Characteristics of spike rate of peripheral sympathetic nervous signal in streptozotocin-induced diabetic rats.

Although streptozotocin-administered (STZ) rats were widely used as an experimental diabetic neuropathy model, sympathetic nerve activity (SNA) in STZ rats has not been microneurographically evaluated so far. In the present study, we investigated the multi-unit, compound sympathetic signal from the sciatic nerve of rats 3 weeks after the administration of streptozotocin, and compared the signal with that of normal (control) rats. After obtaining the sympathetic signal, glucose was intravenously administered to make a transient increase in the blood glucose level to cause SNA change. The sympathetic burst rate did not show any statistical difference between groups at steady state. Even after the glucose administration, it changed little in each group. On the other hand, the firing rate of action potentials (AP-rate) in STZ group was significantly lower than that in control group before glucose administration (p<0.05). In addition, AP-rate was increased in control animals after glucose administration, but not in STZ rats. The results suggest a lower sympathetic tone and poorer response to glucose load under high blood glucose and low plasma insulin condition, and that the AP-rate may be useful for the evaluation of microneurographically measured, peripheral sympathetic activity.

Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats.

Reconstructed myocardial tissue still does not have enough pulsatile contraction. It is well known that fetal and mature neonatal cardiomyocytes utilize glucose and lipid, respectively, as their energy substrates, and that cultured ones mainly use glucose in spite of their age comparable to neonate ones, probably due to insufficient supply of lipids from culture medium. In the present study, we compared 7 saturated, 6 monounsaturated, and 11 polyunsaturated fatty acid contents in cultured cardiomyocytes (Cul group) with those in fetal (Fet group, approximately 17 d after impregnation) and neonatal (Neo group, 9 d old) rats, where the age of the Cul cells were set nearly equal to the Neo ones. Saturated fatty acid contents in the Cul group were generally lower than those in the Fet group and were close to those in the Neo group, except for C12:0 of which content was highest in the Neo group. Monounsaturated fatty acid contents in the Cul group were generally lower than those in the Fet group but similar to or higher than those in the Neo group, except for C24:1n-9 of which content was again highest in the Neo group. In contrast, most of polyunsaturated fatty acid (PUFA) contents in the Cul group appeared lower than those in both the Fet and Neo groups, and differences in 5 of 10 detected PUFAs were significant between the Cul and Neo groups. The results suggest that PUFA contents in cultured cardiomyocytes might be insufficient to exert enough contractile ability. In conclusion, it could be necessary for cultured cardiomyocytes to uptake more lipid; PUFAs in particular.

Site dependency of fatty acid composition in adipose triacylglycerol in rats and its absence as a result of high-fat feeding.

It is currently believed that metabolic syndrome, in general, and type 2 diabetes mellitus, in particular, depend more on visceral than on subcutaneous adipose tissue. However, the relationship between insulin resistance and fatty acid composition in visceral and subcutaneous adipose tissues remains to be clarified. In the present study, we extracted the triacylglycerol from visceral (epididymis and mesentery) and subcutaneous adipose tissues in normal and insulin-resistant, high-fat-fed (HFF) rats and determined the composition of each fatty acid. The concentrations of palmitoleic, docosapentaenoic, docosahexaenoic, dihomo-γ-linolenic, arachidonic, and docosatetraenoic acids were higher in epididymal adipose tissue than in mesenteric and subcutaneous adipose tissues; but no significant differences were detected between mesenteric and subcutaneous tissues in the normal group or among all the sites in the HFF rats. In the HFF group, stearic and oleic acid concentrations were higher, whereas n-3 and n-6 polyunsaturated ones were lower, than those in the normal group. Palmitoleic acid and some n-3 and n-6 polyunsaturated fatty acid compositions in adipose tissue triacylglycerol depend on anatomical location, which may affect the properties and/or function of adipose tissues. These results at least in part suggest that the properties of adipose tissue are difficult to distinguish based only on their "visceral" or "subcutaneous" sites. In addition, the absence of site dependence and/or difference in balance among saturated, monounsaturated, and polyunsaturated fatty acids may play an important role in the development of insulin resistance in the HFF rats.