RESUMO
The gut microbiota and neurological development of neonatal mice are susceptible to environmental factors that may lead to altered behavior into adulthood. However, the role that changed gut microbiota and neurodevelopment early in life play in this needs to be clarified. In this study, by modeling early-life environmental changes by cross-fostering BALB/c mice, we revealed the effects of the environment during the critical period of postnatal development on adult social behavior and their relationship with the gut microbiota and the nervous system. The neural projections exist between the ascending colon and oxytocin neurons in the paraventricular nuclei (PVN), peripheral oxytocin levels and PVN neuron numbers decreased after cross-fostering, and sex-specific alteration in gut microbiota and its metabolites may be involved in social impairments and immune imbalances brought by cross-fostering via the gut-brain axis. Our findings also suggest that social cognitive impairment may result from a combination of PVN oxytocinergic neurons, gut microbiota, and metabolites.
Assuntos
Eixo Encéfalo-Intestino , Microbioma Gastrointestinal , Camundongos Endogâmicos BALB C , Neurônios , Ocitocina , Núcleo Hipotalâmico Paraventricular , Comportamento Social , Animais , Microbioma Gastrointestinal/fisiologia , Camundongos , Ocitocina/metabolismo , Masculino , Feminino , Núcleo Hipotalâmico Paraventricular/metabolismo , Eixo Encéfalo-Intestino/fisiologia , Neurônios/metabolismo , Encéfalo/metabolismo , Comportamento Animal/fisiologia , Colo/metabolismo , Colo/microbiologia , Animais Recém-NascidosRESUMO
BACKGROUND: Recent studies have identified empathy deficit as a core impairment and diagnostic criterion for people with autism spectrum disorders; however, the improvement of empathy focuses primarily on behavioural interventions without the target regulation. We sought to compare brain regions associated with empathy-like behaviours of fear and pain, and to explore the role of the oxytocin-oxytocin receptor system in fear empathy. METHODS: We used C57BL mice to establish 2 models of fear empathy and pain empathy. We employed immunofluorescence histochemical techniques to observe the expression of c-Fos throughout the entire brain and subsequently quantified the number of c-Fos-positive cells in different brain regions. Furthermore, we employed chemogenetic technology to selectively manipulate these neurons in Oxt-Cre-/+ mice to identify the role of oxytocin in this process. RESULTS: The regions activated by fear empathy were the anterior cingulate cortex, basolateral amygdala, nucleus accumbens, paraventricular nucleus (PVN), lateral habenula, and ventral and dorsal hippocampus. The regions activated by pain empathy were the anterior cingulate cortex, basolateral amygdala, nucleus accumbens, and lateral habenula. We found that increasing the activity of oxytocin neurons in the PVN region enhanced the response to fear empathy. This enhancement may be mediated through oxytocin receptors. LIMITATIONS: This study included only male animals, which restricts the broader interpretation of the findings. Further investigations on circuit function need to be conducted. CONCLUSION: The brain regions implicated in the regulation of fear and pain empathy exhibit distinctions; the activity of PVN neurons was positively correlated with empathic behaviour in mice. These findings highlight the role of the PVN oxytocin pathway in regulating fear empathy and suggest the importance of oxytocin signalling in mediating empathetic responses.
Assuntos
Empatia , Medo , Camundongos Endogâmicos C57BL , Neurônios , Ocitocina , Núcleo Hipotalâmico Paraventricular , Animais , Ocitocina/metabolismo , Masculino , Núcleo Hipotalâmico Paraventricular/metabolismo , Medo/fisiologia , Empatia/fisiologia , Neurônios/metabolismo , Camundongos , Receptores de Ocitocina/metabolismo , Proteínas Proto-Oncogênicas c-fos/metabolismo , Dor/fisiopatologia , Dor/psicologia , Camundongos TransgênicosRESUMO
BACKGROUND: Allergic rhinitis (AR) is a non-infectious inflammatory disease of the nasal mucosa mediated by IgE and involving a variety of immune cells such as mast cells. In previous studies, AR was considered as an isolated disease of the immune system. However, recent studies have found that the nervous system is closely related to the development of AR. Bidirectional communication between the nervous and immune systems plays an important role in AR. SUMMARY: The nervous system and immune system depend on the anatomical relationship between nerve fibers and immune cells, as well as various neurotransmitters, cytokines, inflammatory mediators, etc. to produce bidirectional connections, which affect the development of AR. KEY MESSAGES: This article reviews the impact of neuro-immune interactions in AR on the development of AR, including neuro-immune cell units.
RESUMO
PURPOSE: This study aims to raise awareness of the disparities in survival predictions among races in head and neck cancer (HNC) patients by developing and validating population-based prognostic models specifically tailored for Taiwanese and Asian populations. METHODS: A total of 49,137 patients diagnosed with HNCs were included from the Taiwan Cancer Registry (TCR). Six prognostic models, divided into three categories based on surgical status, were developed to predict both overall survival (OS) and cancer-specific survival using the registered demographic and clinicopathological characteristics in the Cox proportional hazards model. The prognostic models underwent internal evaluation through a tenfold cross-validation among the TCR Taiwanese datasets and external validation across three primary racial populations using the Surveillance, Epidemiology, and End Results database. Predictive performance was assessed using discrimination analysis employing Harrell's c-index and calibration analysis with proportion tests. RESULTS: The TCR training and testing datasets demonstrated stable and favorable predictive performance, with all Harrell's c-index values ≥ 0.7 and almost all differences in proportion between the predicted and observed mortality being < 5%. In external validation, Asians exhibited the best performance compared with white and black populations, particularly in predicting OS, with all Harrell's c-index values > 0.7. CONCLUSIONS: Survival predictive disparities exist among different racial groups in HNCs. We have developed population-based prognostic models for Asians that can enhance clinical practice and treatment plans.
Assuntos
Modelos Epidemiológicos , Neoplasias de Cabeça e Pescoço , Dados de Saúde Coletados Rotineiramente , Neoplasias de Cabeça e Pescoço/epidemiologia , Neoplasias de Cabeça e Pescoço/mortalidade , Taiwan , Análise de Sobrevida , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Grupos Raciais/estatística & dados numéricosRESUMO
OBJECTIVE: To investigate the effects of climb ladder and aerobic treadmill exercise on learning memory ability in diabetic rats and explore its possible mechanisms. METHODS: Forty male rats were randomly divided into normal control group (NC), diabetic control group (DC), diabetic loading ladder group (DL) and diabetic aerobic treadmill group (DA), diabetes was induced by a single intraperitoneal injection of STZ. In the evening, the DL group were trained three cycle (10 times/cycle) with weight-bearing climbing ladder, 2 min intervals, 6 days/week, lasted for six weeks. The DA group was trained on a motor-driven treadmill at a speed of 20 m/min (0 incline), 30 min/day, 6 days/week, lasted for six weeks. Morris water maze was used to detect the learning and memory ability of rats after modeling success and after exercise intervention. After the last water maze test, the rats were killed to obtain the hippocampus. RT-QPCR was used to detect the gene expressions of brain derived neurophic factor (BDNF), tropomyosin receptor kinase B (TrkB) and cAMP-response element binding protein (CREB). RESULTS: Compared with NC group, the expressions of BDNF and CREB gene in hippocampus of DC group and the learning and memory ability were significantly decreased. Compared with DC group, the expression of BDNF and CREB in hippocampus of DL and DA rats was significantly up-regulated and the learning ability was significantly increased. The TrkB gene of hippocampus in DL rats was significantly up-regulated and the spatial memory ability was significantly improved. Compared with the DA group, the TRKB and CREB genes in the hippocampus of DL group were significantly up-regulated. CONCLUSIONS: Aerobic treadmill exercise and weight-bearing ladder exercise have a positive effect on the learning ability of diabetic rats, while the weight-bearing ladder exercise improves the memory ability of diabetic rats better than aerobic exercise. These effects may be related to the up-regulation of BDNF/TrkB/CREB signaling pathway.