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1.
Biosci Microbiota Food Health ; 42(1): 34-48, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36660591

RESUMO

The genus Bifidobacterium comprises beneficial intestinal bacteria that play a crucial role in the regulation of human health. Traditional prebiotics are known to increase intestinal bifidobacteria by supplying a carbon source necessary for their growth. However, intestinal bifidobacteria need not only a carbon source but also a nitrogen source for growth. Moreover, the growth of bifidobacteria is known to be inhibited in a culture medium that does not contain glutamic acid. Based on these reports, we hypothesized that the combined intake of traditional prebiotics and glutamic acid would be beneficial for growth of bifidobacteria in the gut. In this study, we investigated the effects of the combination of galactooligosaccharide (GOS; traditional prebiotic material) and poly-γ-glutamic acid (γ-PGA; source of glutamic acid) and only GOS on the intestinal microbiota and health conditions (including intestinal regulation, mood status, gastrointestinal condition, skin condition, and sleep quality) in a randomized, double-blind, parallel-group comparison trial in healthy subjects. The combined intake of GOS and γ-PGA significantly increased the prevalence of B. longum compared to the intake of GOS alone. A minimum effective dose of 2.0 g GOS and 0.3 g γ-PGA improved defecation and mood status. We revealed the combined effects of GOS and γ-PGA on intestinal microbiota as well as physical condition and concluded that the delivery of glutamic acid to the large intestine with traditional prebiotics is useful as an advanced prebiotic.

2.
Cell Metab ; 35(2): 361-375.e9, 2023 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-36652945

RESUMO

Although recent studies have highlighted the impact of gut microbes on the progression of obesity and its comorbidities, it is not fully understood how these microbes promote these disorders, especially in terms of the role of microbial metabolites. Here, we report that Fusimonas intestini, a commensal species of the family Lachnospiraceae, is highly colonized in both humans and mice with obesity and hyperglycemia, produces long-chain fatty acids such as elaidate, and consequently facilitates diet-induced obesity. High fat intake altered the expression of microbial genes involved in lipid production, such as the fatty acid metabolism regulator fadR. Monocolonization with a FadR-overexpressing Escherichia coli exacerbated the metabolic phenotypes, suggesting that the change in bacterial lipid metabolism is causally involved in disease progression. Mechanistically, the microbe-derived fatty acids impaired intestinal epithelial integrity to promote metabolic endotoxemia. Our study thus provides a mechanistic linkage between gut commensals and obesity through the overproduction of microbe-derived lipids.


Assuntos
Ácidos Graxos , Microbioma Gastrointestinal , Humanos , Animais , Camundongos , Dieta Hiperlipídica , Obesidade/metabolismo , Bactérias/genética , Camundongos Endogâmicos C57BL
3.
Microbiol Spectr ; 10(2): e0191521, 2022 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-35234490

RESUMO

Standardization and quality assurance of microbiome community analysis by high-throughput DNA sequencing require widely accessible and well-characterized reference materials. Here, we report on newly developed DNA and whole-cell mock communities to serve as control reagents for human gut microbiota measurements by shotgun metagenomics and 16S rRNA gene amplicon sequencing. The mock communities were formulated as near-even blends of up to 20 bacterial species prevalent in the human gut, span a wide range of genomic guanine-cytosine (GC) contents, and include multiple strains with Gram-positive type cell walls. Through a collaborative study, we carefully characterized the mock communities by shotgun metagenomics, using previously developed standardized protocols for DNA extraction and sequencing library construction. Further, we validated fitness of the mock communities for revealing technically meaningful differences among protocols for DNA extraction and metagenome/16S rRNA gene amplicon library construction. Finally, we used the mock communities to reveal varying performance of metagenome-based taxonomic profilers and the impact of trimming and filtering of sequencing reads on observed species profiles. The latter showed that aggressive preprocessing of reads may result in substantial GC-dependent bias and should thus be carefully evaluated to minimize unintended effects on species abundances. Taken together, the mock communities are expected to support a myriad of applications that rely on well-characterized control reagents, ranging from evaluation and optimization of methods to assessment of reproducibility in interlaboratory studies and routine quality control. IMPORTANCE Application of high-throughput DNA sequencing has greatly accelerated human microbiome research and its translation into new therapeutic and diagnostic capabilities. Microbiome community analyses results can, however, vary considerably across studies or laboratories, and establishment of measurement standards to improve accuracy and reproducibility has become a priority. The here-developed mock communities, which are available from the NITE Biological Resource Center (NBRC) at the National Institute of Technology and Evaluation (NITE, Japan), provide well-characterized control reagents that allow users to judge the accuracy of their measurement results. Widespread and consistent adoption of the mock communities will improve reproducibility and comparability of microbiome community analyses, thereby supporting and accelerating human microbiome research and development.


Assuntos
Microbiota , DNA , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Indicadores e Reagentes , Metagenômica/métodos , Microbiota/genética , RNA Ribossômico 16S/genética , Reprodutibilidade dos Testes , Análise de Sequência de DNA/métodos
4.
Microbiome ; 9(1): 95, 2021 04 29.
Artigo em Inglês | MEDLINE | ID: mdl-33910647

RESUMO

BACKGROUND: Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metagenomics-based microbiome profiling of human fecal samples. RESULTS: In the first phase, we performed a head-to-head comparison of a wide range of protocols for DNA extraction and sequencing library construction using defined mock communities, to identify performant protocols and pinpoint sources of inaccuracy in quantification. In the second phase, we validated performant protocols with respect to their variability of measurement results within a single laboratory (that is, intermediate precision) as well as interlaboratory transferability and reproducibility through an industry-based collaborative study. We further ascertained the performance of our recommended protocols in the context of a community-wide interlaboratory study (that is, the MOSAIC Standards Challenge). Finally, we defined performance metrics to provide best practice guidance for improving measurement consistency across methods and laboratories. CONCLUSIONS: The validated protocols and methodological guidance for DNA extraction and library construction provided in this study expand current best practices for metagenomic analyses of human fecal microbiota. Uptake of our protocols and guidelines will improve the accuracy and comparability of metagenomics-based studies of the human microbiome, thereby facilitating development and commercialization of human microbiome-based products. Video Abstract.


Assuntos
Metagenômica , Microbiota , DNA , Humanos , Microbiota/genética , Padrões de Referência , Reprodutibilidade dos Testes , Análise de Sequência de DNA
5.
Microbiol Resour Announc ; 9(17)2020 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-32327522

RESUMO

We report the complete genome sequence of Flavonifractor plautii JCM 32125T (=VPI 0310T). The genome consists of a single circular chromosome of 3,985,392 bp (G+C content, 60.9%) and was predicted to contain 3 complete sets of rRNA genes, 63 tRNA genes, and 3,764 protein-coding sequences.

6.
Microbiol Resour Announc ; 9(17)2020 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-32327523

RESUMO

We report a complete genome sequence of Blautia producta JCM 1471T The genome consists of a single circular chromosome of 6,197,116 bp with a G+C content of 45.7%. The genome was annotated as containing 5 complete sets of rRNA genes, 70 tRNA genes, and 5,516 protein-coding sequences.

7.
Microbiol Resour Announc ; 9(16)2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32299873

RESUMO

We report a complete genome sequence of Collinsella aerofaciens JCM 10188T (=VPI 1003T). The genome consists of a circular chromosome (2,428,218 bp with 60.6% G+C content) and two extrachromosomal elements. The genome was predicted to contain 5 sets of rRNA genes, 58 tRNA genes, and 2,079 protein-encoding sequences.

8.
Microbiol Resour Announc ; 9(16)2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32299874

RESUMO

We announce the complete genome sequence of Megamonas funiformis JCM 14723T (YIT 11815T). The genome consists of a circular chromosome (2,522,577 bp, 31.5% G+C content) and a plasmid of 46,189 bp (29.4% G+C content). The genome was predicted to contain 6 rRNA operons, 53 tRNA genes, and 2,440 protein-coding sequences.

9.
Sci Rep ; 7(1): 18087, 2017 12 22.
Artigo em Inglês | MEDLINE | ID: mdl-29273795

RESUMO

Our previous study shows that an anaerobic intestinal bacterium strain AJ110941P contributes to type 2 diabetes development in mice. Here we phylogenetically and physiologically characterized this unique mouse gut bacterium. The 16S rRNA gene analysis revealed that the strain belongs to the family Lachnospiraceae but shows low sequence similarities ( < 92.5%) to valid species, and rather formed a distinct cluster with uncultured mouse gut bacteria clones. In metagenomic database survey, the 16S sequence of AJ110941P also matched with mouse gut-derived datasets (56% of total datasets) with > 99% similarity, suggesting that AJ110941P-related bacteria mainly reside in mouse digestive tracts. Strain AJ110941P shared common physiological traits (e.g., Gram-positive, anaerobic, mesophilic, and fermentative growth with carbohydrates) with relative species of the Lachnospiraceae. Notably, the biofilm-forming capacity was found in both AJ110941P and relative species. However, AJ110941P possessed far more strong ability to produce biofilm than relative species and formed unique structure of extracellular polymeric substances. Furthermore, AJ110941P cells are markedly long fusiform-shaped rods (9.0-62.5 µm) with multiple flagella that have never been observed in any other Lachnospiraceae members. Based on the phenotypic and phylogenetic features, we propose a new genus and species, Fusimonas intestini gen. nov., sp. nov. for strain AJ110941P (FERM BP-11443).


Assuntos
Clostridiales/genética , Diabetes Mellitus Tipo 2/microbiologia , RNA Ribossômico 16S/genética , Animais , Técnicas de Tipagem Bacteriana , Camundongos , Análise de Sequência de DNA
10.
Microbes Environ ; 29(4): 427-30, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25283478

RESUMO

The aim of the present study was to identify bacteria that may contribute to the onset of metabolic dysfunctions. We isolated and identified a candidate bacterium belonging to Lachnospiraceae (strain AJ110941) in the feces of hyperglycemic obese mice. The colonization of germ-free ob/ob mice by AJ110941 induced significant increases in fasting blood glucose levels as well as liver and mesenteric adipose tissue weights, and decreases in plasma insulin levels and HOMA-ß values. These results indicated that the specific gut commensal bacterium AJ110941 influenced the development of obesity and diabetes in ob/ob mice with genetic susceptibility for obesity.


Assuntos
Clostridiales/crescimento & desenvolvimento , Diabetes Mellitus/microbiologia , Trato Gastrointestinal/microbiologia , Infecções por Bactérias Gram-Positivas/complicações , Animais , Glicemia/análise , DNA Bacteriano/química , DNA Bacteriano/genética , Fezes/microbiologia , Insulina/sangue , Fígado/patologia , Mesentério/patologia , Camundongos Obesos , Dados de Sequência Molecular , Análise de Sequência de DNA
11.
Environ Toxicol ; 19(1): 20-5, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-14758590

RESUMO

The correlation between the content of three microcystins (types LR, RR and YR) and the cell cycle of an axenic strain of Microcystis viridis, NIES-102, was investigated under conditions of high (16 mg L(-1)) and low (1.0 mg L(-1)) nitrate (NO(3)-N) concentrations. Each phase of the cell cycle was identified using a flow cytometer equipped with a 488-nm argon laser using SYTOX Green dye, which binds specifically to nucleic acids and can be exited by the wavelength (Ex/Em: 504/523 nm on DNA). Microcystin concentration showed a positive linear correlation with DNA concentration. The microcystin content of the cells changed remarkably as the cell cycle process proceeded, with maximum content in the G(2)/M phase and minimum content in the G(0)/G(1) phase. Under a condition of high NO(3)-N concentration, the ratio of the total content in the G(0)/G(1) phase to that in the G(2)/M phase was about 6:1. In contrast, for the two batch cultures the total content was 1.3-fold greater in the G(2)/M phase. The compositions of the three microcystins also changed along with the cell cycle process, although there was little difference in composition that was related to NO(3)-N concentration. Therefore, there were distinctive compositions specific to each phase of the cycle, and the cell cycle of the M. viridis strain was more strongly responsible for both the quantity and the types of microcystin production than was the effect of NO(3)-N concentration.


Assuntos
Ciclo Celular/fisiologia , Microcystis/fisiologia , Peptídeos Cíclicos/biossíntese , DNA Bacteriano/análise , Toxinas Marinhas , Microcistinas
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