 1468, 707, and 1759 were down-regulated in CT, DT, and CTDT samples, respectively. Among all the DEGs, 182 up-regulated and 116 down-regulated genes were identified in all the treatment groups. Analysis of metabolic pathways and regulatory networks associated with the DEGs revealed overlaps and cross-talk between cold and drought stress response pathways. The expression profiles of the selected DEGs in CT, DT, and CTDT samples were characterized and confirmed by quantitative RT-PCR. These DEGs and metabolic pathways may play important roles in the response of D. spiculifolius to the combined stress. Functional characterization of these genes and pathways will provide new targets for enhancement of plant stress tolerance through genetic manipulation.

Bio-compatibility and cytotoxicity studies of water-soluble CuInS2-ZnS-AFP fluorescence probe in liver cancer cells.

BACKGROUND: The oncogenesis of hepatocellular carcinoma (HCC) is not clear. The current methods of the pertinent studies are not precise and sensitive. The present study was to use liver cancer cell line to explore the bio-compatibility and cytotoxicity of ternary quantum dots (QDs) probe and to evaluate the possible application of QDs in HCC. METHODS: CuInS2-ZnS-AFP fluorescence probe was designed and synthesized to label the liver cancer cell HepG2. The cytotoxicity of CuInS2-ZnS-AFP probe was evaluated by MTT experiments and flow cytometry. RESULTS: The labeling experiments indicated that CuInS2-ZnS QDs conjugated with AFP antibody could enter HepG2 cells effectively and emit intensive yellow fluorescence by ultraviolet excitation without changing cellular morphology. Toxicity tests suggested that the cytotoxicity of CuInS2-ZnS-AFP probe was significantly lower than that of CdTe-ZnS-AFP probe (t test, F=0.8, T=-69.326, P<0.001). For CuInS2-ZnS-AFP probe, time-effect relationship was presented in intermediate concentration (>20%) groups (P<0.05) and dose-effect relationship was presented in almost all of the groups (P<0.05). CONCLUSION: CuInS2-ZnS-AFP QDs probe had better bio-compatibility and lower cytotoxicity compared with CdTe-ZnS-AFP probe, and could be used for imaging the living cells in vitro.

Facile synthesis of water-soluble Zn-doped AgIn5S8/ZnS core/shell fluorescent nanocrystals and their biological application.

Here we demonstrate a novel and facile strategy of highly luminescent water-soluble Zn-doped AgIn5S8 (ZAIS) nanocrystals and ZAIS/ZnS core/shell structures, which were based on hydrothermal reaction between the acetate salts of the corresponding metals and sulfide precursor in the presence of l-cysteine at 110 °C in a Teflon-lined autoclave. The photoluminescent (PL) emission wavelength can be conveniently tuned from 560 to 650 nm by tailoring the stoichiometric ratio of [Ag]/[Zn]. The as prepared nanocrystals were characterized systematically and exhibit long PL lifetimes more than 100 ns. The influence of experimental conditions, including concentration of l-cysteine and reaction temperature, was investigated. In addition, we performed a coating procedure with the ZnS shell outside the ZAIS core and showed excellent PL quantum yields up to 35%. The in vitro experiment exhibited quite low cytotoxicity and marvelous biocompatibility, revealing their promising prospect in bioscience. Furthermore, the obtained ZAIS/ZnS nanocompounds (NCs) were covalently conjugated to alpha-fetoprotein antibodies and targeted fluorescent imaging for hepatocellular carcinoma cells was realized.

NiFeP nanosheets for efficient and durable hydrazine-assisted electrolytic hydrogen production.

Hydrazine-assisted electrochemical water splitting is an important avenue toward low cost and sustainable hydrogen production, which can significantly reduce the voltage of electrochemical water splitting. Herein, we took a simple approach to fabricate NiFeP nanosheet arrays on nickel foam (NiFeP/NF), which exhibit superior electrocatalytic activity for the hydrogen evolution reaction (HER) and the hydrazine oxidation reaction (HzOR). Our investigations revealed that the excellent electrocatalytic activity of NiFeP/NF mainly arises from the bimetallic synergistic effect, abundant electrocatalytically active sites facilitated by the porous nanosheet morphology, high intrinsic conductivity of NiFeP/NF and strong NiFeP-NF adhesion. We assembled a hydrazine-boosted electrochemical water splitting cell using NiFeP/NF as a bifunctional catalyst for both electrodes, and the overall hydrazine splitting (OHzS) exhibits a considerably low overpotential (100 mV at 10 mA cm-2), and is stable for 40 h continuous electrolysis in a 1 M KOH + 0.5 M N2H4 electrolyte. When it is applied to hydrogen production by seawater electrolysis, its catalytic activity shows strong tolerance. This work provides a promising approach for low cost, high-efficiency and stable hydrogen production based on hydrazine-assisted electrolytic seawater splitting for future applications.

Longitudinal comparison of health locus of control and subjective well-being in older women with breast cancer.

OBJECTIVE: To investigate the dynamic changes in the health locus of control (HLC) and subjective well-being (SWB) of older women with breast cancer, to explore the relationship between the HLC and SWB of these patients at different time points. DESIGN: The research method adopted was a longitudinal study. Convenience sampling was used to select older women with breast cancer and their HLC and SWB at different times were evaluated. The ages of the patients were ≥60 years. The survey was conducted starting from the day of admission and at 1 month, 3 months, and 6 months after surgery. MAIN OUTCOME MEASURES: HLC and SWB. RESULTS: The HLC and SWB of older women with breast cancer changed dynamically (P < 0.05), and the patients were in poor condition at T1. At each time point, the internal HLC (IHLC) for these patients was positively correlated with the total SWB score. Additionally, powerful others HLC (PHLC), chance HLC (CHLC), and SWB were negatively correlated (P < 0.05). CONCLUSION: The HLC and SWB of older women with breast cancer change dynamically. Medical staff should pay more attention to the HLC and SWB of patients 1 month after surgery. SWB can be improved by improving the patients' IHLC and reducing their PHLC and CHLC.

Solar-Light-Responsive Zinc-Air Battery with Self-Regulated Charge-Discharge Performance based on Photothermal Effect.

It is extremely challenging to significantly increase the voltaic efficiency, power density, and cycle stability of a Zn-air battery by just adjusting the catalytic performance of the cathode with nanometers/atomistic engineering because of the restriction of thermodynamic equilibrium potential. Herein, inspired by solar batteries, the S-atom-bridged FeNi particles and N-doped hollow carbon nanosphere composite configuration (FeNi-S,N-HCS) is presented as a prototype of muti-functional air electrode material (intrinsic electrocatalytic function and additional photothermal function) for designing photoresponsive all-solid-state Zn-air batteries (PR-ZABs) based on the photothermal effect. The local temperature of the FeNi-S,N-HCS electrode can well respond to the stimuli of sunlight irradiation because of their superior photothermal effect. As expected, under illumination, the power density of the as-fabricated PR-ZABs based on the FeNi-S,N-HCS electrode can be improved from 77 mW cm-2 to 126 mW cm-2. Simultaneously, charge voltage can be dramatically reduced, and cycle lifetime is also prolonged under illumination, because of the expedited electrocatalytic kinetics, the increased electrical conductivity, and the accelerated desorption rate of O2 bubbles from the electrode. By exerting the intrinsic electrocatalytic and photothermal efficiency of the electrode materials, this research paves new ways to improve battery performance from kinetic and thermodynamic perspectives.

Correlation between self-awareness, communication ability and caring ability of undergraduate nursing students/A cross-sectional study.

BACKGROUND: Caring ability is the core ability of nursing. Here we explored the factors affecting the caring ability of undergraduate nursing students. Self-awareness and communication ability are potential variables to improve caring ability. OBJECTIVE: To explore the relationship between self-awareness, communication ability and caring ability of domestic undergraduate nursing students and to explore whether communication ability has an intermediary effect between self-awareness and caring ability. DESIGN: A descriptive cross-sectional study using an online questionnaire. SETTING AND PARTICIPANTS: Undergraduate nursing students (n = 1411) who attended Dalian Medical University, Liaoning, China. Participants were recruited through convenience and snowball sampling methods using online platforms. METHODS: The questionnaire used the Self-Consciousness Scale, Clinical Communication Competence Evaluation Scale and Caring Ability Inventory. The data were processed and analyzed by SPSS 24.0 and Amos 23.0. Descriptive statistics, Pearson correlation analysis and structural equation modeling were performed for data analysis. RESULTS: There was a significant positive correlation between undergraduate nursing students' caring ability and their self-awareness and communication ability (p < 0.001). Communication ability played a partial mediating role between self-awareness and caring ability (p < 0.001), and the mediating effect was 34.2 %. CONCLUSIONS: The self-awareness of undergraduate nursing students directly affects their caring ability and can also indirectly affect their caring ability through their communication ability. Educators can guide and cultivate student self-awareness and communication ability and promote self-regulation, so as to improve students' caring ability and improve clinical development.

Effects of multidisciplinary exercise management on patients after percutaneous coronary intervention: A randomized controlled study.

Objectives: To explore the effectiveness of the mobile app-based multidisciplinary exercise management on patients who receive percutaneous coronary intervention (PCI). Methods: From January to October 2020, 54 patients after PCI were randomly assigned to the intervention group (n = 27) and the control group (n = 27). The intervention group received the mobile app-based multidisciplinary exercise management, whereas the control group received routine care. The patients after PCI began to take intervention one month after the operation, and the intervention lasted for two months. Before and after the intervention, 6-Minute Walking Distance was used to evaluate the patient's exercise tolerance, and the patient's exercise compliance was evaluated according to the patient's exercise status recorded by the mobile app. The cognitive questionnaire on knowledge about PCI treatment for Coronary Heart Disease, the Self-efficacy for Chronic Disease Scale and the Perceived Social Support Scale were used to evaluate patients' disease-related cognition, self-efficacy and perception of social support. This study was registered on Clinical Trials.gov with registration number ChiCTR2000028930. Results: Totally 51 patients after PCI who completed this study (25 patients in the intervention group and 26 patients in the control group) were included in the analysis. After 2 months of intervention, the exercise compliance of patients in the intervention group was better than that in the control group. And 6-Minute Walking Distance (469.36 ± 57.48 vs. 432.81 ± 67.09), and the scores of knowledge of PCI treatment for coronary heart disease (52.64 ± 9.82 vs. 42.42 ± 8.54), Self-efficacy for Chronic Disease Scale (42.40 ± 8.04 vs. 36.88 ± 7.73) and Perceived Social Support Scale (74.04 ± 5.73 vs. 66.69 ± 6.86) in the intervention group were higher than those in the control group with statistical significance (P < 0.05). Conclusions: The multidisciplinary exercise management based on the mobile app can effectively improve exercise tolerance, exercise compliance, disease-related cognition, self-efficacy, and perception of social support during exercise training for patients after PCI.

Long Non-coding RNA RMST Worsens Ischemic Stroke via MicroRNA-221-3p/PIK3R1/TGF-ß Signaling Pathway.

Much efforts have been made to probe the mechanism underlying ischemic stroke (IS). This study was proposed to uncover the role of long non-coding RNA rhabdomyosarcoma 2 related transcript (RMST) in IS through microRNA-221-3p (miR-221-3p)/phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1)/transforming growth factor-ß (TGF-ß) axis. Neurological behavioral function, pathological changes in brain tissue, oxidative stress, and inflammation responses in middle cerebral artery occlusion (MCAO) mice were tested. RMST, miR-221-3p, PIK3R1, and TGF-ß signaling-related protein expression in brain tissues of MCAO mice were detected. RMST and PIK3R1 were elevated, miR-221-3p was downregulated, and TGF-ß pathway was activated in mice after MCAO. Restored miR-221-3p or depleted RMST improved neurological behavioral functions, relieved pathological injury in brain tissue, and repressed oxidative stress and inflammation in mice after MCAO. Depleted PIK3R1 or restored miR-221-3p offsets the negative effects of overexpressed RMST on mice with MCAO. The present work highlights that RMST augments IS through reducing miR-221-3p-mediated regulation of PIK3R1 and activating TGF-ß pathway.