RESUMO
Magnetic skyrmions are nanoscale topological spin structures offering great promise for next-generation information storage technologies. The recent discovery of sub-100-nm room-temperature (RT) skyrmions in several multilayer films has triggered vigorous efforts to modulate their physical properties for their use in devices. Here we present a tunable RT skyrmion platform based on multilayer stacks of Ir/Fe/Co/Pt, which we study using X-ray microscopy, magnetic force microscopy and Hall transport techniques. By varying the ferromagnetic layer composition, we can tailor the magnetic interactions governing skyrmion properties, thereby tuning their thermodynamic stability parameter by an order of magnitude. The skyrmions exhibit a smooth crossover between isolated (metastable) and disordered lattice configurations across samples, while their size and density can be tuned by factors of two and ten, respectively. We thus establish a platform for investigating functional sub-50-nm RT skyrmions, pointing towards the development of skyrmion-based memory devices.
RESUMO
The Raman spectra of Si nanocrystals are studied as a function of nanocrystal diameter using pseudopotential density functional theory and the Placzek approximation. Our calculations reproduce the redshift and broadening of the optical Raman peak with decreasing nanocrystal size, and calculated peak frequencies show good agreement with experimental values. We also find that a surface induced softening of vibrational modes is largely responsible for the Raman redshift, with relaxation of momentum conservation playing only a minor role.
RESUMO
Parasitic nematodes infect billions of people world-wide, often causing chronic infections associated with high morbidity. The greatest interface between the parasite and its host is the cuticle surface, the outer layer of which in many species is covered by a carbohydrate-rich glycocalyx or cuticle surface coat. In addition many nematodes excrete or secrete antigenic glycoconjugates (ES antigens) which can either help to form the glycocalyx or dissipate more extensively into the nematode's environment. The glycocalyx and ES antigens represent the main immunogenic challenge to the host and could therefore be crucial in determining if successful parasitism is established. This review focuses on a few selected model systems where detailed structural data on glycoconjugates have been obtained over the last few years and where this structural information is starting to provide insight into possible molecular functions.
Assuntos
Infecções por Nematoides/imunologia , Animais , Sequência de Carboidratos , Epitopos/metabolismo , Hexoses/metabolismo , Dados de Sequência Molecular , Infecções por Nematoides/parasitologia , Toxocara/imunologiaRESUMO
Previous studies have shown that polymerized [14C]arabinan can be synthesized from polyprenylphosphate-[14C]arabinose by the particulate enzymes of Mycobacterium smegmatis [R.E. Lee, K. Mikusová, P.J. Brennan and G.S Besra (1995) J. Am. Chem. Soc. 117, 11829-11832]. In the present investigation, the [14C]arabinan product was biochemically characterized. Sizing chromatography revealed a molecular weight consistent with that expected from mature arabinan. Digestion of the [14C]arabinan with a mixture of arabinases produced oligo[14C]arabinoside fragments including hexa[14C]arabinoside and tetra[14C]arabinoside which originated from the non-reducing terminal regions of the polymer, and di[14C]arabinoside from the internal regions of the polymer. These arabinoside fragments represent the major known structural motifs that comprise the arabinan segment of arabinogalactan and lipoarabinomannan. The presence of [14C]arabinose in both the internal and external regions of the [14C]arabinan suggests that polyprenylphosphate-arabinose is the major, and perhaps the only, donor of arabinosyl residues in mycobacteria.
Assuntos
Mycobacterium/enzimologia , Polissacarídeos/biossíntese , Parede Celular/enzimologia , Glicosídeo Hidrolases , Peso Molecular , Pentosiltransferases/isolamento & purificação , Pentosiltransferases/metabolismo , Polímeros , Polissacarídeos/químicaRESUMO
Epidermal growth factor receptor (EGFR) regulates multiple signaling cascades essential for cell proliferation, growth and differentiation. Using a genetic approach, we found that Drosophila FERM and PDZ domain-containing protein tyrosine phosphatase, dPtpmeg, negatively regulates border cell migration and inhibits the EGFR/Ras/mitogen-activated protein kinase signaling pathway during wing morphogenesis. We further identified EGFR pathway substrate 15 (Eps15) as a target of dPtpmeg and its human homolog PTPN3. Eps15 is a scaffolding adaptor protein known to be involved in EGFR endocytosis and trafficking. Interestingly, PTPN3-mediated tyrosine dephosphorylation of Eps15 promotes EGFR for lipid raft-mediated endocytosis and lysosomal degradation. PTPN3 and the Eps15 tyrosine phosphorylation-deficient mutant suppress non-small-cell lung cancer cell growth and migration in vitro and reduce lung tumor xenograft growth in vivo. Moreover, depletion of PTPN3 impairs the degradation of EGFR and enhances proliferation and tumorigenicity of lung cancer cells. Taken together, these results indicate that PTPN3 may act as a tumor suppressor in lung cancer through its modulation of EGFR signaling.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Movimento Celular , Proliferação de Células , Receptores ErbB/metabolismo , Neoplasias Pulmonares/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 3/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Animais Geneticamente Modificados , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular , Linhagem Celular Tumoral , Endocitose , Feminino , Células HEK293 , Humanos , Immunoblotting , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Masculino , Microdomínios da Membrana/metabolismo , Camundongos Endogâmicos BALB C , Camundongos Nus , Microscopia Confocal , Mutação , Fosforilação , Proteína Tirosina Fosfatase não Receptora Tipo 3/genética , Interferência de RNA , Transplante HeterólogoRESUMO
The variant surface glycoprotein of the bloodstream form of Trypanosoma brucei is known to be glycosylated with a range of structures including high mannose and complex types. In contrast, glycosylation in the procyclic form of the parasite appears to be restricted to a single Man(5)GlcNAc(2) structure, as found on its procyclin. To gain a better insight into the developmentally regulated glycosylation pattern, we have structurally defined the full range of N-linked glycans made by the procyclic trypanosomes, as well as two previously described glycosylation mutants generated under Con A selection. It was found that the wild type procyclic cells could synthesize a full range of high mannose type structures from Man(5)GlcNAc(2) to Man(9)GlcNAc(2), with Man(5)GlcNAc(2) as the major component. In contrast, the two mutants mainly synthesized a truncated Man(4)GlcNAc(2) structure, Man alpha 1-3Man alpha 1-6(Man alpha1-3)Man be ta 1-4 GlcNAc beta 1-4GlcNAc, a significant portion of which was further extended by a single GlcNAc to form GlcNAc-Man(4)GlcNAc(2) and a single N-acetyllactosamine unit at the 3-arm position to form Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3(Man al pha 1- 3Man alpha 1-6)Man beta 1-4G lcNAc beta 1-4GlcNAc. The results suggest that the procyclic trypanosomes could be induced by Con A selection to synthesize limited hybrid type structures, but in general do not further process their N-linked glycans into multiantennary complex types as the blood stream forms do.
Assuntos
Concanavalina A/farmacologia , Glicopeptídeos/química , Oligossacarídeos/química , Polissacarídeos/biossíntese , Polissacarídeos/química , Trypanosoma brucei brucei/metabolismo , Animais , Asparagina , Configuração de Carboidratos , Sequência de Carboidratos , Glicosilação , Manose/análise , Dados de Sequência Molecular , Mutação , Oligossacarídeos/biossíntese , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Trypanosoma brucei brucei/efeitos dos fármacos , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/crescimento & desenvolvimentoRESUMO
Infection by the tapeworm Echinococcus granulosus in the intermediate host results in the development of a hydatid cyst which contains the protoscoleces within a fluid-filled cavity enclosed by the bilayered cyst membrane. N-glycans were enzymatically released from crude extracts of homogenates of hydatid cyst membranes and protoscoleces and their structures were defined by high sensitivity fast atom bombardment mass spectrometry in conjunction with sequential exoglycosidase digestions. The major N-glycans from the cyst membrane were found to be non-charged structures having complex-type antennae and core fucosylation. The antennae are either truncated at the first N-acetylglucosamine or are extended with beta-galactose to form N-acetyllactosamine (lacNAc). A significant proportion of the lacNAc backbones are capped by alpha-galactose. The resulting Gal alpha-Gal beta-terminal structures may account for the earlier observation that antibodies against the blood group P1 epitope recognise components of hydatid cyst extracts. The complex-type N-glycans identified in the protoscoleces extracts were the same as the neutral structures found in the cyst membrane but a small proportion of high mannose structures and truncated di- and trimannosyl core structures were also identified. Sialylated N-glycans were identified as minor constituents of the cyst membrane preparation but were not observed in protoscoleces extracts. Whether the sialylated glycans are host derived or endogenously synthesized by the parasite remains to be established. This is the first reported structural analysis of N-glycans from cestodes and provides new insights into protein glycosylation in helminths.
Assuntos
Equinococose/metabolismo , Equinococose/patologia , Echinococcus/química , Polissacarídeos/química , Animais , Antígenos de Helmintos/química , Antígenos de Helmintos/imunologia , Sequência de Carboidratos , Carboidratos/análise , Equinococose/imunologia , Echinococcus/imunologia , Eletroforese/métodos , Corantes Fluorescentes , Membranas/química , Dados de Sequência Molecular , Polissacarídeos/imunologia , Espectrometria de Massas de Bombardeamento Rápido de Átomos/métodosRESUMO
The major excretory-secretory product of the rodent filarial nematode Acanthocheilonema viteae is a 62 kDa glycoprotein (ES-62), which has phosphorylcholine, attached to the N-linked carbohydrates. In this paper, we describe structural studies of N-glycans released from ES-62 by peptide N-glycosidase F. Three major classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and four additional N-acetylglucosamine resides. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine. The implications of these results with respect to the probable glycosylation pathways operating in A. viteae are discussed.
Assuntos
Dipetalonema/química , Glicoproteínas/química , Proteínas de Helminto/química , Oligossacarídeos/química , Fosforilcolina , Animais , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas , Ácido Fluorídrico , Modelos Biológicos , Modelos Moleculares , Dados de Sequência Molecular , Oligossacarídeos/biossíntese , Análise de Sequência , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
Derivatisation/FABMS strategies applicable to the structure analysis of low microgram quantities of heparin-derived oligosaccharides are described. Negative and positive FAB data from permethyl derivatives and positive FAB data from the products of subsequent methanolysis are reported for sulfated tetrasaccharides prepared by nitrous acid degradation of heparin. The preparation and FAB behaviour of acetylated derivatives of sulfated oligosaccharides are described for the first time, and the stability of the sulfate groups to base-catalysed acetylation is demonstrated. The acetylation/FABMS methodology, which yields high quality data, shows promise for the characterisation of a wide range of sulfated glycoconjugates.
Assuntos
Heparina/química , Oligossacarídeos/análise , Acetilação , Sequência de Carboidratos , Desaminação , Metanol/química , Metilação , Dados de Sequência Molecular , Estrutura Molecular , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
Phage resistance and apparent lysogenization of Mycobacterium smegmatis due to infection with mycobacteriophage D29 results in the emergence of new variations of the pyruvylated, acylated trehaloses described by Saadat and Ballou, J. Biol. Chem. 258 (1983) 1813-1818. Thin-layer chromatography of the glycolipids from two strains of phage-resistant M. smegmatis (mc(2)22 and mc(2)11) and comparison with those from phage-sensitive strains revealed a new, more mobile glycolipid in each case. The structures of these acyltrehalose-containing lipooligosaccharides were elucidated by a combination of gas-liquid chromatography-mass spectrometry, methylation analysis, 1H and 13C NMR spectroscopy, and fast-atom bombardment mass spectrometry. The glycolipid from M. smegmatis mc(2)22 is beta-D-Glcp-(1-->3)-4,6-O-(1-methoxycarbonylethylidene)-beta-D-Glc p-(1-->4)- beta-D-Glcp-(1-->6)-2-O-acyl-alpha-D-Glcp-(<==>1)-3,4-O-acyl-alpha-D-Glc p and that from M. smegmatis mc(2)11 is 4,6-O-(1-methoxycarbonylethylidene)-3-O-Me-beta-D-Glcp-(1-->3)-4,6 -O- (1-methoxycarbonylethylidene)-beta-D-Glcp-(1-->4)-beta-D-Glcp-(1-- >6)-2-O-acyl- alpha-D-Glcp-(1<==>1)-3,4-di-O-acyl-alpha-D-Glcp. These differ from the original pyruvylated glycolipids of Saadat and Ballou in the extent of their O-acylation and O-methylation. The findings are the first example of the definition of a chemical basis for phage resistance and presumed lysogeny in mycobacteria, and show parallels to related changes in gram-negative enteric bacteria.
Assuntos
Glicolipídeos/química , Micobacteriófagos/crescimento & desenvolvimento , Mycobacterium/química , Trealose/análogos & derivados , Acilação , Sequência de Carboidratos , Ácidos Graxos/análise , Variação Genética , Lipopolissacarídeos/química , Dados de Sequência Molecular , Mycobacterium/genética , Piruvatos , Ácido Pirúvico , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
Four kinds of sulfated trisaccharides resistant to chondroitinase ABC were isolated after chondroitinase B or ABC treatment of dermatan sulfate or various chondroitin sulfate isomers, respectively. Their composition was determined by chemical analysis and fast atom bombardment-mass spectrometry. Their structures were characterized by chondroitinase ACII digestion in conjunction with HPLC, and 500-MHz one- and two-dimensional 1H NMR spectroscopy. All the four trisaccharides have in common the core saccharide sequence, alpha-L-delta 4,5HexpA-(1-->3)-beta-D-GalpNAc-(1-->4)-D-GlcpA. A monosulfated component isolated from shark scapular cartilage chondroitin sulfate C or bovine aorta dermatan sulfate was elucidated as alpha-L-delta 4,5HexpA-(1-->3)-beta-D-GalpNAc6SO3(-)-(1-->4)-D-GlcpA or alpha-L-delta 4,5HexpA-(1-->3)-beta-D-GalpNAc4SO3(-)-(1-->4)-D-GlcpA , respectively. A disulfated component obtained from shark scapular cartilage chondroitin sulfate C or squid cartilage chondroitin sulfate E was identified as alpha-L-delta 4,5HexpA2SO3(-)-(1-->3)-beta-D-GalpNAc6SO3(-)-(1-->4)-D-G lcpA or alpha-L-delta 4,5HexpA-(1-->3)-beta-D-GalpNAc4SO3(-)6SO3(-)-(1-->4)- D-GlcpA, respectively. These trisaccharides are derived from the reducing termini of the parent polysaccharides. Some of the trisaccharides could be derived from the reducing termini exposed by the peeling reaction during the alkaline treatment while some others may represent the cleavage sites exposed by tissue endo-beta-D-glucuronidase(s), indicating the presence of such enzyme(s) which may release chondroitin/dermatan sulfate fragments from proteoglycans.
Assuntos
Condroitina Liases/metabolismo , Sulfatos de Condroitina/química , Dermatan Sulfato/química , Trissacarídeos/química , Animais , Aorta/química , Sequência de Carboidratos , Bovinos , Sulfatos de Condroitina/metabolismo , Dermatan Sulfato/metabolismo , Glucuronidase/metabolismo , Glicosaminoglicanos/metabolismo , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Oxirredução , Proteoglicanas/metabolismo , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
We have provided an account of the progress we and others have made over the last decade on the structural characterization of glycans from parasitic helminths. We hope to have illustrated a few principles and patterns governing helminth glycosylation, as well as the experimental approaches adopted and their associated strengths and limitations. Schistosomes remain the best studied systems but are still punctuated with gaps of knowledge. An important theme developed here is the regulated developmental stage-specific expression of various glycan epitopes and their interplay with immediate host environments for successful parasitism. It is anticipated that more novel or unusual structures will continuously be uncovered in the future and that despite many difficulties, current analytical techniques should be well up to meet the challenge in at least elucidating the major or key glycoconjugates from each of the diverse range of worms. The bottle neck will in fact reside in finding suitable experimental models to test their putative immunobiological functions from which the intricate host-parasite interactions can be delineated and rational vaccine design be achieved. The glycobiology of parasitic helminths is an area waiting to be more fully explored and the rewards should be sweet.
Assuntos
Glicoconjugados/química , Helmintos/química , Animais , Antígenos de Helmintos/química , Sequência de Carboidratos , Feminino , Glicoconjugados/imunologia , Glicoconjugados/isolamento & purificação , Glicoproteínas/química , Glicoproteínas/imunologia , Glicosilação , Proteínas de Helminto/química , Proteínas de Helminto/imunologia , Helmintos/imunologia , Helmintos/patogenicidade , Dados de Sequência Molecular , Estrutura Molecular , Nematoides/química , Nematoides/imunologia , Óvulo/química , Óvulo/imunologia , Schistosoma mansoni/química , Schistosoma mansoni/imunologiaAssuntos
Glicoconjugados/química , Glicoproteínas/química , Glicosaminoglicanos/química , Polissacarídeos/química , Acetilação , Configuração de Carboidratos , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Glicopeptídeos/química , Indicadores e Reagentes , Espectrometria de Massas/métodos , Metilação , Dados de Sequência Molecular , Monossacarídeos/análise , Monossacarídeos/química , Proteínas Recombinantes/química , Espectrometria de Massas de Bombardeamento Rápido de Átomos/métodosAssuntos
Glicolipídeos/química , Glicopeptídeos/química , Lipopolissacarídeos/química , Mycobacterium/química , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Molecular , Peptídeos/química , Piruvatos/química , Serina/análogos & derivados , Serina/química , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Trealose/análogos & derivados , Trealose/químicaRESUMO
STM studies on K(x)C(60) monolayers reveal new behavior over a wide range of the phase diagram. As x increases from 3 to 5 K(x)C(60) monolayers undergo metal-insulator-metal reentrant phase transitions and exhibit a variety of novel orientational orderings, including a complex 7-molecule, pinwheel-like structure. The proposed driving mechanism for the orientational ordering is the lowering of electron kinetic energy by maximizing the overlap of neighboring molecular orbitals. In insulating (metallic) K(x)C(60) this gives rise to orbital versions of the superexchange (double-exchange) interaction.
Assuntos
Metais , Elementos de Transição , Elétrons , Metais/químicaRESUMO
We present a low-temperature scanning tunneling microscopy (STM) study of K(x)C60 monolayers on Au(111) for 3 < or = x < or = 4. The STM spectrum evolves from one that is characteristic of a metal at x = 3 to one that is characteristic of an insulator at x = 4. This electronic transition is accompanied by a dramatic structural rearrangement of the C60 molecules. The Jahn-Teller effect, a charge-induced mechanical deformation of molecular structure, is directly visualized in the K4C60 monolayer at the single-molecule level. These results, along with theoretical analyses, provide strong evidence that the transition from metal to insulator in K(x)C60 monolayers is caused by the Jahn-Teller effect.
RESUMO
We have measured the elastic and inelastic tunneling properties of isolated Gd@C(82) molecules on Ag(001) using cryogenic scanning tunneling spectroscopy. We find that the dominant inelastic channel is spatially well localized to a particular region of the molecule. Ab initio pseudopotential density-functional theory calculations indicate that this channel arises from a vibrational cage mode. We further show that the observed inelastic tunneling localization is explained by strong localization in the molecular electron-phonon coupling to this mode.
RESUMO
This paper describes a sensitive strategy employing fast atom bombardment mass spectrometry (FAB-MS) for defining the anomeric configurations of pyranose sugars in oligosaccharides. The method, which is applicable to mixtures of reduced or unreduced oligosaccharides, is based upon FAB-MS analyses of deuteroacetylated derivatives before and after oxidation with chromium trioxide. This reagent, whose potential value in carbohydrate chemistry was first recognized by Angyal and which was subsequently more fully exploited by Lindberg, oxidizes beta-pyranosides to keto-esters leaving alpha-pyranosides largely intact. In this paper we show that the products of chromium trioxide oxidation can be successfully analysed at the microgram level using FAB-MS. The molecular and fragment ions produced in the FAB experiment define the number of sites oxidized and their location in the sequence. For samples which fragment poorly we describe a mild methanolysis procedure, compatible with FAB-MS, which preferentially cleaves the esters formed during the oxidation. Incorporation of an acetolysis step prior to oxidation permits analyses of polysaccharides. This oxidation/FAB-MS strategy should prove valuable in structural analyses of a wide range of biologically important carbohydrates which cannot be isolated in sufficient quantities to permit nuclear magnetic resonance studies.
Assuntos
Carboidratos/química , Oligossacarídeos/química , Configuração de Carboidratos , Sequência de Carboidratos , Glicoforinas/química , Indicadores e Reagentes , Isomerismo , Dados de Sequência Molecular , Estrutura Molecular , Espectrometria de Massas de Bombardeamento Rápido de Átomos/métodosRESUMO
One of the most important opportunistic pathogens associated with acquired immunodeficiency syndrome (AIDS) is the M. avium complex. M. avium infections are found in up to 70% of individuals in advanced stages of AIDS. It is apparent that M. avium can replicate in host macrophages and persist for long periods. This group of mycobacteria are distinguished by the presence of unique, highly antigenic, surface-located lipids known as the glycopeptidolipids (GPLs). The GPLs are the chemical basis of the 31 distinct serovars of the M. avium complex, and have also been identified in some other species. The M. avium lipids are immunosuppressive and can induce a variety of cytokines that affect general host responses. Despite extensive chemical characterization of the structures of these GPLs, much work is needed to elucidate the molecular mechanism involved in this complex glycosylation pathway and its genetic basis. The challenges for the future lie in explaining the roles of these copious products in the intracellular life and infectivity of mycobacteria. The intention of our review is to offer a concise account of the structures of the M. avium lipids, their putative roles in the host responses, bacterial physiology and pathogenesis, particularly in immunocompromised patients such as those infected with human immunodeficiency virus (HIV). Advances in chemical synthesis of the various haptenic oligosaccharides are also given to demonstrate how these have helped to define the immunogenic determinants. We believe that future research should involve the creation of conditional mutants defective in these lipids for both functional and biosynthesis studies which will complement biological assays using chemically defined or modified neoglycoconjugates.
Assuntos
Antígenos de Bactérias/química , Antígenos de Superfície/química , Glicoconjugados/química , Mycobacterium/química , Adjuvantes Imunológicos , Antígenos de Bactérias/imunologia , Antígenos de Superfície/imunologia , Sequência de Carboidratos , Glicoconjugados/imunologia , Infecções por HIV/imunologia , Haptenos/química , Haptenos/imunologia , Dados de Sequência Molecular , Mycobacterium/classificação , Mycobacterium/imunologia , Complexo Mycobacterium avium/química , Complexo Mycobacterium avium/imunologia , Oligossacarídeos/químicaRESUMO
Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integrity although the focus has largely been on the arabinan motifs and the terminal capping function. It has recently been shown that the mannose caps may be involved not only in attenuating host immune response, but also in mediating the binding of mycobacteria to and subsequent entry into macrophages. This may further be linked to an intracellular trafficking pathway through which LAM is thought to be presented by CD1 to subsets of T-cells. The implication of LAM as major histocompatibility complex (MHC)-independent T-cell epitope and the ensuing immune response is an area of intensive studies. Another recent focus of research is the biosynthesis of arabinan which has been shown to be inhibitable by the anti-tuberculosis drug, ethambutol. The phenomenon of truncated LAM as synthesized by ethambutol resistant strains provides an invaluable handle for dissecting the array of arabinosyltransferases involved, as well as generating much needed structural variants for further structural and functional studies. It is hoped that with more systematic investigations based on clinical isolates and human cell lines, the true significance of LAM in the immunopathogenesis of tuberculosis and leprosy can eventually be explained.