Public perceptions of trophy hunting are pragmatic, not dogmatic.

Fierce international debates rage over whether trophy hunting is socially acceptable, especially when people from the Global North hunt well-known animals in sub-Saharan Africa. We used an online vignette experiment to investigate public perceptions of the acceptability of trophy hunting in sub-Saharan Africa among people who live in urban areas of the USA, UK and South Africa. Acceptability depended on specific attributes of different hunts as well as participants' characteristics. Zebra hunts were more acceptable than elephant hunts, hunts that would provide meat to local people were more acceptable than hunts in which meat would be left for wildlife, and hunts in which revenues would support wildlife conservation were more acceptable than hunts in which revenues would support either economic development or hunting enterprises. Acceptability was generally lower among participants from the UK and those who more strongly identified as an animal protectionist, but higher among participants with more formal education, who more strongly identified as a hunter, or who would more strongly prioritize people over wild animals. Overall, acceptability was higher when hunts would produce tangible benefits for local people, suggesting that members of three urban publics adopt more pragmatic positions than are typically evident in polarized international debates.

Practical advice on variable selection and reporting using Akaike information criterion.

The various debates around model selection paradigms are important, but in lieu of a consensus, there is a demonstrable need for a deeper appreciation of existing approaches, at least among the end-users of statistics and model selection tools. In the ecological literature, the Akaike information criterion (AIC) dominates model selection practices, and while it is a relatively straightforward concept, there exists what we perceive to be some common misunderstandings around its application. Two specific questions arise with surprising regularity among colleagues and students when interpreting and reporting AIC model tables. The first is related to the issue of 'pretending' variables, and specifically a muddled understanding of what this means. The second is related to p-values and what constitutes statistical support when using AIC. There exists a wealth of technical literature describing AIC and the relationship between p-values and AIC differences. Here, we complement this technical treatment and use simulation to develop some intuition around these important concepts. In doing so we aim to promote better statistical practices when it comes to using, interpreting and reporting models selected when using AIC.

Parsing variance by marker type: Testing biogeographic hypotheses and differential contribution of historical processes to population structure in a desert lizard.

A fundamental goal of population genetic studies is to identify historical biogeographic patterns and understand the processes that generate them. However, localized demographic events can skew population genetic inference. Assessing populations with multiple types of genetic markers, each with unique mutation rates and responses to changes in population size, can help to identify potentially confounding population-specific demographic processes. Here, we compared population structure and connectivity inferred from microsatellites and restriction site-associated DNA loci among 17 populations of an arid-specialist lizard, the desert night lizard, Xantusia vigilis, in central California to test among historical processes structuring population genetic diversity. We found that both marker types yielded generally concordant insights into population genetic structure including a major phylogenetic break maintained between two populations separated by less than 10 km, suggesting that either marker type could be used to understand generalized demographic patterns across the region for management purposes. However, we also found that the effects of demography on marker discordance could be used to elucidate population histories and distinguish among competing biogeographic hypotheses. Our results suggest that comparisons of within-population diversity across marker types provide powerful opportunities for leveraging marker discordance, particularly for understanding the creation and maintenance of contact zones among clades.

Ex situ management as insurance against extinction of mammalian megafauna in an uncertain world.

The persistence of endangered species may depend on the fate of a very small number of individual animals. In situ conservation alone may sometimes be insufficient. In these instances, the International Union for Conservation of Nature provides guidelines for ex situ conservation and the Convention on Biological Diversity (CBD) indicates how ex situ management can support the CBD's objectives by providing insurance policies for species. The circumstances that justify its use are uncertain. To evaluate the current in situ extinction risk and ex situ management of 43 critically endangered species of mammalian megafauna, we used nonmetric multidimensional scaling and geopolitical variables related to governance, economics, and national policy within their extant ranges. We then fitted generalized additive models to assess the contribution of each variable to the ordination. Fifteen (almost one-third) of the world's terrestrial mammalian megafauna are not the subject of any ex situ management. Seventy-three percent of these taxa occur in areas characterized by political uncertainty, such as border zones or areas affected by armed conflicts, mainly in Africa and the Middle East. A further 23% of taxa in ex situ programs do not meet sustainability criteria for inbreeding avoidance. Strategic conservation planning, such as the One Plan approach, may improve ex situ management for these taxa. Given the escalating trend in threats afflicting megafauna, ex situ management should be considered more rigorously, particularly in politically unstable regions, to achieve CBD Target 12 (prevent extinction of threatened species).

Manejo Ex Situ como Protección contra la Extinción de la Megafauna de Mamíferos en un Mundo Incierto Resumen La persistencia de las especies en peligro puede depender del destino de un número muy pequeño de animales individuales. La conservación in situ por sí sola a veces puede ser insuficiente. Bajo estas instancias, la Unión Internacional para la Conservación de la Naturaleza proporciona directrices para la conservación ex situ y la Convención sobre la Diversidad Biológica (CBD) indica cómo el manejo ex situ puede apoyar a lograr sus objetivos al proporcionar políticas de protección para las especies. Las circunstancias que justifican el uso del manejo ex situ son inciertas. Para evaluar el actual riesgo de extinción in situ y el manejo ex situ de 43 especies de megafauna de mamíferos en peligro crítico de extinción usamos un escalamiento multidimensional no métrico, así como variables geopolíticas relacionadas con el gobierno, la economía y las políticas nacionales dentro de la distribución actual de estas especies. Después ajustamos los modelos sumativos generalizados para evaluar la contribución de cada variable a la ordinación. Quince (casi un tercio) de los mamíferos terrestres pertenecientes a la megafauna no están sujetos a ningún tipo de manejo ex situ. El 73% de estos taxones ocurren en áreas conocidas por su incertidumbre política, como zonas fronterizas o áreas afectadas por conflictos armados, principalmente en África y en el Oriente Medio. Además, un 23% de los taxones que sí se encuentran en programas de conservación ex situ no cumplen con los criterios de sustentabilidad para evitar la endogamia. La planeación de la conservación estratégica, como la estrategia de One Plan, podría mejorar el manejo ex situ para estos taxones. Dada la tendencia creciente en las amenazas que afectan a la megafauna, el manejo ex situ debería considerarse de manera más rigurosa, particularmente en las regiones con inestabilidad política, para alcanzar el Objetivo 12 de la CBD (evitar la extinción de especies amenazadas).

Daily energy expenditure in the face of predation: hedgehog energetics in rural landscapes.

Failure to balance daily energy expenditure (DEE) with energy intake can have an impact on survival and reproduction, and therefore on the persistence of populations. Here we study the DEE of the European hedgehog (Erinaceus europaeus), which is declining in the UK. We hypothesise that there is a gradient of suitable habitat for hedgehogs in rural areas, which is a result of fewer food resources, a higher risk from predation by badgers (Meles meles) and colder ambient temperatures, as distance to the nearest building increases. We used the doubly labelled water method to obtain 44 measurements of DEE from hedgehogs on four predominately arable sites, to determine the energetic costs associated with proximity to buildings, on sites with and without badgers. The mean±s.e.m. DEE was 508.9±34.8 kJ day-1 DEE increased the further a hedgehog was from buildings during the study, possibly as they ranged larger distances on arable land, supporting the hypothesis that hedgehogs select villages owing to the lower energy demands in comparison to arable farmland. Hedgehogs had an approximately 30% lower DEE on sites with badgers. We speculate that on badger-occupied sites, hedgehogs may restrict movement and foraging in response to a threat from predation and thus have reduced DEE. Therefore, hedgehogs may also seek refuge in villages where the perceived threat of predation is lower and foraging is unrestricted. In a broader context, we demonstrate that individual differences in DEE can aid in understanding habitat selection in a patchily distributed species.

Pharmacodynamics, population dynamics, and the evolution of persistence in Staphylococcus aureus.

When growing populations of bacteria are confronted with bactericidal antibiotics, the vast majority of cells are killed, but subpopulations of genetically susceptible but phenotypically resistant bacteria survive. In accord with the prevailing view, these "persisters" are non- or slowly dividing cells randomly generated from the dominant population. Antibiotics enrich populations for pre-existing persisters but play no role in their generation. The results of recent studies with Escherichia coli suggest that at least one antibiotic, ciprofloxacin, can contribute to the generation of persisters. To more generally elucidate the role of antibiotics in the generation of and selection for persisters and the nature of persistence in general, we use mathematical models and experiments with Staphylococcus aureus (Newman) and the antibiotics ciprofloxacin, gentamicin, vancomycin, and oxacillin. Our results indicate that the level of persistence varies among these drugs and their concentrations, and there is considerable variation in this level among independent cultures and mixtures of independent cultures. A model that assumes that the rate of production of persisters is low and persisters grow slowly in the presence of antibiotics can account for these observations. As predicted by this model, pre-treatment with sub-MIC concentrations of antibiotics substantially increases the level of persistence to drugs other than those with which the population is pre-treated. Collectively, the results of this jointly theoretical and experimental study along with other observations support the hypothesis that persistence is the product of many different kinds of errors in cell replication that result in transient periods of non-replication and/or slowed metabolism by individual cells in growing populations. This Persistence as Stuff Happens (PaSH) hypothesis can account for the ubiquity of this phenomenon. Like mutation, persistence is inevitable rather than an evolved character. What evolved and have been identified are genes and processes that affect the frequency of persisters.

The pharmaco -, population and evolutionary dynamics of multi-drug therapy: experiments with S. aureus and E. coli and computer simulations.

There are both pharmacodynamic and evolutionary reasons to use multiple rather than single antibiotics to treat bacterial infections; in combination antibiotics can be more effective in killing target bacteria as well as in preventing the emergence of resistance. Nevertheless, with few exceptions like tuberculosis, combination therapy is rarely used for bacterial infections. One reason for this is a relative dearth of the pharmaco-, population- and evolutionary dynamic information needed for the rational design of multi-drug treatment protocols. Here, we use in vitro pharmacodynamic experiments, mathematical models and computer simulations to explore the relative efficacies of different two-drug regimens in clearing bacterial infections and the conditions under which multi-drug therapy will prevent the ascent of resistance. We estimate the parameters and explore the fit of Hill functions to compare the pharmacodynamics of antibiotics of four different classes individually and in pairs during cidal experiments with pathogenic strains of Staphylococcus aureus and Escherichia coli. We also consider the relative efficacy of these antibiotics and antibiotic pairs in reducing the level of phenotypically resistant but genetically susceptible, persister, subpopulations. Our results provide compelling support for the proposition that the nature and form of the interactions between drugs of different classes, synergy, antagonism, suppression and additivity, has to be determined empirically and cannot be inferred from what is known about the pharmacodynamics or mode of action of these drugs individually. Monte Carlo simulations of within-host treatment incorporating these pharmacodynamic results and clinically relevant refuge subpopulations of bacteria indicate that: (i) the form of drug-drug interactions can profoundly affect the rate at which infections are cleared, (ii) two-drug therapy can prevent treatment failure even when bacteria resistant to single drugs are present at the onset of therapy, and (iii) this evolutionary virtue of two-drug therapy is manifest even when the antibiotics suppress each other's activity.

Correlates of bushmeat in markets and depletion of wildlife.

We used data on number of carcasses of wildlife species sold in 79 bushmeat markets in a region of Nigeria and Cameroon to assess whether species composition of a market could be explained by anthropogenic pressures and environmental variables around each market. More than 45 mammal species from 9 orders were traded across all markets; mostly ungulates and rodents. For each market, we determined median body mass, species diversity (game diversity), and taxa that were principal contributors to the total number of carcasses for sale (game dominance). Human population density in surrounding areas was significantly and negatively related to the percentage ungulates and primates sold in markets and significantly and positively related to the proportion of rodents. The proportion of carnivores sold was higher in markets with high human population densities. Proportion of small-bodied mammals (<1 kg) sold in markets increased as human population density increased, but proportion of large-bodied mammals (>10 kg) decreased as human population density increased. We calculated an index of game depletion (GDI) for each market from the sum of the total number of carcasses traded per annum and species, weighted by the intrinsic rate of natural increase (rmax ) of each species, divided by individuals traded in a market. The GDI of a market increased as the proportion of fast-reproducing species (highest rmax ) increased and as the representation of species with lowest rmax (slow-reproducing) decreased. The best explanatory factor for a market's GDI was anthropogenic pressure-road density, human settlements with >3000 inhabitants, and nonforest vegetation. High and low GDI were significantly differentiated by human density and human settlements with >3000 inhabitants. Our results provided empirical evidence that human activity is correlated with more depleted bushmeat faunas and can be used as a proxy to determine areas in need of conservation action.

Output control of da Vinci surgical system's surgical graspers.

INTRODUCTION: The number of robot-assisted surgeries performed with the da Vinci surgical system has increased significantly over the past decade. The articulating movements of the robotic surgical grasper are controlled by grip controls at the master console. The user interface has been implicated as one contributing factor in surgical grasping errors. The goal of our study was to characterize and evaluate the user interface of the da Vinci surgical system in controlling surgical graspers. MATERIALS AND METHODS: An angular manipulator with force sensors was used to increment the grip control angle as grasper output angles were measured. Input force at the grip control was simultaneously measured throughout the range of motion. Pressure film was used to assess the maximum grasping force achievable with the endoscopic grasping tool. RESULTS: The da Vinci robot's grip control angular input has a nonproportional relationship with the grasper instrument output. The grip control mechanism presents an intrinsic resistant force to the surgeon's fingertips and provides no haptic feedback. The da Vinci Maryland graspers are capable of applying up to 5.1 MPa of local pressure. CONCLUSIONS: The angular and force input at the grip control of the da Vinci robot's surgical graspers is nonproportional to the grasper instrument's output. Understanding the true relationship of the grip control input to grasper instrument output may help surgeons understand how to better control the surgical graspers and promote fewer grasping errors.

Mapping hotspots of threatened species traded in bushmeat markets in the Cross-Sanaga rivers region.

Bushmeat markets exist in many countries in West and Central Africa, and data on species sold can be used to detect patterns of wildlife trade in a region. We surveyed 89 markets within the Cross-Sanaga rivers region, West Africa. In each market, we counted the number of carcasses of each taxon sold. During a 6-month period (7594 market days), 44 mammal species were traded. Thirteen species were on the International Union for Conservation of Nature (IUCN) Red List or protected under national legislation, and at least 1 threatened species was traded in 88 of the 89 markets. We used these data to identify market groups that traded similar species assemblages. Using cluster analyses, we detected 8 market groups that were also geographically distinct. Market groups differed in the diversity of species, evenness of species, and dominant, prevalent, and characteristic species traded. We mapped the distribution of number of threatened species traded across the study region. Most threatened species were sold in markets nearest 2 national parks, Korup National Park in Cameroon and Cross River in Nigeria. To assess whether the threatened-species trade hotspots coincided with the known ranges of these species, we mapped the overlap of all threatened species traded. Markets selling more threatened species overlapped with those regions that had higher numbers of these. Our study can provide wildlife managers in the region with better tools to discern zones within which to focus policing efforts and reduce threats to species that are threatened by the bushmeat trade.

Resection of isolated local and metastatic recurrence in periampullary adenocarcinoma.

BACKGROUND: The majority of patients with periampullary cancer develop local or metastatic recurrence despite successful negative margin resection. Unfortunately, there are no established therapeutic strategies for managing these patients. The literature on the surgical resection of recurrent disease is limited. METHODS: This is a retrospective study evaluating patients who underwent reoperative resection of recurrent periampullary cancer at a single institution between 1990 and 2011. Perioperative outcomes were compared with those of the original primary resections for patients with local recurrence. Kaplan-Meier curves were used to evaluate survival. RESULTS: Twenty-two patients underwent reoperative resection following the successful primary resection of periampullary cancers. Median survival from the time of reoperation was 28.1 months. A greater survival benefit was seen in patients undergoing reoperative resection with >15 months between the primary resection and recurrence (40.6 months versus 8.2 months; P < 0.05). Complication rates were lower after reoperative resection compared with the primary resection (20% versus 70%). Perioperative characteristics including operative time, estimated blood loss and hospital stay were similar in both the primary and reoperation procedures. CONCLUSIONS: Surgical resection of periampullary cancer recurrence is feasible, safe and may offer survival benefits in comparison with alternative treatment modalities. Reoperative resection should be considered, especially in patients in whom the time to recurrence is lengthy.

Matrix-based key to the click beetle genera of Canada and USA with a summary of habitat use (Coleoptera, Elateridae).

The Elateridae, or click beetles are abundant and diverse in most terrestrial ecosystems in North America, acting as plant pests and filling many other ecological roles. The 112 genera of Elateridae Leach, 1815, or click beetles, known from Canada and USA are included in a first comprehensive digital interactive key to adults. A link to an online peer-reviewed LUCID key to elaterid genera and downloadable LUCID files are provided. Diagnostic morphological summaries using information from the 61 characters and 158 character states of the matrix key are presented for all genera. A table summarizes current understanding of habitat use by all elaterid genera in Canada and USA from literature, collections, citizen science, and our own observations. Diversity of elaterid genera was high throughout warm and cool temperate regions, especially in mountainous areas and mesic woodlands. Larvae of most genera were associated with soil, litter and decaying wood.

A new species of Platygaster Latreille (Hymenoptera: Platygastridae) parasitizing Chilophaga virgati Gagné (Diptera: Cecidomyiidae).

Platygaster chilophagae, new species, is described from specimens reared from larvae of Chilophaga virgati Gagné collected and reared in eastern South Dakota. The host larva feeds on the basal meristematic tissues of the inflorescence of Panicum virgatum L. This new species seems to lack immediate affinities with any described Platygaster species in its combination of characteristics, and is compared to and discriminated from six other species. Polyembryony is suggested by the presence of cocoon clusters containing 4-14 pupae from each host larva.

MpeR regulates the mtr efflux locus in Neisseria gonorrhoeae and modulates antimicrobial resistance by an iron-responsive mechanism.

Previous studies have shown that the MpeR transcriptional regulator produced by Neisseria gonorrhoeae represses the expression of mtrF, which encodes a putative inner membrane protein (MtrF). MtrF works as an accessory protein with the Mtr efflux pump, helping gonococci to resist high levels of diverse hydrophobic antimicrobials. Regulation of mpeR has been reported to occur by an iron-dependent mechanism involving Fur (ferric uptake regulator). Collectively, these observations suggest the presence of an interconnected regulatory system in gonococci that modulates the expression of efflux pump protein-encoding genes in an iron-responsive manner. Herein, we describe this connection and report that levels of gonococcal resistance to a substrate of the mtrCDE-encoded efflux pump can be modulated by MpeR and the availability of free iron. Using microarray analysis, we found that the mtrR gene, which encodes a direct repressor (MtrR) of mtrCDE, is an MpeR-repressed determinant in the late logarithmic phase of growth when free iron levels would be reduced due to bacterial consumption. This repression was enhanced under conditions of iron limitation and resulted in increased expression of the mtrCDE efflux pump operon. Furthermore, as judged by DNA-binding analysis, MpeR-mediated repression of mtrR was direct. Collectively, our results indicate that both genetic and physiologic parameters (e.g., iron availability) can influence the expression of the mtr efflux system and modulate levels of gonococcal susceptibility to efflux pump substrates.

Current trends suggest most Asian countries are unlikely to meet future biodiversity targets on protected areas.

Aichi Target 11 committed governments to protect ≥17% of their terrestrial environments by 2020, yet it was rarely achieved, raising questions about the post-2020 Global Biodiversity Framework goal to protect 30% by 2030. Asia is a challenging continent for such targets, combining high biodiversity with dense human populations. Here, we evaluated achievements in Asia against Aichi Target 11. We found that Asia was the most underperforming continent globally, with just 13.2% of terrestrial protected area (PA) coverage, averaging 14.1 ± SE 1.8% per country in 2020. 73.1% of terrestrial ecoregions had <17% representation and only 7% of PAs even had an assessment of management effectiveness. We found that a higher agricultural land in 2015 was associated with lower PA coverage today. Asian countries also showed a remarkably slow average annual pace of 0.4 ± SE 0.1% increase of PA extent. These combined lines of evidence suggest that the ambitious 2030 targets are unlikely to be achieved in Asia unless the PA coverage to increase 2.4-5.9 times faster. We provided three recommendations to support Asian countries to meet their post-2020 biodiversity targets: complete reporting and the wider adoption "other effective area-based conservation measures"; restoring disturbed landscapes; and bolstering transboundary PAs.

Off-target gene regulation mediated by transcriptional repressors of antimicrobial efflux pump genes in Neisseria gonorrhoeae.

DNA-binding proteins that control expression of drug efflux pump genes have been termed "local regulators" as their encoding gene is often located adjacent to the gene(s) that they regulate. However, results from recent studies indicate that they can control genes outside efflux pump-encoding loci, which we term as being "off target." For example, the MtrR repressor was initially recognized for its ability to repress transcription of the mtrCDE-encoded efflux pump operon in the strict human pathogen Neisseria gonorrhoeae, but recent results from genetic and microarray studies have shown that it can control expression of nearly 70 genes scattered throughout the chromosome. One of the off-target MtrR-repressed genes is glnA, which encodes glutamine synthetase. Herein, we confirm the capacity of MtrR to repress glnA expression and provide evidence that such repression is due to its ability to negatively influence the binding of a second DNA-binding protein (FarR), which activates glnA. FarR was previously recognized as a transcriptional repressor of the farAB-encoded efflux pump operon. Thus, two DNA-binding proteins previously characterized as repressors of genes encoding efflux pumps that contribute to gonococcal resistance to antimicrobials can act in an opposing manner to modulate expression of a gene involved in basic metabolism.

Association of body mass with price of bushmeat in Nigeria and Cameroon.

Spatially extensive patterns of bushmeat extraction (and the processes underlying these patterns) have not been explored. We used data from a large sample (n= 87) of bushmeat trading points in urban and rural localities in Nigeria and Cameroon to explore extraction patterns at a regional level. In 7,594 sample days, we observed 61,267 transactions involving whole carcasses. Rural and urban trading points differed in species for sale and in meat condition (fresh or smoked). Carcass price was principally associated with body mass, with little evidence that taxonomic group (primate, rodent, ungulate, or mammalian carnivore) affected price. Moreover, meat condition was not consistently associated with price. However, some individual species were more expensive throughout the region than would be expected for their size. Prices were weakly positively correlated with human settlement size and were highest in urban areas. Supply did not increase proportionally as human settlement size increased, such that per capita supply was significantly lower in urban centers than in rural areas. Policy options, including banning hunting of more vulnerable species (those that have low reproductive rates), may help to conserve some species consumed as bushmeat because carcass prices indicate that faster breeding, and therefore the more sustainable species, may be substituted and readily accepted by consumers.

Resumen: Los patrones espacialmente extensivos de extracción de carne silvestre (y los procesos relacionados con esos patrones) no han sido explorados. Utilizamos datos de una muestra grande (n =87) de puntos de venta de carne silvestre en localidades urbanas y rurales en Nigeria y Camerún para explorar los patrones de extracción a nivel regional. En 7,594 días de muestreo observamos 61,267 transacciones involucrando cuerpos completos. Los puntos de venta rurales y urbanos difirieron en especies en venta y condición de la carne (fresca o ahumada). El precio del cuerpo se asoció principalmente con la masa corporal, con poca evidencia de que el grupo taxonómico (primate, roedor, ungulado o mamífero carnívoro) afectara el precio. Mas aun, la condición de la carne no se asoció consistentemente con el precio. Sin embargo, algunas especies individuales en la región fueron más caras de lo esperado por su tamaño. Los precios se correlacionaron positiva y débilmente con el tamaño del asentamiento humano y fueron más altos en áreas urbanas. La oferta no incrementó proporcionalmente a medida que incrementó el tamaño del asentamiento, tanto que la oferta per cápita fue significativamente menor en centros urbanos que en áreas urbanas. Opciones de políticas, incluyendo la prohibición de la caza de especies más vulnerables (aquellas que tienen bajas tasas reproductivas), pueden ayudar a conservar algunas especies consumidas como carne silvestre porque los precios indican que especies de reproducción más rápida, y por lo tanto más sustentables, pueden ser sustituidas y aceptadas de buena gana por los consumidores.

Development and testing of a vaginal pressure sensor to measure intra-abdominal pressure in women.

AIMS: Elevated intra-abdominal pressure (IAP) may be a potentially modifiable risk factor for pelvic floor disorders. However, limited evidence exists due to the lack of instruments suitable to measure abdominal pressures in real world settings. The aim of this study was to develop and test a vaginal sensor prototype to measure intra-abdominal pressure in women. METHODS: We developed a non-directional vaginal sensor by housing pressure-sensing circuit boards in 1.2 x 3 cm radially symmetric silicon capsules. We characterized the response in a standardized pressure chamber. Eight women wore a sensor intra-vaginally while undergoing filling cystometry. We compared peak pressures during coughing, valsalva, squatting, and jumping to those obtained using a #10 French rectal balloon urodynamics catheter. We calculated Pearson's correlation coefficients between rectal and vaginal sensors for each event. RESULTS: The vaginal sensors exhibited linear responses during initial bench testing. Each transducer correlated well with the rectal balloon catheter during coughing, valsalva, and squatting (r = 0.97, 0.94, and 0.97, respectively). However, the rectal balloon catheter recorded higher peak and lower, often negative, trough pressures during jumping. The vaginal sensor showed no such artifact. CONCLUSIONS: This vaginal pressure sensor can be used as a surrogate for measuring intra-abdominal pressure in women without advanced prolapse. By measuring pressure at the physiological source, the vaginal sensor is less prone to extraneous noise and error than current transducers. Using this prototype, we will next develop a remote wireless version to capture a range of abdominal pressures experienced outside of the laboratory setting.