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1.
Mol Cell ; 77(3): 475-487.e11, 2020 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-31759822

RESUMO

How repetitive elements, epigenetic modifications, and architectural proteins interact ensuring proper genome expression remains poorly understood. Here, we report regulatory mechanisms unveiling a central role of Alu elements (AEs) and RNA polymerase III transcription factor C (TFIIIC) in structurally and functionally modulating the genome via chromatin looping and histone acetylation. Upon serum deprivation, a subset of AEs pre-marked by the activity-dependent neuroprotector homeobox Protein (ADNP) and located near cell-cycle genes recruits TFIIIC, which alters their chromatin accessibility by direct acetylation of histone H3 lysine-18 (H3K18). This facilitates the contacts of AEs with distant CTCF sites near promoter of other cell-cycle genes, which also become hyperacetylated at H3K18. These changes ensure basal transcription of cell-cycle genes and are critical for their re-activation upon serum re-exposure. Our study reveals how direct manipulation of the epigenetic state of AEs by a general transcription factor regulates 3D genome folding and expression.


Assuntos
Elementos Alu/fisiologia , Histonas/metabolismo , Fatores de Transcrição TFIII/metabolismo , Acetilação , Elementos Alu/genética , Linhagem Celular , Cromatina/metabolismo , Cromatina/fisiologia , Epigênese Genética/genética , Regulação da Expressão Gênica/genética , Histonas/genética , Proteínas de Homeodomínio/genética , Humanos , Proteínas do Tecido Nervoso/genética , Regiões Promotoras Genéticas/genética , Processamento de Proteína Pós-Traducional , RNA Polimerase III/metabolismo , Fatores de Transcrição TFIII/genética , Transcrição Gênica/genética
3.
Nat Commun ; 12(1): 6992, 2021 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-34848735

RESUMO

Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position.


Assuntos
RNA Polimerase III/química , DNA Polimerase Dirigida por RNA/química , Proteínas de Saccharomyces cerevisiae/química , Genes Fúngicos , RNA Polimerase II/genética , RNA Polimerase II/metabolismo , RNA Polimerase III/genética , RNA Polimerase III/metabolismo , RNA de Transferência/genética , DNA Polimerase Dirigida por RNA/genética , DNA Polimerase Dirigida por RNA/metabolismo , Retroelementos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Fator de Transcrição TFIIIB/metabolismo
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