RESUMO
Adhatoda vasica and Calotropis procera species were investigated as a resource for new diverse pharmacological agents including B complex, individual total phenolic compounds and antioxidants for curing and treatments of many infectious diseases in human through advanced analytical methods. These plants are abundant in Khyber Pukhtoon Khawa, Pakistan as well as in all over the world and famous for their unique medicinal importance. These herbaceous species are so far used for animals curing while current exploration of these species showed that these species are a precious resource of various compounds which can be employed in the formation of different drugs. The results showed that the leaf and flower extracts of Adhatoda vasica and leaf extract of Calotropis procera contained higher contents of bioactive compounds. The chemical analysis of the samples resulted in higher values of total phenolic compounds (71.32mg GAE/g), total antioxidants (651% DPPH inhibition), the enzyme catalase (4716µg/g), ash content (16.72%) and pH values in the Calotropis procera, whereas the total carotenoids (1987mg/100g), the enzymes, superoxide dismutase (4566µg/g) and peroxidase (1322µg/g) were higher in leaves of Adhatoda vasica. The flower extract of the Adhatoda vasica was rich in the flavonoids (0.87mg/100g) and organic matter (89.99%) as compared to Calotropis procera. The obtained data for each parameter was interpreted by applying Complete Randomized Design (CRD) along with factorial arrangements. The mean comparison was performed using LSD test at 5% probability level. The presence of these phytochemicals may lead to the conclusion that these herbal plants have the potential for formation of new drugs and can be used as herbal medicine for treatment of different cancer and viral diseases. These compounds are also useful in the treatment of the tumor.